Abstract:
:The Drosha-DGCR8 complex, also known as Microprocessor, is essential for microRNA (miRNA) maturation. Drosha functions as the catalytic subunit, while DGCR8 (also known as Pasha) recognizes the RNA substrate. Although the action mechanism of this complex has been intensively studied, it remains unclear how Drosha and DGCR8 are regulated and if these proteins have any additional role(s) apart from miRNA processing. Here, we report that Drosha and DGCR8 regulate each other posttranscriptionally. The Drosha-DGCR8 complex cleaves the hairpin structures embedded in the DGCR8 mRNA and thereby destabilizes the mRNA. We further find that DGCR8 stabilizes the Drosha protein via protein-protein interaction. This crossregulation between Drosha and DGCR8 may contribute to the homeostatic control of miRNA biogenesis. Furthermore, microarray analyses suggest that a number of mRNAs may be downregulated in a Microprocessor-dependent, miRNA-independent manner. Our study reveals a previously unsuspected function of Microprocessor in mRNA stability control.
journal_name
Celljournal_title
Cellauthors
Han J,Pedersen JS,Kwon SC,Belair CD,Kim YK,Yeom KH,Yang WY,Haussler D,Blelloch R,Kim VNdoi
10.1016/j.cell.2008.10.053subject
Has Abstractpub_date
2009-01-09 00:00:00pages
75-84issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(08)01490-6journal_volume
136pub_type
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