Abstract：
:Nontransformed pre-B cells were induced to differentiate in vitro along several different but predictable pathways with only dendritic cells (DC) and concanavalin A-stimulated T lymphocytes. DC-T from spleen induced secretion of only IgM, whereas DC-T from Peyer's patches induced high levels of IgA and intermediate levels of IgM and IgG. Both the isotype of antibody secreted and the extent of pre-B cell differentiation were determined by the lymphoid tissue source of DC, not of T cells. The pre-B cells synthesizing detectable levels of only the IgM heavy chain had cytoplasmic RNA transcripts from both light chain constant region genes and from the entire length of the heavy chain constant locus. In cells secreting IgM there were deletions in the DNA flanking the Cmu coding region.
journal_name
Celljournal_title
Cellauthors
Spalding DM,Griffin JAdoi
10.1016/0092-8674(86)90472-1subject
Has Abstractpub_date
1986-02-14 00:00:00pages
507-15issue
3eissn
0092-8674issn
1097-4172pii
0092-8674(86)90472-1journal_volume
44pub_type
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