The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis.

abstract：:Type 2 diabetes (T2D) is a worldwide epidemic with a medical need for additional targeted therapies. Suppression of hepatic glucose production (HGP) effectively ameliorates diabetes and can be exploited for its treatment. We hypothesized that targeting PGC-1α acetylation in the liver, a chemical modification known to ...

journal_title：Cell

authors： Sharabi K,Lin H,Tavares CDJ,Dominy JE,Camporez JP,Perry RJ,Schilling R,Rines AK,Lee J,Hickey M,Bennion M,Palmer M,Nag PP,Bittker JA,Perez J,Jedrychowski MP,Ozcan U,Gygi SP,Kamenecka TM,Shulman GI,Schreiber SL,Gr

更新日期：2017-03-23 00:00:00

abstract：:Human autoantibodies that recognize the spindle poles of mammals, plants, and insects were found to recognize two antigens in yeast. One of these proteins, called SPA1 (for Spindle Pole Antigen), is antigenically related to the spindle poles of a diverse set of organisms. The gene encoding SPA1 was cloned by immunoscr...

journal_title：Cell

authors： Snyder M,Davis RW

更新日期：1988-09-09 00:00:00

abstract：:Study of Asians has previously indicated that deletion of alpha-globin structural genes is the predominant lesion in alpha-thalassemias and that Hb H disease occurs when three of four normal alpha loci per cell are deleted. To test the generality of this model, Hb H disease DNAs of both Asian and non-Asian origin were...

journal_title：Cell

authors： Orkin SH,Old J,Lazarus H,Altay C,Gurgey A,Weatherall DJ,Nathan DG

更新日期：1979-05-01 00:00:00

abstract：:Transcription of U1 small nuclear RNA from a 592 bp fragment of human DNA was analyzed in vivo and in vitro. When injected into Xenopus laevis oocyte nuclei, the cloned DNA is transcribed by RNA polymerase II to make human U1 snRNA. Thus the sequences of this fragment are sufficient for expression of the U1 snRNA gene...

journal_title：Cell

authors： Murphy JT,Burgess RR,Dahlberg JE,Lund E

更新日期：1982-05-01 00:00:00

abstract：:There are still no effective treatments to prevent, halt, or reverse Alzheimer's disease, but research advances over the past three decades could change this gloomy picture. Genetic studies demonstrate that the disease has multiple causes. Interdisciplinary approaches combining biochemistry, molecular and cell biology...

journal_title：Cell

authors： Huang Y,Mucke L

更新日期：2012-03-16 00:00:00

abstract：:Cyclic dinucleotides (CDNs) have been previously recognized as important secondary signaling molecules in bacteria and, more recently, in mammalian cells. In the former case, they represent secondary messengers affecting numerous responses of the prokaryotic cell, whereas in the latter, they act as agonists of the inn...

journal_title：Cell

authors： Danilchanka O,Mekalanos JJ

更新日期：2013-08-29 00:00:00

abstract：:K-Ras is targeted to the plasma membrane by a C-terminal membrane anchor that comprises a farnesyl-cysteine-methyl-ester and a polybasic domain. We used quantitative spatial imaging and atomistic molecular dynamics simulations to examine molecular details of K-Ras plasma membrane binding. We found that the K-Ras ancho...

journal_title：Cell

authors： Zhou Y,Prakash P,Liang H,Cho KJ,Gorfe AA,Hancock JF

更新日期：2017-01-12 00:00:00

abstract：:The precise control of miR-17∼92 microRNA (miRNA) is essential for normal development, and overexpression of certain miRNAs from this cluster is oncogenic. Here, we find that the relative expression of the six miRNAs processed from the primary (pri-miR-17∼92) transcript is dynamically regulated during embryonic stem c...

journal_title：Cell

authors： Du P,Wang L,Sliz P,Gregory RI

更新日期：2015-08-13 00:00:00

abstract：:Sir2 is a conserved deacetylase that modulates life span in yeast, worms, and flies and stress response in mammals. In yeast, Sir2 is required for maintaining replicative life span, and increasing Sir2 dosage can delay replicative aging. We address the role of Sir2 in regulating chronological life span in yeast. Lack ...

journal_title：Cell

authors： Fabrizio P,Gattazzo C,Battistella L,Wei M,Cheng C,McGrew K,Longo VD

更新日期：2005-11-18 00:00:00

abstract：:The lateral hypothalamic (LH) projection to the ventral tegmental area (VTA) has been linked to reward processing, but the computations within the LH-VTA loop that give rise to specific aspects of behavior have been difficult to isolate. We show that LH-VTA neurons encode the learned action of seeking a reward, indepe...

