Acetylation is indispensable for p53 activation.


:The activation of the tumor suppressor p53 facilitates the cellular response to genotoxic stress; however, the p53 response can only be executed if its interaction with its inhibitor Mdm2 is abolished. There have been conflicting reports on the question of whether p53 posttranslational modifications, such as phosphorylation or acetylation, are essential or only play a subtle, fine-tuning role in the p53 response. Thus, it remains unclear whether p53 modification is absolutely required for its activation. We have now identified all major acetylation sites of p53. Although unacetylated p53 retains its ability to induce the p53-Mdm2 feedback loop, loss of acetylation completely abolishes p53-dependent growth arrest and apoptosis. Notably, acetylation of p53 abrogates Mdm2-mediated repression by blocking the recruitment of Mdm2 to p53-responsive promoters, which leads to p53 activation independent of its phosphorylation status. Our study identifies p53 acetylation as an indispensable event that destabilizes the p53-Mdm2 interaction and enables the p53-mediated stress response.






Tang Y,Zhao W,Chen Y,Zhao Y,Gu W




Has Abstract


2008-05-16 00:00:00
















  • Ribosome binding to reovirus mRNA in protein synthesis requires 5' terminal 7-methylguanosine.

    abstract::Purified reovirus synthesizes in vitro a mixture of mRNA molecules that contain 5' terminal structures of the type ppG..., GpppG..., and m7GpppGm, the relative amount of each depending upon the presence of S-adenosylmethionine (SAM) or S-adenosylhomocysteine (SAH) in the transcription incubation mixture. Reovirus mRNA...


    pub_type: 杂志文章


    authors: Both GW,Furuichi Y,Muthukrishnan S,Shatkin AJ

    更新日期:1975-10-01 00:00:00

  • Cloning and characterization of the integrated viral DNA from three lines of SV40-transformed mouse cells.

    abstract::Recombinant DNA techniques have been used to isolate the integrated viral DNA, together with flanking cellular sequences, from three independently isolated lines of SV40-transformed BALB/c 3T3 cells. We have determined the structures of these cloned segments and their relationships to one another by restriction enzyme...


    pub_type: 杂志文章


    authors: Clayton CE,Rigby PW

    更新日期:1981-08-01 00:00:00

  • Probing DNA by 2-OG-dependent dioxygenase.

    abstract::TET-mediated 5-methyl cytosine (5mC) oxidation acts in epigenetic regulation, stem cell development, and cancer. Hu et al. now determine the crystal structure of the TET2 catalytic domain bound to DNA, shedding light on 5mC-DNA substrate recognition and the catalytic mechanism of 5mC oxidation. ...


    pub_type: 评论,杂志文章


    authors: Tsai CL,Tainer JA

    更新日期:2013-12-19 00:00:00

  • The origin of replication of plasmid p15A and comparative studies on the nucleotide sequences around the origin of related plasmids.

    abstract::Replication of Escherichia coli plasmid p15A was examined by use of a cell extract or a mixture of three purified E. coli enzymes: RNA polymerase; RNAase H; and DNA polymerase I. In each system, replication initiates at any of three consecutive nucleotides located at a unique site. Primer transcription starts 508 bp u...


    pub_type: 杂志文章


    authors: Selzer G,Som T,Itoh T,Tomizawa J

    更新日期:1983-01-01 00:00:00

  • A kinesin-like protein is required for germ plasm aggregation in Xenopus.

    abstract::In embryos of X. laevis, and many other species, early development requires targeted movements of molecules and molecular aggregates within the oocyte or egg cytoplasm. One well-known example in Xenopus is the aggregation of germ plasm, a group of cytoplasmic islands that become distributed during the first few cell c...


    pub_type: 杂志文章


    authors: Robb DL,Heasman J,Raats J,Wylie C

    更新日期:1996-11-29 00:00:00

  • The polycomb protein Pc2 is a SUMO E3.

    abstract::Polycomb group (PcG) proteins form large multimeric complexes (PcG bodies) which are involved in the stable repression of gene expression. The human PcG protein, Pc2, has been shown to recruit the transcriptional corepressor, CtBP, to PcG bodies. We show that CtBP is sumoylated at a single lysine. In vitro, CtBP sumoy...


