Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA.


:Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova telomere end binding protein (OnTEBP) complexed with single strand telomeric DNA at 2.8 A resolution. The structure reveals four oligonucleotide/oligosaccharide-binding folds, three of which form a deep cleft that binds the ssDNA, and a fourth that forms an unusual protein-protein interaction between the alpha and beta subunits. This structure provides a molecular description of how the two subunits of OnTEBP recognize and bind ssDNA to form a sequence-specific, telomeric nucleoprotein complex that caps the very 3' ends of chromosomes.






Horvath MP,Schweiker VL,Bevilacqua JM,Ruggles JA,Schultz SC




Has Abstract


1998-12-23 00:00:00
















  • Binding of a high affinity phosphotyrosyl peptide to the Src SH2 domain: crystal structures of the complexed and peptide-free forms.

    abstract::The crystal structure of the Src SH2 domain complexed with a high affinity 11-residue phosphopeptide has been determined at 2.7 A resolution by X-ray diffraction. The peptide binds in an extended conformation and makes primary interactions with the SH2 domain at six central residues: PQ(pY)EEI. The phosphotyrosine and...


    pub_type: 杂志文章


    authors: Waksman G,Shoelson SE,Pant N,Cowburn D,Kuriyan J

    更新日期:1993-03-12 00:00:00

  • Viral defense: it takes two MAVS to Tango.

    abstract::To defend cells against viruses, the MAVS (mitochondrial antiviral signaling) adaptor protein initiates an antiviral signaling cascade from mitochondrial membranes. In this issue, Dixit et al. (2010) show that MAVS also localizes to the membranes of peroxisomes, where it rapidly induces expression of a subset of antiv...


    pub_type: 评论,杂志文章


    authors: Sharma S,Fitzgerald KA

    更新日期:2010-05-14 00:00:00

  • The lta4h locus modulates susceptibility to mycobacterial infection in zebrafish and humans.

    abstract::Exposure to Mycobacterium tuberculosis produces varied early outcomes, ranging from resistance to infection to progressive disease. Here we report results from a forward genetic screen in zebrafish larvae that identify multiple mutant classes with distinct patterns of innate susceptibility to Mycobacterium marinum. A ...


    pub_type: 杂志文章


    authors: Tobin DM,Vary JC Jr,Ray JP,Walsh GS,Dunstan SJ,Bang ND,Hagge DA,Khadge S,King MC,Hawn TR,Moens CB,Ramakrishnan L

    更新日期:2010-03-05 00:00:00

  • Genomic and Transcriptomic Features of Response to Anti-PD-1 Therapy in Metastatic Melanoma.

    abstract::PD-1 immune checkpoint blockade provides significant clinical benefits for melanoma patients. We analyzed the somatic mutanomes and transcriptomes of pretreatment melanoma biopsies to identify factors that may influence innate sensitivity or resistance to anti-PD-1 therapy. We find that overall high mutational loads a...


    pub_type: 评论,杂志文章


    authors: Hugo W,Zaretsky JM,Sun L,Song C,Moreno BH,Hu-Lieskovan S,Berent-Maoz B,Pang J,Chmielowski B,Cherry G,Seja E,Lomeli S,Kong X,Kelley MC,Sosman JA,Johnson DB,Ribas A,Lo RS

    更新日期:2016-03-24 00:00:00

  • Hedgehog controls limb development by regulating the activities of distinct transcriptional activator and repressor forms of Cubitus interruptus.

    abstract::Hedgehog (Hh) proteins play diverse organizing roles in development by regulating gene expression in responding cells. The Gli homolog Cubitus interruptus (Ci) is involved in controlling the transcription of Hh target genes. A repressor form of Ci arises in the absence of Hh signaling by proteolytic cleavage of intact...


    pub_type: 杂志文章


    authors: Méthot N,Basler K

    更新日期:1999-03-19 00:00:00

  • Structure of the l(2)gl gene of Drosophila and delimitation of its tumor suppressor domain.

    abstract::We have previously cloned lethal(2)giant larvae, a tumor-suppressor gene of Drosophila that normally controls cell proliferation and/or differentiation in the optic centers of the brain and the imaginal discs. Here we describe the structure of the l(2)gl genes as determined by sequencing genomic and cDNA clones. The s...


    pub_type: 杂志文章


    authors: Jacob L,Opper M,Metzroth B,Phannavong B,Mechler BM

    更新日期:1987-07-17 00:00:00

  • The membrane-spanning segment of invariant chain (I gamma) contains a potentially cleavable signal sequence.

