Abstract:
:DNA repair was measured in the dihydrofolate reductase gene in Chinese hamster ovary cells, amplified for the gene, by quantitating pyrimidine dimers with a specific UV-endonuclease. More than two thirds of the dimers had been removed from a 14.1 kb restriction fragment of the gene by 26 hr after irradiation (20 J/m2), while little removal was detected in fragments upstream of the gene and only 15% were removed from the genome overall. This suggests that damage processing can vary according to function or activity of affected sequences, which has general implications for correlations of DNA repair with survival and mutagenesis. Perhaps preferential repair of vital sequences facilitates UV-resistance of these cells despite low overall repair levels.
journal_name
Celljournal_title
Cellauthors
Bohr VA,Smith CA,Okumoto DS,Hanawalt PCdoi
10.1016/0092-8674(85)90150-3subject
Has Abstractpub_date
1985-02-01 00:00:00pages
359-69issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(85)90150-3journal_volume
40pub_type
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