Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma.

abstract：:DNA fragments containing the silencers that flank the mating type genes at HML alpha are shown to bind specifically to the nuclear scaffold of yeast. The scaffold proteins are solubilized with urea and then renatured to form a soluble extract which allows reconstitution of sequence-specific DNA loops. At the silent ma...

journal_title：Cell

authors： Hofmann JF,Laroche T,Brand AH,Gasser SM

更新日期：1989-06-02 00:00:00

abstract：:Clonal derivatives of C3HMT murine mammary cell lines in culture demonstrate conversion of mammary tumor virus (MMTV) expression at a rate of appriximately 6 per 100 clones. This alteration is largely unidirectional from a relatively high level (MMTV(H)) to a 10 fold lower level (MMTV(L)). This high rate of MMTV(L) va...

journal_title：Cell

authors： Parks WP,Hubbell ES,Goldberg RJ,O'Neill FJ,Scolnick EM

更新日期：1976-05-01 00:00:00

abstract：:Rubisco, the key enzyme of CO2 fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis...

journal_title：Cell

authors： Flecken M,Wang H,Popilka L,Hartl FU,Bracher A,Hayer-Hartl M

更新日期：2020-10-15 00:00:00

abstract：:Monkey cells synthesizing SV40 large T antigen were lysed and the extracts immunoprecipitated with either monoclonal anti-T antibody or monoclonal antibody to p110-114, the product of the retinoblastoma susceptibility gene (Rb). T and p110-114 coprecipitated in each case, implying that the proteins are complexed with ...

journal_title：Cell

authors： DeCaprio JA,Ludlow JW,Figge J,Shew JY,Huang CM,Lee WH,Marsilio E,Paucha E,Livingston DM

更新日期：1988-07-15 00:00:00

abstract：:Rapid flow of information in the nervous system involves presynaptic vesicle recycling by clathrin-mediated endocytosis, an event triggered by the alpha-adaptin-containing AP2 complex. We identified a Drosophila alpha-adaptin expressed in the garland cells, imaginal discs, and the CNS. Here we show its role in presyna...

journal_title：Cell

authors： González-Gaitán M,Jäckle H

更新日期：1997-03-21 00:00:00

abstract：:tDNA sequences essential for promotion of transcription have been identified for a tRNA1met gene of X. laevis. A cloned tRNA gene unit was altered by resection of the 5' flanking sequences or by specific deletions of gene and trailing sequences. Gene internal sequences were also substituted by unrelated sequences. The...

journal_title：Cell

authors： Hofstetter H,Kressman A,Birnstiel ML

更新日期：1981-05-01 00:00:00

abstract：:Mouse monoclonal antibody 4-I-244 detects a developmentally regulated antigen in embryos of Volvox carteri and inhibits specifically the morphogenetic process of inversion (the process by which the embryo turns inside out). Antigen 4-I-244 was chemically characterized as a complex phytosphingolipid containing the neut...

journal_title：Cell

authors： Wenzl S,Sumper M

更新日期：1986-08-15 00:00:00

abstract：:Human hepatitis B virus core protein expressed in E. coli assembles into two sizes of particle. We have determined their three-dimensional structures by electron cryomicroscopy and image processing. The large and small particles correspond to triangulation number T = 4 and T = 3 dimer clustered packings, containing 24...

journal_title：Cell

authors： Crowther RA,Kiselev NA,Böttcher B,Berriman JA,Borisova GP,Ose V,Pumpens P

更新日期：1994-06-17 00:00:00

abstract：:Recent studies suggest that local translation of mRNA within axons plays a key role in two aspects of growth cone navigation. One line of evidence implies a local synthesis of proteins that mediate growth cone collapse and turning in response to guidance cues; another indicates that growth cones possess the machinery ...

journal_title：Cell

authors： Steward O

更新日期：2002-09-06 00:00:00

abstract：:We have determined the complete 9202 nucleotide sequence of the visna lentivirus. The deduced genetic organization most closely resembles that of the AIDS retrovirus in that there is a novel central region separating pol and env. Moreover, there is a close phylogenetic relationship between the conserved reverse transc...

journal_title：Cell

authors： Sonigo P,Alizon M,Staskus K,Klatzmann D,Cole S,Danos O,Retzel E,Tiollais P,Haase A,Wain-Hobson S

更新日期：1985-08-01 00:00:00

abstract：:The Viviparous-1 (Vp1) gene of maize is specifically required for expression of the maturation program in seed development. We show that Vp1 encodes a 73,335 dalton protein with no detectable homology to known proteins. An acidic transcriptional activation sequence was identified by fusion to the GAL4 DNA-binding doma...

