Structure and mechanism of a metal-sensing regulatory RNA.

Abstract:

:Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan riboswitch, renamed the "M-box," is a divalent metal-sensing RNA involved in Mg(2+) homeostasis. A combination of genetic, biochemical, and biophysical techniques demonstrate that Mg(2+) induces a compacted tertiary architecture for M-box RNAs that regulates the accessibility of nucleotides involved in genetic control. Molecular details are provided by crystallographic structure determination of a Mg(2+)-bound M-box RNA. Given the distribution of this RNA element, it may constitute a common mode for bacterial metal ion regulation, and its discovery suggests the possibility of additional RNA-based metal sensors in modern and primordial organisms.

journal_name

Cell

journal_title

Cell

authors

Dann CE 3rd,Wakeman CA,Sieling CL,Baker SC,Irnov I,Winkler WC

doi

10.1016/j.cell.2007.06.051

subject

Has Abstract

pub_date

2007-09-07 00:00:00

pages

878-92

issue

5

eissn

0092-8674

issn

1097-4172

pii

S0092-8674(07)00901-4

journal_volume

130

pub_type

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