Structure and mechanism of a metal-sensing regulatory RNA.


:Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan riboswitch, renamed the "M-box," is a divalent metal-sensing RNA involved in Mg(2+) homeostasis. A combination of genetic, biochemical, and biophysical techniques demonstrate that Mg(2+) induces a compacted tertiary architecture for M-box RNAs that regulates the accessibility of nucleotides involved in genetic control. Molecular details are provided by crystallographic structure determination of a Mg(2+)-bound M-box RNA. Given the distribution of this RNA element, it may constitute a common mode for bacterial metal ion regulation, and its discovery suggests the possibility of additional RNA-based metal sensors in modern and primordial organisms.






Dann CE 3rd,Wakeman CA,Sieling CL,Baker SC,Irnov I,Winkler WC




Has Abstract


2007-09-07 00:00:00
















  • Whence meiosis?

    abstract::Sexual reproduction predominates among eukaryotic organisms on our planet. While debate continues over why this should be so, burgeoning genomic and functional information now allows us to begin to think reasonably about some of the events that may have occurred to make sex possible in the first place. ...


    pub_type: 杂志文章,评审


    authors: Villeneuve AM,Hillers KJ

    更新日期:2001-09-21 00:00:00

  • Targeted disruption of the Huntington's disease gene results in embryonic lethality and behavioral and morphological changes in heterozygotes.

    abstract::Huntington's disease (HD) is an incurable neuropsychiatric disease associated with CAG repeat expansion within a widely expressed gene that causes selective neuronal death. To understand its normal function, we have created a targeted disruption in exon 5 of Hdh (Hdhex5), the murine homolog of the HD gene. Homozygotes...


    pub_type: 杂志文章


    authors: Nasir J,Floresco SB,O'Kusky JR,Diewert VM,Richman JM,Zeisler J,Borowski A,Marth JD,Phillips AG,Hayden MR

    更新日期:1995-06-02 00:00:00

  • Recovery of S. cerevisiae a cells from G1 arrest by alpha factor pheromone requires endopeptidase action.

    abstract::Radioactive alpha factor is degraded to discrete biologically inactive fragments by the target a cells of S. cerevisiae, but not by alpha cells which make the pheromone. The pattern of cleavage products and sequence analysis of one fragment indicated that the first scission occurred between leucine 6 and lysine 7. The...


    pub_type: 杂志文章


    authors: Ciejek E,Thorner J

    更新日期:1979-11-01 00:00:00

  • p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells.

    abstract::Characteristic of chronic myelogenous leukemia (CML) is the presence of the chimeric p210(bcr-abl) protein possessing elevated protein tyrosine kinase activity relative to normal c-abl tyrosine kinase. Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphory...


    pub_type: 杂志文章


    authors: Carpino N,Wisniewski D,Strife A,Marshak D,Kobayashi R,Stillman B,Clarkson B

    更新日期:1997-01-24 00:00:00

  • Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity.

    abstract::Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequen...


    pub_type: 杂志文章


    authors: Volz E,Hill V,McCrone JT,Price A,Jorgensen D,O'Toole Á,Southgate J,Johnson R,Jackson B,Nascimento FF,Rey SM,Nicholls SM,Colquhoun RM,da Silva Filipe A,Shepherd J,Pascall DJ,Shah R,Jesudason N,Li K,Jarrett R,Pacchi

    更新日期:2021-01-07 00:00:00

  • Switch between large hand-over-hand and small inchworm-like steps in myosin VI.

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    pub_type: 杂志文章


    authors: Nishikawa S,Arimoto I,Ikezaki K,Sugawa M,Ueno H,Komori T,Iwane AH,Yanagida T

    更新日期:2010-09-17 00:00:00

  • Drosophila p53 binds a damage response element at the reaper locus.

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    pub_type: 杂志文章


    authors: Brodsky MH,Nordstrom W,Tsang G,Kwan E,Rubin GM,Abrams JM

    更新日期:2000-03-31 00:00:00

  • Cellular motility driven by assembly and disassembly of actin filaments.

    abstract::Motile cells extend a leading edge by assembling a branched network of actin filaments that produces physical force as the polymers grow beneath the plasma membrane. A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematica...


    pub_type: 杂志文章,评审


    authors: Pollard TD,Borisy GG

    更新日期:2003-02-21 00:00:00

  • Functional analysis of a growth factor-responsive transcription factor complex.

