Abstract：
:Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan riboswitch, renamed the "M-box," is a divalent metal-sensing RNA involved in Mg(2+) homeostasis. A combination of genetic, biochemical, and biophysical techniques demonstrate that Mg(2+) induces a compacted tertiary architecture for M-box RNAs that regulates the accessibility of nucleotides involved in genetic control. Molecular details are provided by crystallographic structure determination of a Mg(2+)-bound M-box RNA. Given the distribution of this RNA element, it may constitute a common mode for bacterial metal ion regulation, and its discovery suggests the possibility of additional RNA-based metal sensors in modern and primordial organisms.
journal_name
Celljournal_title
Cellauthors
Dann CE 3rd,Wakeman CA,Sieling CL,Baker SC,Irnov I,Winkler WCdoi
10.1016/j.cell.2007.06.051subject
Has Abstractpub_date
2007-09-07 00:00:00pages
878-92issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(07)00901-4journal_volume
130pub_type
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