CHL1 functions as a nitrate sensor in plants.

Abstract:

:Ions serve as essential nutrients in higher plants and can also act as signaling molecules. Little is known about how plants sense changes in soil nutrient concentrations. Previous studies showed that T101-phosphorylated CHL1 is a high-affinity nitrate transporter, whereas T101-dephosphorylated CHL1 is a low-affinity transporter. In this study, analysis of an uptake- and sensing-decoupled mutant showed that the nitrate transporter CHL1 functions as a nitrate sensor. Primary nitrate responses in CHL1T101D and CHLT101A transgenic plants showed that phosphorylated and dephosphorylated CHL1 lead to a low- and high-level response, respectively. In vitro and in vivo studies showed that, in response to low nitrate concentrations, protein kinase CIPK23 can phosphorylate T101 of CHL1 to maintain a low-level primary response. Thus, CHL1 uses dual-affinity binding and a phosphorylation switch to sense a wide range of nitrate concentrations in the soil, thereby functioning as an ion sensor in higher plants. For a video summary of this article, see the PaperFlick file with the Supplemental Data available online.

journal_name

Cell

journal_title

Cell

authors

Ho CH,Lin SH,Hu HC,Tsay YF

doi

10.1016/j.cell.2009.07.004

subject

Has Abstract

pub_date

2009-09-18 00:00:00

pages

1184-94

issue

6

eissn

0092-8674

issn

1097-4172

pii

S0092-8674(09)00842-3

journal_volume

138

pub_type

杂志文章

相关文献

CELL文献大全
  • Mechanism by which Liddle's syndrome mutations increase activity of a human epithelial Na+ channel.

    abstract::Liddle's syndrome is an inherited form of hypertension caused by mutations that truncate the C-terminus of human epithelial Na+ channel (hENaC) subunits. Expression of truncated beta and gamma hENaC subunits increased Na+ current. However, truncation did not alter single-channel conductance or open state probability, ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(95)90212-0

    authors: Snyder PM,Price MP,McDonald FJ,Adams CM,Volk KA,Zeiher BG,Stokes JB,Welsh MJ

    更新日期:1995-12-15 00:00:00

  • HIV nuclear import is governed by the phosphotyrosine-mediated binding of matrix to the core domain of integrase.

    abstract::The karyophilic properties of the viral matrix (MA) protein govern HIV nuclear import in nondividing cells such as macrophages. A critical regulator of this process is the C-terminal tyrosine phosphorylation of MA during virus maturation. Here, we reveal the mechanism of this phenomenon, by demonstrating that tyrosine...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(95)90097-7

    authors: Gallay P,Swingler S,Song J,Bushman F,Trono D

    更新日期:1995-11-17 00:00:00

  • A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B.

    abstract::Cyclin B is degraded at the onset of anaphase by a ubiquitin-dependent proteolytic system. We have fractionated mitotic Xenopus egg extracts to identify components required for this process. We find that UBC4 and at least one other ubiquitin-conjugating enzyme can support cyclin B ubiquitination. The mitotic specifici...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(95)90338-0

    authors: King RW,Peters JM,Tugendreich S,Rolfe M,Hieter P,Kirschner MW

    更新日期:1995-04-21 00:00:00

  • A role for exon sequences and splice-site proximity in splice-site selection.

    abstract::Analysis of the in vitro splicing products of RNA precursors containing tandem duplications of the 5' or 3' splice sites of human beta-globin IVS 1 revealed that exon sequences play an important role in the relative use of the duplicated sites. These studies also show that the proximity of the 5' and 3' splice sites i...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(86)90343-0

    authors: Reed R,Maniatis T

    更新日期:1986-08-29 00:00:00

  • Crystallographic analysis of anti-p24 (HIV-1) monoclonal antibody cross-reactivity and polyspecificity.

    abstract::The X-ray crystal structures of an anti-p24 (HIV-1) monoclonal antibody Fab fragment alone and in complexes with the epitope peptide GATPQDLNTnL (n = norleucine), an epitope-homologous peptide GATPEDLNQKLAGN, as well as two unrelated peptides GLYEWGGARITNTD and efslkGpllqwrsG (D-peptide), are presented to a maximum re...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80469-9

    authors: Keitel T,Kramer A,Wessner H,Scholz C,Schneider-Mergener J,Höhne W

    更新日期:1997-12-12 00:00:00

  • Autophagy regulates programmed cell death during the plant innate immune response.

