Abstract：
:Muscle cell cultures from Drosophila melanogaster were obtained by plating dissociated gastrula stage embryo cells on protamine-treated culture dishes. They myogenic cells in these cultures fuse to form multinucleated pulsating cells by 15 hr after plating. An analysis of protein synthesis during myogenesis in these cultures, as measured by the incorporation of 35S-methionine and analyzed by two-dimensional polyacrylamide gel electrophoresis, showed profound changes in the pattern of protein synthesis. This analysis enabled us to identify three distinct classes of proteins. Class A proteins, the most abundant, are synthesized continuously throughout myogenesis, class B proteins are those proteins whose synthesis is initiated during myogenesis and continued throughout development; class C proteins are those synthesized at specific times during development. In addition, three forms of actin have been identified in these cultures. Actin I, which shows increased synthesis concomitant with the myogenic development in these cultures, is apparently a muscle-specific form of actin. Actin II, the predominant "cytoplasmic" form of actin in the nonmuscle Schneider cell line 2, is also the major form in the gastrula cultures before differentiation begins. Synthesis of this actin continues in the myogenic cultures. Actin III is a rapidly turning over form of actin which does not accumulate in either the Schneider cells or the myogenic cultures.
journal_name
Celljournal_title
Cellauthors
Storti RV,Horovitch SJ,Scott MP,Rich A,Pardue MLdoi
10.1016/0092-8674(78)90210-6subject
Has Abstractpub_date
1978-04-01 00:00:00pages
589-98issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(78)90210-6journal_volume
13pub_type
杂志文章相关文献
CELL文献大全abstract：:Fluorescent proteins are commonly used to label cells across organisms, but the unmodified forms cannot control biological activities. Using GFP-binding proteins derived from Camelid antibodies, we co-opted GFP as a scaffold for inducing formation of biologically active complexes, developing a library of hybrid transc...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.07.021
更新日期：2013-08-15 00:00:00
abstract：:Much of the Y chromosome consists of large palindromic arrays harboring genes that are critical for spermatogenesis. In this issue, Lange et al. (2009) show that although gene conversion within these arrays maintains their integrity, it also permits rare unequal sister chromatid-exchange events within palindromes that...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2009.08.022
更新日期：2009-09-04 00:00:00
abstract：:In this issue of Cell, a study by Valerie Mizrahi and her colleagues suggests that a putative error-prone DNA polymerase encoded by the dnaE2 gene of Mycobacterium tuberculosis may bypass certain types of DNA base damage, generating mutations. This may be an important mechanism for generating drug-resistant strains of...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s0092-8674(03)00275-7
更新日期：2003-04-18 00:00:00
abstract：:The monoclonal antibody ECCD-1 inhibits Ca2+-dependent cell-cell adhesion in teratocarcinoma cells, recognizing a cell surface component of MW 124,000. When mouse embryos at various preimplantation stages were cultured in the presence of ECCD-1, the compacted morphology of the 8- to 16-cell-stage embryos was destroyed...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90095-8
更新日期：1983-12-01 00:00:00
abstract：:The adenovirus E1a region encodes two closely related gene products: 243 and 289 amino acid phosphoproteins. These proteins differ in their primary sequence only by 46 amino acids unique to the 289 amino acid protein. By constructing single-base substitution mutants we localized two functional regions of these E1a pro...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90704-x
更新日期：1986-09-26 00:00:00
abstract：:Maintenance of a constant cell volume in response to extracellular or intracellular osmotic changes is critical for cellular homeostasis. Activation of a ubiquitous volume-regulated anion channel (VRAC) plays a key role in this process; however, its molecular identity in vertebrates remains unknown. Here, we used a ce...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.03.024
更新日期：2014-04-10 00:00:00
abstract：:Several recent papers show that differences in histone modification and the use of histone variants at the 5' and 3' ends of genes influence the location and kinetics of transcriptional initiation. The ultimate target of most epigenetic mechanisms may be the regulation of nucleosome occupancy, which in turn controls a...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2005.12.010
