Francis Crick: A Singular Approach to Scientific Discovery.


:Francis' office window (at the Salk) commanded a panorama of the Pacific. "This grand natural scene was a physical correlate of Francis's intellectual world: wide-ranging, brilliantly lit, a little overawing, but also immensely inviting and above all an exciting place to be." (Mitchison, 2004).






Lawrence PA




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2016-12-01 00:00:00
















  • Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA.

    abstract::Methylation of deoxycytidine incorporated by DNA excision-repair was studied in human diploid fibroblasts following damage with ultraviolet radiation, N-methyl-N-nitrosourea, or N-acetoxy-2-acetylaminofluorene. In confluent, nondividing cells, methylation in repair patches induced by all three agents is slow and incom...


    pub_type: 杂志文章


    authors: Kastan MB,Gowans BJ,Lieberman MW

    更新日期:1982-09-01 00:00:00

  • Sequence organization of a cloned tDNA met fragment from Xenopus laevis.

    abstract::3.18 kb fragments of X. laevis DNA coding for tRNA 1 met have been inserted into a lambda vector via Hind III termini and cloned in E. coli. The organization of one cloned fragment has been analyzed by restriction endonuclease digestion and RNA-DNA hybridization. From the distribution of sites for three enzymes, this ...


    pub_type: 杂志文章


    authors: Clarkson SG,Kurer V,Smith HO

    更新日期:1978-07-01 00:00:00

  • Structural basis of caspase-7 inhibition by XIAP.

    abstract::The inhibitor of apoptosis (IAP) proteins suppress cell death by inhibiting the catalytic activity of caspases. Here we present the crystal structure of caspase-7 in complex with a potent inhibitory fragment from XIAP at 2.45 A resolution. An 18-residue XIAP peptide binds the catalytic groove of caspase-7, making exte...


    pub_type: 杂志文章


    authors: Chai J,Shiozaki E,Srinivasula SM,Wu Q,Datta P,Alnemri ES,Shi Y

    更新日期:2001-03-09 00:00:00

  • How TriC folds tricky proteins.

    abstract::How chaperonins orchestrate the successful folding of even the most elaborate of proteins is a question of central importance. In two recent studies in Cell by Joachimiak et al. and Freund et al., a new class of TRiC substrate is identified, and how the chaperonin exploits its different subunits to extend its substrat...


    pub_type: 评论,杂志文章


    authors: Zhuravleva A,Radford SE

    更新日期:2014-12-04 00:00:00

  • Structure and mechanism of a metal-sensing regulatory RNA.

    abstract::Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan ribos...


    pub_type: 杂志文章


    authors: Dann CE 3rd,Wakeman CA,Sieling CL,Baker SC,Irnov I,Winkler WC

    更新日期:2007-09-07 00:00:00

  • Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans.

    abstract::The sulfated glycosaminoglycan, heparin, was found to release 125I-labeled low density lipoprotein (125I-LDL) from its receptor site on the surface of normal human fibroblasts. Measurement of the amount of 125I-LDL released by heparin permitted the resolution of the total cellular uptake of 125I-LDL at 37 degrees C in...


    pub_type: 杂志文章


    authors: Goldstein JL,Basu SK,Brunschede GY,Brown MS

    更新日期:1976-01-01 00:00:00

  • The secret life of Smad4.

    abstract::Smad4 is an important mediator of signaling by TGFbeta family members that is presumed to be controlled by the phosphorylation status of its partners, the receptor-regulated R-Smads. In this issue, Dupont et al. (2009) now reveal that Smad4 itself is subject to cycles of ubiquitination and deubiquitination that regula...


    pub_type: 评论,杂志文章


    authors: Wrana JL

    更新日期:2009-01-09 00:00:00

  • SnapShot: Electrochemical Communication in Biofilms.

    abstract::The role of electricity in biological systems was first appreciated through electrical stimulation experiments performed by Luigi Galvani in the 18th century. These pioneering experiments demonstrated that the behavior of living tissues is governed by the flow of electrochemical species-an insight that gave rise to th...


