Abstract：
:The mechanisms of transcriptional activation in heterochromatin were investigated by using FISH to directly visualize changes in chromatin organization during activation of a heterochromatic lambda5 transgene. A DNase I hypersensitive site was shown to relocate the transgene to the outside of the pericentromeric heterochromatin complex in the absence of transcription. Activation of transcription, which is dependent on the transcription factor EBF, occurs in a stochastic manner that resembles telomeric silencing in yeast, with the transcribed gene remaining closely associated with the heterochromatin complex. Reducing the dosage of EBF results in a reduced frequency of localization of the transgene to the outside of the heterochromatin complex and lower levels of transcription. These data provide evidence that transcription factors can initiate changes in higher order chromatin structure during the earliest stages of gene activation.
journal_name
Celljournal_title
Cellauthors
Lundgren M,Chow CM,Sabbattini P,Georgiou A,Minaee S,Dillon Ndoi
10.1016/s0092-8674(00)00177-xsubject
Has Abstractpub_date
2000-11-22 00:00:00pages
733-43issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(00)00177-Xjournal_volume
103pub_type
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