Nucleotide sequence of the triosephosphate isomerase gene from Aspergillus nidulans: implications for a differential loss of introns.


:A functional cDNA from Aspergillus nidulans encoding triosephosphate isomerase (TPI) was isolated by its ability to complement a tpi1 mutation in Saccharomyces cerevisiae. This cDNA was used to obtain the corresponding gene, tpiA. Alignment of the cDNA and genomic DNA nucleotide sequences indicated that tpiA contains five introns. The intron positions in the tpiA gene were compared with those in the TPI genes of human, chicken, and maize. One intron is present at an identical position in all four organisms, two other introns are located in similar positions in A. nidulans and maize, and the remaining two introns are unique to A. nidulans. These Aspergillus-specific introns are located in regions of the protein that were predicted to be interrupted by introns based on analysis of a Go plot of chicken TPI. These comparisons are discussed in relation to the evolution of introns within TPI genes.






McKnight GL,O'Hara PJ,Parker ML




Has Abstract


1986-07-04 00:00:00
















  • RNA-mediated recombination in S. cerevisiae.

    abstract::The existence of pseudogenes and the observation of intron loss suggest that RNA can serve as an intermediate in recombination. We used a HIS3 reporter gene to show that RNA-mediated recombination occurs in yeast. His3+ prototroph formation required transcription and expression of the retrotransposon Ty. Two RNA-media...


    pub_type: 杂志文章


    authors: Derr LK,Strathern JN,Garfinkel DJ

    更新日期:1991-10-18 00:00:00

  • Metabolic Instruction of Immunity.

    abstract::Choices have consequences. Immune cells survey and migrate throughout the body and sometimes take residence in niche environments with distinct communities of cells, extracellular matrix, and nutrients that may differ from those in which they matured. Imbedded in immune cell physiology are metabolic pathways and metab...


    pub_type: 杂志文章,评审


    authors: Buck MD,Sowell RT,Kaech SM,Pearce EL

    更新日期:2017-05-04 00:00:00

  • Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development.

    abstract::The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die at embryonic day 16.5. Their phenotype includes severe craniofac...


    pub_type: 杂志文章


    authors: Cecconi F,Alvarez-Bolado G,Meyer BI,Roth KA,Gruss P

    更新日期:1998-09-18 00:00:00

  • Neuronal Computation Underlying Inferential Reasoning in Humans and Mice.

    abstract::Every day we make decisions critical for adaptation and survival. We repeat actions with known consequences. But we also draw on loosely related events to infer and imagine the outcome of entirely novel choices. These inferential decisions are thought to engage a number of brain regions; however, the underlying neuron...


    pub_type: 杂志文章


    authors: Barron HC,Reeve HM,Koolschijn RS,Perestenko PV,Shpektor A,Nili H,Rothaermel R,Campo-Urriza N,O'Reilly JX,Bannerman DM,Behrens TEJ,Dupret D

    更新日期:2020-10-01 00:00:00

  • Maternally transmitted resistance to lymphoma development in mice of reciprocal crosses of the RF/J and AKR/J strains.

    abstract::Females but not males of the low-lymphoma RF/J strain transmit a non-Mendelian factor which suppresses the development of lymphoma in F1 crosses with mice of the high-lymphoma AKR/J strain. Suppression of lymphoma was also evident in the first backcross generation to the parental AKR strain, but only when (RF female x...


    pub_type: 杂志文章


    authors: Mayer A,Struuck FD,Duran-Reynals ML,Lilly F

    更新日期:1980-02-01 00:00:00

  • Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments.

    abstract::An easy manipulation of the commercial fluorescence microscope allows stereo pairs of pictures to be taken, which when examined with a stereo viewer, give a strong three-dimensional impression. The procedure is described in detail. Its use allows the documentation by immunofluorescence microscopy of the three-dimensio...


    pub_type: 杂志文章


    authors: Osborn M,Born T,Koitsch HJ,Weber K

    更新日期:1978-07-01 00:00:00

  • Derepression of a neuronal inhibitor due to miRNA dysregulation in a schizophrenia-related microdeletion.

    abstract::22q11.2 microdeletions result in specific cognitive deficits and schizophrenia. Analysis of Df(16)A(+/-) mice, which model this microdeletion, revealed abnormalities in the formation of neuronal dendrites and spines, as well as altered brain microRNAs. Here, we show a drastic reduction of miR-185, which resides within...


