当前位置： SCI文献检索 > CELL期刊下所有文献 > Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA.

Methylation of deoxycytidine incorporated by excision-repair synthesis of DNA.

Abstract：

:Methylation of deoxycytidine incorporated by DNA excision-repair was studied in human diploid fibroblasts following damage with ultraviolet radiation, N-methyl-N-nitrosourea, or N-acetoxy-2-acetylaminofluorene. In confluent, nondividing cells, methylation in repair patches induced by all three agents is slow and incomplete. Whereas after DNA replication in logarithmic-phase cultures a steady state level of 3.4% 5-methylcytosine is reached in less than 2 hr after cells are labeled with 6- 3H-deoxycytidine, following ultraviolet-stimulated repair synthesis in confluent cells it takes about 3 days to reach a level of approximately 2.0% 5-methylcytosine in the repair patch. In cells from cultures in logarithmic-phase growth, 5-methylcytosine formation in ultraviolet-induced repair patches occurs faster and to a greater extent, reaching a level of approximately 2.7% in 10-20 hr. Preexisting hypomethylated repair patches in confluent cells are methylated further when the cells are stimulated to divide; however, the repair patch may still not be fully methylated before cell division occurs. Thus DNA damage and repair may lead to heritable loss of methylation at some sites.

journal_name

Cell

journal_title

Cell

authors

Kastan MB,Gowans BJ,Lieberman MW

doi

10.1016/0092-8674(82)90248-3

subject

Has Abstract

pub_date

1982-09-01 00:00:00

pages

509-16

issue

2

eissn

0092-8674

issn

1097-4172

pii

0092-8674(82)90248-3

journal_volume

30

pub_type

杂志文章

相关文献

CELL文献大全
  • A dual-tropic primary HIV-1 isolate that uses fusin and the beta-chemokine receptors CKR-5, CKR-3, and CKR-2b as fusion cofactors.

    abstract：:Here, we show that the beta-chemokine receptor CKR-5 serves as a cofactor for M-tropic HIV viruses. Expression of CKR-5 with CD4 enables nonpermissive cells to form syncytia with cells expressing M-tropic, but not T-tropic, HIV-1 env proteins. Expression of CKR-5 and CD4 enables entry of a M-tropic, but not a T-tropic...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)81314-8

    authors： Doranz BJ,Rucker J,Yi Y,Smyth RJ,Samson M,Peiper SC,Parmentier M,Collman RG,Doms RW

    更新日期：1996-06-28 00:00:00

  • A mechanism of formation of a persistent hybrid between elongating RNA and template DNA.

    abstract：:At the replication origin of ColE1 plasmid, a persistent hybrid is formed between the primer precursor (RNA II) and its template DNA. The wild-type sequence in the region 13 to 20 bp upstream (-20 region) of the origin is required to form this persistent hybrid. While the template strand for transcription of this regi...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(90)90370-t

    authors： Masukata H,Tomizawa J

    更新日期：1990-07-27 00:00:00

  • Trimerization of a yeast transcriptional activator via a coiled-coil motif.

    abstract：:The transcriptional induction of heat shock genes is mediated by the heat shock transcription factor (HSF). We have investigated the oligomerization state of HSF from S. cerevisiae and find that it forms a trimer in solution and when bound to DNA. Trimerization is mediated by a region of HSF that, like the leucine zip...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(89)90604-1

    authors： Sorger PK,Nelson HC

    更新日期：1989-12-01 00:00:00

  • Folding and quality control of the VHL tumor suppressor proceed through distinct chaperone pathways.

    abstract：:The mechanisms by which molecular chaperones assist quality control of cytosolic proteins are poorly understood. Analysis of the chaperone requirements for degradation of misfolded variants of a cytosolic protein, the VHL tumor suppressor, reveals that distinct chaperone pathways mediate its folding and quality contro...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2005.03.024

    authors： McClellan AJ,Scott MD,Frydman J

    更新日期：2005-06-03 00:00:00

  • DBP, a liver-enriched transcriptional activator, is expressed late in ontogeny and its tissue specificity is determined posttranscriptionally.

    abstract：:The full-length cDNA for a transcriptional activator, DBP, that binds to the D site of the albumin promoter has been cloned. DBP belongs to a family of related transcription factors including Fos, Jun, CREB, and C/EBP, which share a conserved basic domain. However, unlike most other members of this family, DBP does no...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(90)90808-r

    authors： Mueller CR,Maire P,Schibler U

    更新日期：1990-04-20 00:00:00

  • The DHR78 nuclear receptor is required for ecdysteroid signaling during the onset of Drosophila metamorphosis.

