Hematopoiesis: an evolving paradigm for stem cell biology.

abstract：:Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C∗ complex) at an average resolution of 3.76 Å. The splicing factor Prp17...

journal_title：Cell

authors： Zhang X,Yan C,Hang J,Finci LI,Lei J,Shi Y

更新日期：2017-05-18 00:00:00

abstract：:The yeast plasmid 2 micron circle actively maintains high but stable copy levels in the cell, even though the plasmid confers no selective advantage to its host. To address the mechanism by which stable copy control is achieved, we have examined the level of expression of the genes resident on the yeast plasmid 2 micr...

journal_title：Cell

authors： Som T,Armstrong KA,Volkert FC,Broach JR

更新日期：1988-01-15 00:00:00

abstract：:Micronuclei of Tetrahymena thermophila contain two electrophoretically distinct forms of histone H3. The slower migrating micronuclear species, H3S, is indistinguishable from the macronuclear H3 by electrophoretic analyses in three gel systems and by partial proteolytic peptide mapping. The faster species, H3F, is uni...

journal_title：Cell

authors： Allis CD,Bowen JK,Abraham GN,Glover CV,Gorovsky MA

更新日期：1980-05-01 00:00:00

abstract：:The Bcl-2 family proteins are key regulators of apoptosis in human diseases and cancers. Though known to block apoptosis, Bcl-2 promotes cell death through an undefined mechanism. Here, we show that Bcl-2 interacts with orphan nuclear receptor Nur77 (also known as TR3), which is required for cancer cell apoptosis indu...

journal_title：Cell

authors： Lin B,Kolluri SK,Lin F,Liu W,Han YH,Cao X,Dawson MI,Reed JC,Zhang XK

更新日期：2004-02-20 00:00:00

abstract：:Liddle's syndrome is an inherited form of hypertension caused by mutations that truncate the C-terminus of human epithelial Na+ channel (hENaC) subunits. Expression of truncated beta and gamma hENaC subunits increased Na+ current. However, truncation did not alter single-channel conductance or open state probability, ...

journal_title：Cell

authors： Snyder PM,Price MP,McDonald FJ,Adams CM,Volk KA,Zeiher BG,Stokes JB,Welsh MJ

更新日期：1995-12-15 00:00:00

abstract：:Rubisco, the key enzyme of CO2 fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis...

journal_title：Cell

authors： Flecken M,Wang H,Popilka L,Hartl FU,Bracher A,Hayer-Hartl M

更新日期：2020-10-15 00:00:00

abstract：:The mechanisms of transcriptional activation in heterochromatin were investigated by using FISH to directly visualize changes in chromatin organization during activation of a heterochromatic lambda5 transgene. A DNase I hypersensitive site was shown to relocate the transgene to the outside of the pericentromeric heter...

journal_title：Cell

authors： Lundgren M,Chow CM,Sabbattini P,Georgiou A,Minaee S,Dillon N

更新日期：2000-11-22 00:00:00

abstract：:The S. cerevisiae gene MCR1 encodes two mitochondrial isoforms of NADH-cytochrome b5 reductase. The primary translation product has an amino-terminal matrix-targeting signal, followed by a stretch of 21 uncharged amino acids. This precursor protein is inserted into the outer membrane, but only about one-third of the m...

journal_title：Cell

authors： Hahne K,Haucke V,Ramage L,Schatz G

更新日期：1994-12-02 00:00:00

abstract：:Clonal derivatives of C3HMT murine mammary cell lines in culture demonstrate conversion of mammary tumor virus (MMTV) expression at a rate of appriximately 6 per 100 clones. This alteration is largely unidirectional from a relatively high level (MMTV(H)) to a 10 fold lower level (MMTV(L)). This high rate of MMTV(L) va...

journal_title：Cell

authors： Parks WP,Hubbell ES,Goldberg RJ,O'Neill FJ,Scolnick EM

更新日期：1976-05-01 00:00:00

abstract：:We have identified an essential gene, called FIP1, encoding a 327 amino acid protein interacting with yeast poly(A) polymerase (PAP1) in the two-hybrid assay. Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro. At 37 degrees C, a thermosensitive allele of FIP1 shows a shortening of poly(A) tails and a dec...

journal_title：Cell

authors： Preker PJ,Lingner J,Minvielle-Sebastia L,Keller W

更新日期：1995-05-05 00:00:00

abstract：:We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This "Q system" offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expre...

