Expression in rat fibroblasts of a human transforming growth factor-alpha cDNA results in transformation.

abstract：:Many organisms expand the information content of their genome through enzymatic methylation of cytosine residues. Here we report the 2.8 A crystal structure of a bacterial DNA (cytosine-5)-methyltransferase (DCMtase), M. HaeIII, bound covalently to DNA. In this complex, the substrate cytosine is extruded from the DNA ...

journal_title：Cell

authors： Reinisch KM,Chen L,Verdine GL,Lipscomb WN

更新日期：1995-07-14 00:00:00

abstract：:Isolated human mitochondria containing a mitochondrial DNA (mtDNA) coded chloramphenicol resistance marker were injected into cells from two different human sensitive cell lines, 143BTK- and HT1080-6TG, which had been partially depleted of their mtDNA by ethidium bromide treatment. On the basis of the available eviden...

journal_title：Cell

authors： King MP,Attardi G

更新日期：1988-03-25 00:00:00

abstract：:Prolonged behavioral challenges can cause animals to switch from active to passive coping strategies to manage effort-expenditure during stress; such normally adaptive behavioral state transitions can become maladaptive in psychiatric disorders such as depression. The underlying neuronal dynamics and brainwide interac...

journal_title：Cell

authors： Andalman AS,Burns VM,Lovett-Barron M,Broxton M,Poole B,Yang SJ,Grosenick L,Lerner TN,Chen R,Benster T,Mourrain P,Levoy M,Rajan K,Deisseroth K

更新日期：2019-05-02 00:00:00

abstract：:A safe and controlled manipulation of endocytosis in vivo may have disruptive therapeutic potential. Here, we demonstrate that the anti-emetic/anti-psychotic prochlorperazine can be repurposed to reversibly inhibit the in vivo endocytosis of membrane proteins targeted by therapeutic monoclonal antibodies, as directly ...

journal_title：Cell

authors： Chew HY,De Lima PO,Gonzalez Cruz JL,Banushi B,Echejoh G,Hu L,Joseph SR,Lum B,Rae J,O'Donnell JS,Merida de Long L,Okano S,King B,Barry R,Moi D,Mazzieri R,Thomas R,Souza-Fonseca-Guimaraes F,Foote M,McCluskey A,Robin

更新日期：2020-03-05 00:00:00

abstract：:In the mammalian brain, the specification of a single axon and multiple dendrites occurs early in the differentiation of most neuron types. Numerous intracellular signaling events for axon specification have been described in detail. However, the identity of the extracellular factor(s) that initiate neuronal polarity ...

journal_title：Cell

authors： Yi JJ,Barnes AP,Hand R,Polleux F,Ehlers MD

更新日期：2010-07-09 00:00:00

abstract：:Drosophila Reaper (RPR), Head Involution Defective (HID), and GRIM induce caspase-dependent cell death and physically interact with the cell death inhibitor DIAP1. Here we show that HID blocks DIAP1's ability to inhibit caspase activity and provide evidence suggesting that RPR and GRIM can act similarly. Based on thes...

journal_title：Cell

authors： Wang SL,Hawkins CJ,Yoo SJ,Müller HA,Hay BA

更新日期：1999-08-20 00:00:00

abstract：:Recent work has identified a mouse gene (tsg101) whose inactivation in fibroblasts results in cellular transformation and the ability to produce metastatic tumors in nude mice. Here, we report that the human homolog, TSG101, which we isolated and mapped to chromosome 11, bands 15.1-15.2, a region proposed to contain t...

journal_title：Cell

authors： Li L,Li X,Francke U,Cohen SN

更新日期：1997-01-10 00:00:00

abstract：:DNA fragments containing the silencers that flank the mating type genes at HML alpha are shown to bind specifically to the nuclear scaffold of yeast. The scaffold proteins are solubilized with urea and then renatured to form a soluble extract which allows reconstitution of sequence-specific DNA loops. At the silent ma...

journal_title：Cell

authors： Hofmann JF,Laroche T,Brand AH,Gasser SM

更新日期：1989-06-02 00:00:00

abstract：:The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking ...

journal_title：Cell

authors： Pardee K,Green AA,Takahashi MK,Braff D,Lambert G,Lee JW,Ferrante T,Ma D,Donghia N,Fan M,Daringer NM,Bosch I,Dudley DM,O'Connor DH,Gehrke L,Collins JJ

更新日期：2016-05-19 00:00:00

abstract：:Adult skeletal muscle generates force in a controlled and directed manner through the contraction of highly specialized, postmitotic, multinucleated myofibers. Life-long muscle function relies on maintenance and regeneration of myofibers through a highly regulated process beginning with activation of normally quiescen...

journal_title：Cell

authors： Wagers AJ,Conboy IM

更新日期：2005-09-09 00:00:00

abstract：:The structure of chicken erythrocyte and chicken liver chromatin has been studied by enzymatic digestion with micrococcal nuclease and DNAase I. Comparison of the fragments produced by brief digestion with micrococcal nuclease indicates that chicken erythrocyte chromatin has a longer nucleosome repeat than chicken liv...

