Nerve growth factor is an autocrine survival factor for memory B lymphocytes.


:Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD "virgin" B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific IgG in mice immunized with tetanus toxoid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.






Torcia M,Bracci-Laudiero L,Lucibello M,Nencioni L,Labardi D,Rubartelli A,Cozzolino F,Aloe L,Garaci E




Has Abstract


1996-05-03 00:00:00
















  • Oncogenic activity of the c-Myc protein requires dimerization with Max.

    abstract::c-Myc (Myc) and Max proteins dimerize and bind DNA through basic-helix-loop-helix-leucine zipper motifs (b-HLH-LZ). Using a genetic approach, we demonstrate that binding to Max is essential for Myc transforming activity and that Myc homodimers are inactive. Mutants of Myc and Max that bind efficiently to each other bu...


    pub_type: 杂志文章


    authors: Amati B,Brooks MW,Levy N,Littlewood TD,Evan GI,Land H

    更新日期:1993-01-29 00:00:00

  • The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis.

    abstract::The transition from the juvenile to the adult phase of shoot development in plants is accompanied by changes in vegetative morphology and an increase in reproductive potential. Here, we describe the regulatory mechanism of this transition. We show that miR156 is necessary and sufficient for the expression of the juven...


    pub_type: 杂志文章


    authors: Wu G,Park MY,Conway SR,Wang JW,Weigel D,Poethig RS

    更新日期:2009-08-21 00:00:00

  • Chemical genetics: where genetics and pharmacology meet.

    abstract::Genetics and pharmacology can elicit surprisingly different phenotypes despite targeting the same protein. This Essay explores these unexpected differences and their implications for biology and medicine. ...


    pub_type: 杂志文章


    authors: Knight ZA,Shokat KM

    更新日期:2007-02-09 00:00:00

  • Overlapping activators and repressors delimit transcriptional response to receptor tyrosine kinase signals in the Drosophila eye.

    abstract::Regulated transcription of the prospero gene in the Drosophila eye provides a model for how gene expression is specifically controlled by signals from receptor tyrosine kinases. We show that prospero is controlled by signals from the EGF receptor DER and the Sevenless receptor. A direct link is established between DER...


    pub_type: 杂志文章


    authors: Xu C,Kauffmann RC,Zhang J,Kladny S,Carthew RW

    更新日期:2000-09-29 00:00:00

  • Protein traffic between distinct plasma membrane domains: isolation and characterization of vesicular carriers involved in transcytosis.

    abstract::We have isolated a population of vesicular carriers involved in the transport (transcytosis) of proteins from the basolateral to the apical plasma membrane of hepatocytes. The obtained fraction was enriched in compartments containing known transcytosed proteins and depleted in elements of the secretory pathway, Golgi ...


    pub_type: 杂志文章


    authors: Sztul E,Kaplin A,Saucan L,Palade G

    更新日期:1991-01-11 00:00:00

  • The primary sequence of rabbit alpha-globin mRNA.

    abstract::The rabbit alpha-globin DNA insertion in the chimeric plasmid pHb 72 (Liu et al., 1977) has been sequenced by the method of Maxam and Gilbert (1977). This has enabled us to determine the messenger RNA(mRNA) sequence beginning in the 5' untranslated region 9 nucleotides before the initiation codon and extending through...


    pub_type: 杂志文章


    authors: Heindell HC,Liu A,Paddock GV,Studnicka GM,Salser WA

    更新日期:1978-09-01 00:00:00

  • Isolation and characterization of sigma(70)-retaining transcription elongation complexes from Escherichia coli.

    abstract::sigma(70) subunit is thought to be released from the core RNA polymerase (RNAP) upon the transition from initiation to elongation or shortly afterward. Here, we identify a population of RNAP from E. coli that retains sigma(70) throughout elongation. The relative amount of this population appears to depend on cellular ...


    pub_type: 杂志文章


    authors: Bar-Nahum G,Nudler E

    更新日期:2001-08-24 00:00:00

  • Isolation of the bovine and human genes for Müllerian inhibiting substance and expression of the human gene in animal cells.

    abstract::We have isolated the bovine and human genes for Müllerian inhibiting substance (MIS), a testicular glycoprotein that causes regression of the Müllerian duct during development of the male embryo. The mRNA sequence of bovine MIS, determined from an analysis of cDNA and genomic clones, codes for a protein of 575 amino a...


    pub_type: 杂志文章


    authors: Cate RL,Mattaliano RJ,Hession C,Tizard R,Farber NM,Cheung A,Ninfa EG,Frey AZ,Gash DJ,Chow EP

