RNA polymerase interactions with the upstream region of the E. coli tyrT promoter.

Abstract:

:The rate of in vivo transcription from the E. coli tRNA and rRNA promoters depends on both cellular growth rate and aminoacid availability. To investigate the molecular mechanisms involved we determined the extent of interaction of RNA polymerase with the promoter of the tyrT stable RNA gene. We show that the enzyme can protect from DNAase I digestion a region of at least 85 bp of the wild-type tyrT promoter and only approximately 62 bp of the lacUV5 mRNA promoter, the protected region extending on the antisense strand to approximately 65 and 42 bp respectively upstream of the transcription startpoint. A mutant tyrT promoter, tyrTp27, is protected more extensively, RNA polymerase interactions extending to at least approximately -130. We propose that these upstream interactions of RNA polymerase perform two functions; activating initiation by polymerase bound at the primary binding site and increasing the concentration of polymerase in the vicinity of the tyrT promoter, thus allowing a high rate of maximal expression and enabling the promoter to be regulated over a wide range of activity.

journal_name

Cell

journal_title

Cell

authors

Travers AA,Lamond AI,Mace HA,Berman ML

doi

10.1016/0092-8674(83)90229-5

subject

Has Abstract

pub_date

1983-11-01 00:00:00

pages

265-73

issue

1

eissn

0092-8674

issn

1097-4172

pii

0092-8674(83)90229-5

journal_volume

35

pub_type

杂志文章

相关文献

CELL文献大全
  • Common Features of Enveloped Viruses and Implications for Immunogen Design for Next-Generation Vaccines.

    abstract::Enveloped viruses enter cells by inducing fusion of viral and cellular membranes, a process catalyzed by a specialized membrane-fusion protein expressed on their surface. This review focuses on recent structural studies of viral fusion proteins with an emphasis on their metastable prefusion form and on interactions wi...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.cell.2018.02.054

    authors: Rey FA,Lok SM

    更新日期:2018-03-08 00:00:00

  • Methyl labeling of HeLa cell hnRNA: a comparison with mRNA.

    abstract::The majority of the mRNA molecules in HeLa cells contain 1-2 residue(s) of m6Ap and one blocked, methylated 5' terminal "cap" structure. The hnRNA, which is longer than mRNA, contains both m6Ap and caps but 4-6 times as many m6Ap residues per chain. In addition, nuclear molecules contain T2 RNA ase-resistant, methyl-l...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(76)90022-2

    authors: Salditt-Georgieff M,Jelinek W,Darnell JE,Furuichi Y,Morgan M,Shatkin A

    更新日期:1976-02-01 00:00:00

  • Genome-wide mapping and characterization of Notch-regulated long noncoding RNAs in acute leukemia.

    abstract::Notch signaling is a key developmental pathway that is subject to frequent genetic and epigenetic perturbations in many different human tumors. Here we investigate whether long noncoding RNA (lncRNA) genes, in addition to mRNAs, are key downstream targets of oncogenic Notch1 in human T cell acute lymphoblastic leukemi...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2014.05.049

    authors: Trimarchi T,Bilal E,Ntziachristos P,Fabbri G,Dalla-Favera R,Tsirigos A,Aifantis I

    更新日期:2014-07-31 00:00:00

  • Going Public in Support of Science.

    abstract::Scientists are stepping up like never before to support science in the public arena. In big and small ways, scientists are adopting creative ideas to promote science. ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2017.03.029

    authors: Matheson S

    更新日期:2017-04-06 00:00:00

  • In vitro polyoma DNA synthesis: self-annealing properties of short DNA chains.

    abstract::Short DNA chains, isolated from in vitro pulse-labeled replicating polyoma DNA, exhibit some degree of self-complementarity (28% resistance to S1 nuclease after self-annealing to plateau levels). This level of self-annealing is not increased if short DNA chains present as free single-stranded DNA after extraction are ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(75)90028-8

    authors: Francke B,Vogt M

    更新日期:1975-06-01 00:00:00

  • The herpes simplex virus amplicon: a new eucaryotic defective-virus cloning-amplifying vector.

    abstract::We have employed repeat units of herpes simplex virus (HSV) defective genomes to derive a cloning-amplifying vector (amplicon) that can replicate in eucaryotic cells in the presence of standard HSV helper virus. The design of the HSV amplicon system is based on the previous observation that cotransfection of cells wit...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(82)90035-6

    authors: Spaete RR,Frenkel N

    更新日期:1982-08-01 00:00:00

  • Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.