    abstract::The plant innate immune response includes the hypersensitive response (HR), a form of programmed cell death (PCD). PCD must be restricted to infection sites to prevent the HR from playing a pathologic rather than protective role. Here we show that plant BECLIN 1, an ortholog of the yeast and mammalian autophagy gene A...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2005.03.007

    authors: Liu Y,Schiff M,Czymmek K,Tallóczy Z,Levine B,Dinesh-Kumar SP

    更新日期:2005-05-20 00:00:00

  • A ligand-gated association between cytoplasmic domains of UNC5 and DCC family receptors converts netrin-induced growth cone attraction to repulsion.

    abstract::Netrins are bifunctional: they attract some axons and repel others. Netrin receptors of the Deleted in Colorectal Cancer (DCC) family are implicated in attraction and those of the UNC5 family in repulsion, but genetic evidence also suggests involvement of the DCC protein UNC-40 in some cases of repulsion. To test whet...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80804-1

    authors: Hong K,Hinck L,Nishiyama M,Poo MM,Tessier-Lavigne M,Stein E

    更新日期:1999-06-25 00:00:00

  • Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2.

    abstract::The initiation of chromosome segregation at anaphase is linked by the spindle assembly checkpoint to the completion of chromosome-microtubule attachment during metaphase. To determine the function of the mitotic checkpoint protein Mad2 during normal cell division and when mitosis goes awry, we have knocked out Mad2 in...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80875-2

    authors: Dobles M,Liberal V,Scott ML,Benezra R,Sorger PK

    更新日期:2000-06-09 00:00:00

  • High-Throughput Investigation of Diverse Junction Elements in RNA Tertiary Folding.

    abstract::RNAs fold into defined tertiary structures to function in critical biological processes. While quantitative models can predict RNA secondary structure stability, we are still unable to predict the thermodynamic stability of RNA tertiary structure. Here, we probe conformational preferences of diverse RNA two-way juncti...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2018.05.038

    authors: Denny SK,Bisaria N,Yesselman JD,Das R,Herschlag D,Greenleaf WJ

    更新日期:2018-07-12 00:00:00

  • Brain evolution and uniqueness in the human genome.

    abstract::Despite an ever-expanding database of sequenced mammalian genomes to be mined for clues, the emergence of the unique human brain remains an evolutionary enigma. In their new study, trawl the human genome and those of other mammals in search of short conserved DNA elements that show extremely rapid evolution only in hu...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.cell.2006.09.007

    authors: Amadio JP,Walsh CA

    更新日期:2006-09-22 00:00:00

  • Insertion of a multispanning membrane protein occurs sequentially and requires only one signal sequence.

    abstract::To study the insertion of multispanning membrane proteins into the endoplasmic reticulum, we constructed novel proteins on the cDNA level by repeating, up to four times, the internal signal-anchor domain of the asialoglycoprotein receptor H1. Upon in vitro translation in the presence of microsomes, these polypeptides ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(88)90009-8

    authors: Wessels HP,Spiess M

    更新日期:1988-10-07 00:00:00

  • Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein.

    abstract::X-linked lissencephaly and "double cortex" are allelic human disorders mapping to Xq22.3-Xq23 associated with arrest of migrating cerebral cortical neurons. We identified a novel 10 kb brain-specific cDNA interrupted by a balanced translocation in an XLIS patient that encodes a novel 40 kDa predicted protein named Dou...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80899-5

    authors: Gleeson JG,Allen KM,Fox JW,Lamperti ED,Berkovic S,Scheffer I,Cooper EC,Dobyns WB,Minnerath SR,Ross ME,Walsh CA

    更新日期:1998-01-09 00:00:00

  • Mosaic analysis with double markers reveals tumor cell of origin in glioma.

    abstract::Cancer cell of origin is difficult to identify by analyzing cells within terminal stage tumors, whose identity could be concealed by the acquired plasticity. Thus, an ideal approach to identify the cell of origin is to analyze proliferative abnormalities in distinct lineages prior to malignancy. Here, we use mosaic an...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2011.06.014

    authors: Liu C,Sage JC,Miller MR,Verhaak RG,Hippenmeyer S,Vogel H,Foreman O,Bronson RT,Nishiyama A,Luo L,Zong H

    更新日期:2011-07-22 00:00:00

  • A TRP channel that senses cold stimuli and menthol.