    pub_type: 杂志文章


    authors: Kagey MH,Melhuish TA,Wotton D

    更新日期:2003-04-04 00:00:00

  • A human interactome in three quantitative dimensions organized by stoichiometries and abundances.

    abstract::The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have...


    pub_type: 杂志文章


    authors: Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

    更新日期:2015-10-22 00:00:00

  • An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor.

    abstract::Characterizing cell surface receptors mediating viral infection is critical for understanding viral tropism and developing antiviral therapies. Nevertheless, due to challenges associated with detecting protein interactions on the cell surface, the host receptors of many human pathogens remain unknown. Here, we build a...


    pub_type: 杂志文章


    authors: Martinez-Martin N,Marcandalli J,Huang CS,Arthur CP,Perotti M,Foglierini M,Ho H,Dosey AM,Shriver S,Payandeh J,Leitner A,Lanzavecchia A,Perez L,Ciferri C

    更新日期:2018-08-23 00:00:00

  • The genetic basis for specific anosmia to isovaleric acid in the mouse.

    abstract::The detection and discrimination of odorants in mammals is thought to be mediated by a family of 100-1000 seven transmembrane domain receptor proteins, although none of these putative olfactory receptors have been shown to bind individual odorants with high affinity. We have used a genetic approach to identify the gen...


    pub_type: 杂志文章


    authors: Griff IC,Reed RR

    更新日期:1995-11-03 00:00:00

  • Genome regulation by polycomb and trithorax proteins.

    abstract::Polycomb group (PcG) and trithorax group (trxG) proteins are critical regulators of numerous developmental genes. To silence or activate gene expression, respectively, PcG and trxG proteins bind to specific regions of DNA and direct the posttranslational modification of histones. Recent work suggests that PcG proteins...


    pub_type: 杂志文章,评审


    authors: Schuettengruber B,Chourrout D,Vervoort M,Leblanc B,Cavalli G

    更新日期:2007-02-23 00:00:00

  • Switch between large hand-over-hand and small inchworm-like steps in myosin VI.

    abstract::Many biological motor molecules move within cells using stepsizes predictable from their structures. Myosin VI, however, has much larger and more broadly distributed stepsizes than those predicted from its short lever arms. We explain the discrepancy by monitoring Qdots and gold nanoparticles attached to the myosin-VI...


    pub_type: 杂志文章


    authors: Nishikawa S,Arimoto I,Ikezaki K,Sugawa M,Ueno H,Komori T,Iwane AH,Yanagida T

    更新日期:2010-09-17 00:00:00

  • Six-microns-under acts upstream of Draper in the glial phagocytosis of apoptotic neurons.

    abstract::The removal of apoptotic cells by phagocytic neighbors is essential for metazoan development but remains poorly characterized. Here we report the discovery of a Drosophila phagocytosis receptor, Six-microns-under (SIMU), which is expressed in highly phagocytic cell types during development and required for efficient a...


    pub_type: 杂志文章


    authors: Kurant E,Axelrod S,Leaman D,Gaul U

    更新日期:2008-05-02 00:00:00

  • Old drugs, new tricks: using genetically sensitized yeast to reveal drug targets.

    abstract::A study in this issue of Cell illustrates the power of applying genomic approaches with model systems to characterize the biological activity of small molecules and to identify their cellular targets, which can clarify the mode of action of human therapeutics. ...


    pub_type: 评论,杂志文章,评审


    authors: Hughes T,Andrews B,Boone C

    更新日期:2004-01-09 00:00:00

  • Integrated viral sequences in adenovirus type 12-transformed hamster cells.

    abstract::The physical state of the viral genome in four lines of hamster cells transformed by adenovirus type 12 (Ad12) has been investigated. The four lines of transformed cells originated from hamster cells after infection with Ad12 at multiplicities ranging from 5-350 plaque-forming units per cell. The DNA from transformed ...


    pub_type: 杂志文章


    authors: Groneberg J,Chardonnet Y,Doerfler W

    更新日期:1977-01-01 00:00:00

  • Transcription of specific genes in isolated nuclei by exogenous RNA polymerases.

