当前位置： SCI文献检索 > CELL期刊下所有文献 > Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA.

Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA.

Abstract：

:Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova telomere end binding protein (OnTEBP) complexed with single strand telomeric DNA at 2.8 A resolution. The structure reveals four oligonucleotide/oligosaccharide-binding folds, three of which form a deep cleft that binds the ssDNA, and a fourth that forms an unusual protein-protein interaction between the alpha and beta subunits. This structure provides a molecular description of how the two subunits of OnTEBP recognize and bind ssDNA to form a sequence-specific, telomeric nucleoprotein complex that caps the very 3' ends of chromosomes.

journal_name

Cell

journal_title

Cell

authors

Horvath MP,Schweiker VL,Bevilacqua JM,Ruggles JA,Schultz SC

doi

10.1016/s0092-8674(00)81720-1

subject

Has Abstract

pub_date

1998-12-23 00:00:00

pages

963-74

issue

7

eissn

0092-8674

issn

1097-4172

pii

S0092-8674(00)81720-1

journal_volume

95

pub_type

杂志文章

相关文献

CELL文献大全
  • Growth-rate-dependent regulation of ribosome synthesis in E. coli: expression of the lacZ and galK genes fused to ribosomal promoters.

    abstract：:Hybrid transducing phages were constructed in vitro that carry the galK gene fused to each of three ribosomal promoters: the promotor for an rRNA operon (rrnE); the promoter for the spec r protein operon and the promotor for the alpha r protein operon. We also constructed hybrid transducing phages that carry the IacZ ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(81)90185-9

    authors： Miura A,Krueger JH,Itoh S,de Boer HA,Nomura M

    更新日期：1981-09-01 00:00:00

  • A role for casein kinase 2 in the mechanism underlying circadian temperature compensation.

    abstract：:Temperature compensation of circadian clocks is an unsolved problem with relevance to the general phenomenon of biological compensation. We identify casein kinase 2 (CK2) as a key regulator of temperature compensation of the Neurospora clock by determining that two long-standing clock mutants, chrono and period-3, dis...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2009.03.019

    authors： Mehra A,Shi M,Baker CL,Colot HV,Loros JJ,Dunlap JC

    更新日期：2009-05-15 00:00:00

  • ATR-X syndrome protein targets tandem repeats and influences allele-specific expression in a size-dependent manner.

    abstract：:ATRX is an X-linked gene of the SWI/SNF family, mutations in which cause syndromal mental retardation and downregulation of α-globin expression. Here we show that ATRX binds to tandem repeat (TR) sequences in both telomeres and euchromatin. Genes associated with these TRs can be dysregulated when ATRX is mutated, and ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2010.09.023

    authors： Law MJ,Lower KM,Voon HP,Hughes JR,Garrick D,Viprakasit V,Mitson M,De Gobbi M,Marra M,Morris A,Abbott A,Wilder SP,Taylor S,Santos GM,Cross J,Ayyub H,Jones S,Ragoussis J,Rhodes D,Dunham I,Higgs DR,Gibbons RJ

    更新日期：2010-10-29 00:00:00

  • Temporal regulation of salmonella virulence effector function by proteasome-dependent protein degradation.

    abstract：:Salmonella enterica invasion of host cells requires the reversible activation of the Rho-family GTPases Cdc42 and Rac1 by the bacterially encoded GEF SopE and the GAP SptP, which exert their function at different times during infection and are delivered into host cells by a type III secretion system. We found that Sop...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(03)00849-3

    authors： Kubori T,Galán JE

    更新日期：2003-10-31 00:00:00

  • Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes.

    abstract：:It has been long appreciated that, during meiosis, DNA replication is coordinated with the subsequent formation of the double-strand breaks (DSBs) that initiate recombination, but a mechanistic understanding of this process was elusive. We now show that, in yeast, the replisome-associated components Tof1 and Csm3 phys...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2014.06.028

    authors： Murakami H,Keeney S

    更新日期：2014-08-14 00:00:00

  • Regulation of the microtubule steady state in vitro by ATP.

    abstract：:ATP increases microtubule steady state assembly and disassembly rates in vitro in a concentration-dependent manner. Bovine brain microtubules, composed of 75% tubulin and 25% high molecular weight microtubule-associated proteins (MAPs), were purified by three cycles of assembly and disassembly in the absence of ATP. W...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(79)90122-3

    authors： Margolis RL,Wilson L

    更新日期：1979-11-01 00:00:00

  • Targeted disruption of the trkB neurotrophin receptor gene results in nervous system lesions and neonatal death.