    abstract::The human invariant chain (I gamma) of class II histocompatibility antigens spans the membrane of the endoplasmic reticulum once. It exposes a small amino-terminal domain on the cytoplasmic side and a carboxy-terminal, glycosylated domain on the exoplasmic side of the membrane. When the exoplasmic domain of I gamma is...


    pub_type: 杂志文章


    authors: Lipp J,Dobberstein B

    更新日期:1986-09-26 00:00:00

  • A Quantitative Proteome Map of the Human Body.

    abstract::Determining protein levels in each tissue and how they compare with RNA levels is important for understanding human biology and disease as well as regulatory processes that control protein levels. We quantified the relative protein levels from over 12,000 genes across 32 normal human tissues. Tissue-specific or tissue...


    pub_type: 杂志文章


    authors: Jiang L,Wang M,Lin S,Jian R,Li X,Chan J,Dong G,Fang H,Robinson AE,GTEx Consortium.,Snyder MP

    更新日期:2020-10-01 00:00:00

  • Analysis of a spatially regulated phosphotyrosine phosphatase identifies tyrosine phosphorylation as a key regulatory pathway in Dictyostelium.

    abstract::We have cloned a Dictyostelium phosphotyrosine phosphatase (PTP1) with a catalytic domain showing approximately 38%-50% amino acid identity to those of other PTPs. PTP1 contains an approximately 99 amino acid insert and bacterially produced PTP1 possesses PTP activity. PTP1 is expressed at a very low level in vegetati...


    pub_type: 杂志文章


    authors: Howard PK,Sefton BM,Firtel RA

    更新日期:1992-11-13 00:00:00

  • Molecular cloning of cDNA for human von Willebrand factor: authentication by a new method.

    abstract::We have identified a 2.4 kb partial cDNA clone (pDL34) for human von Willebrand factor (vWf) mRNA. pDL34 was selected by screening an endothelial cell cDNA library with a radiolabeled reverse transcript of mRNA obtained by specific immunoisolation of vWf polysomes from endothelial cells. pDL34 selectively hybridized t...


    pub_type: 杂志文章


    authors: Lynch DC,Zimmerman TS,Collins CJ,Brown M,Morin MJ,Ling EH,Livingston DM

    更新日期:1985-05-01 00:00:00

  • Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.

    abstract::Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA sequencing to examine ventral midbrain development in human and ...


    pub_type: 杂志文章


    authors: La Manno G,Gyllborg D,Codeluppi S,Nishimura K,Salto C,Zeisel A,Borm LE,Stott SRW,Toledo EM,Villaescusa JC,Lönnerberg P,Ryge J,Barker RA,Arenas E,Linnarsson S

    更新日期:2016-10-06 00:00:00

  • Postnatal development of a demyelinating disease in avian spinal cord chimeras.

    abstract::Xenogeneic spinal cord chimeras were constructed by grafting fragments of quail neural primordium into chick embryos at 2 days of incubation. Hatched birds displayed normal motor behavior for about 5 to 7 weeks, whereupon they developed a neurological syndrome; in the grafted spinal cord the pathological signs of the ...


    pub_type: 杂志文章


    authors: Kinutani M,Coltey M,Le Douarin NM

    更新日期:1986-04-25 00:00:00

  • The Implication of Early Chromatin Changes in X Chromosome Inactivation.

    abstract::During development, the precise relationships between transcription and chromatin modifications often remain unclear. We use the X chromosome inactivation (XCI) paradigm to explore the implication of chromatin changes in gene silencing. Using female mouse embryonic stem cells, we initiate XCI by inducing Xist and then...


    pub_type: 杂志文章


    authors: Żylicz JJ,Bousard A,Žumer K,Dossin F,Mohammad E,da Rocha ST,Schwalb B,Syx L,Dingli F,Loew D,Cramer P,Heard E

    更新日期:2019-01-10 00:00:00

  • Sequence analysis and neuronal expression of fasciclin I in grasshopper and Drosophila.

    abstract::The fasciclin I, II, and III glycoproteins are expressed on different subsets of axon bundles (fascicles) in insect embryos and are thus candidates for surface recognition molecules involved in growth cone guidance. Here we present the sequence of grasshopper fasciclin I and the identification and sequence of the Dros...


    pub_type: 杂志文章


    authors: Zinn K,McAllister L,Goodman CS

    更新日期:1988-05-20 00:00:00

  • The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation.