    更新日期:1986-06-06 00:00:00

  • The CaaX motif of lamin A functions in conjunction with the nuclear localization signal to target assembly to the nuclear envelope.

    abstract::While the nuclear lamin proteins (A, B, and C) assemble specifically at the surface of the nuclear membrane, their sequences do not reveal stretches of hydrophobic amino acids that might explain their association with the nuclear membranes. However, the A and B lamin proteins possess Ras-like C-terminal CaaX sequence ...


    pub_type: 杂志文章


    authors: Holtz D,Tanaka RA,Hartwig J,McKeon F

    更新日期:1989-12-22 00:00:00

  • IkappaB kinase promotes tumorigenesis through inhibition of forkhead FOXO3a.

    abstract::Nuclear exclusion of the forkhead transcription factor FOXO3a by protein kinase Akt contributes to cell survival. We investigated the pathological relationship between phosphoylated-Akt (Akt-p) and FOXO3a in primary tumors. Surprisingly, FOXO3a was found to be excluded from the nuclei of some tumors lacking Akt-p, sug...


    pub_type: 杂志文章


    authors: Hu MC,Lee DF,Xia W,Golfman LS,Ou-Yang F,Yang JY,Zou Y,Bao S,Hanada N,Saso H,Kobayashi R,Hung MC

    更新日期:2004-04-16 00:00:00

  • At least 104 nucleotides are transposed from the 5' terminus of the avian sarcoma virus genome to the 5' termini of smaller viral mRNAs.

    abstract::Cells producing avian sarcoma virus (ASV) contain at least three virus-specific mRNAs, two of which are encoded within the 3' half of the viral genome. Each of these viral RNAs can hybridize with single-stranded DNA(cDNA5') that is complementary to a sequence of 101 nucleotides found at the 5' terminus of the ASV geno...


    pub_type: 杂志文章


    authors: Cordell B,Weiss SR,Varmus HE,Bishop JM

    更新日期:1978-09-01 00:00:00

  • Transformation of cells by an inhibitor of phosphatases acting on phosphotyrosine in proteins.

    abstract::Vanadate has been shown to inhibit phosphatases that remove phosphate groups from phosphotyrosine in cell-free systems. Addition of vanadate to the culture medium of NRK-1 cells resulted in a maximal 40-fold increase in the level of phosphotyrosine in cell protein. Also, vanadate induced transformation as evidenced by...


    pub_type: 杂志文章


    authors: Klarlund JK

    更新日期:1985-07-01 00:00:00

  • Cryo-EM Structure of the Human Cannabinoid Receptor CB2-Gi Signaling Complex.

    abstract::Drugs selectively targeting CB2 hold promise for treating neurodegenerative disorders, inflammation, and pain while avoiding psychotropic side effects mediated by CB1. The mechanisms underlying CB2 activation and signaling are poorly understood but critical for drug design. Here we report the cryo-EM structure of the ...


    pub_type: 杂志文章


    authors: Xing C,Zhuang Y,Xu TH,Feng Z,Zhou XE,Chen M,Wang L,Meng X,Xue Y,Wang J,Liu H,McGuire TF,Zhao G,Melcher K,Zhang C,Xu HE,Xie XQ

    更新日期:2020-02-20 00:00:00

  • Cis-acting induction of adenovirus transcription.

    abstract::At early times after adenovirus infection, transcription from all early viral promoters requires the expression of a 289 amino acid phosphoprotein encoded in the pre-early transcription unit E1A. Mutations in E1A result in a marked delay in the induction of early viral transcription. To distinguish between possible mo...


    pub_type: 杂志文章


    authors: Gaynor RB,Berk AJ

    更新日期:1983-07-01 00:00:00

  • Quiescence Modulates Stem Cell Maintenance and Regenerative Capacity in the Aging Brain.

    abstract::The function of somatic stem cells declines with age. Understanding the molecular underpinnings of this decline is key to counteract age-related disease. Here, we report a dramatic drop in the neural stem cells (NSCs) number in the aging murine brain. We find that this smaller stem cell reservoir is protected from ful...


    pub_type: 杂志文章


    authors: Kalamakis G,Brüne D,Ravichandran S,Bolz J,Fan W,Ziebell F,Stiehl T,Catalá-Martinez F,Kupke J,Zhao S,Llorens-Bobadilla E,Bauer K,Limpert S,Berger B,Christen U,Schmezer P,Mallm JP,Berninger B,Anders S,Del Sol A,Marc

