Abstract：
:Brown and beige adipocytes are specialized cells that express uncoupling protein 1 (UCP1) and dissipate chemical energy as heat. These cells likely possess alternative UCP1-independent thermogenic mechanisms. Here, we identify a secreted enzyme, peptidase M20 domain containing 1 (PM20D1), that is enriched in UCP1(+) versus UCP1(-) adipocytes. We demonstrate that PM20D1 is a bidirectional enzyme in vitro, catalyzing both the condensation of fatty acids and amino acids to generate N-acyl amino acids and also the reverse hydrolytic reaction. N-acyl amino acids directly bind mitochondria and function as endogenous uncouplers of UCP1-independent respiration. Mice with increased circulating PM20D1 have augmented respiration and increased N-acyl amino acids in blood. Lastly, administration of N-acyl amino acids to mice improves glucose homeostasis and increases energy expenditure. These data identify an enzymatic node and a family of metabolites that regulate energy homeostasis. This pathway might be useful for treating obesity and associated disorders.
journal_name
Celljournal_title
Cellauthors
Long JZ,Svensson KJ,Bateman LA,Lin H,Kamenecka T,Lokurkar IA,Lou J,Rao RR,Chang MR,Jedrychowski MP,Paulo JA,Gygi SP,Griffin PR,Nomura DK,Spiegelman BMdoi
10.1016/j.cell.2016.05.071subject
Has Abstractpub_date
2016-07-14 00:00:00pages
424-435issue
2eissn
0092-8674issn
1097-4172pii
S0092-8674(16)30675-4journal_volume
166pub_type
杂志文章相关文献
CELL文献大全abstract：:Fast-spiking interneurons (FSIs) are a prominent class of forebrain GABAergic cells implicated in two seemingly independent network functions: gain control and network plasticity. Little is known, however, about how these roles interact. Here, we use a combination of cell-type-specific ablation, optogenetics, electrop...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.01.005
更新日期：2018-02-08 00:00:00
abstract：:A functional cDNA from Aspergillus nidulans encoding triosephosphate isomerase (TPI) was isolated by its ability to complement a tpi1 mutation in Saccharomyces cerevisiae. This cDNA was used to obtain the corresponding gene, tpiA. Alignment of the cDNA and genomic DNA nucleotide sequences indicated that tpiA contains ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90868-8
更新日期：1986-07-04 00:00:00
abstract：:A "switch" from oxidative phosphorylation (OXPHOS) to aerobic glycolysis is a hallmark of T cell activation and is thought to be required to meet the metabolic demands of proliferation. However, why proliferating cells adopt this less efficient metabolism, especially in an oxygen-replete environment, remains incomplet...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.05.016
更新日期：2013-06-06 00:00:00
abstract：:Protein kinase C (PKC) isozymes have remained elusive cancer targets despite the unambiguous tumor promoting function of their potent ligands, phorbol esters, and the prevalence of their mutations. We analyzed 8% of PKC mutations identified in human cancers and found that, surprisingly, most were loss of function and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.01.001
更新日期：2015-01-29 00:00:00
abstract：:A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein. Southern blotting with PrP cDNA revealed a single gene with the same restriction patterns in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90333-2
更新日期：1985-04-01 00:00:00
abstract：:The lack of in vitro prostate cancer models that recapitulate the diversity of human prostate cancer has hampered progress in understanding disease pathogenesis and therapy response. Using a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specimens and circulating tumor cells....
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.08.016
更新日期：2014-09-25 00:00:00
abstract：:247 independent events involving insertion of the TN3 transposable element into a 4 kb constructed plasmid (pTU4) of partially known DNA sequence were studied by restriction endonuclease mapping, and 65 of these insertion sites were examined further by DNA sequence analysis. Our results show that the previously propos...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90396-7
更新日期：1980-01-01 00:00:00
abstract：:Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan ribos...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.06.051
更新日期：2007-09-07 00:00:00
abstract：:gamma-Irradiation of human diploid fibroblasts in the G1 interval caused arrest of the cell cycle prior to S phase. This cell cycle block was correlated with a lack of activation of both cyclin E-Cyclin-dependent kinase 2 (Cdk2) and cyclin A-Cdk2 kinases and depended on wild-type p53. Although the accumulation of cycl...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90379-4
更新日期：1994-03-25 00:00:00
abstract：:There is tremendous enthusiasm for the microbiome in academia and industry. This Perspective argues that in order to realize its potential, the field needs to focus on establishing causation and molecular mechanism with an emphasis on phenotypes that are large in magnitude, easy to measure, and unambiguously driven by...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2018.07.038
更新日期：2018-08-09 00:00:00
abstract：:The rapid cell proliferation characteristic of early animal embryos is accomplished with an abbreviated cell cycle and no DNA replication checkpoint. Blythe and Wieschaus provide evidence that nascent zygotic transcription precedes—and may trigger—this checkpoint at the midblastula transition. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2015.02.051
更新日期：2015-03-12 00:00:00
abstract：:The sequences corresponding to the 18S and 28S rRNAs have been mapped within a cloned 17 kilobase (kb) fragment formed by Eco R1 cleavage of Drosophila melanogaster rDNA. This fragment, Dm103, represents the longer of two major types of repeating units that are present in the rDNA of this fly, and was cloned as a hybr...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(77)90212-4
更新日期：1977-02-01 00:00:00
abstract：:3.18 kb fragments of X. laevis DNA coding for tRNA 1 met have been inserted into a lambda vector via Hind III termini and cloned in E. coli. The organization of one cloned fragment has been analyzed by restriction endonuclease digestion and RNA-DNA hybridization. From the distribution of sites for three enzymes, this ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90253-2
更新日期：1978-07-01 00:00:00
abstract：:The full-length cDNA for a transcriptional activator, DBP, that binds to the D site of the albumin promoter has been cloned. DBP belongs to a family of related transcription factors including Fos, Jun, CREB, and C/EBP, which share a conserved basic domain. However, unlike most other members of this family, DBP does no...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90808-r
