Abstract:
:The single, chloroplast encoded gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) from Euglena gracilis is found to contain nine intervening sequences. The intervening sequences were identified by heteroduplex analysis between Euglena rbcL and the non-intron-containing rbcL from Spinacea oleracea, by electron microscopy of Euglena rbcL DNA-mRNA hybrids, and by cloning, restriction endonuclease analysis, and partial DNA sequencing. The identification, locus, and coding properties for six of ten exons was confirmed by partial DNA sequence analysis. Each of the nine introns in the approximately 6.5 kb rbcL locus is approximately 0.5 kb in length. The DNA sequence of five 3'-intron/5'-exon and four 3'-exon/5'-intron boundaries are highly conserved. A proposed consensus sequence is (formula; see text) These conserved sequences could play a role in an mRNA splicing mechanism in chloroplasts analogous to that in eucaryotic nuclei.
journal_name
Celljournal_title
Cellauthors
Koller B,Gingrich JC,Stiegler GL,Farley MA,Delius H,Hallick RBdoi
10.1016/0092-8674(84)90247-2subject
Has Abstractpub_date
1984-02-01 00:00:00pages
545-53issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(84)90247-2journal_volume
36pub_type
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