    abstract::A distinct subset of sensory neurons are thought to directly sense changes in thermal energy through their termini in the skin. Very little is known about the molecules that mediate thermoreception by these neurons. Vanilloid Receptor 1 (VR1), a member of the TRP family of channels, is activated by noxious heat. Here ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(02)00652-9

    authors: Peier AM,Moqrich A,Hergarden AC,Reeve AJ,Andersson DA,Story GM,Earley TJ,Dragoni I,McIntyre P,Bevan S,Patapoutian A

    更新日期:2002-03-08 00:00:00

  • Ras1 promotes cellular growth in the Drosophila wing.

    abstract::The Ras GTPase links extracellular mitogens to intracellular mechanisms that control cell proliferation. To understand how Ras regulates proliferation in vivo, we activated or inactivated Ras in cell clones in the developing Drosophila wing. Cells lacking Ras were smaller, had reduced growth rates, accumulated in G1, ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80679-0

    authors: Prober DA,Edgar BA

    更新日期:2000-02-18 00:00:00

  • A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation.

    abstract::Many proteins contain disordered regions of low-sequence complexity, which cause aging-associated diseases because they are prone to aggregate. Here, we study FUS, a prion-like protein containing intrinsically disordered domains associated with the neurodegenerative disease ALS. We show that, in cells, FUS forms liqui...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2015.07.047

    authors: Patel A,Lee HO,Jawerth L,Maharana S,Jahnel M,Hein MY,Stoynov S,Mahamid J,Saha S,Franzmann TM,Pozniakovski A,Poser I,Maghelli N,Royer LA,Weigert M,Myers EW,Grill S,Drechsel D,Hyman AA,Alberti S

    更新日期:2015-08-27 00:00:00

  • Protein disulfide isomerase acts as a redox-dependent chaperone to unfold cholera toxin.

    abstract::Cholera toxin is assembled from two subunits in the periplasm of Vibrio cholerae and disassembled in the analogous compartment of target cells, the lumen of the endoplasmic reticulum (ER), before a fragment of it, the A1 chain, is transported into the cytosol. We show that protein disulfide isomerase (PDI) in the ER l...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(01)00289-6

    authors: Tsai B,Rodighiero C,Lencer WI,Rapoport TA

    更新日期:2001-03-23 00:00:00

  • Identification of F-actin as the dynamic hub in a microbial-induced GTPase polarity circuit.

    abstract::Polarity in mammalian cells emerges from the assembly of signaling molecules into extensive biochemical interaction networks. Despite their complexity, bacterial pathogens have evolved parsimonious mechanisms to hijack these systems. Here, we develop a tractable experimental and theoretical model to uncover fundamenta...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2011.11.063

    authors: Orchard RC,Kittisopikul M,Altschuler SJ,Wu LF,Süel GM,Alto NM

    更新日期:2012-02-17 00:00:00

  • An exported peptide functions intracellularly to contribute to cell density signaling in B. subtilis.

    abstract::Competence development and sporulation in B. subtilis are partly controlled by peptides that accumulate in culture medium as cells grow to high density. We constructed two genes that encode mature forms of two different signaling molecules, the PhrA peptide that stimulates sporulation, and CSF, the competence- and spo...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(00)80277-9

    authors: Lazazzera BA,Solomon JM,Grossman AD

    更新日期:1997-06-13 00:00:00

  • An insertion sequence blocks the expression of a soybean lectin gene.

    abstract::We investigated soybean lectin gene structure and expression in a genetic line that accumulates normal levels of seed lectin (Le+ line) and in a mutant line that produces no detectable seed lectin (Le- line). We found that the nuclear and cytoplasmic lectin RNA levels in Le- embryos are approximately 0.01% of those in...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(83)90428-2

    authors: Goldberg RB,Hoschek G,Vodkin LO

    更新日期:1983-06-01 00:00:00

  • Mammalian prion biology: one century of evolving concepts.

    abstract::Prions have been responsible for an entire century of tragic episodes. Fifty years ago, kuru decimated the population of Papua New Guinea. Then, iatrogenic transmission of prions caused more than 250 cases of Creutzfeldt-Jakob disease. More recently, transmission of bovine spongiform encephalopathy to humans caused a ...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0092-8674(03)01031-6

    authors: Aguzzi A,Polymenidou M

    更新日期:2004-01-23 00:00:00

  • Replication Timing Becomes Intertwined with 3D Genome Organization.

