Lineage tracing.


:Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19(th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of understanding tissue development, homeostasis, and disease, especially when it is combined with experimental manipulation of signals regulating cell-fate decisions. Recently, the combination of inducible recombinases, multicolor reporter constructs, and live-cell imaging has provided unprecedented insights into stem cell biology. Here we discuss the different experimental strategies currently available for lineage tracing, their associated caveats, and new opportunities to integrate lineage tracing with the monitoring of intracellular signaling pathways.






Kretzschmar K,Watt FM




Has Abstract


2012-01-20 00:00:00
















  • Neuronal Dynamics Regulating Brain and Behavioral State Transitions.

    abstract::Prolonged behavioral challenges can cause animals to switch from active to passive coping strategies to manage effort-expenditure during stress; such normally adaptive behavioral state transitions can become maladaptive in psychiatric disorders such as depression. The underlying neuronal dynamics and brainwide interac...


    pub_type: 杂志文章


    authors: Andalman AS,Burns VM,Lovett-Barron M,Broxton M,Poole B,Yang SJ,Grosenick L,Lerner TN,Chen R,Benster T,Mourrain P,Levoy M,Rajan K,Deisseroth K

    更新日期:2019-05-02 00:00:00

  • The interaction of recombination proteins with supercoiled DNA: defining the role of supercoiling in lambda integrative recombination.

    abstract::Lambda integrative recombination depends on supercoiling of the phage attachment site, attP. Using dimethylsulfate protection and indirect end-labeling, the interaction of the recombination proteins Int and IHF with supercoiled and linear attP has been studied. Supercoiling enhances the binding of Int to attP, but not...


    pub_type: 杂志文章


    authors: Richet E,Abcarian P,Nash HA

    更新日期:1986-09-26 00:00:00

  • Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes.

    abstract::NPR1 is an essential regulator of plant systemic acquired resistance (SAR), which confers immunity to a broad-spectrum of pathogens. SAR induction results in accumulation of the signal molecule salicylic acid (SA), which induces defense gene expression via activation of NPR1. We found that in an uninduced state, NPR1 ...


    pub_type: 杂志文章


    authors: Mou Z,Fan W,Dong X

    更新日期:2003-06-27 00:00:00

  • Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli.

    abstract::A general strategy is described for the isolation of suppressors of essential genes whose functions are unknown. This strategy was used to analyze the role of 4.5S RNA, an essential RNA of E. coli. In this strategy, the structural gene for 4.5S RNA is fused to the Ptac promoter in such a way that the strain becomes de...


    pub_type: 杂志文章


    authors: Brown S

    更新日期:1987-06-19 00:00:00

  • Absence epilepsy in tottering mutant mice is associated with calcium channel defects.

    abstract::Mutations at the mouse tottering (tg) locus cause a delayed-onset, recessive neurological disorder resulting in ataxia, motor seizures, and behavioral absence seizures resembling petit mal epilepsy in humans. A more severe allele, leaner (tg(la)), also shows a slow, selective degeneration of cerebellar neurons. By pos...


    pub_type: 杂志文章


    authors: Fletcher CF,Lutz CM,O'Sullivan TN,Shaughnessy JD Jr,Hawkes R,Frankel WN,Copeland NG,Jenkins NA

    更新日期:1996-11-15 00:00:00

  • p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest.

    abstract::gamma-Irradiation of human diploid fibroblasts in the G1 interval caused arrest of the cell cycle prior to S phase. This cell cycle block was correlated with a lack of activation of both cyclin E-Cyclin-dependent kinase 2 (Cdk2) and cyclin A-Cdk2 kinases and depended on wild-type p53. Although the accumulation of cycl...


    pub_type: 杂志文章


    authors: Dulić V,Kaufmann WK,Wilson SJ,Tlsty TD,Lees E,Harper JW,Elledge SJ,Reed SI

    更新日期:1994-03-25 00:00:00

  • Analysis of Human Sequence Data Reveals Two Pulses of Archaic Denisovan Admixture.

    abstract::Anatomically modern humans interbred with Neanderthals and with a related archaic population known as Denisovans. Genomes of several Neanderthals and one Denisovan have been sequenced, and these reference genomes have been used to detect introgressed genetic material in present-day human genomes. Segments of introgres...