journal_title：Cell

authors： Morris NR

更新日期：1976-12-01 00:00:00

abstract：:A new rhythm mutation was isolated based on its elimination of per-controlled luciferase cycling. Levels of period or timeless clock gene products in the mutant are flat in daily light-dark cycles or constant darkness (although PER and TIM oscillate normally in temperature cycles). Consistent with the fact that light ...

journal_title：Cell

authors： Stanewsky R,Kaneko M,Emery P,Beretta B,Wager-Smith K,Kay SA,Rosbash M,Hall JC

更新日期：1998-11-25 00:00:00

abstract：:To investigate the role of the presumed DNA mismatch repair (MMR) gene Msh2 in genome stability and tumorigenesis, we have generated cells and mice that are deficient for the gene. Msh2-deficient cells have lost mismatch binding and have acquired microsatellite instability, a mutator phenotype, and tolerance to methyl...

journal_title：Cell

authors： de Wind N,Dekker M,Berns A,Radman M,te Riele H

更新日期：1995-07-28 00:00:00

abstract：:Cortical layer 1 (L1) interneurons have been proposed as a hub for attentional modulation of underlying cortex, but the transformations that this circuit implements are not known. We combined genetically targeted voltage imaging with optogenetic activation and silencing to study the mechanisms underlying sensory proce...

journal_title：Cell

authors： Fan LZ,Kheifets S,Böhm UL,Wu H,Piatkevich KD,Xie ME,Parot V,Ha Y,Evans KE,Boyden ES,Takesian AE,Cohen AE

更新日期：2020-02-06 00:00:00

abstract：:Motile cells extend a leading edge by assembling a branched network of actin filaments that produces physical force as the polymers grow beneath the plasma membrane. A core set of proteins including actin, Arp2/3 complex, profilin, capping protein, and ADF/cofilin can reconstitute the process in vitro, and mathematica...

journal_title：Cell

authors： Pollard TD,Borisy GG

更新日期：2003-02-21 00:00:00

abstract：:Evasion of DNA damage-induced cell death, via mutation of the p53 tumor suppressor or overexpression of prosurvival Bcl-2 family proteins, is a key step toward malignant transformation and therapeutic resistance. We report that depletion or acute inhibition of checkpoint kinase 1 (Chk1) is sufficient to restore gamma-...

journal_title：Cell

authors： Sidi S,Sanda T,Kennedy RD,Hagen AT,Jette CA,Hoffmans R,Pascual J,Imamura S,Kishi S,Amatruda JF,Kanki JP,Green DR,D'Andrea AA,Look AT

更新日期：2008-05-30 00:00:00

abstract：:SecA, the peripheral subunit of E. coli preprotein translocase, alternates between a membrane inserted and a deinserted state as part of the catalytic cycle of preprotein translocation. When SecA is complexed with SecY/E and preprotein, ATP drives a profound conformational change, leading to membrane insertion of a 30...

journal_title：Cell

authors： Economou A,Wickner W

更新日期：1994-09-09 00:00:00

abstract：:The endoplasmic reticulum (ER) often forms stacked membrane sheets, an arrangement that is likely required to accommodate a maximum of membrane-bound polysomes for secretory protein synthesis. How sheets are stacked is unknown. Here, we used improved staining and automated ultrathin sectioning electron microscopy meth...

journal_title：Cell

authors： Terasaki M,Shemesh T,Kasthuri N,Klemm RW,Schalek R,Hayworth KJ,Hand AR,Yankova M,Huber G,Lichtman JW,Rapoport TA,Kozlov MM

更新日期：2013-07-18 00:00:00

abstract：:Enveloped viruses enter cells by inducing fusion of viral and cellular membranes, a process catalyzed by a specialized membrane-fusion protein expressed on their surface. This review focuses on recent structural studies of viral fusion proteins with an emphasis on their metastable prefusion form and on interactions wi...

journal_title：Cell

authors： Rey FA,Lok SM

更新日期：2018-03-08 00:00:00

abstract：:The sphingomyelin pathway, which is initiated by sphingomyelin hydrolysis to generate the second messenger ceramide, signals apoptosis for tumor necrosis factor alpha, Fas, and ionizing radiation. In the present studies, the anticancer drug daunorubicin also stimulated ceramide elevation and apoptosis in P388 and U937...

journal_title：Cell

authors： Bose R,Verheij M,Haimovitz-Friedman A,Scotto K,Fuks Z,Kolesnick R

更新日期：1995-08-11 00:00:00

abstract：:PD-1 immune checkpoint blockade provides significant clinical benefits for melanoma patients. We analyzed the somatic mutanomes and transcriptomes of pretreatment melanoma biopsies to identify factors that may influence innate sensitivity or resistance to anti-PD-1 therapy. We find that overall high mutational loads a...