    abstract：:Pulses of ecdysteroids direct Drosophila through its life cycle by activating stage- and tissue-specific genetic regulatory hierarchies. Here we show that an orphan nuclear receptor, DHR78, functions at the top of the ecdysteroid regulatory hierarchies. Null mutations in DHR78 lead to lethality during the third larval...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)81184-8

    authors： Fisk GJ,Thummel CS

    更新日期：1998-05-15 00:00:00

  • Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion.

    abstract：:We describe genetic and physical characterization of rearrangements of chromosome III which result in changes of cell type in S. cerevisiae. Two types of rearrangements were obtained as rare events which caused a change at the locus controlling cell type, MAT, associated with a recessive lethal mutation, in one case f...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(79)90050-3

    authors： Strathern JN,Newlon CS,Herskowitz I,Hicks JB

    更新日期：1979-10-01 00:00:00

  • A Deep Learning Approach to Antibiotic Discovery.

    abstract：:Due to the rapid emergence of antibiotic-resistant bacteria, there is a growing need to discover new antibiotics. To address this challenge, we trained a deep neural network capable of predicting molecules with antibacterial activity. We performed predictions on multiple chemical libraries and discovered a molecule fr...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2020.01.021

    authors： Stokes JM,Yang K,Swanson K,Jin W,Cubillos-Ruiz A,Donghia NM,MacNair CR,French S,Carfrae LA,Bloom-Ackermann Z,Tran VM,Chiappino-Pepe A,Badran AH,Andrews IW,Chory EJ,Church GM,Brown ED,Jaakkola TS,Barzilay R,Collins J

    更新日期：2020-02-20 00:00:00

  • In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence.

    abstract：:A gene for Saccharomyces cerevisiae tRNATrp has been sequenced which contains an intervening sequence of 34 bp (H. S. Kang and J. Abelson, unpublished results). The mutant yeast strain ts-136 accumulates a precursor to tRNATrp which contains mature ends and is colinear with the tRNATrp gene. A nuclear extract from Xen...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(79)90166-1

    authors： Ogden RC,Beckman JS,Abelson J,Kang HS,Söll D,Schmidt O

    更新日期：1979-06-01 00:00:00

  • CRISPR-Cas9 knockin mice for genome editing and cancer modeling.

    abstract：:CRISPR-Cas9 is a versatile genome editing technology for studying the functions of genetic elements. To broadly enable the application of Cas9 in vivo, we established a Cre-dependent Cas9 knockin mouse. We demonstrated in vivo as well as ex vivo genome editing using adeno-associated virus (AAV)-, lentivirus-, or parti...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2014.09.014

    authors： Platt RJ,Chen S,Zhou Y,Yim MJ,Swiech L,Kempton HR,Dahlman JE,Parnas O,Eisenhaure TM,Jovanovic M,Graham DB,Jhunjhunwala S,Heidenreich M,Xavier RJ,Langer R,Anderson DG,Hacohen N,Regev A,Feng G,Sharp PA,Zhang F

    更新日期：2014-10-09 00:00:00

  • Maternally transmitted resistance to lymphoma development in mice of reciprocal crosses of the RF/J and AKR/J strains.

    abstract：:Females but not males of the low-lymphoma RF/J strain transmit a non-Mendelian factor which suppresses the development of lymphoma in F1 crosses with mice of the high-lymphoma AKR/J strain. Suppression of lymphoma was also evident in the first backcross generation to the parental AKR strain, but only when (RF female x...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(80)90517-6

    authors： Mayer A,Struuck FD,Duran-Reynals ML,Lilly F

    更新日期：1980-02-01 00:00:00

  • Germ-cell loss extends C. elegans life span through regulation of DAF-16 by kri-1 and lipophilic-hormone signaling.

    abstract：:In C. elegans, removing the germ cells extends life span by triggering the nuclear localization and activation of the DAF-16/FOXO transcription factor in the intestine. In this study, we identify and analyze genes required for germline removal to extend life span. We find that the reproductive system communicates with...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2006.01.039

    authors： Berman JR,Kenyon C

    更新日期：2006-03-10 00:00:00

  • Vesicular stomatitis virus glycoprotein is sorted and concentrated during export from the endoplasmic reticulum.

    abstract：:Newly synthesized proteins are believed to move from the endoplasmic reticulum (ER) to the Golgi by bulk flow, and sorting is assumed to occur exclusively in the trans-Golgi network (TGN). Using quantitative immunoelectron microscopy, we demonstrate that vesicular stomatitis virus glycoprotein (VSV-G) is sorted from r...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(94)90359-x

    authors： Balch WE,McCaffery JM,Plutner H,Farquhar MG

    更新日期：1994-03-11 00:00:00

  • Self-eating limits EGFR-dependent tumor growth.

