Abstract：
:We have examined the chromatin structure of a repeated cluster of tRNA genes of Xenopus laevis, with particular attention to possible phase relationships between nucleosomes and the underlying tDNA sequence. In transcriptionally active liver and cultured kidney cells, essentially all tDNA is packaged into regularly spaced nucleosomes, but no detectable phase relationship exists between nucleosomes and DNA sequence. Only in transcriptionally inactive erythrocytes do nucleosomes on all tDNA repeats occur in one predominant phase. In this phasing arrangement, nucleosomes are regularly spaced with the exception of a 250 base pair gap occurring once per 3.18 kilobase repeat unit. This arrangement suggests that nucleosome phasing may aid in the organization of a specific higher order packaging, with each unit of packaging containing one 3.18 kilobase repeat.
journal_name
Celljournal_title
Cellauthors
Bryan PN,Hofstetter H,Birnstiel MLdoi
10.1016/0092-8674(81)90387-1subject
Has Abstractpub_date
1981-12-01 00:00:00pages
459-66issue
3 Pt 2eissn
0092-8674issn
1097-4172pii
0092-8674(81)90387-1journal_volume
27pub_type
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