    abstract::A plasmid containing the major late promoter was assembled into nucleosomes in a Xenopus oocyte extract, isolated by gel filtration, and found to be refractory to transcription initiation in vitro. However, exposure of the promoter to HeLa nuclear extract or to a mixture of isolated transcription factors prior to nucl...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(87)90130-9

    authors: Workman JL,Roeder RG

    更新日期:1987-11-20 00:00:00

  • Recognition of polyadenylate RNA by the poly(A)-binding protein.

    abstract::The cocrystal structure of human poly(A)-binding protein (PABP) has been determined at 2.6 A resolution. PABP recognizes the 3' mRNA poly(A) tail and plays critical roles in eukaryotic translation initiation and mRNA stabilization/degradation. The minimal PABP used in this study consists of the N-terminal two RRM-type...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)81517-2

    authors: Deo RC,Bonanno JB,Sonenberg N,Burley SK

    更新日期:1999-09-17 00:00:00

  • Nucleotide sequence of the triosephosphate isomerase gene from Aspergillus nidulans: implications for a differential loss of introns.

    abstract::A functional cDNA from Aspergillus nidulans encoding triosephosphate isomerase (TPI) was isolated by its ability to complement a tpi1 mutation in Saccharomyces cerevisiae. This cDNA was used to obtain the corresponding gene, tpiA. Alignment of the cDNA and genomic DNA nucleotide sequences indicated that tpiA contains ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(86)90868-8

    authors: McKnight GL,O'Hara PJ,Parker ML

    更新日期:1986-07-04 00:00:00

  • An active tissue-specific enhancer and bound transcription factors existing in a precisely positioned nucleosomal array.

    abstract::Nucleosomes positioned over promoters are usually inhibitory to protein binding and activity. We analyzed at the nucleotide level of resolution the nucleosomal organization of a distal, liver-specific enhancer in various mouse tissues and found that the enhancer exists in an array of three precisely positioned nucleos...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(93)80079-t

    authors: McPherson CE,Shim EY,Friedman DS,Zaret KS

    更新日期:1993-10-22 00:00:00

  • Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p.

    abstract::We have reproduced the posttranslational mode of protein translocation across the endoplasmic reticulum membrane with reconstituted proteoliposomes containing a purified complex of seven yeast proteins. This Sec complex includes a heterotrimeric Sec61p complex, homologous to that in mammals, as well as all other membr...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(95)90077-2

    authors: Panzner S,Dreier L,Hartmann E,Kostka S,Rapoport TA

    更新日期:1995-05-19 00:00:00

  • Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.

    abstract::In this report, we describe the isolation, sequence, and initial characterization of the cDNA for the muscle-specific regulatory factor skeletal myogenin. Transfection of myogenin into the mesenchymal cell line C3H10T1/2 produces cells expressing muscle-specific markers. Myogenin is absent in undifferentiated cells, p...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(89)90583-7

    authors: Wright WE,Sassoon DA,Lin VK

    更新日期:1989-02-24 00:00:00

  • Inherited human Caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune lymphoproliferative syndrome type II.

    abstract::Caspases are cysteine proteases that mediate programmed cell death in phylogenetically diverse multicellular organisms. We report here two kindreds with autoimmune lymphoproliferative syndrome (ALPS) type II, characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects, that harbor...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/S0092-8674(00)80605-4

    authors: Wang J,Zheng L,Lobito A,Chan FK,Dale J,Sneller M,Yao X,Puck JM,Straus SE,Lenardo MJ

    更新日期:1999-07-09 00:00:00

  • Nucleosome arrangement on tRNA genes of Xenopus laevis.

    abstract::We have examined the chromatin structure of a repeated cluster of tRNA genes of Xenopus laevis, with particular attention to possible phase relationships between nucleosomes and the underlying tDNA sequence. In transcriptionally active liver and cultured kidney cells, essentially all tDNA is packaged into regularly sp...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(81)90387-1

    authors: Bryan PN,Hofstetter H,Birnstiel ML

    更新日期:1981-12-01 00:00:00

  • Dynein motion switches from diffusive to directed upon cortical anchoring.

    abstract::Cytoplasmic dynein is a motor protein that exerts force on microtubules. To generate force for the movement of large organelles, dynein needs to be anchored, with the anchoring sites being typically located at the cell cortex. However, the mechanism by which dyneins target sites where they can generate large collectiv...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2013.05.020

    authors: Ananthanarayanan V,Schattat M,Vogel SK,Krull A,Pavin N,Tolić-Nørrelykke IM

    更新日期:2013-06-20 00:00:00

  • Single-Molecule Visualization of MCM2-7 DNA Loading: Seeing Is Believing.

