Abstract:
:The structure of chicken erythrocyte and chicken liver chromatin has been studied by enzymatic digestion with micrococcal nuclease and DNAase I. Comparison of the fragments produced by brief digestion with micrococcal nuclease indicates that chicken erythrocyte chromatin has a longer nucleosome repeat than chicken liver chromatin, 212 base pairs as opposed to 200 base pairs (bp). More extensive digestion with micrococcal nuclease demonstrates that both tissues have 140 bp mucleosome cores. The difference in the length of the nucleosome repeat must therefore result from a difference in the length of the DNA linker between cores. Chicken erythrocyte chromatin is known to contain a tissue-specific, H1-like histone, H5, and to be genetically dormant. A model is presented to explain how changes in histone H1 could cause changes in the nucleosome repeat length and thereby alter the genetic activity of chromatin.
journal_name
Celljournal_title
Cellauthors
Morris NRdoi
10.1016/0092-8674(76)90045-3subject
Has Abstractpub_date
1976-12-01 00:00:00pages
627-32issue
4 Pt 1eissn
0092-8674issn
1097-4172pii
0092-8674(76)90045-3journal_volume
9pub_type
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