Abstract:
:E. coli RecA protein promotes homologous pairing and reciprocal strand exchange reactions between duplex DNA molecules in vitro. Reaction intermediates contain Holliday junctions that are driven along the DNA at a maximal rate approaching 1000 bases per minute. T4 endonuclease VII cleaves Holliday junctions in vitro, and its inclusion in RecA-mediated reactions leads to the rapid formation of heteroduplex products. Product analysis indicates patch and splice recombinant molecules similar to those expected from in vivo recombination events. The combined formation and resolution of Holliday junctions has led us to propose a model for resolution based on the structure of RecA-DNA helices. One feature of this model is that resolution, which gives rise to the two types of recombinant product, may occur without need for isomerization of the junction.
journal_name
Celljournal_title
Cellauthors
Müller B,Jones C,Kemper B,West SCdoi
10.1016/0092-8674(90)90747-3subject
Has Abstractpub_date
1990-01-26 00:00:00pages
329-36issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(90)90747-3journal_volume
60pub_type
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