Abstract:
:A functional cDNA from Aspergillus nidulans encoding triosephosphate isomerase (TPI) was isolated by its ability to complement a tpi1 mutation in Saccharomyces cerevisiae. This cDNA was used to obtain the corresponding gene, tpiA. Alignment of the cDNA and genomic DNA nucleotide sequences indicated that tpiA contains five introns. The intron positions in the tpiA gene were compared with those in the TPI genes of human, chicken, and maize. One intron is present at an identical position in all four organisms, two other introns are located in similar positions in A. nidulans and maize, and the remaining two introns are unique to A. nidulans. These Aspergillus-specific introns are located in regions of the protein that were predicted to be interrupted by introns based on analysis of a Go plot of chicken TPI. These comparisons are discussed in relation to the evolution of introns within TPI genes.
journal_name
Celljournal_title
Cellauthors
McKnight GL,O'Hara PJ,Parker MLdoi
10.1016/0092-8674(86)90868-8subject
Has Abstractpub_date
1986-07-04 00:00:00pages
143-7issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(86)90868-8journal_volume
46pub_type
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