Fc receptor isoforms exhibit distinct abilities for coated pit localization as a result of cytoplasmic domain heterogeneity.


:Mouse macrophages and lymphocytes express two distinct isoforms of a single class of Fc receptor for IgG. The macrophage isoform (FcRII-B2) is identical to the lymphocyte isoform (FcRII-B1) except for an inframe insertion in the cytoplasmic tail of FcRII-B1 that increases its length from 47 to 94 amino acids. To determine the functional significance of this cytoplasmic domain variation, presumably the result of alternative mRNA splicing, we expressed both isoforms in receptor-negative fibroblasts. While FcRII-B2 mediated the efficient ligand internalization and delivery to lysosomes, endocytosis via FcRII-B1--and via a tailminus mutant--was relatively inefficient. This difference reflected the inability of FcRII-B1 (and the tailminus mutant) to accumulate in clathrin-coated pits. Thus, the FcRII-B2 cytoplasmic tail contains a domain needed for accumulation in coated pits, and this domain is disrupted by the 47 amino acid insertion in FcRII-B1.






Miettinen HM,Rose JK,Mellman I




Has Abstract


1989-07-28 00:00:00
















  • A symmetrical six-base-pair target site sequence determines Tn10 insertion specificity.

    abstract::Transposon Tn10 inserts at many sites in the bacterial chromosome, but preferentially inserts at particular hotspots. We believe we have identified the target DNA signal responsible for this specificity. We have determined the DNA sequences of 11 Tn10 insertion sites and identified a particular 6 base pair (bp) symmet...


    pub_type: 杂志文章


    authors: Halling SM,Kleckner N

    更新日期:1982-01-01 00:00:00

  • RAP-1 factor is necessary for DNA loop formation in vitro at the silent mating type locus HML.

    abstract::DNA fragments containing the silencers that flank the mating type genes at HML alpha are shown to bind specifically to the nuclear scaffold of yeast. The scaffold proteins are solubilized with urea and then renatured to form a soluble extract which allows reconstitution of sequence-specific DNA loops. At the silent ma...


    pub_type: 杂志文章


    authors: Hofmann JF,Laroche T,Brand AH,Gasser SM

    更新日期:1989-06-02 00:00:00

  • The homeodomain transcription factor Irx5 establishes the mouse cardiac ventricular repolarization gradient.

    abstract::Rhythmic cardiac contractions depend on the organized propagation of depolarizing and repolarizing wavefronts. Repolarization is spatially heterogeneous and depends largely on gradients of potassium currents. Gradient disruption in heart disease may underlie susceptibility to fatal arrhythmias, but it is not known how...


    pub_type: 杂志文章


    authors: Costantini DL,Arruda EP,Agarwal P,Kim KH,Zhu Y,Zhu W,Lebel M,Cheng CW,Park CY,Pierce SA,Guerchicoff A,Pollevick GD,Chan TY,Kabir MG,Cheng SH,Husain M,Antzelevitch C,Srivastava D,Gross GJ,Hui CC,Backx PH,Bruneau

    更新日期:2005-10-21 00:00:00

  • Genomic rearrangements in a mouse cell line containing integrated SV40 DNA.

    abstract::In the SV40-transformed mouse embryo fibroblast cell line SVT2/S, genomic rearrangements involving the SV40 DNA and flanking host sequences were identified by Southern blot hybridization using viral DNA as probe. No rearrangements of SV40 DNA integrated into nonpermissive mouse cells have been previously described. Th...


    pub_type: 杂志文章


    authors: Sager R,Anisowicz A,Howell N

    更新日期:1981-01-01 00:00:00

  • Growth hormone and the adipose conversion of 3T3 cells.

    abstract::Cultured preadipose 3T3 cells are induced or enabled to undergo adipose conversion in the presence of an extract of pituitary gland. Adipogenic activity is found in standard growth hormone preparations derived from different species. Further purification of rat growth hormone by several methods does not remove its adi...


    pub_type: 杂志文章


    authors: Morikawa M,Nixon T,Green H

    更新日期:1982-07-01 00:00:00

  • CLP1 founder mutation links tRNA splicing and maturation to cerebellar development and neurodegeneration.

    abstract::Neurodegenerative diseases can occur so early as to affect neurodevelopment. From a cohort of more than 2,000 consanguineous families with childhood neurological disease, we identified a founder mutation in four independent pedigrees in cleavage and polyadenylation factor I subunit 1 (CLP1). CLP1 is a multifunctional ...


    pub_type: 杂志文章


    authors: Schaffer AE,Eggens VR,Caglayan AO,Reuter MS,Scott E,Coufal NG,Silhavy JL,Xue Y,Kayserili H,Yasuno K,Rosti RO,Abdellateef M,Caglar C,Kasher PR,Cazemier JL,Weterman MA,Cantagrel V,Cai N,Zweier C,Altunoglu U,Satkin N