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    pub_type: 杂志文章


    authors: Hill CS,Marais R,John S,Wynne J,Dalton S,Treisman R

    更新日期:1993-04-23 00:00:00

  • Premature activation of the SLX4 complex by Vpr promotes G2/M arrest and escape from innate immune sensing.

    abstract::The HIV auxiliary protein Vpr potently blocks the cell cycle at the G2/M transition. Here, we show that G2/M arrest results from untimely activation of the structure-specific endonuclease (SSE) regulator SLX4 complex (SLX4com) by Vpr, a process that requires VPRBP-DDB1-CUL4 E3-ligase complex. Direct interaction of Vpr...


    pub_type: 杂志文章


    authors: Laguette N,Brégnard C,Hue P,Basbous J,Yatim A,Larroque M,Kirchhoff F,Constantinou A,Sobhian B,Benkirane M

    更新日期:2014-01-16 00:00:00

  • Recognition of polyadenylate RNA by the poly(A)-binding protein.

    abstract::The cocrystal structure of human poly(A)-binding protein (PABP) has been determined at 2.6 A resolution. PABP recognizes the 3' mRNA poly(A) tail and plays critical roles in eukaryotic translation initiation and mRNA stabilization/degradation. The minimal PABP used in this study consists of the N-terminal two RRM-type...


    pub_type: 杂志文章


    authors: Deo RC,Bonanno JB,Sonenberg N,Burley SK

    更新日期:1999-09-17 00:00:00

  • An extensively edited mitochondrial transcript in kinetoplastids encodes a protein homologous to ATPase subunit 6.

    abstract::The mitochondrial MURF4 gene of T. brucei has pronounced G versus C strand bias and heterogeneously sized transcripts, characteristic of genes encoding extensively edited transcripts. We find that MURF4 transcripts of T. brucei are extensively edited throughout by the addition and deletion of numerous uridines, creati...


    pub_type: 杂志文章


    authors: Bhat GJ,Koslowsky DJ,Feagin JE,Smiley BL,Stuart K

    更新日期:1990-06-01 00:00:00

  • Protein degradation in E. coli: the lon mutation and bacteriophage lambda N and cII protein stability.

    abstract::The Ion gene of E. coli controls the stability of two bacteriophage lambda proteins. The functional half-life of the phage N gene product, measured by complementation, is increased about 5-fold in Ion mutant strains, from 2 min to 10 min. The chemical half-life of N protein, determined by its disappearance on polyacry...


    pub_type: 杂志文章


    authors: Gottesman S,Gottesman M,Shaw JE,Pearson ML

    更新日期:1981-04-01 00:00:00

  • Universal Chimeric Antigen Receptors for Multiplexed and Logical Control of T Cell Responses.

    abstract::T cells expressing chimeric antigen receptors (CARs) are promising cancer therapeutic agents, with the prospect of becoming the ultimate smart cancer therapeutics. To expand the capability of CAR T cells, here, we present a split, universal, and programmable (SUPRA) CAR system that simultaneously encompasses multiple ...


    pub_type: 杂志文章


    authors: Cho JH,Collins JJ,Wong WW

    更新日期:2018-05-31 00:00:00

  • Divided within: heterogeneity within adult stem cell pools.

    abstract::In this issue, Wilson et al. (2008) demonstrate that there are two functional subsets of hematopoietic stem cells that have distinctive kinetics of cell cycling. They present evidence that cells may transition between the two kinetic states, establishing one subpopulation that is ready to proliferate and another that ...


    pub_type: 评论,杂志文章


    authors: Raaijmakers MH,Scadden DT

    更新日期:2008-12-12 00:00:00

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    pub_type: 杂志文章


    authors: Zhu S,Lin S,Kao CF,Awasaki T,Chiang AS,Lee T

    更新日期:2006-10-20 00:00:00

  • Breast Milk Modulates Transgenerational Immune Inheritance.

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    pub_type: 评论,杂志文章


    authors: Zimmermann J,Macpherson AJ

    更新日期:2020-06-11 00:00:00

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    pub_type: 评论,杂志文章,评审


    authors: Turner BM

    更新日期:2002-11-01 00:00:00

  • The canonical Notch signaling pathway: unfolding the activation mechanism.

    abstract::Notch signaling regulates many aspects of metazoan development and tissue renewal. Accordingly, the misregulation or loss of Notch signaling underlies a wide range of human disorders, from developmental syndromes to adult-onset diseases and cancer. Notch signaling is remarkably robust in most tissues even though each ...


    pub_type: 杂志文章,评审


    authors: Kopan R,Ilagan MX

    更新日期:2009-04-17 00:00:00

  • Protein kinase C phosphorylates pp60src at a novel site.

    abstract::The transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homolog (pp60c-src) are demonstrated to be phosphorylated at serine 12 in vivo under certain conditions. We propose that protein kinase C is responsible for this modification based on the following evidence. First, the tumor promoters, ...


    pub_type: 杂志文章


    authors: Gould KL,Woodgett JR,Cooper JA,Buss JE,Shalloway D,Hunter T

    更新日期:1985-10-01 00:00:00

  • Structural insight into partner specificity and phosphoryl transfer in two-component signal transduction.

    abstract::The chief mechanism used by bacteria for sensing their environment is based on two conserved proteins: a sensor histidine kinase (HK) and an effector response regulator (RR). The signal transduction process involves highly conserved domains of both proteins that mediate autokinase, phosphotransfer, and phosphatase act...


    pub_type: 杂志文章


    authors: Casino P,Rubio V,Marina A

    更新日期:2009-10-16 00:00:00

  • MIX-1: an essential component of the C. elegans mitotic machinery executes X chromosome dosage compensation.