    pub_type: 杂志文章


    authors: Xu B,Hsu PK,Stark KL,Karayiorgou M,Gogos JA

    更新日期:2013-01-17 00:00:00

  • Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens.

    abstract::The sequence of about one third of the polyoma virus genome is presented. This sequence covers the origin of replication of two large plaque strains (A2 and A3) of polyoma virus. The two strains differ by 11 bp in the origin region. A model for replication is suggested. The sequence probably also covers the entire cod...


    pub_type: 杂志文章


    authors: Soeda E,Arrand JR,Smolar N,Griffin BE

    更新日期:1979-06-01 00:00:00

  • Progression to steroid insensitivity can occur irrespective of the presence of functional steroid receptors.

    abstract::A major problem in treatment of cancers arising in steroid-sensitive cells is their inevitable progression to a steroid-insensitive state; current therapies are based on the assumption that hormone insensitivity is associated with loss of receptor. We demonstrate for the first time that breast tumor cells can progress...


    pub_type: 杂志文章


    authors: Darbre PD,King RJ

    更新日期:1987-11-20 00:00:00

  • No-nonsense functions for long noncoding RNAs.

    abstract::The mysterious secrets of long noncoding RNAs, often referred to as the Dark Matter of the genome, are gradually coming to light. Several recent papers dig deep to reveal surprisingly complex and diverse functions of these enigmatic molecules. ...


    pub_type: 杂志文章,评审


    authors: Nagano T,Fraser P

    更新日期:2011-04-15 00:00:00

  • Reviving the exosome.

    abstract::In the nucleus of eukaryotic cells, RNA decay in the 3'-5' direction is carried out by a complex of exonucleases called the exosome. Surprisingly, the purified exosome shows only weak activity in vitro. Two papers in this issue of Cell, by LaCava et al. (2005) and Wyers et al. (2005), and a third report by Vanacova et...


    pub_type: 评论,杂志文章


    authors: Jensen TH,Moore C

    更新日期:2005-06-03 00:00:00

  • The structures and fidelity of replication of mouse mitochondrial DNA-pSC 101 EcoRI recombinant plasmids grown in E. coli K12.

    abstract::Recombinant DNAs containing the E. coli plasmid pSC101 and mouse cell (La9) mitochondrial DNA (mtDNA) were formed in vitro via ligation of DNA fragments from limit EcoRI endonuclease digests and were used to transform E. coli K12. Four structurally different recombinant plasmid DNAs from transformed clones were charac...


    pub_type: 杂志文章


    authors: Brown WM,Watson RM,Vinograd J,Tait KM,Boyer HW,Goodman HM

    更新日期:1976-04-01 00:00:00

  • An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.

    abstract::Characterizing cell surface receptors mediating viral infection is critical for understanding viral tropism and developing antiviral therapies. Nevertheless, due to challenges associated with detecting protein interactions on the cell surface, the host receptors of many human pathogens remain unknown. Here, we build a...


    pub_type: 杂志文章


    authors: Martinez-Martin N,Marcandalli J,Huang CS,Arthur CP,Perotti M,Foglierini M,Ho H,Dosey AM,Shriver S,Payandeh J,Leitner A,Lanzavecchia A,Perez L,Ciferri C

    更新日期:2018-08-23 00:00:00

  • Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.

    abstract::Bacteria acquire memory of viral invaders by incorporating invasive DNA sequence elements into the host CRISPR locus, generating a new spacer within the CRISPR array. We report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion i...


    pub_type: 杂志文章


    authors: Wang J,Li J,Zhao H,Sheng G,Wang M,Yin M,Wang Y

    更新日期:2015-11-05 00:00:00

  • Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses.

    abstract::To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell ...


    pub_type: 杂志文章


    authors: Lavin Y,Kobayashi S,Leader A,Amir ED,Elefant N,Bigenwald C,Remark R,Sweeney R,Becker CD,Levine JH,Meinhof K,Chow A,Kim-Shulze S,Wolf A,Medaglia C,Li H,Rytlewski JA,Emerson RO,Solovyov A,Greenbaum BD,Sanders C,Vi