    pub_type: 杂志文章


    authors: Lee DD,Prindle A,Liu J,Süel GM

    更新日期:2017-06-29 00:00:00

  • Structure of DNA within three isometric bacteriophages.

    abstract::This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage...


    pub_type: 杂志文章


    authors: Kosturko LD,Hogan M,Dattagupta N

    更新日期:1979-03-01 00:00:00

  • Ras1 promotes cellular growth in the Drosophila wing.

    abstract::The Ras GTPase links extracellular mitogens to intracellular mechanisms that control cell proliferation. To understand how Ras regulates proliferation in vivo, we activated or inactivated Ras in cell clones in the developing Drosophila wing. Cells lacking Ras were smaller, had reduced growth rates, accumulated in G1, ...


    pub_type: 杂志文章


    authors: Prober DA,Edgar BA

    更新日期:2000-02-18 00:00:00

  • Role of the Xist gene in X chromosome choosing.

    abstract::In female mammals a "random choice" mechanism decides which of the two X chromosomes will be inactivated. It has been postulated that Xist is crucial for heterochromatinization and thus functions downstream of the choice mechanism. Here we report that females heterozygous for an internal deletion in the Xist gene, whi...


    pub_type: 杂志文章


    authors: Marahrens Y,Loring J,Jaenisch R

    更新日期:1998-03-06 00:00:00

  • Polarized sorting of viral glycoproteins to the axon and dendrites of hippocampal neurons in culture.

    abstract::Cultured hippocampal neurons were infected with a temperature-sensitive mutant of vesicular stomatitis virus (VSV) and a wild-type strain of the avian influenza fowl plague virus (FPV). The intracellular distribution of viral glycoproteins was monitored by immunofluorescence microscopy. In mature, fully polarized neur...


    pub_type: 杂志文章


    authors: Dotti CG,Simons K

    更新日期:1990-07-13 00:00:00

  • Comparative analysis of the structural organization of two closely related vitellogenin genes in X. laevis.

    abstract::The structural organization of the two closely related vitellogenin genes A1 and A2 has been determined and compared by electron microscopy. In both genes the mRNA-coding sequence of 6 kb is interrupted 33 times, leading to a total gene length of 21 kb for gene A1 and 16 kb for gene A2. Thus both genes have a mean exo...


    pub_type: 杂志文章


    authors: Wahli W,Dawid IB,Wyler T,Weber R,Ryffel GU

    更新日期:1980-05-01 00:00:00

  • Balancing acts: molecular control of mammalian iron metabolism.

    abstract::Iron is ubiquitous in the environment and in biology. The study of iron biology focuses on physiology and homeostasis-understanding how cells and organisms regulate their iron content, how diverse tissues orchestrate iron allocation, and how dysregulated iron homeostasis leads to common hematological, metabolic, and n...


    pub_type: 杂志文章,评审


    authors: Hentze MW,Muckenthaler MU,Andrews NC

    更新日期:2004-04-30 00:00:00

  • Optimal Decoding of Cellular Identities in a Genetic Network.

    abstract::In developing organisms, spatially prescribed cell identities are thought to be determined by the expression levels of multiple genes. Quantitative tests of this idea, however, require a theoretical framework capable of exposing the rules and precision of cell specification over developmental time. We use the gap gene...


    pub_type: 杂志文章


    authors: Petkova MD,Tkačik G,Bialek W,Wieschaus EF,Gregor T

    更新日期:2019-02-07 00:00:00

  • Protein phosphorylation at tyrosine is induced by the v-erbB gene product in vivo and in vitro.

    abstract::The v-erbB gene product of avian erythroblastosis virus (AEV) has extensive homology with the receptor for epidermal growth factor (EGF). We report here that chicken embryo fibroblasts (CEF) transformed by AEV show enhanced tyrosine phosphorylation of a number of cellular polypeptides, including the 36 kd protein, whi...