更新日期：2005-12-29 00:00:00
abstract：:We have fused DNA fragments derived from an Escherichia coli tRNALeu1 operon to the galK gene of E. coli to identify sequences necessary for the in vivo initiation of transcription and growth-rate-dependent regulation. Promoter sequences consisting of residues from -50 to +56 or -50 to +5 with respect to the in vivo s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(82)90290-2
更新日期：1982-10-01 00:00:00
abstract：:Telomerase is the ribonucleoprotein enzyme that replenishes telomeric DNA and maintains genome integrity. Minimally, telomerase activity requires a templating RNA and a catalytic protein. Additional proteins are required for activity on telomeres in vivo. Here, we report that the Pop1, Pop6, and Pop7 proteins, known c...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.04.018
更新日期：2016-05-19 00:00:00
abstract：:Human hepatitis B virus core protein expressed in E. coli assembles into two sizes of particle. We have determined their three-dimensional structures by electron cryomicroscopy and image processing. The large and small particles correspond to triangulation number T = 4 and T = 3 dimer clustered packings, containing 24...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90142-2
更新日期：1994-06-17 00:00:00
abstract：:We have analyzed the structure of the Shrunken (Sh) locus in a strain containing an unstable recessive mutation, sh-m5933, caused by the transposable controlling element Dissociation (Ds). We have also analyzed nine spontaneous Sh revertant alleles. The sh-m5933 allele contains a 30 kb insertion at the Sh locus, as we...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90372-0
更新日期：1983-09-01 00:00:00
abstract：:Several metabolic alterations accumulate over time along with a reduction in biological fitness, suggesting the existence of a "metabolic clock" that controls aging. Multiple inborn defects in metabolic circuitries accelerate aging, whereas genetic loci linked to exceptional longevity influence metabolism. Each of the...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2016.07.031
更新日期：2016-08-11 00:00:00
abstract：:We have previously cloned lethal(2)giant larvae, a tumor-suppressor gene of Drosophila that normally controls cell proliferation and/or differentiation in the optic centers of the brain and the imaginal discs. Here we describe the structure of the l(2)gl genes as determined by sequencing genomic and cDNA clones. The s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90217-0
更新日期：1987-07-17 00:00:00
abstract：:We recently described the purification and cloning of extracellular signal-regulated kinase 1 (ERK1), which appears to play a pivotal role in converting tyrosine phosphorylation into the serine/threonine phosphorylations that regulate downstream events. We now describe cloning and characterization of two ERK1-related ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90098-j
更新日期：1991-05-17 00:00:00
abstract：:The coactivator PGC-1alpha is a key regulator of mitochondrial biogenesis and respiration, mediating expression of several transcription factors required for these programs. Three new studies reveal that PGC-1alpha expression is downregulated in patients with Huntington's disease (HD) and in several animal models of t...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.cell.2006.10.023
更新日期：2006-11-03 00:00:00
abstract：:Polycomb group proteins control a hierarchy of gene expression during the differentiation of stem cells. In this issue, Ezhkova et al. (2009) show that repression mediated by Polycomb proteins controls the timing of differentiation of precursor cells of the epidermal lineage. ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2009.03.004
更新日期：2009-03-20 00:00:00
abstract：:Salmonella possesses several flagella, each capable of counterclockwise and clockwise rotation. Counterclockwise rotation produces swimming, clockwise rotation produces tumbling. Switching between senses occurs stochastically. The rotational sense of individual flagella on a single cell could be monitored under specia...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90501-9
更新日期：1983-01-01 00:00:00
abstract：:Prolonged behavioral challenges can cause animals to switch from active to passive coping strategies to manage effort-expenditure during stress; such normally adaptive behavioral state transitions can become maladaptive in psychiatric disorders such as depression. The underlying neuronal dynamics and brainwide interac...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.02.037
更新日期：2019-05-02 00:00:00
