Abstract:
:The expression of the E2A transcription unit of the adenovirus E1A deletion mutant dl312 in murine embryonal carcinoma (EC) stem cells suggests that they contain an activity that will complement viral E1A. We have prepared from these cells in vitro transcription extracts that use E1A-inducible promoters more efficiently than do extracts from a differentiated cell line. Mixing experiments demonstrate that the EC phenotype is dominant. Gel retardation assays using the E2A promoter detect a binding activity present in F9 and PCC4 EC cells but not in differentiated cells. Our data indicate that EC stem cells contain a transcription factor that is analogous to viral E1A and is likely to be involved in the control of cellular gene expression during differentiation.
journal_name
Celljournal_title
Cellauthors
La Thangue NB,Rigby PWdoi
10.1016/0092-8674(87)90453-3subject
Has Abstractpub_date
1987-05-22 00:00:00pages
507-13issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(87)90453-3journal_volume
49pub_type
杂志文章相关文献
CELL文献大全abstract::Signaling by the epidermal growth factor receptor requires an allosteric interaction between the kinase domains of two receptors, whereby one activates the other. We show that the intracellular juxtamembrane segment of the receptor, known to potentiate kinase activity, is able to dimerize the kinase domains. The C-ter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.04.025
更新日期:2009-06-26 00:00:00
abstract::HIV release requires TSG101, a cellular factor that sorts proteins into vesicles that bud into multivesicular bodies (MVB). To test whether other proteins involved in MVB biogenesis (the class E proteins) also participate in HIV release, we identified 22 candidate human class E proteins. These proteins were connected ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00714-1
更新日期:2003-09-19 00:00:00
abstract::Radioactive alpha factor is degraded to discrete biologically inactive fragments by the target a cells of S. cerevisiae, but not by alpha cells which make the pheromone. The pattern of cleavage products and sequence analysis of one fragment indicated that the first scission occurred between leucine 6 and lysine 7. The...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90117-x
更新日期:1979-11-01 00:00:00
abstract::The COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here, we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput single-cell RNA and VDJ sequencing of antigen-enriched B cells from 60 convalescent patients. From 8,558 antigen-binding IgG1+ clonotypes, 1...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.05.025
更新日期:2020-07-09 00:00:00
abstract::There are still no effective treatments to prevent, halt, or reverse Alzheimer's disease, but research advances over the past three decades could change this gloomy picture. Genetic studies demonstrate that the disease has multiple causes. Interdisciplinary approaches combining biochemistry, molecular and cell biology...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2012.02.040
更新日期:2012-03-16 00:00:00
abstract::The relationship between activation of resting chick embryo fibroblasts by proteases and proteolytic alteration of the cell surface has been investigated. Five different proteases were examined: trypsin, collagenase, plasmin, alpha-chymotrypsin, and thrombin. All of these proteases, when added to the culture medium at...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(75)90004-5
更新日期:1975-10-01 00:00:00
abstract::The ubiquitin-specific processing protease (UBP) family of deubiquitinating enzymes plays an essential role in numerous cellular processes. HAUSP, a representative UBP, specifically deubiquitinates and hence stabilizes the tumor suppressor protein p53. Here, we report the crystal structures of the 40 kDa catalytic cor...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)01199-6
更新日期:2002-12-27 00:00:00
abstract::Human autoantibodies that recognize the spindle poles of mammals, plants, and insects were found to recognize two antigens in yeast. One of these proteins, called SPA1 (for Spindle Pole Antigen), is antigenically related to the spindle poles of a diverse set of organisms. The gene encoding SPA1 was cloned by immunoscr...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(88)90977-4
更新日期:1988-09-09 00:00:00
abstract::The chicken immunoglobulin light chain repertoire has been shown to be entirely derived from a single V lambda 1-J rearranged combination. The complete coding information of the lambda locus was determined: it comprises 25 V-hybridizing elements, all of which are pseudogenes, clustered in both orientations within 19 k...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90189-9
更新日期:1987-02-13 00:00:00
abstract::Enhancers provide critical information directing cell-type-specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here, we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.08.027
更新日期:2014-10-09 00:00:00
abstract::The X-ray crystal structures of an anti-p24 (HIV-1) monoclonal antibody Fab fragment alone and in complexes with the epitope peptide GATPQDLNTnL (n = norleucine), an epitope-homologous peptide GATPEDLNQKLAGN, as well as two unrelated peptides GLYEWGGARITNTD and efslkGpllqwrsG (D-peptide), are presented to a maximum re...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80469-9
更新日期:1997-12-12 00:00:00
abstract::Propagation of a species requires periodic cell renewal to avoid clonal senescence. Liu et al. (2010) now describe a new mechanism of cell renewal in budding yeast, in which damaged protein aggregates are transported out of the daughter buds along actin cables to preserve youthfulness. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.01.015
更新日期:2010-01-22 00:00:00
abstract::The lin-12 gene of C. elegans and the Notch gene of D. melanogaster encode structurally related transmembrane proteins that mediate intercellular signaling. We show that truncated forms of these proteins consisting of only the intracellular domains cause cell fate transformations associated with constitutive activity ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90424-o
更新日期:1993-07-30 00:00:00
abstract::Growth factors can promote cell survival by activating the phosphatidylinositide-3'-OH kinase and its downstream target, the serine-threonine kinase Akt. However, the mechanism by which Akt functions to promote survival is not understood. We show that growth factor activation of the PI3'K/Akt signaling pathway culmina...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80405-5
