Abstract：
:The mechanisms by which molecular chaperones assist quality control of cytosolic proteins are poorly understood. Analysis of the chaperone requirements for degradation of misfolded variants of a cytosolic protein, the VHL tumor suppressor, reveals that distinct chaperone pathways mediate its folding and quality control. While both folding and degradation of VHL require Hsp70, the chaperonin TRiC is essential for folding but is dispensable for degradation. Conversely, the chaperone Hsp90 neither participates in VHL folding nor is required to maintain misfolded VHL solubility but is essential for its degradation. The cochaperone HOP/Sti1p also participates in VHL quality control and may direct the triage decision by bridging the Hsp70-Hsp90 interaction. Our finding that a distinct chaperone complex is uniquely required for quality control provides evidence for active and specific chaperone participation in triage decisions and suggests that a hierarchy of chaperone interactions can control the alternate fates of a cytosolic protein.
journal_name
Celljournal_title
Cellauthors
McClellan AJ,Scott MD,Frydman Jdoi
10.1016/j.cell.2005.03.024subject
Has Abstractpub_date
2005-06-03 00:00:00pages
739-48issue
5eissn
0092-8674issn
1097-4172pii
S0092-8674(05)00296-5journal_volume
121pub_type
杂志文章相关文献
CELL文献大全abstract：:Two papers published in 1984 by the Varshavsky laboratory revealed that the ubiquitin/proteasome pathway is the principal system for degradation of short-lived proteins in mammalian cells, setting the stage for future demonstrations of this pathway's many regulatory roles. This perspective discusses the impact of thos...
journal_title：Cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s0092-8674(03)01074-2
更新日期：2004-01-23 00:00:00
abstract：:Evasion of DNA damage-induced cell death, via mutation of the p53 tumor suppressor or overexpression of prosurvival Bcl-2 family proteins, is a key step toward malignant transformation and therapeutic resistance. We report that depletion or acute inhibition of checkpoint kinase 1 (Chk1) is sufficient to restore gamma-...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.03.037
更新日期：2008-05-30 00:00:00
abstract：:The specific patterns and functional properties of electrical synapses of a nervous system are defined by the neuron-specific complement of electrical synapse constituents. We systematically examined the molecular composition of the electrical connectome of the nematode C. elegans through a genome- and nervous-system-...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.12.024
更新日期：2019-02-21 00:00:00
abstract：:Mitochondrial Ca(2+) (Ca(2+)(m)) uptake is mediated by an inner membrane Ca(2+) channel called the uniporter. Ca(2+) uptake is driven by the considerable voltage present across the inner membrane (ΔΨ(m)) generated by proton pumping by the respiratory chain. Mitochondrial matrix Ca(2+) concentration is maintained five ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.10.011
更新日期：2012-10-26 00:00:00
abstract：:While mutations affecting protein-coding regions have been examined across many cancers, structural variants at the genome-wide level are still poorly defined. Through integrative deep whole-genome and -transcriptome analysis of 101 castration-resistant prostate cancer metastases (109X tumor/38X normal coverage), we i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.06.039
更新日期：2018-07-26 00:00:00
abstract：:The axonal shafts of neurons contain bundled microtubules, whereas extending growth cones contain unbundled microtubule filaments, suggesting that localized activation of microtubule-associated proteins (MAP) at the transition zone may bundle these filaments during axonal growth. Dephosphorylation is thought to lead t...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.03.023
更新日期：2007-05-04 00:00:00
abstract：:When human lymphoblastoid mRNA was microinjected into X. laevis oocytes, titers of interferon rapidly reached a maximum inside the oocyte while accumulation of interferon continued in the incubation medium for at least 45 hr. If interferon protein was injected into oocytes it was rapidly inactivated. Significantly, ne...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(79)90260-5
更新日期：1979-07-01 00:00:00
abstract：:Partially edited mRNAs were selected by the polymerase chain reaction and sequenced. In the case of cytochrome b, 102 out of 106 clones displayed patterns of editing that were consistent with a strictly progressive 3' to 5' editing process, as predicted by the guide RNA model of RNA editing. In the case of cytochrome ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90197-m
更新日期：1990-06-01 00:00:00
abstract：:An attenuated form of familial adenomatous polyposis coli, AAPC, causes relatively few colonic polyps, but still carries a significant risk of colon cancer. The mutant alleles responsible for this attenuated phenotype have been mapped in several families to the adenomatous polyposis coli (APC) locus on human chromosom...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(93)90538-2
更新日期：1993-12-03 00:00:00
abstract：:22q11.2 microdeletions result in specific cognitive deficits and schizophrenia. Analysis of Df(16)A(+/-) mice, which model this microdeletion, revealed abnormalities in the formation of neuronal dendrites and spines, as well as altered brain microRNAs. Here, we show a drastic reduction of miR-185, which resides within...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.11.052
更新日期：2013-01-17 00:00:00
abstract：:Identifying the molecular mechanisms that underlie aging and their pharmacological manipulation are key aims for improving lifelong human health. Here, we identify a critical role for Ras-Erk-ETS signaling in aging in Drosophila. We show that inhibition of Ras is sufficient for lifespan extension downstream of reduced...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.06.023
更新日期：2015-07-02 00:00:00
abstract：:A key step in the regulation of transcription involves interactions between promoter-selective factors and various components of the transcriptional apparatus. Here we report the requirements for transcriptional activation directed by NTF-1, a developmentally regulated transcription factor in Drosophila. Reconstituted...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90019-2
更新日期：1991-08-09 00:00:00
abstract：:Polyglycylation is a posttranslational modification that generates glycine side chains on proteins. Here we identify a family of evolutionarily conserved glycine ligases that modify tubulin using different enzymatic mechanisms. In mammals, two distinct enzyme types catalyze the initiation and elongation steps of polyg...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.05.020