journal_title：Cell

authors： Nieh EH,Matthews GA,Allsop SA,Presbrey KN,Leppla CA,Wichmann R,Neve R,Wildes CP,Tye KM

更新日期：2015-01-29 00:00:00

abstract：:We have developed a cell-free system for studying the integration of retroviral DNA. In our assay, amber mutations in a bacteriophage lambda genome that serves as the target for integration are suppressed by integration of an MLV derivative that carries the E. coli supF gene. The structure of the reaction products is ...

journal_title：Cell

authors： Brown PO,Bowerman B,Varmus HE,Bishop JM

更新日期：1987-05-08 00:00:00

abstract：:Advances in genomic technology have produced an explosion of new information about the genetic basis for human disease, fueling extraordinarily high expectations for improved treatments. This perspective will take brief stock of what genetics/genomics have brought to clinical practice to date and what we might expect ...

journal_title：Cell

authors： Ginsburg D

更新日期：2011-09-30 00:00:00

abstract：:PTEN is a frequently mutated tumor suppressor gene that opposes the PI3K/AKT pathway through dephosphorylation of phosphoinositide-3,4,5-triphosphate. Recently, nuclear compartmentalization of PTEN was found as a key component of its tumor-suppressive activity; however its nuclear function remains poorly defined. Here...

journal_title：Cell

authors： Song MS,Carracedo A,Salmena L,Song SJ,Egia A,Malumbres M,Pandolfi PP

更新日期：2011-01-21 00:00:00

abstract：:Genes are packaged into nucleosomal arrays, each nucleosome typically having two copies of histones H2A, H2B, H3, and H4. Histones have distinct posttranslational modifications, variant isoforms, and dynamics. Whether each histone copy within a nucleosome has distinct properties, particularly in relation to the direct...

journal_title：Cell

authors： Rhee HS,Bataille AR,Zhang L,Pugh BF

更新日期：2014-12-04 00:00:00

abstract：:The Eph receptor tyrosine kinase family is regulated by autophosphorylation within the juxtamembrane region and the kinase activation segment. We have solved the X-ray crystal structure to 1.9 A resolution of an autoinhibited, unphosphorylated form of EphB2 comprised of the juxtamembrane region and the kinase domain. ...

journal_title：Cell

authors： Wybenga-Groot LE,Baskin B,Ong SH,Tong J,Pawson T,Sicheri F

更新日期：2001-09-21 00:00:00

abstract：:The oligosaccharide-lipid which is the precursor of asparagine-linked oligosaccharides of eucaryotic glycoproteins is synthesized from sugar nucleotides in the endoplasmic reticulum. The transmembrane location of the assembly of this oligosaccharide-lipid has been studied in vitro in rat liver microsomes. Protease tre...

journal_title：Cell

authors： Snider MD,Sultzman LA,Robbins PW

更新日期：1980-09-01 00:00:00

abstract：:An acute but transient response to insulin is essential for glucose homeostasis in mammals. Chakraborty et al. (2010) uncover a new feedback mechanism regulating insulin signaling. They show that the inositol pyrophosphate IP7, which is produced in response to insulin, inhibits the Akt kinase, a primary effector of in...

journal_title：Cell

authors： Manning BD

更新日期：2010-12-10 00:00:00

abstract：:Metastasis is a rare event. Does it arise from rare, variant, highly metastatic cells or does a primary tumor progress to a premalignant state from which metastases arise stochastically without further changes in gene expression? Arguments and evidence have been adduced to support either position. A paper in this mont...

journal_title：Cell

authors： Hynes RO

更新日期：2003-06-27 00:00:00

abstract：:Recessive lethal mutations in the T/t-complex of the mouse characteristically show defective genetic complementation, even when they affect very different stages of embryogenesis and are known to be nonallelic. To address the question of their genetic or functional relationship, we have applied the cis-trans test, usi...

journal_title：Cell

authors： Shin HS,McCormick P,Artzt K,Bennett D

更新日期：1983-07-01 00:00:00

abstract：:Radioactive alpha factor is degraded to discrete biologically inactive fragments by the target a cells of S. cerevisiae, but not by alpha cells which make the pheromone. The pattern of cleavage products and sequence analysis of one fragment indicated that the first scission occurred between leucine 6 and lysine 7. The...

journal_title：Cell

authors： Ciejek E,Thorner J

更新日期：1979-11-01 00:00:00

abstract：:The stomata of plants regulate gas exchange and water transpiration in response to changing environmental conditions. New work reveals that stomata also have an important role in host defense. In this issue of Cell, Melotto et al. (2006) show that stomata close upon detection of potential microbial pathogens to preven...