    更新日期:2017-05-04 00:00:00

  • Isolation of the bovine and human genes for Müllerian inhibiting substance and expression of the human gene in animal cells.

    abstract::We have isolated the bovine and human genes for Müllerian inhibiting substance (MIS), a testicular glycoprotein that causes regression of the Müllerian duct during development of the male embryo. The mRNA sequence of bovine MIS, determined from an analysis of cDNA and genomic clones, codes for a protein of 575 amino a...


    pub_type: 杂志文章


    authors: Cate RL,Mattaliano RJ,Hession C,Tizard R,Farber NM,Cheung A,Ninfa EG,Frey AZ,Gash DJ,Chow EP

    更新日期:1986-06-06 00:00:00

  • The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth.

    abstract::Exchange factors for ARF GTPases (ARF-GEFs) regulate vesicle trafficking in a variety of organisms. The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of the plant hormone auxin. Mutations in GNOM lead to development...


    pub_type: 杂志文章


    authors: Geldner N,Anders N,Wolters H,Keicher J,Kornberger W,Muller P,Delbarre A,Ueda T,Nakano A,Jürgens G

    更新日期:2003-01-24 00:00:00

  • Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis.

    abstract::Yeast mutants lacking meiotic proteins Zip1, Zip2, Zip3, Mer3, and/or Msh5 (ZMMs) were analyzed for recombination, synaptonemal complex (SC), and meiotic progression. At 33 degrees C, recombination-initiating double-strand breaks (DSBs) and noncrossover products (NCRs) form normally while formation of single-end invas...


    pub_type: 杂志文章


    authors: Börner GV,Kleckner N,Hunter N

    更新日期:2004-04-02 00:00:00

  • Primary antibody-forming cells and secondary B cells are generated from separate precursor cell subpopulations.

    abstract::Two precursor cell subpopulations have been isolated from the spleen cells of nonimmune mice. The major B cell subpopulation binds high levels of the J11D monoclonal antibody and, upon T cell-dependent antigenic stimulation, gives rise to primary antibody-forming cell clones but not secondary B cells. A minority of th...


    pub_type: 杂志文章


    authors: Linton PJ L,Decker DJ,Klinman NR

    更新日期:1989-12-22 00:00:00

  • A Blueprint for Characterizing Senescence.

    abstract::Given the heterogeneity of senescent cells, our knowledge of both the drivers and consequences of cellular senescence in tissues and organs remains limited, as is our understanding of how this process could be harnessed for human health. Here we identified five broad areas that would help propel the field forward. ...


    pub_type: 杂志文章


    authors: Roy AL,Sierra F,Howcroft K,Singer DS,Sharpless N,Hodes RJ,Wilder EL,Anderson JM

    更新日期:2020-11-25 00:00:00

  • A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana.

    abstract::Infectious disease is both a major force of selection in nature and a prime cause of yield loss in agriculture. In plants, disease resistance is often conferred by nucleotide-binding leucine-rich repeat (NLR) proteins, intracellular immune receptors that recognize pathogen proteins and their effects on the host. Consi...


    pub_type: 杂志文章


    authors: Van de Weyer AL,Monteiro F,Furzer OJ,Nishimura MT,Cevik V,Witek K,Jones JDG,Dangl JL,Weigel D,Bemm F

    更新日期:2019-08-22 00:00:00

  • Pdd1p, a novel chromodomain-containing protein, links heterochromatin assembly and DNA elimination in Tetrahymena.

    abstract::During Tetrahymena conjugation, programmed DNA degradation occurs in two separate nuclei. Thousands of germline-specific deletion elements are removed from the genome of the developing somatic macronucleus, and the old parental macronucleus is degraded by an apoptotic mechanism. An abundant polypeptide, Pdd1p (formerl...


    pub_type: 杂志文章


    authors: Madireddi MT,Coyne RS,Smothers JF,Mickey KM,Yao MC,Allis CD

    更新日期:1996-10-04 00:00:00

  • Crystal structures of T. brucei MRP1/MRP2 guide-RNA binding complex reveal RNA matchmaking mechanism.

    abstract::The mitochondrial RNA binding proteins MRP1 and MRP2 form a heteromeric complex that functions in kinetoplastid RNA editing. In this process, MRP1/MRP2 serves as a matchmaker by binding to guide RNAs and facilitating their hybridization with cognate preedited mRNAs. To understand the mechanism by which this complex pe...


