Abstract：
:We have identified a 2.4 kb partial cDNA clone (pDL34) for human von Willebrand factor (vWf) mRNA. pDL34 was selected by screening an endothelial cell cDNA library with a radiolabeled reverse transcript of mRNA obtained by specific immunoisolation of vWf polysomes from endothelial cells. pDL34 selectively hybridized to an endothelial cell-associated 9.5 kb mRNA. To confirm its identity, SP6 RNA polymerase was used to generate in vitro transcripts of the cDNA. This synthetic RNA, truncated at its 5' end, directed the synthesis of several unique polypeptides in rabbit reticulocyte lysates. These polypeptides were immunoprecipitated by polyclonal and monoclonal anti-vWf antibodies. These results indicate that pDL34 contains an authentic partial copy of vWf mRNA. In vitro transcription of partial cDNA clones and translation of the resulting RNAs may be a useful general method of verifying the identity of various cDNA clones in circumstances where antibodies are available and protein sequence is not.
journal_name
Celljournal_title
Cellauthors
Lynch DC,Zimmerman TS,Collins CJ,Brown M,Morin MJ,Ling EH,Livingston DMdoi
10.1016/0092-8674(85)90060-1subject
Has Abstractpub_date
1985-05-01 00:00:00pages
49-56issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(85)90060-1journal_volume
41pub_type
杂志文章相关文献
CELL文献大全abstract：:Nine transcripts complementary to mouse L cell mitochondrial DNA have been detected, sized and mapped to restriction fragments using the method of Berk and Sharp (1977). RNA isolated from L cell mitochondria was hybridized to 32P-labeled, cloned L cell mitochondrial DNA restriction fragments in 70% formamide under con...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(78)90309-4
更新日期：1978-05-01 00:00:00
abstract：:Aging has been associated with a progressive decline of proteostasis, but how this process affects proteome composition remains largely unexplored. Here, we profiled more than 5,000 proteins along the lifespan of the nematode C. elegans. We find that one-third of proteins change in abundance at least 2-fold during agi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.03.032
更新日期：2015-05-07 00:00:00
abstract：:The site on the HIV-1 gp120 glycoprotein that binds the CD4 receptor is recognized by broadly reactive antibodies, several of which neutralize over 90% of HIV-1 strains. To understand how antibodies achieve such neutralization, we isolated CD4-binding-site (CD4bs) antibodies and analyzed 16 co-crystal structures -8 de...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.05.007
更新日期：2015-06-04 00:00:00
abstract：:This year's Lasker-Bloomberg Public Service Award goes to GAVI, the Vaccine Alliance, for providing sustained access to childhood vaccines around the globe, saving millions of lives, and highlighting the power of immunization to prevent disease. ...
journal_title：Cell
pub_type： 历史文章,杂志文章
doi：10.1016/j.cell.2019.08.026
更新日期：2019-09-19 00:00:00
abstract：:We propose a mechanism for the priming of influenza viral RNA transcription by capped RNAs in which specific 5'-terminal fragments are cleaved from the capped RNAs by a virion-associated endonuclease. These fragments would serve as the actual primers for the initiation of transcription by the initial incorporation by ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90449-9
更新日期：1981-03-01 00:00:00
abstract：:This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90026-6
更新日期：1979-03-01 00:00:00
abstract：:We have examined the molecular organization of a region of mouse chromosome 17 that allows homologous recombination between wild-type and t haplotype chromosomes across a large inversion. We have used a combination of genetic mapping of restriction fragment length polymorphisms, molecular characterization of cloned re...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90078-x
更新日期：1987-03-13 00:00:00
abstract：:IFN-γ-activated macrophages attempt to limit pathogen growth through tryptophan starvation. Zhang et al. demonstrate that Mycobacterium tuberculosis responds to this threat by synthesizing its own tryptophan. Genetic and chemical inhibition of this pathway reduces mycobacterial survival, revealing a new immune-depende...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2013.11.015
更新日期：2013-12-05 00:00:00