    abstract：:Autophagy is a cell-autonomous, catabolic process that plays context-dependent roles in tumor growth and progression. Wei et al. report that EGFR signaling promotes tumor growth through phosphorylation and functional inactivation of Beclin 1 and the consequent suppression of autophagy. ...

    journal_title：Cell

    pub_type： 评论,杂志文章

    doi：10.1016/j.cell.2013.08.040

    authors： Fantin VR,Abraham RT

    更新日期：2013-09-12 00:00:00

  • An altered pattern of cross-resistance in multidrug-resistant human cells results from spontaneous mutations in the mdr1 (P-glycoprotein) gene.

    abstract：:Multidrug resistance in human cells results from increased expression of the mdr1 (P-glycoprotein) gene. Although the same gene is activated in cells selected with different drugs, multidrug-resistant cell lines can be preferentially resistant to their selecting agent. The mdr1 cDNA sequence from vinblastine-selected ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(88)90568-5

    authors： Choi KH,Chen CJ,Kriegler M,Roninson IB

    更新日期：1988-05-20 00:00:00

  • Fetal anemia and apoptosis of red cell progenitors in Stat5a-/-5b-/- mice: a direct role for Stat5 in Bcl-X(L) induction.

    abstract：:The erythropoietin receptor (EpoR) is essential for production of red blood cells; a principal function of EpoR is to rescue committed erythroid progenitors from apoptosis. Stat5 is rapidly activated following EpoR stimulation, but its function in erythropoiesis has been unclear since adult Stat5a-/-5b-/- mice have no...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)81013-2

    authors： Socolovsky M,Fallon AE,Wang S,Brugnara C,Lodish HF

    更新日期：1999-07-23 00:00:00

  • Mechanism of replication-coupled DNA interstrand crosslink repair.

    abstract：:DNA interstrand crosslinks (ICLs) are toxic DNA lesions whose repair occurs in the S phase of metazoans via an unknown mechanism. Here, we describe a cell-free system based on Xenopus egg extracts that supports ICL repair. During DNA replication of a plasmid containing a site-specific ICL, two replication forks conver...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2008.08.030

    authors： Räschle M,Knipscheer P,Enoiu M,Angelov T,Sun J,Griffith JD,Ellenberger TE,Schärer OD,Walter JC

    更新日期：2008-09-19 00:00:00

  • A role for hematopoietic stem cells in promoting angiogenesis.

    abstract：:Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embry...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)00025-8

    authors： Takakura N,Watanabe T,Suenobu S,Yamada Y,Noda T,Ito Y,Satake M,Suda T

    更新日期：2000-07-21 00:00:00

  • The Mitochondrial Unfolded Protein Response Is Mediated Cell-Non-autonomously by Retromer-Dependent Wnt Signaling.

    abstract：:The mitochondrial unfolded protein response (UPRmt) can be triggered in a cell-non-autonomous fashion across multiple tissues in response to mitochondrial dysfunction. The ability to communicate information about the presence of mitochondrial stress enables a global response that can ultimately better protect an organ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2018.06.029

    authors： Zhang Q,Wu X,Chen P,Liu L,Xin N,Tian Y,Dillin A

    更新日期：2018-08-09 00:00:00

  • Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitro.

    abstract：:We present evidence that the genes encoding U3 snRNA in plants are transcribed by RNA polymerase III (pol III) and not by RNA polymerase II (pol II) as in vertebrates or lower eukaryotes. The U3 gene is the only known example of a gene transcribed by different polymerases in different organisms. It is possible to conv...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(91)90469-f

    authors： Kiss T,Marshallsay C,Filipowicz W

    更新日期：1991-05-03 00:00:00

  • Membrane hemifusion: crossing a chasm in two leaps.

    abstract：:During membrane fusion, the outer leaflets of the two membranes merge first, whereas the distal membrane leaflets remain separate until the opening of a fusion pore. This intermediate stage, called hemifusion, is a critical event shared by exocytosis, protein trafficking, and viral entry. ...

    journal_title：Cell

    pub_type： 杂志文章,评审

    doi：10.1016/j.cell.2005.10.015

    authors： Chernomordik LV,Kozlov MM

    更新日期：2005-11-04 00:00:00

  • CHL1 functions as a nitrate sensor in plants.

    abstract：:Ions serve as essential nutrients in higher plants and can also act as signaling molecules. Little is known about how plants sense changes in soil nutrient concentrations. Previous studies showed that T101-phosphorylated CHL1 is a high-affinity nitrate transporter, whereas T101-dephosphorylated CHL1 is a low-affinity ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2009.07.004

    authors： Ho CH,Lin SH,Hu HC,Tsay YF

    更新日期：2009-09-18 00:00:00

  • Viral RNAs: lessons from the enemy.

