Abstract：
:Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19(th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of understanding tissue development, homeostasis, and disease, especially when it is combined with experimental manipulation of signals regulating cell-fate decisions. Recently, the combination of inducible recombinases, multicolor reporter constructs, and live-cell imaging has provided unprecedented insights into stem cell biology. Here we discuss the different experimental strategies currently available for lineage tracing, their associated caveats, and new opportunities to integrate lineage tracing with the monitoring of intracellular signaling pathways.
journal_name
Celljournal_title
Cellauthors
Kretzschmar K,Watt FMdoi
10.1016/j.cell.2012.01.002subject
Has Abstractpub_date
2012-01-20 00:00:00pages
33-45issue
1-2eissn
0092-8674issn
1097-4172pii
S0092-8674(12)00003-7journal_volume
148pub_type
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