Abstract：
:Radioactive alpha factor is degraded to discrete biologically inactive fragments by the target a cells of S. cerevisiae, but not by alpha cells which make the pheromone. The pattern of cleavage products and sequence analysis of one fragment indicated that the first scission occurred between leucine 6 and lysine 7. The protease inhibitors tosyl-L-argininyl-methyl ester (TAME), tosyl-L-lysyl-chloromethylketone (TLCK) and N-acetyl-L-leucyl-L-leucyl-L-argininal (leupeptin) markedly prolonged the period of G1 arrest in a cells exposed to alpha factor, while other standard protease inhibitors had little or no effect. The presence of TAME and leupeptin, or TLCK, reduced the rate of degradation of radioactively labeled alpha factor by a cells. Intact yeast cells have apparent esterase and amidase activities that are blocked by the same spectrum of inhibitors that potentiate alpha factor action. Purified alpha factor is a competitive inhibitor of these hydrolytic activities. The activities are present in yeast mutants which have greatly reduced levels of the three major vacuole-associated proteases (A, B and C) or which carry an ochre mutation in the major neutral protease (B). These observations indicate that the inactivation of alpha factor is due to endoproteolytic cleavage, the destruction of the pheromone is required to overcome its effects on growth and that degradation of the molecule may involve surface bound endopeptidase(s).
journal_name
Celljournal_title
Cellauthors
Ciejek E,Thorner Jdoi
10.1016/0092-8674(79)90117-xsubject
Has Abstractpub_date
1979-11-01 00:00:00pages
623-35issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(79)90117-Xjournal_volume
18pub_type
杂志文章相关文献
CELL文献大全abstract：:The elucidation of the genetic code remains among the most influential discoveries in biology. While innumerable studies have validated the general universality of the code and its value in predicting and analyzing protein coding sequences, established and emerging work has also suggested that full genome decryption m...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2016.09.022
更新日期：2016-12-15 00:00:00
abstract：:We performed a comprehensive assessment of rare inherited variation in autism spectrum disorder (ASD) by analyzing whole-genome sequences of 2,308 individuals from families with multiple affected children. We implicate 69 genes in ASD risk, including 24 passing genome-wide Bonferroni correction and 16 new ASD risk gen...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.07.015
更新日期：2019-08-08 00:00:00
abstract：:Protein kinase C (PKC) isozymes have remained elusive cancer targets despite the unambiguous tumor promoting function of their potent ligands, phorbol esters, and the prevalence of their mutations. We analyzed 8% of PKC mutations identified in human cancers and found that, surprisingly, most were loss of function and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.01.001
更新日期：2015-01-29 00:00:00
abstract：:Expression of human immunodeficiency virus type 1 structural proteins requires both the viral Rev trans-activator and its cis-acting RNA target sequence, the Rev response element (RRE). The RRE has been mapped to a conserved region of the HIV-1 env gene and is predicted to form a complex, highly stable RNA stem-loop s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90670-a
更新日期：1990-02-23 00:00:00
abstract：:GAL4 derivatives containing an activation domain alleviated repression of a promoter during nucleosome assembly. A GAL4 derivative lacking an activation domain stably bound the promoter during nucleosome assembly but was not sufficient to preserve promoter function. The activation domain of GAL4 derivatives was essent...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90237-s
更新日期：1991-02-08 00:00:00
abstract：:Protease-activated receptors (PARs) are a unique class of G protein-coupled receptors that play critical roles in thrombosis, inflammation, and vascular biology. PAR1 is proposed to be involved in the invasive and metastatic processes of various cancers. However, the protease responsible for activating the proinvasive...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.12.018
更新日期：2005-02-11 00:00:00
abstract：:The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5' end map positions to those reported for in v...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90343-x
更新日期：1984-03-01 00:00:00
abstract：:The 75 kDa tumor necrosis factor receptor (TNFR2) transduces extracellular signals via receptor-associated cytoplasmic proteins. Two of these signal transducers, TRAF1 and TRAF2, were isolated and characterized previously. We report here the biochemical purification and subsequent molecular cloning of two novel TNFR2-...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(95)90149-3
更新日期：1995-12-29 00:00:00
abstract：:Fluorescent proteins are commonly used to label cells across organisms, but the unmodified forms cannot control biological activities. Using GFP-binding proteins derived from Camelid antibodies, we co-opted GFP as a scaffold for inducing formation of biologically active complexes, developing a library of hybrid transc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.07.021
更新日期：2013-08-15 00:00:00
abstract：:The C-terminal domain (CTD) of the largest subunit of yeast RNA polymerase II contains 26-27 tandem copies of a conserved heptapeptide of unknown function. Yeast strains whose CTD contains ten heptamers are viable but defective for transcription of the INO1 gene and cold sensitive for growth. Deletion of the SIN1 gene...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90268-4
更新日期：1991-03-22 00:00:00
abstract：:Isolated human mitochondria containing a mitochondrial DNA (mtDNA) coded chloramphenicol resistance marker were injected into cells from two different human sensitive cell lines, 143BTK- and HT1080-6TG, which had been partially depleted of their mtDNA by ethidium bromide treatment. On the basis of the available eviden...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90423-0
更新日期：1988-03-25 00:00:00
abstract：:We show that a functional component of the C. elegans mitotic machinery regulates X chromosome gene expression. This protein, MIX-1, is a member of the dosage compensation complex that associates specifically with hermaphrodite X chromosomes to reduce their gene expression during interphase. MIX-1 also associates with...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80920-4
更新日期：1998-01-23 00:00:00
abstract：:The Mixed-Lineage Leukemia (MLL) protein is a histone methyltransferase that is mutated in clinically and biologically distinctive subsets of acute leukemia. MLL normally associates with a cohort of highly conserved cofactors to form a macromolecular complex that includes menin, a product of the MEN1 tumor suppressor ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.09.025