    pub_type: 杂志文章


    authors: Gilmore T,DeClue JE,Martin GS

    更新日期:1985-03-01 00:00:00

  • Genome sequencing reveals loci under artificial selection that underlie disease phenotypes in the laboratory rat.

    abstract::Large numbers of inbred laboratory rat strains have been developed for a range of complex disease phenotypes. To gain insights into the evolutionary pressures underlying selection for these phenotypes, we sequenced the genomes of 27 rat strains, including 11 models of hypertension, diabetes, and insulin resistance, al...


    pub_type: 杂志文章


    authors: Atanur SS,Diaz AG,Maratou K,Sarkis A,Rotival M,Game L,Tschannen MR,Kaisaki PJ,Otto GW,Ma MC,Keane TM,Hummel O,Saar K,Chen W,Guryev V,Gopalakrishnan K,Garrett MR,Joe B,Citterio L,Bianchi G,McBride M,Dominiczak A

    更新日期:2013-08-01 00:00:00

  • SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion.

    abstract::SecA, the peripheral subunit of E. coli preprotein translocase, alternates between a membrane inserted and a deinserted state as part of the catalytic cycle of preprotein translocation. When SecA is complexed with SecY/E and preprotein, ATP drives a profound conformational change, leading to membrane insertion of a 30...


    pub_type: 杂志文章


    authors: Economou A,Wickner W

    更新日期:1994-09-09 00:00:00

  • Antisense RNA inhibits expression of membrane skeleton protein 4.1 during embryonic development of Xenopus.

    abstract::Plasmids expressing partial-length sense or antisense protein 4.1 RNA were microinjected into fertilized Xenopus eggs. Nuclease protection assays reveal that antisense protein 4.1 RNA lead to the specific loss of endogenous protein 4.1 transcripts after midblastula transition, with no effect on the levels of three unr...


    pub_type: 杂志文章


    authors: Giebelhaus DH,Eib DW,Moon RT

    更新日期:1988-05-20 00:00:00

  • Structure-guided reprogramming of human cGAS dinucleotide linkage specificity.

    abstract::Cyclic dinucleotides (CDNs) play central roles in bacterial pathogenesis and innate immunity. The mammalian enzyme cGAS synthesizes a unique cyclic dinucleotide (cGAMP) containing a 2'-5' phosphodiester linkage essential for optimal immune stimulation, but the molecular basis for linkage specificity is unknown. Here, ...


    pub_type: 杂志文章


    authors: Kranzusch PJ,Lee ASY,Wilson SC,Solovykh MS,Vance RE,Berger JM,Doudna JA

    更新日期:2014-08-28 00:00:00

  • Genome-wide map of nucleosome acetylation and methylation in yeast.

    abstract::Eukaryotic genomes are packaged into nucleosomes whose position and chemical modification state can profoundly influence regulation of gene expression. We profiled nucleosome modifications across the yeast genome using chromatin immunoprecipitation coupled with DNA microarrays to produce high-resolution genome-wide ma...


    pub_type: 杂志文章


    authors: Pokholok DK,Harbison CT,Levine S,Cole M,Hannett NM,Lee TI,Bell GW,Walker K,Rolfe PA,Herbolsheimer E,Zeitlinger J,Lewitter F,Gifford DK,Young RA

    更新日期:2005-08-26 00:00:00

  • A Mild PUM1 Mutation Is Associated with Adult-Onset Ataxia, whereas Haploinsufficiency Causes Developmental Delay and Seizures.

    abstract::Certain mutations can cause proteins to accumulate in neurons, leading to neurodegeneration. We recently showed, however, that upregulation of a wild-type protein, Ataxin1, caused by haploinsufficiency of its repressor, the RNA-binding protein Pumilio1 (PUM1), also causes neurodegeneration in mice. We therefore search...


    pub_type: 杂志文章


    authors: Gennarino VA,Palmer EE,McDonell LM,Wang L,Adamski CJ,Koire A,See L,Chen CA,Schaaf CP,Rosenfeld JA,Panzer JA,Moog U,Hao S,Bye A,Kirk EP,Stankiewicz P,Breman AM,McBride A,Kandula T,Dubbs HA,Macintosh R,Cardamone M