    abstract::We show that a functional component of the C. elegans mitotic machinery regulates X chromosome gene expression. This protein, MIX-1, is a member of the dosage compensation complex that associates specifically with hermaphrodite X chromosomes to reduce their gene expression during interphase. MIX-1 also associates with...


    pub_type: 杂志文章


    authors: Lieb JD,Albrecht MR,Chuang PT,Meyer BJ

    更新日期:1998-01-23 00:00:00

  • Cooperativity in transactivation between retinoic acid receptor and TFIID requires an activity analogous to E1A.

    abstract::In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally coope...


    pub_type: 杂志文章


    authors: Berkenstam A,Vivanco Ruiz MM,Barettino D,Horikoshi M,Stunnenberg HG

    更新日期:1992-05-01 00:00:00

  • Parafibromin/Hyrax activates Wnt/Wg target gene transcription by direct association with beta-catenin/Armadillo.

    abstract::The Wnt pathway controls cell fates, tissue homeostasis, and cancer. Its activation entails the association of beta-catenin with nuclear TCF/LEF proteins and results in transcriptional activation of target genes. The mechanism by which nuclear beta-catenin controls transcription is largely unknown. Here we genetically...


    pub_type: 杂志文章


    authors: Mosimann C,Hausmann G,Basler K

    更新日期:2006-04-21 00:00:00

  • Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells.

    abstract::The COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here, we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput single-cell RNA and VDJ sequencing of antigen-enriched B cells from 60 convalescent patients. From 8,558 antigen-binding IgG1+ clonotypes, 1...


    pub_type: 杂志文章


    authors: Cao Y,Su B,Guo X,Sun W,Deng Y,Bao L,Zhu Q,Zhang X,Zheng Y,Geng C,Chai X,He R,Li X,Lv Q,Zhu H,Deng W,Xu Y,Wang Y,Qiao L,Tan Y,Song L,Wang G,Du X,Gao N,Liu J,Xiao J,Su XD,Du Z,Feng Y,Qin C,Qin C

    更新日期:2020-07-09 00:00:00

  • PKCε promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria.

    abstract::The transcription factor ATF2 elicits oncogenic activities in melanoma and tumor suppressor activities in nonmalignant skin cancer. Here, we identify that ATF2 tumor suppressor function is determined by its ability to localize at the mitochondria, where it alters membrane permeability following genotoxic stress. The a...


    pub_type: 杂志文章


    authors: Lau E,Kluger H,Varsano T,Lee K,Scheffler I,Rimm DL,Ideker T,Ronai ZA

    更新日期:2012-02-03 00:00:00

  • Genetic recombination of homologous plasmids catalyzed by cell-free extracts of Saccharomyces cerevisiae.

    abstract::We have developed an in vitro system utilizing yeast cell-free extracts to catalyze recombination events between homologous plasmids containing different mutant alleles of the Tet or ARG4 genes. The reaction increased the frequency of Tcr or Arg+ transformants (recombinants) from 2 X 10(-6) to 1-3 X 10(-3). Linearizin...


    pub_type: 杂志文章


    authors: Symington LS,Fogarty LM,Kolodner R

    更新日期:1983-12-01 00:00:00

  • Structural basis for activation of ARF GTPase: mechanisms of guanine nucleotide exchange and GTP-myristoyl switching.

    abstract::Ras-related GTPases are positively regulated by guanine nucleotide exchange factors (GEFs) that promote the exchange of GDP for GTP. The crystal structure of the Sec7 domain GEF bound to nucleotide-free ARF1 GTPase has been determined at 2.8 A resolution and the structure of ARF1 in the GTP-analog form determined at 1...


    pub_type: 杂志文章


    authors: Goldberg J

    更新日期:1998-10-16 00:00:00

  • Molecular basis of myotonic dystrophy: expansion of a trinucleotide (CTG) repeat at the 3' end of a transcript encoding a protein kinase family member.

    abstract::Using positional cloning strategies, we have identified a CTG triplet repeat that undergoes expansion in myotonic dystrophy patients. This sequence is highly variable in the normal population. PCR analysis of the interval containing this repeat indicates that unaffected individuals have been 5 and 27 copies. Myotonic ...


    pub_type: 杂志文章


    authors: Brook JD,McCurrach ME,Harley HG,Buckler AJ,Church D,Aburatani H,Hunter K,Stanton VP,Thirion JP,Hudson T

    更新日期:1992-02-21 00:00:00

  • Insulin signaling: inositol phosphates get into the Akt.

    abstract::An acute but transient response to insulin is essential for glucose homeostasis in mammals. Chakraborty et al. (2010) uncover a new feedback mechanism regulating insulin signaling. They show that the inositol pyrophosphate IP7, which is produced in response to insulin, inhibits the Akt kinase, a primary effector of in...


    pub_type: 评论,杂志文章


    authors: Manning BD

    更新日期:2010-12-10 00:00:00