Mutational analysis of HIV-1 Tat minimal domain peptides: identification of trans-dominant mutants that suppress HIV-LTR-driven gene expression.

abstract：:We studied H1 gene expression in hybrids of Xenopus laevis (female) x Xenopus borealis (male) and saw paternal H1 synthesis in mid-blastulae, which indicates that the H1 genes are active by this stage. The behavior of the maternal store of H1 histone mRNA was studied in androgenetic haploids. In which all stored mRNA ...

journal_title：Cell

authors： Woodland HR,Flynn JM,Wyllie AJ

更新日期：1979-09-01 00:00:00

abstract：:Telomeres of Oxytricha nova macronuclear chromosomes consist of a repeated T4G4 sequence, single-stranded at the 3' terminus, bound by a heterodimeric protein. The cloning of genes for the two polypeptides and their separate expression in E. coli have enabled evaluation of their individual contributions to DNA binding...

journal_title：Cell

authors： Gray JT,Celander DW,Price CM,Cech TR

更新日期：1991-11-15 00:00:00

abstract：:The mechanisms by which molecular chaperones assist quality control of cytosolic proteins are poorly understood. Analysis of the chaperone requirements for degradation of misfolded variants of a cytosolic protein, the VHL tumor suppressor, reveals that distinct chaperone pathways mediate its folding and quality contro...

journal_title：Cell

authors： McClellan AJ,Scott MD,Frydman J

更新日期：2005-06-03 00:00:00

abstract：:Using a defined RNA polymerase II (pol II) transcription system, we have investigated the roles of basal factors at discrete stages during the transcription cycle (e.g., initiation, promoter clearance, and transcript elongation). Abortive initiation assays revealed that TATA-binding protein, transcription factors TFII...

journal_title：Cell

authors： Goodrich JA,Tjian R

更新日期：1994-04-08 00:00:00

abstract：:A nutritious diet low in calories improves the health and extends the life span of rodents. Recent studies identified a gene, SIR2, which encodes an NAD-dependent deacetylase and may mediate the effects of calorie restriction. In this review, we discuss SIR2 genes and calorie restriction in the lower organisms yeast a...

journal_title：Cell

authors： Guarente L,Picard F

更新日期：2005-02-25 00:00:00

abstract：:The general transcription factor TFIID is a multiprotein complex containing the TATA-binding protein and several associated factors (TAFs), some of which may function as coactivators that are essential for activated, but not basal, transcription. Here we describe the isolation and characterization of the first gene en...

journal_title：Cell

authors： Hoey T,Weinzierl RO,Gill G,Chen JL,Dynlacht BD,Tjian R

更新日期：1993-01-29 00:00:00

abstract：:The kinetics of association of epidermal growth factor (EGF) and diferric-transferrin (TF) with clathrin-coated membranes of KB cells were examined using anti-clathrin antibody bound to Staphylococcus aureus. The ligands were bound to cells at 4 degrees C and after warming to 30 degrees C for 5 min, both ligands were ...

journal_title：Cell

authors： Hanover JA,Willingham MC,Pastan I

更新日期：1984-12-01 00:00:00

abstract：:After translational termination, mRNA and P site deacylated tRNA remain associated with ribosomes in posttermination complexes (post-TCs), which must therefore be recycled by releasing mRNA and deacylated tRNA and by dissociating ribosomes into subunits. Recycling of bacterial post-TCs requires elongation factor EF-G ...

journal_title：Cell

authors： Pisarev AV,Hellen CU,Pestova TV

更新日期：2007-10-19 00:00:00

abstract：:Individual olfactory sensory neurons express only a single odorant receptor from a large family of genes, and this singularity is an essential feature in models of olfactory perception. We have devised a genetic strategy to examine the stability of receptor choice. We observe that immature olfactory sensory neurons th...

journal_title：Cell

authors： Shykind BM,Rohani SC,O'Donnell S,Nemes A,Mendelsohn M,Sun Y,Axel R,Barnea G

更新日期：2004-06-11 00:00:00

abstract：:Polyglycylation is a posttranslational modification that generates glycine side chains on proteins. Here we identify a family of evolutionarily conserved glycine ligases that modify tubulin using different enzymatic mechanisms. In mammals, two distinct enzyme types catalyze the initiation and elongation steps of polyg...