    abstract::The first event in the initiation of eukaryotic DNA replication is the recruitment of the MCM2-7 ATPase, the core of the replicative DNA helicase, to origins. Ticau et al. use single-molecule imaging to reveal how ORC, Cdc6, and Cdt1 cooperate to load MCM2-7 onto DNA, enabling bidirectional replication. ...

    journal_title:Cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.cell.2015.04.006

    authors: Chistol G,Walter JC

    更新日期:2015-04-23 00:00:00

  • Crystallographic analysis of anti-p24 (HIV-1) monoclonal antibody cross-reactivity and polyspecificity.

    abstract::The X-ray crystal structures of an anti-p24 (HIV-1) monoclonal antibody Fab fragment alone and in complexes with the epitope peptide GATPQDLNTnL (n = norleucine), an epitope-homologous peptide GATPEDLNQKLAGN, as well as two unrelated peptides GLYEWGGARITNTD and efslkGpllqwrsG (D-peptide), are presented to a maximum re...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80469-9

    authors: Keitel T,Kramer A,Wessner H,Scholz C,Schneider-Mergener J,Höhne W

    更新日期:1997-12-12 00:00:00

  • A role for casein kinase 2 in the mechanism underlying circadian temperature compensation.

    abstract::Temperature compensation of circadian clocks is an unsolved problem with relevance to the general phenomenon of biological compensation. We identify casein kinase 2 (CK2) as a key regulator of temperature compensation of the Neurospora clock by determining that two long-standing clock mutants, chrono and period-3, dis...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2009.03.019

    authors: Mehra A,Shi M,Baker CL,Colot HV,Loros JJ,Dunlap JC

    更新日期:2009-05-15 00:00:00

  • Regulatory Expansion in Mammals of Multivalent hnRNP Assemblies that Globally Control Alternative Splicing.

    abstract::Alternative splicing (AS) patterns have diverged rapidly during vertebrate evolution, yet the functions of most species- and lineage-specific splicing events are not known. We observe that mammalian-specific AS events are enriched in transcript sequences encoding intrinsically disordered regions (IDRs) of proteins, in...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2017.06.037

    authors: Gueroussov S,Weatheritt RJ,O'Hanlon D,Lin ZY,Narula A,Gingras AC,Blencowe BJ

    更新日期:2017-07-13 00:00:00

  • A prion-like trigger of antiviral signaling.

    abstract::The MAVS protein plays a critical role in the assembly of an antiviral signaling complex on mitochondrial membranes. Hou et al. (2011) now report that virus infection induces a conformational change in MAVS, leading to the prion-like formation of functional self-aggregates that provide a sensitive trigger for antivira...

    journal_title:Cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.cell.2011.07.018

    authors: Ye J,Maniatis T

    更新日期:2011-08-05 00:00:00

  • Secondary structure of mouse and rabbit alpha- and beta-globin mRNAs: differential accessibility of alpha and beta initiator AUG codons towards nucleases.

    abstract::The nucleotide sequence from the 5' terminus inward of one third of mouse alpha- and beta maj-globin messenger RNAs has been established. In addition, using 5' 32P end-labeled mRNAs as substrates and S1 and T1 nucleases as probes for single-stranded regions, the secondary structures of mouse and rabbit alpha- and beta...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(80)90391-8

    authors: Pavlakis GN,Lockard RE,Vamvakopoulos N,Rieser L,RajBhandary UL,Vournakis JN

    更新日期:1980-01-01 00:00:00

  • Metabolic Instruction of Immunity.

    abstract::Choices have consequences. Immune cells survey and migrate throughout the body and sometimes take residence in niche environments with distinct communities of cells, extracellular matrix, and nutrients that may differ from those in which they matured. Imbedded in immune cell physiology are metabolic pathways and metab...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.cell.2017.04.004

    authors: Buck MD,Sowell RT,Kaech SM,Pearce EL

    更新日期:2017-05-04 00:00:00

  • Glioma stem cell proliferation and tumor growth are promoted by nitric oxide synthase-2.

    abstract::Malignant gliomas are aggressive brain tumors with limited therapeutic options, and improvements in treatment require a deeper molecular understanding of this disease. As in other cancers, recent studies have identified highly tumorigenic subpopulations within malignant gliomas, known generally as cancer stem cells. H...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2011.06.006

    authors: Eyler CE,Wu Q,Yan K,MacSwords JM,Chandler-Militello D,Misuraca KL,Lathia JD,Forrester MT,Lee J,Stamler JS,Goldman SA,Bredel M,McLendon RE,Sloan AE,Hjelmeland AB,Rich JN

    更新日期:2011-07-08 00:00:00

  • Integrated systems approach identifies genetic nodes and networks in late-onset Alzheimer's disease.

