Abstract:
:I have identified an activity in crude HeLa cell extracts that satisfies the requirements for a human telomere terminal transferase or telomerase. It catalyzes the addition of a 6 nucleotide repeating pattern to oligonucleotide primers containing human or nonhuman telomeric repeat sequences. Direct sequence analyses of reaction products reveal the added sequence to be TTAGGG in all cases. Under optimal conditions 65-70 repeats can be synthesized. The enzyme has the properties of a ribonucleoprotein. Telomerase has previously been observed only in ciliated protozoans, which possess 10(4) - 10(7) macronuclear telomeres. The identification of telomerase in HeLa cells with only approximately 100 telomeres indicates that telomerase-mediated telomere maintenance is conserved throughout eukaryotes.
journal_name
Celljournal_title
Cellauthors
Morin GBdoi
10.1016/0092-8674(89)90035-4subject
Has Abstractpub_date
1989-11-03 00:00:00pages
521-9issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(89)90035-4journal_volume
59pub_type
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