    更新日期:2019-03-07 00:00:00

  • A pericellular collagenase directs the 3-dimensional development of white adipose tissue.

    abstract::White adipose tissue (WAT) serves as the primary energy depot in the body by storing fat. During development, fat cell precursors (i.e., preadipocytes) undergo a hypertrophic response as they mature into lipid-laden adipocytes. However, the mechanisms that regulate adipocyte size and mass remain undefined. Herein, we ...


    pub_type: 杂志文章


    authors: Chun TH,Hotary KB,Sabeh F,Saltiel AR,Allen ED,Weiss SJ

    更新日期:2006-05-05 00:00:00

  • TEL1, a gene involved in controlling telomere length in S. cerevisiae, is homologous to the human ataxia telangiectasia gene.

    abstract::Yeast chromosomes terminate in tracts of simple repetitive DNA (poly[G1-3T]). Mutations in the gene TEL1 result in shortened telomeres. Sequence analysis of TEL1 indicates that it encodes a very large (322 kDa) protein with amino acid motifs found in phosphatidylinositol/protein kinases. The closest homolog to TEL1 is...


    pub_type: 杂志文章


    authors: Greenwell PW,Kronmal SL,Porter SE,Gassenhuber J,Obermaier B,Petes TD

    更新日期:1995-09-08 00:00:00

  • The evi5 oncogene regulates cyclin accumulation by stabilizing the anaphase-promoting complex inhibitor emi1.

    abstract::The anaphase-promoting complex/cyclosome (APC/C) inhibitor Emi1 controls progression to S phase and mitosis by stabilizing key APC/C ubiquitination substrates, including cyclin A. Examining Emi1 binding proteins, we identified the Evi5 oncogene as a regulator of Emi1 accumulation. Evi5 antagonizes SCF(betaTrCP)-depend...


    pub_type: 杂志文章


    authors: Eldridge AG,Loktev AV,Hansen DV,Verschuren EW,Reimann JD,Jackson PK

    更新日期:2006-01-27 00:00:00

  • Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes.

    abstract::Transcription hinders replication fork progression and stability. The ATR checkpoint and specialized DNA helicases assist DNA synthesis across transcription units to protect genome integrity. Combining genomic and genetic approaches together with the analysis of replication intermediates, we searched for factors coord...


    pub_type: 杂志文章


    authors: Alzu A,Bermejo R,Begnis M,Lucca C,Piccini D,Carotenuto W,Saponaro M,Brambati A,Cocito A,Foiani M,Liberi G

    更新日期:2012-11-09 00:00:00

  • An IL-23R/IL-22 Circuit Regulates Epithelial Serum Amyloid A to Promote Local Effector Th17 Responses.

    abstract::RORγt(+) Th17 cells are important for mucosal defenses but also contribute to autoimmune disease. They accumulate in the intestine in response to microbiota and produce IL-17 cytokines. Segmented filamentous bacteria (SFB) are Th17-inducing commensals that potentiate autoimmunity in mice. RORγt(+) T cells were induced...


    pub_type: 杂志文章


    authors: Sano T,Huang W,Hall JA,Yang Y,Chen A,Gavzy SJ,Lee JY,Ziel JW,Miraldi ER,Domingos AI,Bonneau R,Littman DR

    更新日期:2015-10-08 00:00:00

  • Injection of mitochondria into human cells leads to a rapid replacement of the endogenous mitochondrial DNA.

    abstract::Isolated human mitochondria containing a mitochondrial DNA (mtDNA) coded chloramphenicol resistance marker were injected into cells from two different human sensitive cell lines, 143BTK- and HT1080-6TG, which had been partially depleted of their mtDNA by ethidium bromide treatment. On the basis of the available eviden...


    pub_type: 杂志文章


    authors: King MP,Attardi G

    更新日期:1988-03-25 00:00:00

  • Conversion of Bcl-2 from protector to killer by interaction with nuclear orphan receptor Nur77/TR3.

    abstract::The Bcl-2 family proteins are key regulators of apoptosis in human diseases and cancers. Though known to block apoptosis, Bcl-2 promotes cell death through an undefined mechanism. Here, we show that Bcl-2 interacts with orphan nuclear receptor Nur77 (also known as TR3), which is required for cancer cell apoptosis indu...


    pub_type: 杂志文章


    authors: Lin B,Kolluri SK,Lin F,Liu W,Han YH,Cao X,Dawson MI,Reed JC,Zhang XK

    更新日期:2004-02-20 00:00:00

  • Human TFIID binds to core promoter DNA in a reorganized structural state.