    abstract::Mouse plasmacytoma (MOPC) 460 cells contain two chromatographic forms of RNA polymerase III (IIIA and IIIB) in addition to the major class I and II RNA polymerases. Nuclei isolated from these cells actively synthesize RNA. Among the discrete transcription products observed are the 5S and 4.5S RNAs and additional low m...


    pub_type: 杂志文章


    authors: Sklar VE,Roeder RG

    更新日期:1977-03-01 00:00:00

  • Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals.

    abstract::The commitment complex is the first defined step in the yeast (S. cerevisiae) splicing pathway. It contains U1 snRNP as well as Mud2p, which resembles human U2AF65. In a genetic screen, we identified the yeast gene MSL-5, which is a novel commitment complex component. Genetic and biochemical criteria indicate a direct...


    pub_type: 杂志文章


    authors: Abovich N,Rosbash M

    更新日期:1997-05-02 00:00:00

  • The existence of an insoluble Z disc scaffold in chicken skeletal muscle.

    abstract::Extraction of glycerinated chicken skeletal muscle with 0.6 M potassium iodide leaves a framework of insoluble components within each muscle fiber. This framework is composed primarily of planes of in-register Z discs that have been thickened by the accumulation of material on both sides of each disc during extraction...


    pub_type: 杂志文章


    authors: Granger BL,Lazarides E

    更新日期:1978-12-01 00:00:00

  • Will the ubiquitin system furnish as many drug targets as protein kinases?

    abstract::Protein phosphorylation and protein ubiquitination regulate most aspects of cell life, and defects in these control mechanisms cause cancer and many other diseases. In the past decade, protein kinases have become one of the most important classes of drug targets for the pharmaceutical industry. In contrast, drug disco...


    pub_type: 杂志文章


    authors: Cohen P,Tcherpakov M

    更新日期:2010-11-24 00:00:00

  • Interaction of fibronectin with its receptor on platelets.

    abstract::We report that the 12,000 dalton domain of fibronectin that interacts with fibroblast cell surfaces also binds specifically to thrombin-inducible, saturable receptors on platelets. Furthermore, we have used chemical cross-linking and monoclonal antibodies to show that the 12,000 dalton domain of fibronectin interacts ...


    pub_type: 杂志文章


    authors: Gardner JM,Hynes RO

    更新日期:1985-09-01 00:00:00

  • Representation of the glomerular olfactory map in the Drosophila brain.

    abstract::We explored how the odor map in the Drosophila antennal lobe is represented in higher olfactory centers, the mushroom body and lateral horn. Systematic single-cell tracing of projection neurons (PNs) that send dendrites to specific glomeruli in the antennal lobe revealed their stereotypical axon branching patterns and...


    pub_type: 杂志文章


    authors: Marin EC,Jefferis GS,Komiyama T,Zhu H,Luo L

    更新日期:2002-04-19 00:00:00

  • Crosstalk between muscularis macrophages and enteric neurons regulates gastrointestinal motility.

    abstract::Intestinal peristalsis is a dynamic physiologic process influenced by dietary and microbial changes. It is tightly regulated by complex cellular interactions; however, our understanding of these controls is incomplete. A distinct population of macrophages is distributed in the intestinal muscularis externa. We demonst...


    pub_type: 杂志文章


    authors: Muller PA,Koscsó B,Rajani GM,Stevanovic K,Berres ML,Hashimoto D,Mortha A,Leboeuf M,Li XM,Mucida D,Stanley ER,Dahan S,Margolis KG,Gershon MD,Merad M,Bogunovic M

    更新日期:2014-07-17 00:00:00

  • Zip2, a meiosis-specific protein required for the initiation of chromosome synapsis.

    abstract::We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic ch...


    pub_type: 杂志文章


    authors: Chua PR,Roeder GS

    更新日期:1998-05-01 00:00:00

  • Disconnected: a locus required for neuronal pathway formation in the visual system of Drosophila.

