Abstract:
:Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.
journal_name
Celljournal_title
Cellauthors
Takamori S,Holt M,Stenius K,Lemke EA,Grønborg M,Riedel D,Urlaub H,Schenck S,Brügger B,Ringler P,Müller SA,Rammner B,Gräter F,Hub JS,De Groot BL,Mieskes G,Moriyama Y,Klingauf J,Grubmüller H,Heuser J,Wieland F,Jahdoi
10.1016/j.cell.2006.10.030subject
Has Abstractpub_date
2006-11-17 00:00:00pages
831-46issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(06)01400-0journal_volume
127pub_type
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