journal_title：Cell

authors： Rogowski K,Juge F,van Dijk J,Wloga D,Strub JM,Levilliers N,Thomas D,Bré MH,Van Dorsselaer A,Gaertig J,Janke C

更新日期：2009-06-12 00:00:00

abstract：:The mRNAs from two yeast mitochondrial genes cob-box (cytochrome b) and oxi-3 (cytochrome oxidase 40,000 dalton subunit) are processed from large (7-10 kb) precursors. Certain mutations in each gene block the maturation of the RNAs from both genes at a variety of specific steps. The pleiotropic cytochrome b mutants se...

journal_title：Cell

authors： Church GM,Slonimski PP,Gilbert W

更新日期：1979-12-01 00:00:00

abstract：:In eukaryotes, a specialized pathway of mRNA degradation termed nonsense-mediated decay (NMD) functions in mRNA quality control by recognizing and degrading mRNAs with aberrant termination codons. We demonstrate that NMD in yeast targets premature termination codon (PTC)-containing mRNA to P-bodies. Upf1p is sufficien...

journal_title：Cell

authors： Sheth U,Parker R

更新日期：2006-06-16 00:00:00

abstract：:We here attempt to achieve an integrated understanding of the structure and dynamics of a number of higher-order assemblies, including amyloids, various kinds of signalosomes, and cellular granules. We propose that the synergy between folded domains, linear motifs, and intrinsically disordered regions regulates the fo...

journal_title：Cell

authors： Wu H,Fuxreiter M

更新日期：2016-05-19 00:00:00

abstract：:Expression of human immunodeficiency virus type 1 structural proteins requires both the viral Rev trans-activator and its cis-acting RNA target sequence, the Rev response element (RRE). The RRE has been mapped to a conserved region of the HIV-1 env gene and is predicted to form a complex, highly stable RNA stem-loop s...

journal_title：Cell

authors： Malim MH,Tiley LS,McCarn DF,Rusche JR,Hauber J,Cullen BR

更新日期：1990-02-23 00:00:00

abstract：:The v-erbB gene of avian erythroblastosis virus (AEV) codes for an integral plasma membrane glycoprotein, gp74erbB. Expression of gp74erbB and its intracellular precursors, gp66erbB and gp68erbB, has been studied in cells transformed by two temperature-sensitive mutants of AEV. After shift to 42 degrees C, the process...

journal_title：Cell

authors： Beug H,Hayman MJ

更新日期：1984-04-01 00:00:00

abstract：:Pathogenic and other cytoplasmic DNAs activate the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway to induce inflammation via transcriptional activation by IRF3 and nuclear factor κB (NF-κB), but the functional consequences of exposing cGAS to chromosomes upon mitotic nuclear envelope bre...

journal_title：Cell

authors： Zierhut C,Yamaguchi N,Paredes M,Luo JD,Carroll T,Funabiki H

更新日期：2019-07-11 00:00:00

abstract：:This year's Albert Lasker Basic Medical Research Award honors David Allis and Michael Grunstein for their pioneering research that highlighted the importance of histones and their post-translational modifications in the direct control of gene expression. ...

journal_title：Cell

authors： Stillman B

更新日期：2018-09-20 00:00:00

abstract：:As visualized by electron microscopy, RecA protein binds in a highly cooperative manner to single-stranded fd DNA in solutions of 0.01 M Tris (pH 7.5). The resulting nucleoprotein filament loops are 1.25 micrometers in length, have a fiber diameter of 12 nm and show an indication of a 4.5 nm repeat along the axis of t...

journal_title：Cell

authors： Dunn K,Chrysogelos S,Griffith J

更新日期：1982-04-01 00:00:00

abstract：:Interactions between similar but not identical (homeologous) DNA sequences play an important biological role in the evolution of genes and genomes. To gain insight into the underlying molecular mechanism(s) of genetic recombination, we have studied inter- and intramolecular homeologous recombination in S. cerevisiae d...

journal_title：Cell

authors： Mézard C,Pompon D,Nicolas A

更新日期：1992-08-21 00:00:00

abstract：:In prokaryotes, DNA can be segregated by three different types of cytoskeletal filaments. The best-understood type of partitioning (par) locus encodes an actin homolog called ParM, which forms dynamically unstable filaments that push plasmids apart in a process reminiscent of mitosis. However, the most common type of ...