journal_title：Cell

authors： McCarty DR,Hattori T,Carson CB,Vasil V,Lazar M,Vasil IK

更新日期：1991-09-06 00:00:00

abstract：:Drosophila chorion genes are located in two clusters, one on the X chromosome at 7F1-2 of the polytene chromosome map and a second on the third chromosome at 66D11-15. Genes in both regions undergo amplification in ovarian follicle cells prior to their expression late in oogenesis. Analysis of cloned genomic segments ...

journal_title：Cell

authors： Spradling AC

更新日期：1981-11-01 00:00:00

abstract：:H2S is an endogenous gasotransmitter that plays an important role in physiological conditions. In this issue, Das et al. provide evidence that SIRT1-dependent angiogenesis is augmented by H2S-findings reinforced by Longchamp et al., who demonstrate that H2S-dependent angiogenesis is triggered by amino acid deprivation...

journal_title：Cell

authors： Arumugam TV,Kennedy BK

更新日期：2018-03-22 00:00:00

abstract：:Muscle cell cultures from Drosophila melanogaster were obtained by plating dissociated gastrula stage embryo cells on protamine-treated culture dishes. They myogenic cells in these cultures fuse to form multinucleated pulsating cells by 15 hr after plating. An analysis of protein synthesis during myogenesis in these c...

journal_title：Cell

authors： Storti RV,Horovitch SJ,Scott MP,Rich A,Pardue ML

更新日期：1978-04-01 00:00:00

abstract：:A safe and controlled manipulation of endocytosis in vivo may have disruptive therapeutic potential. Here, we demonstrate that the anti-emetic/anti-psychotic prochlorperazine can be repurposed to reversibly inhibit the in vivo endocytosis of membrane proteins targeted by therapeutic monoclonal antibodies, as directly ...

journal_title：Cell

authors： Chew HY,De Lima PO,Gonzalez Cruz JL,Banushi B,Echejoh G,Hu L,Joseph SR,Lum B,Rae J,O'Donnell JS,Merida de Long L,Okano S,King B,Barry R,Moi D,Mazzieri R,Thomas R,Souza-Fonseca-Guimaraes F,Foote M,McCluskey A,Robin

更新日期：2020-03-05 00:00:00

abstract：:Molecular chaperones assist protein folding by facilitating their "forward" folding and preventing aggregation. However, once aggregates have formed, these chaperones cannot facilitate protein disaggregation. Bacterial ClpB and its eukaryotic homolog Hsp104 are essential proteins of the heat-shock response, which have...

journal_title：Cell

authors： Lee S,Sowa ME,Watanabe YH,Sigler PB,Chiu W,Yoshida M,Tsai FT

更新日期：2003-10-17 00:00:00

abstract：:New biological tools provide new techniques to probe fundamental biological processes. Here we describe the burgeoning field of proteolysis-targeting chimeras (PROTACs), which are capable of modulating protein concentrations at a post-translational level by co-opting the ubiquitin-proteasome system. We describe the PR...

journal_title：Cell

authors： Burslem GM,Crews CM

更新日期：2020-04-02 00:00:00

abstract：:DNA transcription, replication, and repair are regulated by histone acetylation, a process that requires the generation of acetyl-coenzyme A (CoA). Here, we show that all the subunits of the mitochondrial pyruvate dehydrogenase complex (PDC) are also present and functional in the nucleus of mammalian cells. We found t...

journal_title：Cell

authors： Sutendra G,Kinnaird A,Dromparis P,Paulin R,Stenson TH,Haromy A,Hashimoto K,Zhang N,Flaim E,Michelakis ED

更新日期：2014-07-03 00:00:00

abstract：:The rate-limiting enzyme of cholesterol biosynthesis, HMG CoA reductase, is controlled by negative feedback regulation of transcription. We have isolated the reductase gene from a bacteriophage lambda genomic library prepared from hamster UT-1 cells. The 25 kilobase gene is split into 20 exons. The 5' untranslated and...

journal_title：Cell

authors： Reynolds GA,Basu SK,Osborne TF,Chin DJ,Gil G,Brown MS,Goldstein JL,Luskey KL

更新日期：1984-08-01 00:00:00

abstract：:Three-dimensional structures of a native simian and reassortant rotavirus have been determined by electron cryomicroscopy and computer image processing. The structural features of the native virus confirm that the hemagglutinin spike is a dimer of VP4, substantiated by in vivo radiolabeling studies. Exchange of native...

journal_title：Cell

authors： Shaw AL,Rothnagel R,Chen D,Ramig RF,Chiu W,Prasad BV

更新日期：1993-08-27 00:00:00

abstract：:Despite all modern advances in medicine, an effective drug treatment of obesity has not been found yet. Discovery of leptin two decades ago created hopes for treatment of obesity. However, development of leptin resistance has been a big obstacle, mitigating a leptin-centric treatment of obesity. Here, by using in sili...