    更新日期:2018-02-22 00:00:00

  • A CK2-dependent mechanism for degradation of the PML tumor suppressor.

    abstract::The PML tumor suppressor controls key pathways for growth suppression, induction of apoptosis, and cellular senescence. PML loss occurs frequently in human tumors through unknown posttranslational mechanisms. Casein kinase 2 (CK2) is oncogenic and frequently upregulated in human tumors. Here we show that CK2 regulates...


    pub_type: 杂志文章


    authors: Scaglioni PP,Yung TM,Cai LF,Erdjument-Bromage H,Kaufman AJ,Singh B,Teruya-Feldstein J,Tempst P,Pandolfi PP

    更新日期:2006-07-28 00:00:00

  • The SR protein SRp38 represses splicing in M phase cells.

    abstract::SR proteins constitute a family of pre-mRNA splicing factors that play important roles in both constitutive and regulated splicing. Here, we describe one member of the family, which we call SRp38, with unexpected properties. Unlike other SR proteins, SRp38 cannot activate splicing and is essentially inactive in splici...


    pub_type: 杂志文章


    authors: Shin C,Manley JL

    更新日期:2002-11-01 00:00:00

  • The spliceosome: design principles of a dynamic RNP machine.

    abstract::Ribonucleoproteins (RNPs) mediate key cellular functions such as gene expression and its regulation. Whereas most RNP enzymes are stable in composition and harbor preformed active sites, the spliceosome, which removes noncoding introns from precursor messenger RNAs (pre-mRNAs), follows fundamentally different strategi...


    pub_type: 杂志文章,评审


    authors: Wahl MC,Will CL,Lührmann R

    更新日期:2009-02-20 00:00:00

  • Glia Get Neurons in Shape.

    abstract::Glial cells are essential components of the nervous system. In this issue, Singhvi et al. uncover cellular and molecular mechanisms through which C. elegans glia shape sensory neuron terminals and thus control animal thermosensing behaviors. ...


    pub_type: 评论,杂志文章


    authors: Sun LO,Barres BA

    更新日期:2016-05-05 00:00:00

  • The path of messenger RNA through the ribosome.

    abstract::Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back...


    pub_type: 杂志文章


    authors: Yusupova GZ,Yusupov MM,Cate JH,Noller HF

    更新日期:2001-07-27 00:00:00

  • Chicken myeloid stem cells infected by retroviruses carrying the v-fps oncogene do not require exogenous growth factors to differentiate in vitro.

    abstract::To determine the function of c-fps in chicken macrophages and granulocytic cells we have infected chicken bone marrow cells with retroviruses containing the v-fps oncogene. Normal chicken macrophage progenitors, M-CFCs, give rise to macrophage colonies in semisolid cultures when macrophage colony stimulating factor (M...


    pub_type: 杂志文章


    authors: Carmier JF,Samarut J

    更新日期:1986-01-17 00:00:00

  • Maternal-fetal immune tolerance, block by block.

    abstract::How difficult is to go from egg to implanted embryo? The evolution of placentation in eutherian mammals created enormous challenges, in particular to the maternal immune system, as the fetus expresses paternal antigens that are capable of triggering immune rejection. Samstein et al. reveal a role for inducible regulat...


    pub_type: 评论,杂志文章


    authors: Gobert M,Lafaille JJ

    更新日期:2012-07-06 00:00:00

  • Differential postendocytotic compartmentation in Xenopus oocytes is mediated by a specifically bound ligand.

    abstract::Xenopus laevis oocytes were used as a model system to study the intracellular fate of proteins incorporated by endocytosis. We found that the intracellular stability and compartmentation of proteins incorporated by receptor-mediated endocytosis differed substantially from that of proteins incorporated by nonspecific e...


    pub_type: 杂志文章


    authors: Opresko L,Wiley HS,Wallace RA

    更新日期:1980-11-01 00:00:00