    pub_type: 杂志文章


    authors: Browning SR,Browning BL,Zhou Y,Tucci S,Akey JM

    更新日期:2018-03-22 00:00:00

  • Autocrine VEGF signaling synergizes with EGFR in tumor cells to promote epithelial cancer development.

    abstract::It is established that tumor cell-derived VEGF acts on endothelial cells to promote angiogenesis and tumor growth. Here, we demonstrate that in K5-SOS-dependent mouse skin tumors, autocrine VEGF is required for tumor cell proliferation in a cell-autonomous and angiogenesis-independent manner. VEGF is upregulated in SO...


    pub_type: 杂志文章


    authors: Lichtenberger BM,Tan PK,Niederleithner H,Ferrara N,Petzelbauer P,Sibilia M

    更新日期:2010-01-22 00:00:00

  • Gene transfer of H-2 class II genes: antigen presentation by mouse fibroblast and hamster B-cell lines.

    abstract::We have transferred the mouse Ak alpha and Ak beta genes, which encode the class II I-Ak molecule, into mouse L-cell fibroblasts and hamster B cells. I-Ak molecules are expressed on the surface of both cell types. The L-cell and hamster B-cell I-Ak molecules appear normal by serological analyses and two-dimensional ge...


    pub_type: 杂志文章


    authors: Malissen B,Price MP,Goverman JM,McMillan M,White J,Kappler J,Marrack P,Pierres A,Pierres M,Hood L

    更新日期:1984-02-01 00:00:00

  • Suppressor mutations that restore export of a protein with a defective signal sequence.

    abstract::A selection procedure is described that should allow the genetic identification of cellular components involved in the process of protein localization in Escherichia coli. This procedure makes use of mutations that alter the signal sequence of the lambda receptor protein (product of the lamB gene), and prevent export ...


    pub_type: 杂志文章


    authors: Emr SD,Hanley-Way S,Silhavy TJ

    更新日期:1981-01-01 00:00:00

  • Small RNA species of the HeLa cell: metabolism and subcellular localization.

    abstract::The small molecular weight RNAs of the HeLa cell have been located in specific subcellular fractions. SnA is located in the nucleolus and is partially bonded to nucleolar 28S RNA. SnD, the most abundant of the small nuclear RNAs, is partially released from the nucleus when the nuclear preparation is briefly warmed. Sn...


    pub_type: 杂志文章


    authors: Zieve G,Penman S

    更新日期:1976-05-01 00:00:00

  • Adipose Tissue Macrophage-Derived Exosomal miRNAs Can Modulate In Vivo and In Vitro Insulin Sensitivity.

    abstract::MiRNAs are regulatory molecules that can be packaged into exosomes and secreted from cells. Here, we show that adipose tissue macrophages (ATMs) in obese mice secrete miRNA-containing exosomes (Exos), which cause glucose intolerance and insulin resistance when administered to lean mice. Conversely, ATM Exos obtained f...


    pub_type: 杂志文章


    authors: Ying W,Riopel M,Bandyopadhyay G,Dong Y,Birmingham A,Seo JB,Ofrecio JM,Wollam J,Hernandez-Carretero A,Fu W,Li P,Olefsky JM

    更新日期:2017-10-05 00:00:00

  • Aralar Sequesters GABA into Hyperactive Mitochondria, Causing Social Behavior Deficits.

    abstract::Social impairment is frequently associated with mitochondrial dysfunction and altered neurotransmission. Although mitochondrial function is crucial for brain homeostasis, it remains unknown whether mitochondrial disruption contributes to social behavioral deficits. Here, we show that Drosophila mutants in the homolog ...


    pub_type: 杂志文章


    authors: Kanellopoulos AK,Mariano V,Spinazzi M,Woo YJ,McLean C,Pech U,Li KW,Armstrong JD,Giangrande A,Callaerts P,Smit AB,Abrahams BS,Fiala A,Achsel T,Bagni C

    更新日期:2020-03-19 00:00:00

  • Expression of cDNA encoding the human "protective protein" associated with lysosomal beta-galactosidase and neuraminidase: homology to yeast proteases.

    abstract::The "protective protein" is a glycoprotein that associates with lysosomal beta-galactosidase and neuraminidase and is deficient in the autosomal recessive disorder galactosialidosis. We have isolated the cDNA encoding human "protective protein". The clone recognizes a 2 kb mRNA in normal cells that is not evident in f...


    pub_type: 杂志文章


    authors: Galjart NJ,Gillemans N,Harris A,van der Horst GT,Verheijen FW,Galjaard H,d'Azzo A