    pub_type: 杂志文章


    authors: Schumacher MA,Karamooz E,Zíková A,Trantírek L,Lukes J

    更新日期:2006-08-25 00:00:00

  • Amyloid-like aggregates sequester numerous metastable proteins with essential cellular functions.

    abstract::Protein aggregation is linked with neurodegeneration and numerous other diseases by mechanisms that are not well understood. Here, we have analyzed the gain-of-function toxicity of artificial β sheet proteins that were designed to form amyloid-like fibrils. Using quantitative proteomics, we found that the toxicity of ...


    pub_type: 杂志文章


    authors: Olzscha H,Schermann SM,Woerner AC,Pinkert S,Hecht MH,Tartaglia GG,Vendruscolo M,Hayer-Hartl M,Hartl FU,Vabulas RM

    更新日期:2011-01-07 00:00:00

  • The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro.

    abstract::Retroviral DNA integration involves a coordinated set of DNA cutting and joining reactions. We find that the IN protein of Moloney murine leukemia virus (MoMLV) is the only viral protein required to accomplish these reactions in vitro. IN protein has a site-specific nuclease activity that cleaves 2 nucleotides from th...


    pub_type: 杂志文章


    authors: Craigie R,Fujiwara T,Bushman F

    更新日期:1990-08-24 00:00:00

  • Molecular architecture of annelid nerve cord supports common origin of nervous system centralization in bilateria.

    abstract::To elucidate the evolutionary origin of nervous system centralization, we investigated the molecular architecture of the trunk nervous system in the annelid Platynereis dumerilii. Annelids belong to Bilateria, an evolutionary lineage of bilateral animals that also includes vertebrates and insects. Comparing nervous sy...


    pub_type: 杂志文章


    authors: Denes AS,Jékely G,Steinmetz PR,Raible F,Snyman H,Prud'homme B,Ferrier DE,Balavoine G,Arendt D

    更新日期:2007-04-20 00:00:00

  • Wild-type p53 restores cell cycle control and inhibits gene amplification in cells with mutant p53 alleles.

    abstract::Loss of cell cycle control and acquisition of chromosomal rearrangements such as gene amplification often occur during tumor progression, suggesting that they may be correlated. We show here that the wild-type p53 allele is lost when fibroblasts from patients with the Li-Fraumeni syndrome (LFS) are passaged in vitro. ...


    pub_type: 杂志文章


    authors: Yin Y,Tainsky MA,Bischoff FZ,Strong LC,Wahl GM

    更新日期:1992-09-18 00:00:00

  • The Drosophila caspase inhibitor DIAP1 is essential for cell survival and is negatively regulated by HID.

    abstract::Drosophila Reaper (RPR), Head Involution Defective (HID), and GRIM induce caspase-dependent cell death and physically interact with the cell death inhibitor DIAP1. Here we show that HID blocks DIAP1's ability to inhibit caspase activity and provide evidence suggesting that RPR and GRIM can act similarly. Based on thes...


    pub_type: 杂志文章


    authors: Wang SL,Hawkins CJ,Yoo SJ,Müller HA,Hay BA

    更新日期:1999-08-20 00:00:00

  • Rickettsia Sca4 Reduces Vinculin-Mediated Intercellular Tension to Promote Spread.

    abstract::Spotted fever group (SFG) rickettsiae are human pathogens that infect cells in the vasculature. They disseminate through host tissues by a process of cell-to-cell spread that involves protrusion formation, engulfment, and vacuolar escape. Other bacterial pathogens rely on actin-based motility to provide a physical for...


    pub_type: 杂志文章


    authors: Lamason RL,Bastounis E,Kafai NM,Serrano R,Del Álamo JC,Theriot JA,Welch MD

    更新日期:2016-10-20 00:00:00

  • Cellular uptake of the tat protein from human immunodeficiency virus.

    abstract::While developing an assay to measure the activity of the tat protein from human immunodeficiency virus 1 (HIV-1), we discovered that the purified protein could be taken up by cells growing in tissue culture and subsequently trans-activate the viral promoter. Trans-activation is dramatically increased by a variety of l...


    pub_type: 杂志文章


    authors: Frankel AD,Pabo CO

    更新日期:1988-12-23 00:00:00