    abstract::A mechanistic description of metazoan transcription is essential for understanding the molecular processes that govern cellular decisions. To provide structural insights into the DNA recognition step of transcription initiation, we used single-particle electron microscopy (EM) to visualize human TFIID with promoter DN...


    pub_type: 杂志文章


    authors: Cianfrocco MA,Kassavetis GA,Grob P,Fang J,Juven-Gershon T,Kadonaga JT,Nogales E

    更新日期:2013-01-17 00:00:00

  • Antisense RNA inhibits expression of membrane skeleton protein 4.1 during embryonic development of Xenopus.

    abstract::Plasmids expressing partial-length sense or antisense protein 4.1 RNA were microinjected into fertilized Xenopus eggs. Nuclease protection assays reveal that antisense protein 4.1 RNA lead to the specific loss of endogenous protein 4.1 transcripts after midblastula transition, with no effect on the levels of three unr...


    pub_type: 杂志文章


    authors: Giebelhaus DH,Eib DW,Moon RT

    更新日期:1988-05-20 00:00:00

  • Transcription of a gene for human U1 small nuclear RNA.

    abstract::Transcription of U1 small nuclear RNA from a 592 bp fragment of human DNA was analyzed in vivo and in vitro. When injected into Xenopus laevis oocyte nuclei, the cloned DNA is transcribed by RNA polymerase II to make human U1 snRNA. Thus the sequences of this fragment are sufficient for expression of the U1 snRNA gene...


    pub_type: 杂志文章


    authors: Murphy JT,Burgess RR,Dahlberg JE,Lund E

    更新日期:1982-05-01 00:00:00

  • Specific sequences and a hairpin structure in the template strand are required for N4 virion RNA polymerase promoter recognition.

    abstract::Coliphage N4 virion-encapsidated, DNA-dependent RNA polymerase (vRNAP) is inactive on double-stranded N4 DNA; however, denatured promoter-containing templates are accurately transcribed. We report that all determinants of vRNAP promoter recognition exist in the template strand, indicating that this enzyme is a site-sp...


    pub_type: 杂志文章


    authors: Glucksmann MA,Markiewicz P,Malone C,Rothman-Denes LB

    更新日期:1992-08-07 00:00:00

  • The unusual conformation adopted by the adenine tracts in kinetoplast DNA.

    abstract::To determine the structural features responsible for the curvature of kinetoplast DNA, we studied 13 adenine tracts in Crithidia fasciculata kinetoplast DNA. The structures of the A tracts were analyzed by cutting the DNA with hydroxyl radical. Reactivity of hydroxyl radical toward the DNA backbone progressively decre...


    pub_type: 杂志文章


    authors: Burkhoff AM,Tullius TD

    更新日期:1987-03-27 00:00:00

  • Loss of a gp130 cardiac muscle cell survival pathway is a critical event in the onset of heart failure during biomechanical stress.

    abstract::Biomechanical stress is a major stimulus for cardiac hypertrophy and the transition to heart failure. By generating mice that harbor a ventricular restricted knockout of the gp130 cytokine receptor via Cre-IoxP-mediated recombination, we demonstrate a critical role for a gp130-dependent myocyte survival pathway in the...


    pub_type: 杂志文章


    authors: Hirota H,Chen J,Betz UA,Rajewsky K,Gu Y,Ross J Jr,Müller W,Chien KR

    更新日期:1999-04-16 00:00:00

  • Evidence that Armadillo transduces wingless by mediating nuclear export or cytosolic activation of Pangolin.

    abstract::Secreted proteins of the Wnt family have profound organizing roles during animal development and are transduced via the activities of the Frizzled (Fz) class of transmembrane receptors and the TCF/LEF/Pangolin class of transcription factors. beta-catenins, including Drosophila Armadillo (Arm), link activation of Fz at...


    pub_type: 杂志文章,收录出版


    authors: Chan SK,Struhl G

    更新日期:2002-10-18 00:00:00

  • The secret life of ACE2 as a receptor for the SARS virus.

    abstract::The membrane-associated carboxypeptidase angiotensin-converting enzyme 2 (ACE2) is an essential regulator of heart function. Now, Li at al. identify and characterize an unexpected second function of ACE2 as a partner of the SARS-CoV spike glycoprotein in mediating virus entry and cell fusion. ...


    pub_type: 杂志文章


    authors: Dimitrov DS

    更新日期:2003-12-12 00:00:00