    更新日期:1988-09-09 00:00:00

  • Decoding neural circuits that control compulsive sucrose seeking.

    abstract::The lateral hypothalamic (LH) projection to the ventral tegmental area (VTA) has been linked to reward processing, but the computations within the LH-VTA loop that give rise to specific aspects of behavior have been difficult to isolate. We show that LH-VTA neurons encode the learned action of seeking a reward, indepe...


    pub_type: 杂志文章


    authors: Nieh EH,Matthews GA,Allsop SA,Presbrey KN,Leppla CA,Wichmann R,Neve R,Wildes CP,Tye KM

    更新日期:2015-01-29 00:00:00

  • Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.

    abstract::Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with ...


    pub_type: 杂志文章


    authors: Dimitrova YN,Jenni S,Valverde R,Khin Y,Harrison SC

    更新日期:2016-11-03 00:00:00

  • The relationship of IL-4- and IFN gamma-producing T cells studied by lineage ablation of IL-4-producing cells.

    abstract::Subsets of CD4 T cells are defined by the cytokines that they produce; these cytokines determine the effector function of these cells. Cloned CD4 T cells fall into two subsets, producing either interferon-gamma (IFN gamma) or interleukin-4 (IL-4) in combination with other cytokines, and are called Th1 and Th2 cells, r...


    pub_type: 杂志文章


    authors: Kamogawa Y,Minasi LA,Carding SR,Bottomly K,Flavell RA

    更新日期:1993-12-03 00:00:00

  • Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.

    abstract::Genomic Xenopus borealis oocyte-specific 5S DNA (Xbo) contains clusters of 5S rRNA genes. The number of genes varies among clusters, and the distance between genes within a cluster is about 80 nucleotides. The spacer DNA between gene clusters is AT-rich and heterogeneous in length due in part to variable numbers of a ...


    pub_type: 杂志文章


    authors: Korn LJ,Brown DD

    更新日期:1978-12-01 00:00:00

  • A CK2-dependent mechanism for degradation of the PML tumor suppressor.

    abstract::The PML tumor suppressor controls key pathways for growth suppression, induction of apoptosis, and cellular senescence. PML loss occurs frequently in human tumors through unknown posttranslational mechanisms. Casein kinase 2 (CK2) is oncogenic and frequently upregulated in human tumors. Here we show that CK2 regulates...


    pub_type: 杂志文章


    authors: Scaglioni PP,Yung TM,Cai LF,Erdjument-Bromage H,Kaufman AJ,Singh B,Teruya-Feldstein J,Tempst P,Pandolfi PP

    更新日期:2006-07-28 00:00:00

  • Ubiquitous transcription factor OTF-1 mediates induction of the MMTV promoter through synergistic interaction with hormone receptors.

    abstract::Steroid hormones induce transcription from the mouse mammary tumor virus (MMTV) promoter by complex mechanisms requiring binding of the hormone receptors to the hormone responsive element (HRE) of the long terminal repeat region. Here we show that the MMTV promoter contains two degenerated octamer motifs immediately u...


    pub_type: 杂志文章


    authors: Brüggemeier U,Kalff M,Franke S,Scheidereit C,Beato M

    更新日期:1991-02-08 00:00:00

  • BACH1, a novel helicase-like protein, interacts directly with BRCA1 and contributes to its DNA repair function.

    abstract::BRCA1 interacts in vivo with a novel protein, BACH1, a member of the DEAH helicase family. BACH1 binds directly to the BRCT repeats of BRCA1. A BACH1 derivative, bearing a mutation in a residue that was essential for catalytic function in other helicases, interfered with normal double-strand break repair in a manner t...


    pub_type: 杂志文章


    authors: Cantor SB,Bell DW,Ganesan S,Kass EM,Drapkin R,Grossman S,Wahrer DC,Sgroi DC,Lane WS,Haber DA,Livingston DM

    更新日期:2001-04-06 00:00:00

  • Immunosurveillance by hematopoietic progenitor cells trafficking through blood, lymph, and peripheral tissues.

    abstract::Constitutive egress of bone marrow (BM)-resident hematopoietic stem and progenitor cells (HSPCs) into the blood is a well-established phenomenon, but the ultimate fate and functional relevance of circulating HSPCs is largely unknown. We show that mouse thoracic duct (TD) lymph contains HSPCs that possess short- and lo...