    abstract：:We have generated mice carrying a germline mutation in the tyrosine kinase catalytic domain of the trkB gene. This mutation eliminates expression of gp145trkB, a protein-tyrosine kinase that serves as the signaling receptor for two members of the nerve growth factor family of neurotrophins, brain-derived neurotrophic ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：

    authors： Klein R,Smeyne RJ,Wurst W,Long LK,Auerbach BA,Joyner AL,Barbacid M

    更新日期：1993-10-08 00:00:00

  • An mRNA maturase is encoded by the first intron of the mitochondrial gene for the subunit I of cytochrome oxidase in S. cerevisiae.

    abstract：:We have localized ten oxi3- mutations in the first, al1, intron of the coxl gene. All are splicing deficient, being unable to excise the intron. Complementation experiments disclose several domains in the intron al1: the 5'-proximal and 3'-proximal domains harbor cis-dominant mutations, while trans-recessive ones are ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(83)90106-x

    authors： Carignani G,Groudinsky O,Frezza D,Schiavon E,Bergantino E,Slonimski PP

    更新日期：1983-12-01 00:00:00

  • The primary structure of the putative oncogene pim-1 shows extensive homology with protein kinases.

    abstract：:We have shown previously that the putative oncogene pim-1 is frequently activated by provirus insertion in murine leukemia virus-induced T cell lymphomas. Here we describe the structure of the pim-1 gene as determined by sequencing genomic and cDNA clones. The gene has an open reading frame, encoding a protein of 313 ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(86)90886-x

    authors： Selten G,Cuypers HT,Boelens W,Robanus-Maandag E,Verbeek J,Domen J,van Beveren C,Berns A

    更新日期：1986-08-15 00:00:00

  • Could iPSCs Enable "Off-the-Shelf" Cell Therapy?

    abstract：:An "off-the-shelf" cell therapy derived from induced pluripotent stem cells (iPSCs) has entered clinical trials in the United States. Other companies are following suit, harnessing iPSCs' self-renewal ability to manufacture cell therapies that don't require customization for each patient. But some experts aren't sure ...

    journal_title：Cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.cell.2019.05.043

    authors： Crow D

    更新日期：2019-06-13 00:00:00

  • Overproduction of the Tn3 transposition protein and its role in DNA transposition.

    abstract：:Five single base pair mutations that increase expression of the tnpA (transposase) gene of the Tn3 transposon approximately 30-fold, but which still allow the gene to be regulated, have been isolated by using a generally applicable procedure that involves distally linked lac gene fusions. The mutations, which are all ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(82)90352-x

    authors： Casadaban MJ,Chou J,Cohen SN

    更新日期：1982-02-01 00:00:00

  • Transcription factor dosage affects changes in higher order chromatin structure associated with activation of a heterochromatic gene.

    abstract：:The mechanisms of transcriptional activation in heterochromatin were investigated by using FISH to directly visualize changes in chromatin organization during activation of a heterochromatic lambda5 transgene. A DNase I hypersensitive site was shown to relocate the transgene to the outside of the pericentromeric heter...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)00177-x

    authors： Lundgren M,Chow CM,Sabbattini P,Georgiou A,Minaee S,Dillon N

    更新日期：2000-11-22 00:00:00

  • Crystal Structure of Antagonist Bound Human Lysophosphatidic Acid Receptor 1.

    abstract：:Lipid biology continues to emerge as an area of significant therapeutic interest, particularly as the result of an enhanced understanding of the wealth of signaling molecules with diverse physiological properties. This growth in knowledge is epitomized by lysophosphatidic acid (LPA), which functions through interactio...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2015.06.002

    authors： Chrencik JE,Roth CB,Terakado M,Kurata H,Omi R,Kihara Y,Warshaviak D,Nakade S,Asmar-Rovira G,Mileni M,Mizuno H,Griffith MT,Rodgers C,Han GW,Velasquez J,Chun J,Stevens RC,Hanson MA

    更新日期：2015-06-18 00:00:00

  • Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.

    abstract：:NF-kappa B is a nuclear protein, found only in cells that transcribe immunoglobulin light chain genes, that interacts with a defined site in the kappa immunoglobulin enhancer. This protein can be induced in pre-B cells by stimulation with bacterial lipopolysaccharide (LPS). The induction involves a posttranslational a...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(86)90807-x

    authors： Sen R,Baltimore D

    更新日期：1986-12-26 00:00:00

  • Adipocyte NCoR knockout decreases PPARγ phosphorylation and enhances PPARγ activity and insulin sensitivity.