journal_title：Cell

authors： Gerdes K,Howard M,Szardenings F

更新日期：2010-06-11 00:00:00

abstract：:Two studies from Jarosz et al. describe how [GAR(+)], a protein-based epigenetic determinant found mainly in wild yeast strains, can be activated by microbial cross-kingdom communication. With the aid of genetically and ecologically diverse bacteria, yeast can override an ancient regulatory mechanism of glucose repres...

journal_title：Cell

authors： Staniforth GL,Tuite MF

更新日期：2014-08-28 00:00:00

abstract：:The pandemic has impacted every scientist differently. Many negative impacts are frequently discussed. Here we highlight unexpected positives that we have found and hope will persist: improved access to experts; deeper and broader human engagement among colleagues, collaborators, and competitors; and significant democ...

journal_title：Cell

authors： Arturo EC,Saphire EO

更新日期：2020-10-01 00:00:00

abstract：:Computational modeling and the theory of nonlinear dynamical systems allow one to not simply describe the events of the cell cycle, but also to understand why these events occur, just as the theory of gravitation allows one to understand why cannonballs fly in parabolic arcs. The simplest examples of the eukaryotic ce...

journal_title：Cell

authors： Ferrell JE Jr,Tsai TY,Yang Q

更新日期：2011-03-18 00:00:00

abstract：:Homologous recombination in embryonal stem cells has been used to produce a fusion oncogene, thereby mimicking chromosomal translocations that frequently result in formation of tumor-specific fusion oncogenes in human malignancies. AF9 sequences were fused into the mouse Mll gene so that expression of the Mll-AF9 fusi...

journal_title：Cell

authors： Corral J,Lavenir I,Impey H,Warren AJ,Forster A,Larson TA,Bell S,McKenzie AN,King G,Rabbitts TH

更新日期：1996-06-14 00:00:00

abstract：:A comprehensive analysis uncovered a set of yeast proteins promoting protein-based inheritance that shares many of the non-Mendelian properties of prions. Lacking any sequence or structural signatures of known prions, these proteins represent a new class of non-amyloid, protein-based epigenetic determinants that can c...

journal_title：Cell

authors： Tuite MF

更新日期：2016-10-06 00:00:00

abstract：:Defects in the gene encoding the endothelin-B receptor produce aganglionic megacolon and pigmentary disorders in mice and humans. We report that a targeted disruption of the mouse endothelin-3 ligand (EDN3) gene produces a similar recessive phenotype of megacolon and coat color spotting. A natural recessive mutation t...

journal_title：Cell

authors： Baynash AG,Hosoda K,Giaid A,Richardson JA,Emoto N,Hammer RE,Yanagisawa M

更新日期：1994-12-30 00:00:00

abstract：:Procollagen and collagen were isolated from the culture medium and cell layer of line TSD4 (obtained from mouse teratocarcinoma OTT6050). SDS-polyacrylamide gel electrophoresis of the highly purified procollagen fraction demonstrated that the fraction is composed of theta chains (150,000 daltons), pro alpha chains (13...

journal_title：Cell

authors： Little CD,Church RL,Miller RA,Ruddle FH

更新日期：1977-02-01 00:00:00

abstract：:Mouse monoclonal antibody 4-I-244 detects a developmentally regulated antigen in embryos of Volvox carteri and inhibits specifically the morphogenetic process of inversion (the process by which the embryo turns inside out). Antigen 4-I-244 was chemically characterized as a complex phytosphingolipid containing the neut...

journal_title：Cell

authors： Wenzl S,Sumper M

更新日期：1986-08-15 00:00:00

abstract：:A major problem in treatment of cancers arising in steroid-sensitive cells is their inevitable progression to a steroid-insensitive state; current therapies are based on the assumption that hormone insensitivity is associated with loss of receptor. We demonstrate for the first time that breast tumor cells can progress...

journal_title：Cell

authors： Darbre PD,King RJ

更新日期：1987-11-20 00:00:00

abstract：:Eukaryotic Argonaute proteins strictly mediate RNA-guided RNA interference. In contrast, prokaryotic Argonautes can utilize DNA guides to target complementary DNA sequences to protect their hosts against invading DNA. In this issue of Cell, Jolly and colleagues demonstrate that Thermus thermophilus Argonaute additiona...

journal_title：Cell

authors： Swarts DC

更新日期：2020-09-17 00:00:00