    abstract::Mutations at the X-linked disconnected locus of D. melanogaster lead to the failure of adult photoreceptor axons to innervate their target cells in the developing optic lobes of the third instar larva, resulting in flies that have rudimentary optic ganglia. The cascade of epigenetic events leading to the adult disconn...


    pub_type: 杂志文章


    authors: Steller H,Fischbach KF,Rubin GM

    更新日期:1987-09-25 00:00:00

  • Backbone mutations in transmembrane domains of a ligand-gated ion channel: implications for the mechanism of gating.

    abstract::An approach to identify backbone conformational changes underlying nicotinic acetylcholine receptor (nAChR) gating was developed. Specific backbone peptide bonds were replaced with an ester, which disrupts backbone hydrogen bonds at the site of mutation. At a conserved proline residue (alphaPro221) in the first transm...


    pub_type: 杂志文章


    authors: England PM,Zhang Y,Dougherty DA,Lester HA

    更新日期:1999-01-08 00:00:00

  • GroEL1: a dedicated chaperone involved in mycolic acid biosynthesis during biofilm formation in mycobacteria.

    abstract::Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2. GroEL2 is essential--presumably providing the housekeeping chaperone functions--while groEL1 is nonessential, contains the attB site for phage Bxb1 integration, and encodes a putative chaperone with unusual structural features. Inactivation of...


    pub_type: 杂志文章


    authors: Ojha A,Anand M,Bhatt A,Kremer L,Jacobs WR Jr,Hatfull GF

    更新日期:2005-12-02 00:00:00

  • The P. furiosus mre11/rad50 complex promotes 5' strand resection at a DNA double-strand break.

    abstract::The Mre11/Rad50 complex has been implicated in the early steps of DNA double-strand break (DSB) repair through homologous recombination in several organisms. However, the enzymatic properties of this complex are incompatible with the generation of 3' single-stranded DNA for recombinase loading and strand exchange. In ...


    pub_type: 杂志文章


    authors: Hopkins BB,Paull TT

    更新日期:2008-10-17 00:00:00

  • Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System.

    abstract::During neural tube closure and spinal cord development, many cells die in both the central and peripheral nervous systems (CNS and PNS, respectively). However, myeloid-derived professional phagocytes have not yet colonized the trunk region during early neurogenesis. How apoptotic cells are removed from this region dur...


    pub_type: 杂志文章


    authors: Zhu Y,Crowley SC,Latimer AJ,Lewis GM,Nash R,Kucenas S

    更新日期:2019-09-19 00:00:00

  • Nuclear PTEN regulates the APC-CDH1 tumor-suppressive complex in a phosphatase-independent manner.

    abstract::PTEN is a frequently mutated tumor suppressor gene that opposes the PI3K/AKT pathway through dephosphorylation of phosphoinositide-3,4,5-triphosphate. Recently, nuclear compartmentalization of PTEN was found as a key component of its tumor-suppressive activity; however its nuclear function remains poorly defined. Here...


    pub_type: 杂志文章


    authors: Song MS,Carracedo A,Salmena L,Song SJ,Egia A,Malumbres M,Pandolfi PP

    更新日期:2011-01-21 00:00:00

  • Solution structure of a specific DNA complex of the Myb DNA-binding domain with cooperative recognition helices.

    abstract::The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be...


    pub_type: 杂志文章


    authors: Ogata K,Morikawa S,Nakamura H,Sekikawa A,Inoue T,Kanai H,Sarai A,Ishii S,Nishimura Y

    更新日期:1994-11-18 00:00:00

  • Critical role of amyloid-like oligomers of Drosophila Orb2 in the persistence of memory.

    abstract::A long-standing question in the study of long-term memory is how a memory trace persists for years when the proteins that initiated the process turn over and disappear within days. Previously, we postulated that self-sustaining amyloidogenic oligomers of cytoplasmic polyadenylation element-binding protein (CPEB) provi...


    pub_type: 杂志文章


    authors: Majumdar A,Cesario WC,White-Grindley E,Jiang H,Ren F,Khan MR,Li L,Choi EM,Kannan K,Guo F,Unruh J,Slaughter B,Si K

    更新日期:2012-02-03 00:00:00