    abstract::The genetics of complex disease produce alterations in the molecular interactions of cellular pathways whose collective effect may become clear through the organized structure of molecular networks. To characterize molecular systems associated with late-onset Alzheimer's disease (LOAD), we constructed gene-regulatory ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2013.03.030

    authors: Zhang B,Gaiteri C,Bodea LG,Wang Z,McElwee J,Podtelezhnikov AA,Zhang C,Xie T,Tran L,Dobrin R,Fluder E,Clurman B,Melquist S,Narayanan M,Suver C,Shah H,Mahajan M,Gillis T,Mysore J,MacDonald ME,Lamb JR,Bennett DA,

    更新日期:2013-04-25 00:00:00

  • Evolutionary history and adaptation from high-coverage whole-genome sequences of diverse African hunter-gatherers.

    abstract::To reconstruct modern human evolutionary history and identify loci that have shaped hunter-gatherer adaptation, we sequenced the whole genomes of five individuals in each of three different hunter-gatherer populations at > 60× coverage: Pygmies from Cameroon and Khoesan-speaking Hadza and Sandawe from Tanzania. We ide...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2012.07.009

    authors: Lachance J,Vernot B,Elbers CC,Ferwerda B,Froment A,Bodo JM,Lema G,Fu W,Nyambo TB,Rebbeck TR,Zhang K,Akey JM,Tishkoff SA

    更新日期:2012-08-03 00:00:00

  • Complement C1q activates canonical Wnt signaling and promotes aging-related phenotypes.

    abstract::Wnt signaling plays critical roles in development of various organs and pathogenesis of many diseases, and augmented Wnt signaling has recently been implicated in mammalian aging and aging-related phenotypes. We here report that complement C1q activates canonical Wnt signaling and promotes aging-associated decline in ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2012.03.047

    authors: Naito AT,Sumida T,Nomura S,Liu ML,Higo T,Nakagawa A,Okada K,Sakai T,Hashimoto A,Hara Y,Shimizu I,Zhu W,Toko H,Katada A,Akazawa H,Oka T,Lee JK,Minamino T,Nagai T,Walsh K,Kikuchi A,Matsumoto M,Botto M,Shiojima

    更新日期:2012-06-08 00:00:00

  • The structures and fidelity of replication of mouse mitochondrial DNA-pSC 101 EcoRI recombinant plasmids grown in E. coli K12.

    abstract::Recombinant DNAs containing the E. coli plasmid pSC101 and mouse cell (La9) mitochondrial DNA (mtDNA) were formed in vitro via ligation of DNA fragments from limit EcoRI endonuclease digests and were used to transform E. coli K12. Four structurally different recombinant plasmid DNAs from transformed clones were charac...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(76)90202-6

    authors: Brown WM,Watson RM,Vinograd J,Tait KM,Boyer HW,Goodman HM

    更新日期:1976-04-01 00:00:00

  • The Identity of Human Tissue-Emigrant CD8+ T Cells.

    abstract::Lymphocyte migration is essential for adaptive immune surveillance. However, our current understanding of this process is rudimentary, because most human studies have been restricted to immunological analyses of blood and various tissues. To address this knowledge gap, we used an integrated approach to characterize ti...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2020.11.019

    authors: Buggert M,Vella LA,Nguyen S,Wu VH,Chen Z,Sekine T,Perez-Potti A,Maldini CR,Manne S,Darko S,Ransier A,Kuri-Cervantes L,Japp AS,Brody IB,Ivarsson MA,Gorin JB,Rivera-Ballesteros O,Hertwig L,Antel JP,Johnson ME,Okoye

    更新日期:2020-12-23 00:00:00

  • A myristoyl/phosphotyrosine switch regulates c-Abl.

    abstract::The c-Abl tyrosine kinase is inhibited by mechanisms that are poorly understood. Disruption of these mechanisms in the Bcr-Abl oncoprotein leads to several forms of human leukemia. We found that like Src kinases, c-Abl 1b is activated by phosphotyrosine ligands. Ligand-activated c-Abl is particularly sensitive to the ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(03)00191-0

    authors: Hantschel O,Nagar B,Guettler S,Kretzschmar J,Dorey K,Kuriyan J,Superti-Furga G

    更新日期:2003-03-21 00:00:00

  • Localization of specificity determining sites in cadherin cell adhesion molecules.

    abstract::Cadherins are a group of homophilic intercellular adhesion molecules; each member of this family exhibits binding specificity. Here, we attempted to map the sites for the specificities of these molecules by analyzing adhesives selectivities of the cells that express chimeric and point-mutated E- and P-cadherin. The re...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(90)90222-z

    authors: Nose A,Tsuji K,Takeichi M

    更新日期:1990-04-06 00:00:00