Abstract：
:All recombination models postulate one or more recombination intermediates that are joint molecules containing two homologous parental molecules. A spike of branched DNA molecules not seen in DNA from mitotic cells was found in the two-dimensional gel analysis of meiotic DNA from S. cerevisiae. The mass of molecules in the spike, the timing of its appearance and disappearance, and its absence from a recombination-defective spo11 mutant are consistent with the hypothesis that it contains recombination intermediates. The spike changes in mass as predicted for joint molecules containing DNA from homologous chromosomes rather than sister chromatids in a strain heterozygous for an RFLP. Finally, joint molecules containing DNA from homologous chromosomes were not found, suggesting that the block to recombination between homologous sequences occurs prior to the formation of joint molecules.
journal_name
Celljournal_title
Cellauthors
Collins I,Newlon CSdoi
10.1016/0092-8674(94)90173-2subject
Has Abstractpub_date
1994-01-14 00:00:00pages
65-75issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(94)90173-2journal_volume
76pub_type
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