journal_title：Cell

authors： Liu J,Lee J,Salazar Hernandez MA,Mazitschek R,Ozcan U

更新日期：2015-05-21 00:00:00

abstract：:SOCS1 is an SH2-containing protein that is primarily expressed in thymocytes in a cytokine- and T cell receptor-independent manner. SOCS1 deletion causes perinatal lethality with death by 2-3 weeks. During this period thymic changes include a loss of cellularity and a switch from predominantly CD4+ CD8+ to single posi...

journal_title：Cell

authors： Marine JC,Topham DJ,McKay C,Wang D,Parganas E,Stravopodis D,Yoshimura A,Ihle JN

更新日期：1999-09-03 00:00:00

abstract：:The heterogeneity of small extracellular vesicles and presence of non-vesicular extracellular matter have led to debate about contents and functional properties of exosomes. Here, we employ high-resolution density gradient fractionation and direct immunoaffinity capture to precisely characterize the RNA, DNA, and prot...

journal_title：Cell

authors： Jeppesen DK,Fenix AM,Franklin JL,Higginbotham JN,Zhang Q,Zimmerman LJ,Liebler DC,Ping J,Liu Q,Evans R,Fissell WH,Patton JG,Rome LH,Burnette DT,Coffey RJ

更新日期：2019-04-04 00:00:00

abstract：:The HIV auxiliary protein Vpr potently blocks the cell cycle at the G2/M transition. Here, we show that G2/M arrest results from untimely activation of the structure-specific endonuclease (SSE) regulator SLX4 complex (SLX4com) by Vpr, a process that requires VPRBP-DDB1-CUL4 E3-ligase complex. Direct interaction of Vpr...

journal_title：Cell

authors： Laguette N,Brégnard C,Hue P,Basbous J,Yatim A,Larroque M,Kirchhoff F,Constantinou A,Sobhian B,Benkirane M

更新日期：2014-01-16 00:00:00

abstract：:Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140trk-A and p75NGFR molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused...

journal_title：Cell

authors： Torcia M,Bracci-Laudiero L,Lucibello M,Nencioni L,Labardi D,Rubartelli A,Cozzolino F,Aloe L,Garaci E

更新日期：1996-05-03 00:00:00

abstract：:Suppressor of Hairless [Su(H)]/Lag-1/RBP-Jkappa/CBF1 is the only known transducing transcription factor for Notch receptor signaling. Here, we show that Su(H) has three distinct functions in the development of external mechanosensory organs in Drosophila: Notch-dependent transcriptional activation and a novel auto-rep...

journal_title：Cell

authors： Barolo S,Walker RG,Polyanovsky AD,Freschi G,Keil T,Posakony JW

更新日期：2000-12-08 00:00:00

abstract：:We describe automated technologies to probe the structure of neural tissue at nanometer resolution and use them to generate a saturated reconstruction of a sub-volume of mouse neocortex in which all cellular objects (axons, dendrites, and glia) and many sub-cellular components (synapses, synaptic vesicles, spines, spi...

journal_title：Cell

authors： Kasthuri N,Hayworth KJ,Berger DR,Schalek RL,Conchello JA,Knowles-Barley S,Lee D,Vázquez-Reina A,Kaynig V,Jones TR,Roberts M,Morgan JL,Tapia JC,Seung HS,Roncal WG,Vogelstein JT,Burns R,Sussman DL,Priebe CE,Pfister H

更新日期：2015-07-30 00:00:00

abstract：:As visualized by electron microscopy, RecA protein binds in a highly cooperative manner to single-stranded fd DNA in solutions of 0.01 M Tris (pH 7.5). The resulting nucleoprotein filament loops are 1.25 micrometers in length, have a fiber diameter of 12 nm and show an indication of a 4.5 nm repeat along the axis of t...

journal_title：Cell

authors： Dunn K,Chrysogelos S,Griffith J

更新日期：1982-04-01 00:00:00

abstract：:In this issue of Cell, Shin et al. report the first live-cell imaging of a fusion pore. Directly visualized pores in neuroendocrine cells can be much larger than expected yet not require vesicular full-collapse. These fusion-fission pores have diverse fates arising from opposing dynamin-driven pore constriction and F-...

journal_title：Cell

authors： Eyring KW,Tsien RW

更新日期：2018-05-03 00:00:00

abstract：:NRK fibroblasts exposed to transforming growth factor-beta (TGF-beta) show increased binding of radiolabeled epidermal growth factor (EGF) relative to untreated cells. The binding of another growth factor, rat insulin-like growth factor-II, is unaffected. The increase in EGF binding induced by TGF-beta is not due to i...

journal_title：Cell

authors： Assoian RK,Frolik CA,Roberts AB,Miller DM,Sporn MB

更新日期：1984-01-01 00:00:00