    更新日期:2014-04-24 00:00:00

  • Neuronal control of metabolism through nutrient-dependent modulation of tracheal branching.

    abstract::During adaptive angiogenesis, a key process in the etiology and treatment of cancer and obesity, the vasculature changes to meet the metabolic needs of its target tissues. Although the cues governing vascular remodeling are not fully understood, target-derived signals are generally believed to underlie this process. H...


    pub_type: 杂志文章


    authors: Linneweber GA,Jacobson J,Busch KE,Hudry B,Christov CP,Dormann D,Yuan M,Otani T,Knust E,de Bono M,Miguel-Aliaga I

    更新日期:2014-01-16 00:00:00

  • Fetal anemia and apoptosis of red cell progenitors in Stat5a-/-5b-/- mice: a direct role for Stat5 in Bcl-X(L) induction.

    abstract::The erythropoietin receptor (EpoR) is essential for production of red blood cells; a principal function of EpoR is to rescue committed erythroid progenitors from apoptosis. Stat5 is rapidly activated following EpoR stimulation, but its function in erythropoiesis has been unclear since adult Stat5a-/-5b-/- mice have no...


    pub_type: 杂志文章


    authors: Socolovsky M,Fallon AE,Wang S,Brugnara C,Lodish HF

    更新日期:1999-07-23 00:00:00

  • DICER1 loss and Alu RNA induce age-related macular degeneration via the NLRP3 inflammasome and MyD88.

    abstract::Alu RNA accumulation due to DICER1 deficiency in the retinal pigmented epithelium (RPE) is implicated in geographic atrophy (GA), an advanced form of age-related macular degeneration that causes blindness in millions of individuals. The mechanism of Alu RNA-induced cytotoxicity is unknown. Here we show that DICER1 def...


    pub_type: 杂志文章


    authors: Tarallo V,Hirano Y,Gelfand BD,Dridi S,Kerur N,Kim Y,Cho WG,Kaneko H,Fowler BJ,Bogdanovich S,Albuquerque RJ,Hauswirth WW,Chiodo VA,Kugel JF,Goodrich JA,Ponicsan SL,Chaudhuri G,Murphy MP,Dunaief JL,Ambati BK,Ogura Y

    更新日期:2012-05-11 00:00:00

  • Effects of LETS glycoprotein on cell motility.

    abstract::Addition of LETS glycoprotein to normal or transformed cells produces increased migration of the cells, as determined by formation of phagokinetic tracks on gold particle-coated coverslips. These tracks arise by a combination of phagocytosis of the gold particles and cellular migration. Increased motility is also evid...


    pub_type: 杂志文章


    authors: Ali IU,Hynes RO

    更新日期:1978-06-01 00:00:00

  • Scrapie prion protein contains a phosphatidylinositol glycolipid.

    abstract::The scrapie (PrPSc) and cellular (PrPC) prion proteins are encoded by the same gene, and their different properties are thought to arise from posttranslational modifications. We have found a phosphatidylinositol glycolipid on both PrPC and PrP 27-30 (derived from PrPSc by limited proteolysis at the amino terminus). Et...


    pub_type: 杂志文章


    authors: Stahl N,Borchelt DR,Hsiao K,Prusiner SB

    更新日期:1987-10-23 00:00:00

  • A unique cap(m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primers that initiate viral RNA transcription.

    abstract::We propose a mechanism for the priming of influenza viral RNA transcription by capped RNAs in which specific 5'-terminal fragments are cleaved from the capped RNAs by a virion-associated endonuclease. These fragments would serve as the actual primers for the initiation of transcription by the initial incorporation by ...


    pub_type: 杂志文章


    authors: Plotch SJ,Bouloy M,Ulmanen I,Krug RM

    更新日期:1981-03-01 00:00:00

  • alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow.

    abstract::Of the several families of adhesion receptors involved in leukocyte-endothelial cell interactions, only the selectins have been shown to initiate leukocyte interaction under physiologic shear; indeed, beta 2 (CD18) intergrins responsible for neutrophil arrest are unable to engage without prior selectin-mediated rollin...


    pub_type: 杂志文章


    authors: Berlin C,Bargatze RF,Campbell JJ,von Andrian UH,Szabo MC,Hasslen SR,Nelson RD,Berg EL,Erlandsen SL,Butcher EC

    更新日期:1995-02-10 00:00:00

  • A functional interaction between the C-terminal domain of RNA polymerase II and the negative regulator SIN1.

    abstract::The C-terminal domain (CTD) of the largest subunit of yeast RNA polymerase II contains 26-27 tandem copies of a conserved heptapeptide of unknown function. Yeast strains whose CTD contains ten heptamers are viable but defective for transcription of the INO1 gene and cold sensitive for growth. Deletion of the SIN1 gene...


