Abstract：
:It has been proposed that the initiator protein RepA is rate limiting for mini-P1 plasmid replication, and that the role of the plasmid copy number control locus is to sequester the initiator and thus reduce replication. This proposal appears inconsistent with the observation that RepA is autoregulated, since the protein lost by sequestration should be replenished. A resolution of this autoregulation-sequestration paradox is possible if the sequestered RepA, unavailable for replication, is still available for promoter repression. We demonstrate that RepA binds to the control locus and to the promoter region simultaneously, causing the intervening DNA to loop. DNA looping could provide the requisite mechanism by which RepA bound to the control locus might exert repression.
journal_name
Celljournal_title
Cellauthors
Chattoraj DK,Mason RJ,Wickner SHdoi
10.1016/0092-8674(88)90468-0subject
Has Abstractpub_date
1988-02-26 00:00:00pages
551-7issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(88)90468-0journal_volume
52pub_type
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