    abstract：:Insulin resistance, tissue inflammation, and adipose tissue dysfunction are features of obesity and Type 2 diabetes. We generated adipocyte-specific Nuclear Receptor Corepressor (NCoR) knockout (AKO) mice to investigate the function of NCoR in adipocyte biology, glucose and insulin homeostasis. Despite increased obesi...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2011.09.050

    authors： Li P,Fan W,Xu J,Lu M,Yamamoto H,Auwerx J,Sears DD,Talukdar S,Oh D,Chen A,Bandyopadhyay G,Scadeng M,Ofrecio JM,Nalbandian S,Olefsky JM

    更新日期：2011-11-11 00:00:00

  • Structural analysis of autoinhibition in the Ras activator Son of sevenless.

    abstract：:The classical model for the activation of the nucleotide exchange factor Son of sevenless (SOS) involves its recruitment to the membrane, where it engages Ras. The recent discovery that Ras*GTP is an allosteric activator of SOS indicated that the regulation of SOS is more complex than originally envisaged. We now pres...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2004.10.005

    authors： Sondermann H,Soisson SM,Boykevisch S,Yang SS,Bar-Sagi D,Kuriyan J

    更新日期：2004-10-29 00:00:00

  • Transmembrane location of oligosaccharide-lipid synthesis in microsomal vesicles.

    abstract：:The oligosaccharide-lipid which is the precursor of asparagine-linked oligosaccharides of eucaryotic glycoproteins is synthesized from sugar nucleotides in the endoplasmic reticulum. The transmembrane location of the assembly of this oligosaccharide-lipid has been studied in vitro in rat liver microsomes. Protease tre...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(80)90475-4

    authors： Snider MD,Sultzman LA,Robbins PW

    更新日期：1980-09-01 00:00:00

  • Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

    abstract：:Conversion of glial cells into functional neurons represents a potential therapeutic approach for replenishing neuronal loss associated with neurodegenerative diseases and brain injury. Previous attempts in this area using expression of transcription factors were hindered by the low conversion efficiency and failure o...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2020.03.024

    authors： Zhou H,Su J,Hu X,Zhou C,Li H,Chen Z,Xiao Q,Wang B,Wu W,Sun Y,Zhou Y,Tang C,Liu F,Wang L,Feng C,Liu M,Li S,Zhang Y,Xu H,Yao H,Shi L,Yang H

    更新日期：2020-04-30 00:00:00

  • The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila.

    abstract：:Two independent approaches to understanding the molecular mechanism of cytokinesis have converged on the gene spaghetti-squash (sqh). A genetic screen for mitotic mutants identified sqh1, a mutation that disrupts cytokinesis, which was then cloned by transposon tagging. Independently, the gene that encodes the regulat...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(91)90013-o

    authors： Karess RE,Chang XJ,Edwards KA,Kulkarni S,Aguilera I,Kiehart DP

    更新日期：1991-06-28 00:00:00

  • The Drosophila single-minded gene encodes a nuclear protein with sequence similarity to the per gene product.

    abstract：:Mutations in the single-minded (sim) gene of Drosophila result in the loss of the precursor cells giving rise to the midline cells of the embryonic central nervous system. We have examined the structure of the sim product by sequencing a sim cDNA clone, and have also determined the subcellular localization of the prot...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(88)90538-7

    authors： Crews ST,Thomas JB,Goodman CS

    更新日期：1988-01-15 00:00:00

  • Asynchronous switching of flagellar motors on a single bacterial cell.

    abstract：:Salmonella possesses several flagella, each capable of counterclockwise and clockwise rotation. Counterclockwise rotation produces swimming, clockwise rotation produces tumbling. Switching between senses occurs stochastically. The rotational sense of individual flagella on a single cell could be monitored under specia...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(83)90501-9

    authors： Macnab RM,Han DP

    更新日期：1983-01-01 00:00:00

  • The GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21.

    abstract：:The neurofibromatosis type 1 (NF1) protein contains a region of significant sequence similarity to ras p21 GTPase-activating protein (GAP) and the yeast IRA1 gene product. A fragment of NF1 cDNA encoding the GAP-related domain (NF1 GRD) was expressed, immunoaffinity purified, and assayed for effects on N-ras p21 GTPas...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(90)90150-d

    authors： Martin GA,Viskochil D,Bollag G,McCabe PC,Crosier WJ,Haubruck H,Conroy L,Clark R,O'Connell P,Cawthon RM