    abstract：:Viruses are adept at evolving or co-opting genomic elements that allow them to maximize their replication potential in the infected host. This evolutionary plasticity makes viruses an invaluable system to identify new mechanisms used not only by viruses but also by vertebrate cells to modulate gene expression. Here, I...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2009.01.048

    authors： Cullen BR

    更新日期：2009-02-20 00:00:00

  • Single-Cell Transcriptomics Reveals Early Emergence of Liver Parenchymal and Non-parenchymal Cell Lineages.

    abstract：:The cellular complexity and scale of the early liver have constrained analyses examining its emergence during organogenesis. To circumvent these issues, we analyzed 45,334 single-cell transcriptomes from embryonic day (E)7.5, when endoderm progenitors are specified, to E10.5 liver, when liver parenchymal and non-paren...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2020.09.012

    authors： Lotto J,Drissler S,Cullum R,Wei W,Setty M,Bell EM,Boutet SC,Nowotschin S,Kuo YY,Garg V,Pe'er D,Church DM,Hadjantonakis AK,Hoodless PA

    更新日期：2020-10-29 00:00:00

  • Adaptation in bacterial chemotaxis: CheB-dependent modification permits additional methylations of sensory transducer proteins.

    abstract：:Sensory transduction in E. coli consists of two phases, excitation and adaptation, both of which involve the methyl-accepting chemotaxis proteins (MCPs). These molecules relay transmembrane signals and are reversibly methylated during adaptation of E. coli to environmental stimuli. Each MCP contains multiple sites of ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(82)90438-x

    authors： Kehry MR,Dahlquist FW

    更新日期：1982-07-01 00:00:00

  • Evidence for a common evolutionary origin of inverted repeat transposons in Drosophila and plants: hobo, Activator, and Tam3.

    abstract：:We have sequenced HFL1 from D. melanogaster, the only cloned hobo element shown to have transposase activity. The 2959 bp HFL1 sequence predicts a 2.0 kb open reading frame (ORF1) with substantial amino acid similarity to the transposases of Activator (Ac) from maize (Zea mays) and Tam3 from snapdragon (Antirrhinum ma...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(81)90010-6

    authors： Calvi BR,Hong TJ,Findley SD,Gelbart WM

    更新日期：1991-08-09 00:00:00

  • Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses.

    abstract：:To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2017.04.014

    authors： Lavin Y,Kobayashi S,Leader A,Amir ED,Elefant N,Bigenwald C,Remark R,Sweeney R,Becker CD,Levine JH,Meinhof K,Chow A,Kim-Shulze S,Wolf A,Medaglia C,Li H,Rytlewski JA,Emerson RO,Solovyov A,Greenbaum BD,Sanders C,Vi

    更新日期：2017-05-04 00:00:00

  • MIX-1: an essential component of the C. elegans mitotic machinery executes X chromosome dosage compensation.

    abstract：:We show that a functional component of the C. elegans mitotic machinery regulates X chromosome gene expression. This protein, MIX-1, is a member of the dosage compensation complex that associates specifically with hermaphrodite X chromosomes to reduce their gene expression during interphase. MIX-1 also associates with...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/s0092-8674(00)80920-4

    authors： Lieb JD,Albrecht MR,Chuang PT,Meyer BJ

    更新日期：1998-01-23 00:00:00

  • DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.

    abstract：:DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins. dmc1 mutants are defective in reciprocal recombination, accumulate double-strand break (DSB) recombination intermediates, fail to form normal synaptonemal complex (SC), and arrest late in meiotic prophase. dmc1 ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/0092-8674(92)90446-j

    authors： Bishop DK,Park D,Xu L,Kleckner N

    更新日期：1992-05-01 00:00:00

  • Structure-guided reprogramming of human cGAS dinucleotide linkage specificity.

    abstract：:Cyclic dinucleotides (CDNs) play central roles in bacterial pathogenesis and innate immunity. The mammalian enzyme cGAS synthesizes a unique cyclic dinucleotide (cGAMP) containing a 2'-5' phosphodiester linkage essential for optimal immune stimulation, but the molecular basis for linkage specificity is unknown. Here, ...

    journal_title：Cell

    pub_type： 杂志文章

    doi：10.1016/j.cell.2014.07.028

    authors： Kranzusch PJ,Lee ASY,Wilson SC,Solovykh MS,Vance RE,Berger JM,Doudna JA

    更新日期：2014-08-28 00:00:00