更新日期：2009-06-12 00:00:00
abstract：:Rosalind Franklin provided the key data for deriving the double helix structure of DNA. The English chemist also pioneered structural studies of colloids, viruses, and RNA. To celebrate the 100th anniversary of Franklin's birth, I summarize her work, which shaped the emerging discipline of molecular biology. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.07.028
更新日期：2020-08-20 00:00:00
abstract：:Ribonucleoproteins (RNPs) mediate key cellular functions such as gene expression and its regulation. Whereas most RNP enzymes are stable in composition and harbor preformed active sites, the spliceosome, which removes noncoding introns from precursor messenger RNAs (pre-mRNAs), follows fundamentally different strategi...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2009.02.009
更新日期：2009-02-20 00:00:00
abstract：:We have identified a nuclear pathway that rapidly degrades unspliced pre-mRNAs in yeast. This involves 3'-->5' degradation by the exosome complex and 5'-->3' degradation by the exonuclease Rat1p. 3'-->5' degradation is normally the major pathway and is regulated in response to carbon source. Inhibition of pre-mRNA deg...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00065-9
更新日期：2000-09-15 00:00:00
abstract：:Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically dead Cas9 lacking en...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2013.02.022
更新日期：2013-02-28 00:00:00
abstract：:Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C∗ complex) at an average resolution of 3.76 Å. The splicing factor Prp17...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.04.033
更新日期：2017-05-18 00:00:00
abstract：:Lipid composition affects the biophysical properties of membranes that provide a platform for receptor-mediated cellular signaling. To study the regulatory role of membrane lipid composition, we combined genetic perturbations of sphingolipid metabolism with the quantification of diverse steps in Toll-like receptor (TL...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.05.051
更新日期：2015-07-02 00:00:00
abstract：:T cells expressing chimeric antigen receptors (CARs) are promising cancer therapeutic agents, with the prospect of becoming the ultimate smart cancer therapeutics. To expand the capability of CAR T cells, here, we present a split, universal, and programmable (SUPRA) CAR system that simultaneously encompasses multiple ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.03.038
更新日期：2018-05-31 00:00:00
abstract：:The alpha IIb beta 3 integrin binds Arg-Gly-Asp-containing (RGD-containing) ligands in a cation-dependent interaction. A fourteen amino acid sequence, beta 3 (118-131), and an antibody to it, inhibited ligand binding functions of alpha IIb beta 3, and a 1:1 stoichiometric beta 3 (118-131)-RGD complex was detected by m...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90551-7
更新日期：1994-11-18 00:00:00
abstract：:In this issue, Baumann et al. (2007) identify a helicase PICH that localizes to "threads" that remain connected between sister kinetochores after they have separated in anaphase. These threads are thought to be catenated centromeric DNA. PICH contributes to the mitotic checkpoint by recruiting Mad2 to kinetochores and...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2006.12.022
更新日期：2007-01-12 00:00:00
abstract：:Clinical trials are key to translating scientific advances into progress in cancer research and care. Confronted by developments in basic science, the landscape of clinical cancer research is rapidly evolving. Here, we review recent changes in clinical trials' design and conduct, and we forecast future directions towa...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.01.027
更新日期：2017-02-09 00:00:00
abstract：:Anatomically modern humans interbred with Neanderthals and with a related archaic population known as Denisovans. Genomes of several Neanderthals and one Denisovan have been sequenced, and these reference genomes have been used to detect introgressed genetic material in present-day human genomes. Segments of introgres...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.02.031
更新日期：2018-03-22 00:00:00
abstract：:Viral infection causes the host to activate an antiviral response that, in part, is dependent on mitochondrial antiviral signaling protein (MAVS) to stimulate type I interferons. Zhang et al. (2019) demonstrate that glucose-generated lactate interacts with MAVS to suppress type I interferons. This study links glucose ...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2019.05.057
更新日期：2019-06-27 00:00:00
abstract：:Neurons in cortical circuits are often coactivated as ensembles, yet it is unclear whether ensembles play a functional role in behavior. Some ensemble neurons have pattern completion properties, triggering the entire ensemble when activated. Using two-photon holographic optogenetics in mouse primary visual cortex, we ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.05.045
更新日期：2019-07-11 00:00:00
abstract：:The germinal center (GC) reaction produces high-affinity antibodies by random mutation and selective clonal expansion of B cells with high-affinity receptors. The mechanism by which B cells are selected remains unclear, as does the role of the two anatomically defined areas of the GC, light zone (LZ) and dark zone (DZ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.10.032
更新日期：2010-11-12 00:00:00
abstract：:At the final step of homologous recombination, Holliday junction-containing joint molecules (JMs) are resolved to form crossover or noncrossover products. The enzymes responsible for JM resolution in vivo remain uncertain, but three distinct endonucleases capable of resolving JMs in vitro have been identified: Mus81-M...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.03.023
更新日期：2012-04-13 00:00:00
abstract：:Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogenei...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90440-2
更新日期：1981-03-01 00:00:00
abstract：:As the second dimension to the genome, the epigenome contains key information specific to every type of cells. Thousands of human epigenome maps have been produced in recent years thanks to rapid development of high throughput epigenome mapping technologies. In this review, we discuss the current epigenome mapping too...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/j.cell.2013.09.011
更新日期：2013-09-26 00:00:00