更新日期:1997-10-17 00:00:00
abstract::The influence of MHC antigens on TCR gamma delta usage in CD8+ intraepithelial lymphocytes (IELs) was examined using a pan-reactive and V delta 4 region-specific MAb. While an average of 30% of IELs from the majority of mice of various MHC haplotypes were V delta 4+, a 2-fold or greater percentage of IELs from H-2k mi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90166-c
更新日期:1990-10-19 00:00:00
abstract::Serum response factor (SRF) forms a ternary complex at the c-fos serum response element (SRE) with an accessory factor, Elk-1. We constructed altered-binding specificity derivatives of SRF and Elk-1 that form a ternary complex at a mutated, inactive SRE; like Elk-1, the Elk-1 variant only binds its target as part of a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90238-l
更新日期:1993-04-23 00:00:00
abstract::ATP increases microtubule steady state assembly and disassembly rates in vitro in a concentration-dependent manner. Bovine brain microtubules, composed of 75% tubulin and 25% high molecular weight microtubule-associated proteins (MAPs), were purified by three cycles of assembly and disassembly in the absence of ATP. W...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90122-3
更新日期:1979-11-01 00:00:00
abstract::We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies. In these transformants, Adh is expressed only after heat shock. Like hsp70 itself, this heat-shock-inducible Adh (Adhhs) ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90432-x
更新日期:1984-07-01 00:00:00
abstract::Insulin receptor (IR) signaling is central to normal metabolic control and dysregulated in prevalent chronic diseases. IR binds insulin at the cell surface and transduces rapid signaling via cytoplasmic kinases. However, mechanisms mediating long-term effects of insulin remain unclear. Here, we show that IR associates...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.02.030
更新日期:2019-04-18 00:00:00
abstract::We have used monoclonal antibodies to detect developmentally regulated cell surface antigens on D. discoideum amebae. A study of an antigen detected using an antibody produced by a hybridoma line implicates a previously undescribed component in the process of cell aggregation. This antigen (consisting of a doublet of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90295-7
更新日期:1983-04-01 00:00:00
abstract::Many naturalistic behaviors are built from modular components that are expressed sequentially. Although striatal circuits have been implicated in action selection and implementation, the neural mechanisms that compose behavior in unrestrained animals are not well understood. Here, we record bulk and cellular neural ac...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.04.019
更新日期:2018-06-28 00:00:00
abstract::The tumor microenvironment is known to play an important role in tumorigenesis. In this issue, Yang et al. (2008) demonstrate that mast cells heterozygous for the Nf1 gene promote the growth of neurofibromas in a mouse model of neurofibromatosis and that genetic and pharmacological inhibition of these cells is suffici...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2008.10.009
更新日期:2008-10-31 00:00:00
abstract::Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19(th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of unde...
journal_title:Cell
pub_type: 历史文章,杂志文章
doi:10.1016/j.cell.2012.01.002
更新日期:2012-01-20 00:00:00
abstract::The triskelion shape of the clathrin molecule enables it to form the polyhedral protein network that covers clathrin-coated pits and vesicles. Domains within the clathrin heavy chain that are responsible for maintaining triskelion shape and function were identified and localized. Sequences that mediate trimerization a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90033-9
更新日期:1992-03-06 00:00:00
abstract::We have isolated and characterized several members of the P transposable element family from a Drosophila melanogaster P strain. Large 2.9 kb elements are present as multiple highly conserved copies together with smaller (0.5-1.6 kb), heterogeneous elements. The complete DNA sequences of the 2.9 kb element and four sm...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90133-2
更新日期:1983-08-01 00:00:00
abstract::Spinal muscular atrophy (SMA) is an often fatal neuromuscular disease that has been directly linked to the protein product of the Survival of Motor Neurons (SMN) gene. The SMN protein is tightly associated with a novel protein, SIP1, and together they form a complex with several spliceosomal snRNP proteins. Here we sh...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80368-2
更新日期:1997-09-19 00:00:00
abstract::During development, sensory axons compete for limiting neurotrophic support, and local neurotrophin insufficiency triggers caspase-dependent axon degeneration. The signaling driving axon degeneration upon local deprivation is proposed to reside within axons. Our results instead support a model in which, despite the ap...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.01.032
更新日期:2016-02-25 00:00:00
abstract::Misfolded proteins accumulating in several neurodegenerative diseases (including Alzheimer, Parkinson, and Huntington diseases) can cause aggregation of their native counterparts through a mechanism similar to the infectious prion protein's induction of a pathogenic conformation onto its cellular isoform. Evidence for...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.10.011
更新日期:2011-10-28 00:00:00
abstract::Using a cloned cDNA complementary to a portion of the mRNA for the 50 kd human epidermal keratin, we have screened a human genomic library and have isolated and sequenced the gene encoding this keratin. A comparison of the keratin gene with the very distantly related vimentin gene has enabled us to explore the relatio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90456-2
更新日期:1984-12-01 00:00:00
abstract::The loss of expression of particular microRNAs can contribute to tumorigenesis. Kota et al. (2009) now explore in a mouse model a promising new approach for the treatment of liver cancer-re-establishing the expression of an miRNA using a viral vector. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2009.05.038
更新日期:2009-06-12 00:00:00