journal_title：Cell

authors： Potter CJ,Tasic B,Russler EV,Liang L,Luo L

更新日期：2010-04-30 00:00:00

abstract：:Nociceptin and its receptor are widely distributed throughout the brain in regions associated with reward behavior, yet how and when they act is unknown. Here, we dissected the role of a nociceptin peptide circuit in reward seeking. We generated a prepronociceptin (Pnoc)-Cre mouse line that revealed a unique subpopula...

journal_title：Cell

authors： Parker KE,Pedersen CE,Gomez AM,Spangler SM,Walicki MC,Feng SY,Stewart SL,Otis JM,Al-Hasani R,McCall JG,Sakers K,Bhatti DL,Copits BA,Gereau RW,Jhou T,Kash TJ,Dougherty JD,Stuber GD,Bruchas MR

更新日期：2019-07-25 00:00:00

abstract：:Leukocyte adhesion deficiency (LAD) is a heritable disease involving deficient expression of three related leukocyte adhesion glycoproteins: LFA-1, Mac-1, and p150,95. These proteins are alpha beta heterodimers containing identical 95,000 dalton beta subunits. Here we demonstrate that the primary defect in LAD is in t...

journal_title：Cell

authors： Kishimoto TK,Hollander N,Roberts TM,Anderson DC,Springer TA

更新日期：1987-07-17 00:00:00

abstract：:The HIV-1 matrix (MA) protein contains two subcellular localization signals with opposing effects. A myristoylated N-terminus governs particle assembly at the plasma membrane, and a nucleophilic motif facilitates import of the viral preintegration complex into the nucleus of nondividing cells. Here, we show that myris...

journal_title：Cell

authors： Gallay P,Swingler S,Aiken C,Trono D

更新日期：1995-02-10 00:00:00

abstract：:Thrombin stimulates 32Pi incorporation into phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol 4,5-bis-phosphate (PIP2), and phosphatidylinositol (PI), and initiates DNA synthesis in hamster (NIL) fibroblasts at a half-maximal concentration of 125 ng/ml. Neomycin, which binds PIP2 and PIP, inhibits both thro...

journal_title：Cell

authors： Carney DH,Scott DL,Gordon EA,LaBelle EF

更新日期：1985-09-01 00:00:00

abstract：:The basal ganglia (BG) are critical for adaptive motor control, but the circuit principles underlying their pathway-specific modulation of target regions are not well understood. Here, we dissect the mechanisms underlying BG direct and indirect pathway-mediated control of the mesencephalic locomotor region (MLR), a br...

journal_title：Cell

authors： Roseberry TK,Lee AM,Lalive AL,Wilbrecht L,Bonci A,Kreitzer AC

更新日期：2016-01-28 00:00:00

abstract：:The leader RNA transcript of vesicular stomatitis virus (VSV) has been immunoprecipitated from infected BHK cell extracts by anti-La specific sera from patients with systemic lupus erythematosus (SLE). This association was specific as lupus anti-sera with other specificities failed to precipitate leader RNA. The amoun...

journal_title：Cell

authors： Kurilla MG,Keene JD

更新日期：1983-10-01 00:00:00

abstract：:It has been proposed that the initiator protein RepA is rate limiting for mini-P1 plasmid replication, and that the role of the plasmid copy number control locus is to sequester the initiator and thus reduce replication. This proposal appears inconsistent with the observation that RepA is autoregulated, since the prot...

journal_title：Cell

authors： Chattoraj DK,Mason RJ,Wickner SH

更新日期：1988-02-26 00:00:00

abstract：:Dietary restriction (DR) extends lifespan in multiple species. To examine the mechanisms of lifespan extension upon DR, we assayed genome-wide translational changes in Drosophila. A number of nuclear encoded mitochondrial genes, including those in Complex I and IV of the electron transport chain, showed increased ribo...

journal_title：Cell

authors： Zid BM,Rogers AN,Katewa SD,Vargas MA,Kolipinski MC,Lu TA,Benzer S,Kapahi P

更新日期：2009-10-02 00:00:00

abstract：:The global rise in the prevalence of obesity and associated co-morbidities such as type 2 diabetes, cardiovascular disease, and cancer represents a major public health concern. The biological response to increased consumption of palatable foods or a reduction in energy expenditure is highly variable between individual...

journal_title：Cell

authors： van der Klaauw AA,Farooqi IS

更新日期：2015-03-26 00:00:00

abstract：:The dual host range virus HIX has been previously characterized as an envelope gene recombinant between Moloney murine leukemia virus (Mo-MuLV) and an unidentified xenotropic murine leukemia virus. Using long reverse transcripts of Mo-MuLV, a region of nonhomology has been mapped by electron microscopic analysis of he...