更新日期：1990-04-20 00:00:00
abstract：:Protease-activated receptors (PARs) are a unique class of G protein-coupled receptors that play critical roles in thrombosis, inflammation, and vascular biology. PAR1 is proposed to be involved in the invasive and metastatic processes of various cancers. However, the protease responsible for activating the proinvasive...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.12.018
更新日期：2005-02-11 00:00:00
abstract：:In this issue, Stetson et al. (2008) report a mechanism by which host cells avert an autoimmune response to self-nucleic acids. They show that the nuclease Trex1 prevents the accumulation of DNA derived from endogenous retroelements that, if left unchecked, trigger elevated production of type I interferons leading to ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2008.08.010
更新日期：2008-08-22 00:00:00
abstract：:The V(D)J recombinase subunits Rag-1 and Rag-2 mediate assembly of antigen receptor gene segments. We studied the mechanisms of DNA recognition by Rag-1/Rag-2 using surface plasmon resonance. The critical step for signal recognition is binding of Rag-1 to the nonamer. This is achieved by a region of Rag-1 homologous t...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81344-6
更新日期：1996-10-18 00:00:00
abstract：:sigma(70) subunit is thought to be released from the core RNA polymerase (RNAP) upon the transition from initiation to elongation or shortly afterward. Here, we identify a population of RNAP from E. coli that retains sigma(70) throughout elongation. The relative amount of this population appears to depend on cellular ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00461-5
更新日期：2001-08-24 00:00:00
abstract：:Rosalind Franklin provided the key data for deriving the double helix structure of DNA. The English chemist also pioneered structural studies of colloids, viruses, and RNA. To celebrate the 100th anniversary of Franklin's birth, I summarize her work, which shaped the emerging discipline of molecular biology. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.07.028
更新日期：2020-08-20 00:00:00
abstract：:A gel electrophoresis DNA binding assay has been used to identify proteins in HeLa cell extracts that specifically bind to the major late promoter of adenovirus. A major late promoter transcription factor MLTF has been detected as a discrete protein-DNA complex. MLTF binds specifically and with high affinity to sequen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90174-6
更新日期：1985-12-01 00:00:00
abstract：:The high affinity receptor that binds the Fc domain of immunoglobulin G (IgG) subclasses 1 and 3 (Fc gamma RI) mediates important immune defense functions by inducing cell surface changes on human leukocytes. In this article, we document direct high affinity binding of Fc gamma RI to the actin filament cross-linking p...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90179-3
更新日期：1991-10-18 00:00:00
abstract：:Plants, compared to animals, exhibit an amazing adaptability and plasticity in their development. This is largely dependent on the ability of plants to form new organs, such as lateral roots, leaves, and flowers during postembryonic development. Organ primordia develop from founder cell populations into organs by coor...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(03)00924-3
更新日期：2003-11-26 00:00:00
abstract：:Cadherins are a group of homophilic intercellular adhesion molecules; each member of this family exhibits binding specificity. Here, we attempted to map the sites for the specificities of these molecules by analyzing adhesives selectivities of the cells that express chimeric and point-mutated E- and P-cadherin. The re...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90222-z
更新日期：1990-04-06 00:00:00
abstract：:Chronic viral infections represent a major burden to human health, and modulation of the immune system is emerging as a novel approach to fighting such infections. Pellegrini et al. (2011) demonstrate that treatment with the cytokine IL-7 may reinvigorate the immune response to persistent infection by targeting immuno...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2011.01.038
更新日期：2011-02-18 00:00:00
abstract：:Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova tel...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81720-1
更新日期：1998-12-23 00:00:00
abstract：:CD4 T cells potentiate the inflammatory or humoral immune response through the action of Th1 and Th2 cells, respectively. The molecular basis of the differentiation of these cells from naive T cell precursors is, however, unclear. We found that GATA-3 was selectively expressed in Th2 cells. GATA-3 is expressed at a hi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80240-8
更新日期：1997-05-16 00:00:00
abstract：:Establishment and maintenance of the blood system relies on self-renewing hematopoietic stem cells (HSCs) that normally reside in small numbers in the bone marrow niche of adult mammals. This Review describes the developmental origins of HSCs and the molecular mechanisms that regulate lineage-specific differentiation....
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.01.025
更新日期：2008-02-22 00:00:00
abstract：:Much like the "microbe hypothesis" put forth over 150 years ago, the "prion hypothesis" can be definitely proven only if a prion disease is engendered in a natural host from an infectious prion produced in vitro. In this issue of Cell, come very close to accomplishing this goal by producing a prion disease in a natura...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2005.04.002
更新日期：2005-04-22 00:00:00
abstract：:N(6)-methyladenosine (m(6)A) is the most abundant internal modification of messenger RNA. While the presence of m(6)A on transcripts can impact nuclear RNA fates, a reader of this mark that mediates processing of nuclear transcripts has not been identified. We find that the RNA-binding protein HNRNPA2B1 binds m(6)A-be...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.08.011
更新日期：2015-09-10 00:00:00
abstract：:The membrane-bound form of immunoglobulin serves as an antigen-specific receptor for B cells mediating signal transduction and antigen presentation. We have developed an assay that reconstitutes both these physiologic responses with respect to the antigen phosphorylcholine. By introducing specific mutations in the hum...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90171-a
更新日期：1990-10-19 00:00:00