    pub_type: 杂志文章


    authors: Peterson CL,Kruger W,Herskowitz I

    更新日期:1991-03-22 00:00:00

  • Both layers of the COPII coat come into view.

    abstract::Anterograde transport in the early secretory pathway is mediated by COPII-coated vesicles. Stagg et al. (2008) have now visualized the double-layered COPII coat using electron cryomicroscopy, providing insight into how coats are assembled to accommodate cargo of different sizes. ...


    pub_type: 评论,杂志文章


    authors: Hughson FM

    更新日期:2008-08-08 00:00:00

  • The secret life of ACE2 as a receptor for the SARS virus.

    abstract::The membrane-associated carboxypeptidase angiotensin-converting enzyme 2 (ACE2) is an essential regulator of heart function. Now, Li at al. identify and characterize an unexpected second function of ACE2 as a partner of the SARS-CoV spike glycoprotein in mediating virus entry and cell fusion. ...


    pub_type: 杂志文章


    authors: Dimitrov DS

    更新日期:2003-12-12 00:00:00

  • ParB-type DNA Segregation Proteins Are CTP-Dependent Molecular Switches.

    abstract::During cell division, newly replicated DNA is actively segregated to the daughter cells. In most bacteria, this process involves the DNA-binding protein ParB, which condenses the centromeric regions of sister DNA molecules into kinetochore-like structures that recruit the DNA partition ATPase ParA and the prokaroytic ...


    pub_type: 杂志文章


    authors: Osorio-Valeriano M,Altegoer F,Steinchen W,Urban S,Liu Y,Bange G,Thanbichler M

    更新日期:2019-12-12 00:00:00

  • Progesterone-induced meiosis in Xenopus laevis oocytes: a role for cAMP at the "maturation-promoting factor" level.

    abstract::Cholera toxin inhibition of progesterone-induced meiosis of Xenopus laevis oocytes in vitro has been correlated with increased cAMP levels. Inhibition of germinal vesicle breakdown (Gvbd) and cAMP increase occurred after a lag period of 2 hr, when cholera toxin was injected, or 4--5 hr, when applied externally. The ab...


    pub_type: 杂志文章


    authors: Schorderet-Slatkine S,Schorderet M,Boquet P,Godeau F,Baulieu EE

    更新日期:1978-12-01 00:00:00

  • Identification of base mismatches recognized by the heteroduplex-DNA-repair system of Streptococcus pneumoniae.

    abstract::The susceptibility to repair of particular base mismatches by the hex system of Streptococcus pneumoniae was examined by comparison of the nucleotide sequence of the wild-type and eight mutant alleles of the malM gene. A detailed restriction map was constructed for pLS70, and the nucleotide sequence was determined for...


    pub_type: 杂志文章


    authors: Lacks SA,Dunn JJ,Greenberg B

    更新日期:1982-12-01 00:00:00

  • Transcription of specific genes in isolated nuclei by exogenous RNA polymerases.

    abstract::Mouse plasmacytoma (MOPC) 460 cells contain two chromatographic forms of RNA polymerase III (IIIA and IIIB) in addition to the major class I and II RNA polymerases. Nuclei isolated from these cells actively synthesize RNA. Among the discrete transcription products observed are the 5S and 4.5S RNAs and additional low m...


    pub_type: 杂志文章


    authors: Sklar VE,Roeder RG

    更新日期:1977-03-01 00:00:00

  • Deubiquitinating enzyme Ubp6 functions noncatalytically to delay proteasomal degradation.

    abstract::Ubiquitin chains serve as a recognition motif for the proteasome, a multisubunit protease, which degrades its substrates into polypeptides while releasing ubiquitin for reuse. Yeast proteasomes contain two deubiquitinating enzymes, Ubp6 and Rpn11. Rpn11 promotes protein breakdown through its degradation-coupled activi...


    pub_type: 杂志文章


    authors: Hanna J,Hathaway NA,Tone Y,Crosas B,Elsasser S,Kirkpatrick DS,Leggett DS,Gygi SP,King RW,Finley D

    更新日期:2006-10-06 00:00:00

  • GroEL/GroES-mediated folding of a protein too large to be encapsulated.

    abstract::The chaperonin GroEL binds nonnative proteins too large to fit inside the productive GroEL-GroES cis cavity, but whether and how it assists their folding has remained unanswered. We have examined yeast mitochondrial aconitase, an 82 kDa monomeric Fe(4)S(4) cluster-containing enzyme, observed to aggregate in chaperonin...


    pub_type: 杂志文章


    authors: Chaudhuri TK,Farr GW,Fenton WA,Rospert S,Horwich AL

    更新日期:2001-10-19 00:00:00

  • Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.