    abstract::The DDB1-CUL4-RBX1 (CRL4) ubiquitin ligase family regulates a diverse set of cellular pathways through dedicated substrate receptors (DCAFs). The DCAF DDB2 detects UV-induced pyrimidine dimers in the genome and facilitates nucleotide excision repair. We provide the molecular basis for DDB2 receptor-mediated cyclobutan...


    pub_type: 杂志文章


    authors: Fischer ES,Scrima A,Böhm K,Matsumoto S,Lingaraju GM,Faty M,Yasuda T,Cavadini S,Wakasugi M,Hanaoka F,Iwai S,Gut H,Sugasawa K,Thomä NH

    更新日期:2011-11-23 00:00:00

  • Intrinsic activity of the Lin-12 and Notch intracellular domains in vivo.

    abstract::The lin-12 gene of C. elegans and the Notch gene of D. melanogaster encode structurally related transmembrane proteins that mediate intercellular signaling. We show that truncated forms of these proteins consisting of only the intracellular domains cause cell fate transformations associated with constitutive activity ...


    pub_type: 杂志文章


    authors: Struhl G,Fitzgerald K,Greenwald I

    更新日期:1993-07-30 00:00:00

  • The many faces of PPARgamma.

    abstract::In an era marked by the increasing prevalence of obesity, diabetes, and cardiovascular disease, the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARgamma) has emerged as a transcriptional regulator of metabolism whose activity can be modulated by direct binding of small molecules. As the master ...


    pub_type: 杂志文章,评审


    authors: Lehrke M,Lazar MA

    更新日期:2005-12-16 00:00:00

  • Reducing the mystery of neuronal differentiation.

    abstract::In the developing nervous system, neural progenitors exit the cell cycle and differentiate on a precise schedule, yet the mechanisms driving this process remain poorly defined. Yan et al. (2009) now identify a thiol-redox reaction mediated by the membrane protein GDE2 and the peroxiredoxin protein Prdx1 that promotes ...


    pub_type: 评论,杂志文章


    authors: Novitch BG,Butler SJ

    更新日期:2009-09-18 00:00:00

  • RIPK1 blocks early postnatal lethality mediated by caspase-8 and RIPK3.

    abstract::Receptor-interacting protein kinase (RIPK)-1 is involved in RIPK3-dependent and -independent signaling pathways leading to cell death and/or inflammation. Genetic ablation of ripk1 causes postnatal lethality, which was not prevented by deletion of ripk3, caspase-8, or fadd. However, animals that lack RIPK1, RIPK3, and...


    pub_type: 杂志文章


    authors: Dillon CP,Weinlich R,Rodriguez DA,Cripps JG,Quarato G,Gurung P,Verbist KC,Brewer TL,Llambi F,Gong YN,Janke LJ,Kelliher MA,Kanneganti TD,Green DR

    更新日期:2014-05-22 00:00:00

  • Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells.

    abstract::Subcellular compartments in which folding and assembly of proteins occur seem to have a set of PCB proteins capable of mediating these and related processes, such as translocation across membranes. When a domain of a polypeptide chain emerges from a ribosome during synthesis or from the distal side of a membrane durin...


    pub_type: 杂志文章,评审


    authors: Rothman JE

    更新日期:1989-11-17 00:00:00

  • Acetylation-dependent signal transduction for type I interferon receptor.

    abstract::Cytokine-activated receptors initiate intracellular signaling by recruiting protein kinases that phosphorylate the receptors on tyrosine residues, thus enabling docking of SH2 domain-bearing activating factors. Here we report that in response to type 1 interferon (IFNalpha), IFNalpha receptors recruit cytoplasmic CREB...


    pub_type: 杂志文章


    authors: Tang X,Gao JS,Guan YJ,McLane KE,Yuan ZL,Ramratnam B,Chin YE

    更新日期:2007-10-05 00:00:00

  • Cooperativity in transactivation between retinoic acid receptor and TFIID requires an activity analogous to E1A.

    abstract::In embryonal carcinoma (EC) cells retinoic acid (RA) strongly induces transcription from the RA receptor beta 2 (RAR beta 2) promoter through an RA response element (RARE) located in close proximity to the TATA box. Here we demonstrate that recombinant human TATA box-binding protein, hTFIID, and RAR functionally coope...


    pub_type: 杂志文章


    authors: Berkenstam A,Vivanco Ruiz MM,Barettino D,Horikoshi M,Stunnenberg HG

    更新日期:1992-05-01 00:00:00

  • Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion.