    abstract::Through dissecting the link between spatial genome organization and DNA replication timing, Sima et al. (2018) discover early replicating control elements (ERCEs), a new type of cis-acting elements that regulate replication timing, transcription, and multiple layers of three-dimensional features of genome organization...

    journal_title:Cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.cell.2019.01.027

    authors: Ma J,Duan Z

    更新日期:2019-02-07 00:00:00

  • Ferroptosis: an iron-dependent form of nonapoptotic cell death.

    abstract::Nonapoptotic forms of cell death may facilitate the selective elimination of some tumor cells or be activated in specific pathological states. The oncogenic RAS-selective lethal small molecule erastin triggers a unique iron-dependent form of nonapoptotic cell death that we term ferroptosis. Ferroptosis is dependent up...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2012.03.042

    authors: Dixon SJ,Lemberg KM,Lamprecht MR,Skouta R,Zaitsev EM,Gleason CE,Patel DN,Bauer AJ,Cantley AM,Yang WS,Morrison B 3rd,Stockwell BR

    更新日期:2012-05-25 00:00:00

  • Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate.

    abstract::Four RNA products have been characterized from a soluble system that accurately splices purified substrate RNA. The labeled substrate RNA contained the first and second exons of the major late transcription unit of adenovirus 2 and a shortened form of the first intervening sequence. One of these RNA products was the e...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(84)90372-6

    authors: Grabowski PJ,Padgett RA,Sharp PA

    更新日期:1984-06-01 00:00:00

  • Analysis of homeodomain specificities allows the family-wide prediction of preferred recognition sites.

    abstract::We describe the comprehensive characterization of homeodomain DNA-binding specificities from a metazoan genome. The analysis of all 84 independent homeodomains from D. melanogaster reveals the breadth of DNA sequences that can be specified by this recognition motif. The majority of these factors can be organized into ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/j.cell.2008.05.023

    authors: Noyes MB,Christensen RG,Wakabayashi A,Stormo GD,Brodsky MH,Wolfe SA

    更新日期:2008-06-27 00:00:00

  • Interaction of fibronectin with its receptor on platelets.

    abstract::We report that the 12,000 dalton domain of fibronectin that interacts with fibroblast cell surfaces also binds specifically to thrombin-inducible, saturable receptors on platelets. Furthermore, we have used chemical cross-linking and monoclonal antibodies to show that the 12,000 dalton domain of fibronectin interacts ...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(85)90101-1

    authors: Gardner JM,Hynes RO

    更新日期:1985-09-01 00:00:00

  • Control of transcription through intragenic patterns of nucleosome composition.

    abstract::Several recent papers show that differences in histone modification and the use of histone variants at the 5' and 3' ends of genes influence the location and kinetics of transcriptional initiation. The ultimate target of most epigenetic mechanisms may be the regulation of nucleosome occupancy, which in turn controls a...

    journal_title:Cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.cell.2005.12.010

    authors: Lieb JD,Clarke ND

    更新日期:2005-12-29 00:00:00

  • Two amino acids within the knuckle of the first zinc finger specify DNA response element activation by the glucocorticoid receptor.

    abstract::The specificity of target gene activation by steroid receptors is encoded within a small, cysteine-rich domain that is believed to form two zinc-coordinated fingers. Here we show that the ability of glucocorticoid and estrogen receptors to discriminate between their closely related response elements resides in the two...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(89)90050-0

    authors: Danielsen M,Hinck L,Ringold GM

    更新日期:1989-06-30 00:00:00

  • Temporal regulation of salmonella virulence effector function by proteasome-dependent protein degradation.

    abstract::Salmonella enterica invasion of host cells requires the reversible activation of the Rho-family GTPases Cdc42 and Rac1 by the bacterially encoded GEF SopE and the GAP SptP, which exert their function at different times during infection and are delivered into host cells by a type III secretion system. We found that Sop...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/s0092-8674(03)00849-3

    authors: Kubori T,Galán JE

    更新日期:2003-10-31 00:00:00

  • Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells.

    abstract::Despite the obvious importance of epithelial stem cells in tissue homeostasis and tumorigenesis, little is known about their specific location or biological characteristics. Using 3H-thymidine labeling, we have identified a subpopulation of corneal epithelial basal cells, located in the peripheral cornea in a region c...

    journal_title:Cell

    pub_type: 杂志文章

    doi:10.1016/0092-8674(89)90958-6

    authors: Cotsarelis G,Cheng SZ,Dong G,Sun TT,Lavker RM

    更新日期:1989-04-21 00:00:00