    abstract::In many organisms, meiotic chromosomes are bundled at their telomeres to form a "bouquet" arrangement. The bouquet formation plays an important role in homologous chromosome pairing and therefore progression of meiosis. As meiotic telomere clustering occurs in response to mating pheromone signaling in fission yeast, w...


    pub_type: 杂志文章


    authors: Chikashige Y,Tsutsumi C,Yamane M,Okamasa K,Haraguchi T,Hiraoka Y

    更新日期:2006-04-07 00:00:00

  • Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2mu circle.

    abstract::We have identified two major and approximately ten minor poly(A)-containing RNA species in S. cerevisiae which arise from in vivo transcription of the yeast plasmid, known as 2mu circle. The two major species, which are 1325 and 1275 bases in length, are transcribed from the two unique halves of the plasmid and extend...


    pub_type: 杂志文章


    authors: Broach JR,Atkins JF,McGill C,Chow L

    更新日期:1979-04-01 00:00:00

  • Influence of metabolism on epigenetics and disease.

    abstract::Chemical modifications of histones and DNA, such as histone methylation, histone acetylation, and DNA methylation, play critical roles in epigenetic gene regulation. Many of the enzymes that add or remove such chemical modifications are known, or might be suspected, to be sensitive to changes in intracellular metaboli...


    pub_type: 杂志文章,评审


    authors: Kaelin WG Jr,McKnight SL

    更新日期:2013-03-28 00:00:00

  • A mouse homolog to the avian sarcoma virus src protein is a member of a protein kinase cascade.

    abstract::Recent work has identified a cascade of membrane bound protein kinases in Ehrlich ascites tumor cells. These enzymes, designated PKL, PKS and PKM, are present in both Ehrlich tumor and mouse brain, but the cascade is active only in the tumor tissue. We have now purified a fourth protein kinase, PKF, that is also assoc...


    pub_type: 杂志文章


    authors: Spector M,Pepinsky RB,Vogt VM,Racker E

    更新日期:1981-07-01 00:00:00

  • Premature of chromosome condensation in a ts DNA- mutant of BHK cells.

    abstract::A temperature-sensitive mutant of BHK, designated ts BN-2, shows a rapid drop in 3H-thymidine incorporation along with accumulation of the cells in the G1 phase of the cycle when asynchronous cultures are shifted from 33.5 degrees C to the nonpermissive temperature of 39.5 degrees C. Synchronized cultures of ts BN-2 c...


    pub_type: 杂志文章


    authors: Nishimoto T,Eilen E,Basilico C

    更新日期:1978-10-01 00:00:00

  • Organoid cultures derived from patients with advanced prostate cancer.

    abstract::The lack of in vitro prostate cancer models that recapitulate the diversity of human prostate cancer has hampered progress in understanding disease pathogenesis and therapy response. Using a 3D organoid system, we report success in long-term culture of prostate cancer from biopsy specimens and circulating tumor cells....


    pub_type: 杂志文章


    authors: Gao D,Vela I,Sboner A,Iaquinta PJ,Karthaus WR,Gopalan A,Dowling C,Wanjala JN,Undvall EA,Arora VK,Wongvipat J,Kossai M,Ramazanoglu S,Barboza LP,Di W,Cao Z,Zhang QF,Sirota I,Ran L,MacDonald TY,Beltran H,Mosquera J

    更新日期:2014-09-25 00:00:00

  • Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation.

    abstract::The ligand for osteoprotegerin has been identified, and it is a TNF-related cytokine that replaces the requirement for stromal cells, vitamin D3, and glucocorticoids in the coculture model of in vitro osteoclastogenesis. OPG ligand (OPGL) binds to a unique hematopoeitic progenitor cell that is committed to the osteocl...


    pub_type: 杂志文章


    authors: Lacey DL,Timms E,Tan HL,Kelley MJ,Dunstan CR,Burgess T,Elliott R,Colombero A,Elliott G,Scully S,Hsu H,Sullivan J,Hawkins N,Davy E,Capparelli C,Eli A,Qian YX,Kaufman S,Sarosi I,Shalhoub V,Senaldi G,Guo J,Delane

    更新日期:1998-04-17 00:00:00

  • Adipose Tissue Macrophage-Derived Exosomal miRNAs Can Modulate In Vivo and In Vitro Insulin Sensitivity.

    abstract::MiRNAs are regulatory molecules that can be packaged into exosomes and secreted from cells. Here, we show that adipose tissue macrophages (ATMs) in obese mice secrete miRNA-containing exosomes (Exos), which cause glucose intolerance and insulin resistance when administered to lean mice. Conversely, ATM Exos obtained f...


    pub_type: 杂志文章


    authors: Ying W,Riopel M,Bandyopadhyay G,Dong Y,Birmingham A,Seo JB,Ofrecio JM,Wollam J,Hernandez-Carretero A,Fu W,Li P,Olefsky JM

    更新日期:2017-10-05 00:00:00