journal_title：Cell

authors： Hugo W,Zaretsky JM,Sun L,Song C,Moreno BH,Hu-Lieskovan S,Berent-Maoz B,Pang J,Chmielowski B,Cherry G,Seja E,Lomeli S,Kong X,Kelley MC,Sosman JA,Johnson DB,Ribas A,Lo RS

更新日期：2016-03-24 00:00:00

abstract：:Much of the Y chromosome consists of large palindromic arrays harboring genes that are critical for spermatogenesis. In this issue, Lange et al. (2009) show that although gene conversion within these arrays maintains their integrity, it also permits rare unequal sister chromatid-exchange events within palindromes that...

journal_title：Cell

authors： Hall HE,Hawley RS

更新日期：2009-09-04 00:00:00

abstract：:A major challenge to understanding how cells work together in the central nervous system (CNS) is the heterogeneous cellular composition of the brain. In this issue, Heiman et al. (2008) and Doyle et al. (2008) introduce a new strategy (TRAP) that enables the profiling of translated mRNAs in specific CNS cell populati...

journal_title：Cell

authors： Emery B,Barres BA

更新日期：2008-11-14 00:00:00

abstract：:Pathogenic simian immunodeficiency virus (SIV) infection is associated with enteropathy, which likely contributes to AIDS progression. To identify candidate etiologies for AIDS enteropathy, we used next-generation sequencing to define the enteric virome during SIV infection in nonhuman primates. Pathogenic, but not no...

journal_title：Cell

authors： Handley SA,Thackray LB,Zhao G,Presti R,Miller AD,Droit L,Abbink P,Maxfield LF,Kambal A,Duan E,Stanley K,Kramer J,Macri SC,Permar SR,Schmitz JE,Mansfield K,Brenchley JM,Veazey RS,Stappenbeck TS,Wang D,Barouch DH,

更新日期：2012-10-12 00:00:00

abstract：:Salmonella enterica invasion of host cells requires the reversible activation of the Rho-family GTPases Cdc42 and Rac1 by the bacterially encoded GEF SopE and the GAP SptP, which exert their function at different times during infection and are delivered into host cells by a type III secretion system. We found that Sop...

journal_title：Cell

authors： Kubori T,Galán JE

更新日期：2003-10-31 00:00:00

abstract：:A mutation in any one of eight spoO genes of Bacillus subtilis blocks the process of spore formation at its earliest stage. To investigate how the products of the spoO genes may be involved in developmental gene expression, we fused the lacZ gene of E. coli to spoVG, a sporulation gene whose induction at the onset of ...

journal_title：Cell

authors： Zuber P,Losick R

更新日期：1983-11-01 00:00:00

abstract：:The mechanisms by which immune checkpoint blockade modulates tumor evolution during therapy are unclear. We assessed genomic changes in tumors from 68 patients with advanced melanoma, who progressed on ipilimumab or were ipilimumab-naive, before and after nivolumab initiation (CA209-038 study). Tumors were analyzed by...

journal_title：Cell

authors： Riaz N,Havel JJ,Makarov V,Desrichard A,Urba WJ,Sims JS,Hodi FS,Martín-Algarra S,Mandal R,Sharfman WH,Bhatia S,Hwu WJ,Gajewski TF,Slingluff CL Jr,Chowell D,Kendall SM,Chang H,Shah R,Kuo F,Morris LGT,Sidhom JW,Sch

更新日期：2017-11-02 00:00:00

abstract：:32P-labeled cowpea mosaic virus (CPMV) RNAs were analyzed for their 5' termini by three different fingerprinting standard procedures. These analyses showed unequivocally that CPMV RNAs lack a structure like m7GpppN ... at their 5' termini. In addition, neither mono-, di- or triphosphorylated [(p)(p)pN ....] termini we...

journal_title：Cell

authors： Klootwijk J,Klein I,Zabel P,van Kammen A

更新日期：1977-05-01 00:00:00

abstract：:Ischemia-associated oxidative damage leading to necrosis is a major cause of catastrophic tissue loss, and elucidating its signaling mechanism is therefore of paramount importance. p53 is a central stress sensor responding to multiple insults, including oxidative stress to orchestrate apoptotic and autophagic cell dea...

journal_title：Cell

authors： Vaseva AV,Marchenko ND,Ji K,Tsirka SE,Holzmann S,Moll UM

更新日期：2012-06-22 00:00:00

abstract：:Gastric acid secretion is mediated by the H/K-ATPase of parietal cells. Activation of acid secretion involves insertion of H/K-ATPase into the parietal cell plasmalemma, while its cessation is associated with reinternalization of the H/K-ATPase into an intracellular storage compartment. The cytoplasmic tail of the H/K...

journal_title：Cell

authors： Courtois-Coutry N,Roush D,Rajendran V,McCarthy JB,Geibel J,Kashgarian M,Caplan MJ

更新日期：1997-08-08 00:00:00