    abstract::We describe genetic and physical characterization of rearrangements of chromosome III which result in changes of cell type in S. cerevisiae. Two types of rearrangements were obtained as rare events which caused a change at the locus controlling cell type, MAT, associated with a recessive lethal mutation, in one case f...


    pub_type: 杂志文章


    authors: Strathern JN,Newlon CS,Herskowitz I,Hicks JB

    更新日期:1979-10-01 00:00:00

  • Preclinical mouse cancer models: a maze of opportunities and challenges.

    abstract::Significant advances have been made in developing novel therapeutics for cancer treatment, and targeted therapies have revolutionized the treatment of some cancers. Despite the promise, only about five percent of new cancer drugs are approved, and most fail due to lack of efficacy. The indication is that current precl...


    pub_type: 杂志文章,评审


    authors: Day CP,Merlino G,Van Dyke T

    更新日期:2015-09-24 00:00:00

  • A Drosophila neurexin is required for septate junction and blood-nerve barrier formation and function.

    abstract::Septate and tight junctions are thought to seal neighboring cells together and to function as barriers between epithelial cells. We have characterized a novel member of the neurexin family, Neurexin IV (NRX), which is localized to septate junctions (SJs) of epithelial and glial cells. NRX is a transmembrane protein wi...


    pub_type: 杂志文章


    authors: Baumgartner S,Littleton JT,Broadie K,Bhat MA,Harbecke R,Lengyel JA,Chiquet-Ehrismann R,Prokop A,Bellen HJ

    更新日期:1996-12-13 00:00:00

  • Direct repeats flank three small nuclear RNA pseudogenes in the human genome.

    abstract::We previously demonstrated that pseudogenes complementary to the small nuclear RNAs U1, U2 and U3 are dispersed and abundant in the human genome. Here we report that three pseudogenes (U1.101, U2.13 and U3.5) are flanked by perfect short direct repeats of 16 to 19 base pairs. In all three pseudogenes. the upstream dir...


    pub_type: 杂志文章


    authors: Van Arsdell SW,Denison RA,Bernstein LB,Weiner AM,Manser T,Gesteland RF

    更新日期:1981-10-01 00:00:00

  • A neural circuit mechanism integrating motivational state with memory expression in Drosophila.

    abstract::Behavioral expression of food-associated memory in fruit flies is constrained by satiety and promoted by hunger, suggesting an influence of motivational state. Here, we identify a neural mechanism that integrates the internal state of hunger and appetitive memory. We show that stimulation of neurons that express neuro...


    pub_type: 杂志文章


    authors: Krashes MJ,DasGupta S,Vreede A,White B,Armstrong JD,Waddell S

    更新日期:2009-10-16 00:00:00

  • Our Gut Microbiome: The Evolving Inner Self.

    abstract::The "holobiont" concept, defined as the collective contribution of the eukaryotic and prokaryotic counterparts to the multicellular organism, introduces a complex definition of individuality enabling a new comprehensive view of human evolution and personalized characteristics. Here, we provide snapshots of the evolvin...


    pub_type: 杂志文章,评审


    authors: Kundu P,Blacher E,Elinav E,Pettersson S

    更新日期:2017-12-14 00:00:00

  • Regulatory Expansion in Mammals of Multivalent hnRNP Assemblies that Globally Control Alternative Splicing.

    abstract::Alternative splicing (AS) patterns have diverged rapidly during vertebrate evolution, yet the functions of most species- and lineage-specific splicing events are not known. We observe that mammalian-specific AS events are enriched in transcript sequences encoding intrinsically disordered regions (IDRs) of proteins, in...


    pub_type: 杂志文章


    authors: Gueroussov S,Weatheritt RJ,O'Hanlon D,Lin ZY,Narula A,Gingras AC,Blencowe BJ

    更新日期:2017-07-13 00:00:00

  • Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome.

    abstract::Pathogenic simian immunodeficiency virus (SIV) infection is associated with enteropathy, which likely contributes to AIDS progression. To identify candidate etiologies for AIDS enteropathy, we used next-generation sequencing to define the enteric virome during SIV infection in nonhuman primates. Pathogenic, but not no...


    pub_type: 杂志文章


    authors: Handley SA,Thackray LB,Zhao G,Presti R,Miller AD,Droit L,Abbink P,Maxfield LF,Kambal A,Duan E,Stanley K,Kramer J,Macri SC,Permar SR,Schmitz JE,Mansfield K,Brenchley JM,Veazey RS,Stappenbeck TS,Wang D,Barouch DH,

    更新日期:2012-10-12 00:00:00