Abstract：
:The germinal center (GC) reaction produces high-affinity antibodies by random mutation and selective clonal expansion of B cells with high-affinity receptors. The mechanism by which B cells are selected remains unclear, as does the role of the two anatomically defined areas of the GC, light zone (LZ) and dark zone (DZ). We combined a transgenic photoactivatable fluorescent protein tracer with multiphoton laser-scanning microscopy and flow cytometry to examine anatomically defined LZ and DZ B cells and GC selection. We find that B cell division is restricted to the DZ, with a net vector of B cell movement from the DZ to the LZ. The decision to return to the DZ and undergo clonal expansion is controlled by T helper cells in the GC LZ, which discern between LZ B cells based on the amount of antigen captured and presented. Thus, T cell help, and not direct competition for antigen, is the limiting factor in GC selection.
journal_name
Celljournal_title
Cellauthors
Victora GD,Schwickert TA,Fooksman DR,Kamphorst AO,Meyer-Hermann M,Dustin ML,Nussenzweig MCdoi
10.1016/j.cell.2010.10.032subject
Has Abstractpub_date
2010-11-12 00:00:00pages
592-605issue
4eissn
0092-8674issn
1097-4172pii
S0092-8674(10)01236-5journal_volume
143pub_type
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