    pub_type: 杂志文章


    authors: Massberg S,Schaerli P,Knezevic-Maramica I,Köllnberger M,Tubo N,Moseman EA,Huff IV,Junt T,Wagers AJ,Mazo IB,von Andrian UH

    更新日期:2007-11-30 00:00:00

  • A signaling principle for the specification of the germ cell lineage in mice.

    abstract::Specification of the germ cell lineage is vital to development and heredity. In mice, the germ cell fate is induced in pluripotent epiblast cells by signaling molecules, yet the underlying mechanism remains unknown. Here we demonstrate that germ cell fate in the epiblast is a direct consequence of Bmp4 signaling from ...


    pub_type: 杂志文章


    authors: Ohinata Y,Ohta H,Shigeta M,Yamanaka K,Wakayama T,Saitou M

    更新日期:2009-05-01 00:00:00

  • Mobility of histones on the chromosome of simian virus 40.

    abstract::Linear simian virus 40 (SV40) chromosomes were prepared by Eco R1 nuclease cleavage of the circular SV40 chromosomes released from virions with dithiothreitol at pH 9,8. Chromatin-DNA hybrids were constructed with segments of 3H-labeled, naked SV40 DNA covalently joined via the Eco R1-generated cohesive ends to segmen...


    pub_type: 杂志文章


    authors: Beard P

    更新日期:1978-11-01 00:00:00

  • The proton flux through the bacterial flagellar motor.

    abstract::Bacterial flagella are driven by a rotary motor that utilizes the free energy stored in the electrochemical proton gradient across the cytoplasmic membrane to do mechanical work. The flux of protons coupled to motor rotation was measured in Streptococcus and found to be directly proportional to motor speed. This suppo...


    pub_type: 杂志文章


    authors: Meister M,Lowe G,Berg HC

    更新日期:1987-06-05 00:00:00

  • Microheterogeneity among carbohydrate structures at the cell surface may be important in recognition phenomena.

    abstract::Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogenei...


    pub_type: 杂志文章


    authors: Stanley P,Sudo T

    更新日期:1981-03-01 00:00:00

  • Proline isomerization of histone H3 regulates lysine methylation and gene expression.

    abstract::The cis-trans isomerization of proline serves as a regulatory switch in signaling pathways. We identify the proline isomerase Fpr4, a member of the FK506 binding protein family in Saccharomyces cerevisiae, as an enzyme which binds the amino-terminal tail of histones H3 and H4 and catalyses the isomerization of H3 prol...


    pub_type: 杂志文章


    authors: Nelson CJ,Santos-Rosa H,Kouzarides T

    更新日期:2006-09-08 00:00:00

  • Ig Superfamily Ligand and Receptor Pairs Expressed in Synaptic Partners in Drosophila.

    abstract::Information processing relies on precise patterns of synapses between neurons. The cellular recognition mechanisms regulating this specificity are poorly understood. In the medulla of the Drosophila visual system, different neurons form synaptic connections in different layers. Here, we sought to identify candidate ce...


    pub_type: 杂志文章


    authors: Tan L,Zhang KX,Pecot MY,Nagarkar-Jaiswal S,Lee PT,Takemura SY,McEwen JM,Nern A,Xu S,Tadros W,Chen Z,Zinn K,Bellen HJ,Morey M,Zipursky SL

    更新日期:2015-12-17 00:00:00

  • Cellular uptake of the tat protein from human immunodeficiency virus.

    abstract::While developing an assay to measure the activity of the tat protein from human immunodeficiency virus 1 (HIV-1), we discovered that the purified protein could be taken up by cells growing in tissue culture and subsequently trans-activate the viral promoter. Trans-activation is dramatically increased by a variety of l...


    pub_type: 杂志文章


    authors: Frankel AD,Pabo CO

    更新日期:1988-12-23 00:00:00

  • The synthesis and distribution of desmin and vimentin during myogenesis in vitro.

    abstract::Electrophoretic and autoradiographic analyses of the incorporation of 35S--methionine into newly synthesized proteins during myogenesis reveal that presumptive chicken myoblasts synthesize primarily one intermediate filament protein: vimentin. Desmin synthesis is initiated at the onset of fusion. Synthesis rates of bo...


    pub_type: 杂志文章


    authors: Gard DL,Lazarides E

    更新日期:1980-01-01 00:00:00