abstract：:Immune responses in diverse tissue sites are critical for protective immunity and homeostasis. Here, we investigate how tissue localization regulates the development and function of human natural killer (NK) cells, innate lymphocytes important for anti-viral and tumor immunity. Integrating high-dimensional analysis of...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.01.022
更新日期：2020-02-20 00:00:00
abstract：:We have prepared polyclonal antibodies to the cytoplasmic portion of the envelope glycoprotein G of vesicular stomatitis virus (VSV) by using synthetic peptides corresponding to either the 22 or 11 ultimate carboxy-terminal residues of the G as immunogens. When antibodies to the 22 residue peptide are microinjected in...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90195-8
更新日期：1984-11-01 00:00:00
abstract：:Phosphodiesterases (PDEs) regulate the local concentration of 3',5' cyclic adenosine monophosphate (cAMP) within cells. cAMP activates the cAMP-dependent protein kinase (PKA). In patients, PDE inhibitors have been linked to heart failure and cardiac arrhythmias, although the mechanisms are not understood. We show that...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.07.030
更新日期：2005-10-07 00:00:00
abstract：:We have established the DNA sequence of nine yeast mutants that prevent the expression either of the split cytochrome b gene alone (five mutants) or of two split genes, the cytochrome b gene and the cytochrome oxidase subunit I gene (four mutants). All the mutations analyzed are localized in intron 14 of the cob-box g...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90051-4
更新日期：1982-04-01 00:00:00
abstract：:Mitochondrial Ca(2+) (Ca(2+)(m)) uptake is mediated by an inner membrane Ca(2+) channel called the uniporter. Ca(2+) uptake is driven by the considerable voltage present across the inner membrane (ΔΨ(m)) generated by proton pumping by the respiratory chain. Mitochondrial matrix Ca(2+) concentration is maintained five ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.10.011
更新日期：2012-10-26 00:00:00
abstract：:Thyroid hormone (T3) and retinoic acid (RA) receptors mediate ligand-dependent inhibition of epidermal growth factor (EGF) receptor and c-erbB2/neu promoter activities. Ligand-activated T3 and RA receptors act via a 36 bp 5' fragment of the EGF receptor gene in vivo and, in the presence of nuclear extract, bind with h...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90393-s
更新日期：1990-09-21 00:00:00
abstract：:FtsK acts at the bacterial division septum to couple chromosome segregation with cell division. We demonstrate that a truncated FtsK derivative, FtsK(50C), uses ATP hydrolysis to translocate along duplex DNA as a multimer in vitro, consistent with FtsK having an in vivo role in pumping DNA through the closing division...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(02)00624-4
更新日期：2002-01-25 00:00:00
abstract：:Epigenetics has recently evolved from a collection of diverse phenomena to a defined and far-reaching field of study. In this Essay, we examine the epistemology of epigenetics, provide a brief overview of underlying molecular mechanisms, and suggest future challenges for the field. ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2007.02.006
更新日期：2007-02-23 00:00:00
abstract：:In the mammalian brain, the specification of a single axon and multiple dendrites occurs early in the differentiation of most neuron types. Numerous intracellular signaling events for axon specification have been described in detail. However, the identity of the extracellular factor(s) that initiate neuronal polarity ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.06.010
更新日期：2010-07-09 00:00:00
abstract：:Egg laying by the nematode Caenorhabditis elegans requires the functioning of the vulva, the gonad, the egg-laying muscles, and the two HSN neurons, which innervate these muscles. By analyzing a newly isolated mutant (dig-1) that displaces the gonad, we discovered that cell interactions coordinate the spatial relation...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90382-o
更新日期：1990-09-21 00:00:00
abstract：:The chronic autoimmune diseases include multiple complex genetic disorders. Recently, genome-wide association studies (GWAS) have identified a large number of major loci, with many associations shared between various autoimmune diseases. These associations highlight key roles for lymphocyte activation and prioritize s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.03.003
更新日期：2010-03-19 00:00:00