    更新日期：1990-11-16 00:00:00

  • TGF-beta signaling specifies axons during brain development.

    abstract：:In the mammalian brain, the specification of a single axon and multiple dendrites occurs early in the differentiation of most neuron types. Numerous intracellular signaling events for axon specification have been described in detail. However, the identity of the extracellular factor(s) that initiate neuronal polarity ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2010.06.010

    authors： Yi JJ,Barnes AP,Hand R,Polleux F,Ehlers MD

    更新日期：2010-07-09 00:00:00

  • A Mild PUM1 Mutation Is Associated with Adult-Onset Ataxia, whereas Haploinsufficiency Causes Developmental Delay and Seizures.

    abstract：:Certain mutations can cause proteins to accumulate in neurons, leading to neurodegeneration. We recently showed, however, that upregulation of a wild-type protein, Ataxin1, caused by haploinsufficiency of its repressor, the RNA-binding protein Pumilio1 (PUM1), also causes neurodegeneration in mice. We therefore search...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2018.02.006

    authors： Gennarino VA,Palmer EE,McDonell LM,Wang L,Adamski CJ,Koire A,See L,Chen CA,Schaaf CP,Rosenfeld JA,Panzer JA,Moog U,Hao S,Bye A,Kirk EP,Stankiewicz P,Breman AM,McBride A,Kandula T,Dubbs HA,Macintosh R,Cardamone M

    更新日期：2018-02-22 00:00:00

  • Regulation of a late phase of T cell polarity and effector functions by Crtam.

    abstract：:Spatial organization of cellular proteins plays an important role in establishment of cellular polarity to regulate cell division, differentiation, migration, and organogenesis. Activation of T cells by antigen-presenting cells (APCs) results in the formation of an immunological synapse (IS), assembly of a signaling s...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2008.01.013

    authors： Yeh JH,Sidhu SS,Chan AC

    更新日期：2008-03-07 00:00:00

  • Influence of metabolism on epigenetics and disease.

    abstract：:Chemical modifications of histones and DNA, such as histone methylation, histone acetylation, and DNA methylation, play critical roles in epigenetic gene regulation. Many of the enzymes that add or remove such chemical modifications are known, or might be suspected, to be sensitive to changes in intracellular metaboli...

    journal_title：Cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.cell.2013.03.004

    authors： Kaelin WG Jr,McKnight SL

    更新日期：2013-03-28 00:00:00

  • Drosophila ribosomal RNA genes function as an X-Y pairing site during male meiosis.

    abstract：:In Drosophila melanogaster males, the sex chromosomes pair during meiosis in the centric X heterochromatin and at the base of the short arm of the Y (YS), in the vicinity of the nucleolus organizers. X chromosomes deficient for the pairing region segregate randomly from the Y. In this report we show that a single ribo...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(90)90215-z

    authors： McKee BD,Karpen GH

    更新日期：1990-04-06 00:00:00

  • Proline isomerization of histone H3 regulates lysine methylation and gene expression.

    abstract：:The cis-trans isomerization of proline serves as a regulatory switch in signaling pathways. We identify the proline isomerase Fpr4, a member of the FK506 binding protein family in Saccharomyces cerevisiae, as an enzyme which binds the amino-terminal tail of histones H3 and H4 and catalyses the isomerization of H3 prol...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2006.07.026

    authors： Nelson CJ,Santos-Rosa H,Kouzarides T

    更新日期：2006-09-08 00:00:00

  • Movement: How the Brain Communicates with the World.

    abstract：:Voluntary movement is a result of signals transmitted through a communication channel that links the internal world in our minds to the physical world around us. Intention can be considered the desire to effect change on our environment, and this is contained in the signals from the brain, passed through the nervous s...

    journal_title：Cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.cell.2016.02.038

    authors： Schwartz AB

    更新日期：2016-03-10 00:00:00

  • Intercellular nanotubes mediate bacterial communication.

    abstract：:Bacteria are known to communicate primarily via secreted extracellular factors. Here we identify a previously uncharacterized type of bacterial communication mediated by nanotubes that bridge neighboring cells. Using Bacillus subtilis as a model organism, we visualized transfer of cytoplasmic fluorescent molecules bet...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2011.01.015

    authors： Dubey GP,Ben-Yehuda S

    更新日期：2011-02-18 00:00:00