Abstract：
:Human autoantibodies that recognize the spindle poles of mammals, plants, and insects were found to recognize two antigens in yeast. One of these proteins, called SPA1 (for Spindle Pole Antigen), is antigenically related to the spindle poles of a diverse set of organisms. The gene encoding SPA1 was cloned by immunoscreening a lambda gt11 yeast genomic DNA expression library with autoantibody probes. Mutational analysis of the SPA1 gene demonstrates that it is important for cell growth, chromosome segregation, and other cellular processes; spa1 mutants are viable but grow poorly at 30 degrees C, missegregate chromosomes at an increased frequency, and often contain deformed spindles. A significant fraction of spa1 mutant cells contain two or more nuclei, and others contain none; these abnormal cells may arise through a nuclear migration defect. Thus SPA1 represents a new fidelity gene that is important for chromosome segregation and other mitotic functions.
journal_name
Celljournal_title
Cellauthors
Snyder M,Davis RWdoi
10.1016/s0092-8674(88)90977-4subject
Has Abstractpub_date
1988-09-09 00:00:00pages
743-54issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(88)90977-4journal_volume
54pub_type
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