abstract：:The precursor of the 55K adenovirus terminal protein is an 87K protein that is covalently linked to viral DNA. This protein is likely to be identical to the 80,000 dalton protein described by Challberg et al. (1980). The mRNA for the 87K terminal protein precursor, like that for the E2-72K DNA binding protein, is dete...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90145-8
更新日期：1981-02-01 00:00:00
abstract：:Posttranslational modifications of histones have been strongly correlated with transcriptional regulation. In this issue of Cell, comprehensively examined the nature of arginine methyltransferases and histone modifications in p53-mediated transcription. ...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2004.05.021
更新日期：2004-06-11 00:00:00
abstract：:A key function of blood vessels, to supply oxygen, is impaired in tumors because of abnormalities in their endothelial lining. PHD proteins serve as oxygen sensors and may regulate oxygen delivery. We therefore studied the role of endothelial PHD2 in vessel shaping by implanting tumors in PHD2(+/-) mice. Haplodeficien...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.01.020
更新日期：2009-03-06 00:00:00
abstract：:The central vasculature of plant roots is protected by a hydrophobic ring of endodermal cells that are enclosed by lamellae of suberin. Barberon et al. demonstrate that endodermal suberization plasticity facilitates ion homeostasis, with antithetical regulation of suberin deposition and degradation by the phytohormone...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2016.01.005
更新日期：2016-01-28 00:00:00
abstract：:The SIR2, SIR3, and SIR4 proteins are required for silencing of transcription at the silent mating type loci and at telomeres in yeast. Using protein affinity chromatography, we show that SIR2, SIR3, and two proteins of 69 and 110 kDa tightly associate with SIR4. Surprisingly, the 110 kDa SIR4-binding protein is ident...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80139-7
更新日期：1996-08-23 00:00:00
abstract：:Notch signaling regulates many aspects of metazoan development and tissue renewal. Accordingly, the misregulation or loss of Notch signaling underlies a wide range of human disorders, from developmental syndromes to adult-onset diseases and cancer. Notch signaling is remarkably robust in most tissues even though each ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2009.03.045
更新日期：2009-04-17 00:00:00
abstract：:A gene, bcl-3, is found on chromosome 19 adjacent to the breakpoints in the translocation t(14;19)(q32;q13.1), which occurs in some cases of chronic lymphocytic leukemia. Sequence analysis of the human bcl-3 gene predicts a protein containing seven tandem copies of the SWI6/cdc10 motif. This motif was previously ident...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90347-h
更新日期：1990-03-23 00:00:00
abstract：:Senescence in Podospora anserina has long been shown to be under cytoplasmic control. Comparison of DNAs isolated from young and senescent cultures made it possible to detect the presence, in senescent cultures only, of a specific DNA (SEN-DNA). This DNA consists of repeated sequences arranged in a multimeric set of c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(80)90126-9
更新日期：1980-08-01 00:00:00
abstract：:The retinoblastoma tumor suppressor protein (RB) binds several cellular proteins involved in cell cycle progression. Using the yeast two-hybrid system, we found that RB bound specifically to the protein BRG1. BRG1 shares extensive sequence similarity to Drosophila brahma, an activator of homeotic gene expression, and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90405-7
更新日期：1994-10-07 00:00:00
abstract：:We are undertaking a genetic approach to investigate the role that synaptic modulation in the mammalian central nervous system plays in learning and memory and to identify relevant molecular components. We have generated mice deficient in the gamma isoform of protein kinase C (PKC gamma), an enzyme that has previously...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90614-v
更新日期：1993-12-31 00:00:00
abstract：:Through dissecting the link between spatial genome organization and DNA replication timing, Sima et al. (2018) discover early replicating control elements (ERCEs), a new type of cis-acting elements that regulate replication timing, transcription, and multiple layers of three-dimensional features of genome organization...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2019.01.027
更新日期：2019-02-07 00:00:00