abstract：:Nuclear pore complexes (NPCs) mediate transport across the nuclear envelope. In yeast, they also interact with active genes, attracting or retaining them at the nuclear periphery. In higher eukaryotes, some NPC components (nucleoporins) are also found in the nucleoplasm, with a so far unknown function. We have functio...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.01.011
更新日期：2010-02-05 00:00:00
abstract：:We have isolated cDNA clones encoding the four different forms of mouse myelin basic protein (MBP) and have analyzed the structure of the MBP gene. The three larger forms of MBP differ from the smallest by the inclusion of either or both of two short amino acid sequences at positions 57 and 124 of the smallest protein...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90245-4
更新日期：1985-12-01 00:00:00
abstract：:We show that expression of the iso-1-cytochrome c gene of Saccharomyces cerevisiae, CYC1, is tightly regulated by levels of intracellular heme. Expression is reduced at least 200-fold in cells grown under conditions of heme deficiency. Studies on the regulation of a CYC1-lacZ fused gene and direct determination of mRN...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90309-4
更新日期：1983-04-01 00:00:00
abstract：:This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of request of the editors. Cell is retracting this paper reporting structures of a poxvirus protein, VCP, that inhi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(01)00214-8
更新日期：2001-01-26 00:00:00
abstract：:The molybdenum-containing enzyme sulfite oxidase catalyzes the conversion of sulfite to sulfate, the terminal step in the oxidative degradation of cysteine and methionine. Deficiency of this enzyme in humans usually leads to major neurological abnormalities and early death. The crystal structure of chicken liver sulfi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80488-2
更新日期：1997-12-26 00:00:00
abstract：:V(D)J recombination of antigen receptor gene segments in B and T cells is mediated by the lymphoid-specific proteins RAG1 and RAG2. Now, Ji et al. (2010) demonstrate how RAG1 and RAG2 use DNA sequence specificity and modified histones within chromatin to target specific loci for V(D)J recombination at different stages...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.04.014
更新日期：2010-04-30 00:00:00
abstract：:The structure of the complex formed by the arginine-rich motif of the transcriptional antitermination protein N of phage lambda and boxB RNA was determined by heteronuclear magnetic resonance spectroscopy. A bent alpha helix in N recognizes primarily the shape and negatively charged surface of the boxB hairpin through...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81579-2
更新日期：1998-04-17 00:00:00
abstract：:Is the Café Scientifique a fashionable by-product of a comfortable age or an indicator of the changing relationship between science and society? ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2006.07.006
更新日期：2006-07-28 00:00:00
abstract：:The mouse t haplotypes show defects in spermatogenesis attributed to multiple loci on chromosome 17. We have cloned the gene for an abundant testicular germ cell protein, t complex polypeptide 1, which has a variant form in t haplotypes, TCP-1A. A cDNA clone, pB1.4, which hybridizes to a 19S mRNA that is abundant in h...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(86)90839-1
更新日期：1986-03-14 00:00:00
abstract：:Prion-like domains have emerged as important drivers of neurodegenerative disease. Now, Boulay et al. establish that the translocated prion-like domain of the oncogenic EWS-FLI1 fusion protein enables phase-separation events, which inappropriately recruit chromatin-remodeling factors to elicit the aberrant transcripti...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2017.09.010
更新日期：2017-09-21 00:00:00
abstract：:Thrombin stimulates 32Pi incorporation into phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol 4,5-bis-phosphate (PIP2), and phosphatidylinositol (PI), and initiates DNA synthesis in hamster (NIL) fibroblasts at a half-maximal concentration of 125 ng/ml. Neomycin, which binds PIP2 and PIP, inhibits both thro...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90105-9
更新日期：1985-09-01 00:00:00
abstract：:The activation of the tumor suppressor p53 facilitates the cellular response to genotoxic stress; however, the p53 response can only be executed if its interaction with its inhibitor Mdm2 is abolished. There have been conflicting reports on the question of whether p53 posttranslational modifications, such as phosphory...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.03.025
更新日期：2008-05-16 00:00:00