journal_title：Cell

authors： Schulze-Lefert P,Robatzek S

更新日期：2006-09-08 00:00:00

abstract：:The low-complexity (LC) domains of the products of the fused in sarcoma (FUS), Ewings sarcoma (EWS), and TAF15 genes are translocated onto a variety of different DNA-binding domains and thereby assist in driving the formation of cancerous cells. In the context of the translocated fusion proteins, these LC sequences fu...

journal_title：Cell

authors： Kwon I,Kato M,Xiang S,Wu L,Theodoropoulos P,Mirzaei H,Han T,Xie S,Corden JL,McKnight SL

更新日期：2013-11-21 00:00:00

abstract：:X-linked lissencephaly and "double cortex" are allelic human disorders mapping to Xq22.3-Xq23 associated with arrest of migrating cerebral cortical neurons. We identified a novel 10 kb brain-specific cDNA interrupted by a balanced translocation in an XLIS patient that encodes a novel 40 kDa predicted protein named Dou...

journal_title：Cell

authors： Gleeson JG,Allen KM,Fox JW,Lamperti ED,Berkovic S,Scheffer I,Cooper EC,Dobyns WB,Minnerath SR,Ross ME,Walsh CA

更新日期：1998-01-09 00:00:00

abstract：:In eukaryotes, a specialized pathway of mRNA degradation termed nonsense-mediated decay (NMD) functions in mRNA quality control by recognizing and degrading mRNAs with aberrant termination codons. We demonstrate that NMD in yeast targets premature termination codon (PTC)-containing mRNA to P-bodies. Upf1p is sufficien...

journal_title：Cell

authors： Sheth U,Parker R

更新日期：2006-06-16 00:00:00

abstract：:The Ion gene of E. coli controls the stability of two bacteriophage lambda proteins. The functional half-life of the phage N gene product, measured by complementation, is increased about 5-fold in Ion mutant strains, from 2 min to 10 min. The chemical half-life of N protein, determined by its disappearance on polyacry...

journal_title：Cell

authors： Gottesman S,Gottesman M,Shaw JE,Pearson ML

更新日期：1981-04-01 00:00:00

abstract：:Behavioral expression of food-associated memory in fruit flies is constrained by satiety and promoted by hunger, suggesting an influence of motivational state. Here, we identify a neural mechanism that integrates the internal state of hunger and appetitive memory. We show that stimulation of neurons that express neuro...

journal_title：Cell

authors： Krashes MJ,DasGupta S,Vreede A,White B,Armstrong JD,Waddell S

更新日期：2009-10-16 00:00:00

abstract：:32P-labeled cowpea mosaic virus (CPMV) RNAs were analyzed for their 5' termini by three different fingerprinting standard procedures. These analyses showed unequivocally that CPMV RNAs lack a structure like m7GpppN ... at their 5' termini. In addition, neither mono-, di- or triphosphorylated [(p)(p)pN ....] termini we...

journal_title：Cell

authors： Klootwijk J,Klein I,Zabel P,van Kammen A

更新日期：1977-05-01 00:00:00

abstract：:Smad4 is an important mediator of signaling by TGFbeta family members that is presumed to be controlled by the phosphorylation status of its partners, the receptor-regulated R-Smads. In this issue, Dupont et al. (2009) now reveal that Smad4 itself is subject to cycles of ubiquitination and deubiquitination that regula...

journal_title：Cell

authors： Wrana JL

更新日期：2009-01-09 00:00:00

abstract：:The exchange of metabolites between the mitochondrial matrix and the cytosol depends on β-barrel channels in the outer membrane and α-helical carrier proteins in the inner membrane. The essential translocase of the inner membrane (TIM) chaperones escort these proteins through the intermembrane space, but the structura...

journal_title：Cell

authors： Weinhäupl K,Lindau C,Hessel A,Wang Y,Schütze C,Jores T,Melchionda L,Schönfisch B,Kalbacher H,Bersch B,Rapaport D,Brennich M,Lindorff-Larsen K,Wiedemann N,Schanda P

更新日期：2018-11-15 00:00:00

abstract：:A functional cDNA from Aspergillus nidulans encoding triosephosphate isomerase (TPI) was isolated by its ability to complement a tpi1 mutation in Saccharomyces cerevisiae. This cDNA was used to obtain the corresponding gene, tpiA. Alignment of the cDNA and genomic DNA nucleotide sequences indicated that tpiA contains ...

journal_title：Cell

authors： McKnight GL,O'Hara PJ,Parker ML

更新日期：1986-07-04 00:00:00