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The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway.

Abstract：

:We have investigated the in vivo functional role of rab5, a small GTPase associated with the plasma membrane and early endosomes. Wild-type rab5 or rab5-ile133, a mutant protein defective in GTP binding, was overexpressed in baby hamster kidney cells. In cells expressing the rab5ile 133 protein, the rate of endocytosis was decreased by 50% compared with normal, while the rate of recycling was not significantly affected. The morphology of early endosomes was also drastically changed by the mutant protein, which induced accumulation of small tubules and vesicles at the periphery of the cell. Surprisingly, overexpression of wild-type rab5 accelerated the uptake of endocytic markers and led to the appearance of atypically large early endosomes. We conclude that rab5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.

journal_name

Cell

journal_title

Cell

authors

Bucci C,Parton RG,Mather IH,Stunnenberg H,Simons K,Hoflack B,Zerial M

doi

10.1016/0092-8674(92)90306-w

subject

Has Abstract

pub_date

1992-09-04 00:00:00

pages

715-28

issue

5

eissn

0092-8674

issn

1097-4172

pii

0092-8674(92)90306-W

journal_volume

70

pub_type

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