更新日期：2005-10-21 00:00:00
abstract：:This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90026-6
更新日期：1979-03-01 00:00:00
abstract：:The Drosophila transforming growth factor beta (TGF-beta) homolog Decapentaplegic (Dpp) acts as a morphogen that forms a long-range concentration gradient to direct the anteroposterior patterning of the wing. Both planar transcytosis initiated by Dynamin-mediated endocytosis and extracellular diffusion have been propo...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2004.09.031
更新日期：2004-10-15 00:00:00
abstract：:The pandemic has impacted every scientist differently. Many negative impacts are frequently discussed. Here we highlight unexpected positives that we have found and hope will persist: improved access to experts; deeper and broader human engagement among colleagues, collaborators, and competitors; and significant democ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.09.027
更新日期：2020-10-01 00:00:00
abstract：:We have purified and characterized a modified peptide pheromone that accumulates in culture medium as B. subtilis grows to high density. This pheromone is required for the development of genetic competence. When added to cells at low density, the pheromone induces the premature development of competence. The peptide m...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90313-1
更新日期：1994-04-22 00:00:00
abstract：:We have prepared three monoclonal antibodies against human epidermal keratins. These antibodies were highly specific for keratins and, in combination, recognized all major epidermal keratins of several mammalian species. We have used these antibodies to study the tissue distribution of epidermis-related keratins. In v...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90234-3
更新日期：1982-09-01 00:00:00
abstract：:DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins. dmc1 mutants are defective in reciprocal recombination, accumulate double-strand break (DSB) recombination intermediates, fail to form normal synaptonemal complex (SC), and arrest late in meiotic prophase. dmc1 ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90446-j
更新日期：1992-05-01 00:00:00
abstract：:We describe a new repressible binary expression system based on the regulatory genes from the Neurospora qa gene cluster. This "Q system" offers attractive features for transgene expression in Drosophila and mammalian cells: low basal expression in the absence of the transcriptional activator QF, high QF-induced expre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.02.025
更新日期：2010-04-30 00:00:00
abstract：:The histone tails on the nucleosome surface are subject to enzyme-catalyzed modifications that may, singly or in combination, form a code specifying patterns of gene expression. Recent papers provide insights into how a combinatorial code might be set and read. They show how modification of one residue can influence t...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s0092-8674(02)01080-2
更新日期：2002-11-01 00:00:00
abstract：:We determined the nucleotide sequences of all coding regions and a significant part of the flanking regions of the chicken c-src gene, which is a cellular homolog of the v-src gene of Rous sarcoma virus. The c-src gene consists of 12 exons; the boundaries of the exons were determined by assuming that the amino acid se...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90073-9
更新日期：1983-03-01 00:00:00
abstract：:As in eukaryotes, bacterial genomes are not randomly folded. Bacterial genetic information is generally carried on a circular chromosome with a single origin of replication from which two replication forks proceed bidirectionally toward the opposite terminus region. Here, we investigate the higher-order architecture o...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.12.027
更新日期：2018-02-08 00:00:00
abstract：:Characteristic of chronic myelogenous leukemia (CML) is the presence of the chimeric p210(bcr-abl) protein possessing elevated protein tyrosine kinase activity relative to normal c-abl tyrosine kinase. Hematopoietic progenitors isolated from CML patients in the chronic phase contain a constitutively tyrosine-phosphory...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81840-1
更新日期：1997-01-24 00:00:00
abstract：:Models of transcription are often built around a picture of RNA polymerase and transcription factors (TFs) acting on a single copy of a promoter. However, most TFs are shared between multiple genes with varying binding affinities. Beyond that, genes often exist at high copy number-in multiple identical copies on the c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.02.022
更新日期：2014-03-13 00:00:00
abstract：:Despite the obvious importance of epithelial stem cells in tissue homeostasis and tumorigenesis, little is known about their specific location or biological characteristics. Using 3H-thymidine labeling, we have identified a subpopulation of corneal epithelial basal cells, located in the peripheral cornea in a region c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(89)90958-6
更新日期：1989-04-21 00:00:00
abstract：:We analyzed a family of proteins from hepatoma cell nuclei that bind to interleukin-6 responsive elements (IL-6REs) of several acute-phase genes. This family is characterized by leucine zipper domains compatible with that of the CCAAT/enhancer binding protein (C/EBP). A cDNA clone coding for a member of the family, IL...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90459-r
更新日期：1990-11-02 00:00:00
abstract：:Synergistic bispecific antibodies against HIV exhibit extraordinary potency and breadth of neutralization as promising candidates for treatment and prevention. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2016.06.004
更新日期：2016-06-16 00:00:00
abstract：:In this issue of Cell, Noma et al. (2006) show that B-boxes and TFIIIC limit the spread of heterochromatin at the silent mat region in the fission yeast genome. Global analysis of TFIIIC distribution revealed dispersed sites of association that coalesce at the nuclear periphery, suggesting that TFIIIC may act as a bar...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2006.05.016
更新日期：2006-06-02 00:00:00
abstract：:RNA transcripts are subject to posttranscriptional gene regulation involving hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) expressed in a cell-type dependent fashion. We developed a cell-based crosslinking approach to determine at high resolution and transcriptome...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.03.009
更新日期：2010-04-02 00:00:00