journal_title：Cell

authors： Donoghue DJ,Rothenberg E,Hopkins N,Baltimore D,Sharp PA

更新日期：1978-08-01 00:00:00

abstract：:Chemical modifications of histones and DNA, such as histone methylation, histone acetylation, and DNA methylation, play critical roles in epigenetic gene regulation. Many of the enzymes that add or remove such chemical modifications are known, or might be suspected, to be sensitive to changes in intracellular metaboli...

journal_title：Cell

authors： Kaelin WG Jr,McKnight SL

更新日期：2013-03-28 00:00:00

abstract：:The global financial crisis has hit biotech companies hard both in the US and Europe as venture capital dries up. Finding new sources of long-term financing for translating research into new therapeutics will be essential for maintaining innovation and new drug development by biotech companies. ...

journal_title：Cell

authors： Karberg S

更新日期：2009-08-07 00:00:00

abstract：:The fovea is a specialized region of the retina that dominates the visual perception of primates by providing high chromatic and spatial acuity. While the foveal and peripheral retina share a similar core circuit architecture, they exhibit profound functional differences whose mechanisms are unknown. Using intracellul...

journal_title：Cell

authors： Sinha R,Hoon M,Baudin J,Okawa H,Wong ROL,Rieke F

更新日期：2017-01-26 00:00:00

abstract：:Transfer RNA (tRNA) gene content is a differentiating feature of genomes that contributes to the efficiency of the translational apparatus, but the principles shaping tRNA gene copy number and codon composition are poorly understood. Here, we report that the emergence of two specific tRNA modifications shaped the stru...

journal_title：Cell

authors： Novoa EM,Pavon-Eternod M,Pan T,Ribas de Pouplana L

更新日期：2012-03-30 00:00:00

abstract：:Study of Asians has previously indicated that deletion of alpha-globin structural genes is the predominant lesion in alpha-thalassemias and that Hb H disease occurs when three of four normal alpha loci per cell are deleted. To test the generality of this model, Hb H disease DNAs of both Asian and non-Asian origin were...

journal_title：Cell

authors： Orkin SH,Old J,Lazarus H,Altay C,Gurgey A,Weatherall DJ,Nathan DG

更新日期：1979-05-01 00:00:00

abstract：:Reversible S-palmitoylation of cysteine residues critically controls transient membrane tethering of peripheral membrane proteins. Little is known about how the palmitoylation machinery governs their defined localization and function. We monitored the spatially resolved reaction dynamics and substrate specificity of t...

journal_title：Cell

authors： Rocks O,Gerauer M,Vartak N,Koch S,Huang ZP,Pechlivanis M,Kuhlmann J,Brunsveld L,Chandra A,Ellinger B,Waldmann H,Bastiaens PI

更新日期：2010-04-30 00:00:00

abstract：:Wnt signaling plays a critical role in embryonic development, and genetic aberrations in this network have been broadly implicated in colorectal cancer. We find that the Wnt receptor Frizzled2 (Fzd2) and its ligands Wnt5a/b are elevated in metastatic liver, lung, colon, and breast cancer cell lines and in high-grade t...

journal_title：Cell

authors： Gujral TS,Chan M,Peshkin L,Sorger PK,Kirschner MW,MacBeath G

更新日期：2014-11-06 00:00:00

abstract：:Adenovirus E1B 19 kDa protein protects against cell death induced by viral infection and certain external stimuli. The Bcl-2 protein can functionally substitute for the E1B 19 kDa protein. To identify cellular targets for the 19 kDa protein, we used the two-hybrid screen in yeast. We have isolated cDNAs for three diff...

journal_title：Cell

authors： Boyd JM,Malstrom S,Subramanian T,Venkatesh LK,Schaeper U,Elangovan B,D'Sa-Eipper C,Chinnadurai G

更新日期：1994-10-21 00:00:00

abstract：:The intestinal microbiota produces tens of thousands of metabolites. Here, we used host sensing of small molecules by G-protein coupled receptors (GPCRs) as a lens to illuminate bioactive microbial metabolites that impact host physiology. We screened 144 human gut bacteria against the non-olfactory GPCRome and identif...

journal_title：Cell

authors： Chen H,Nwe PK,Yang Y,Rosen CE,Bielecka AA,Kuchroo M,Cline GW,Kruse AC,Ring AM,Crawford JM,Palm NW

更新日期：2019-05-16 00:00:00