Abstract:
:Deletion analysis was carried out on the human 7SK RNA gene to map regions essential for in vitro transcription by RNA polymerase III. The sequence promoting transcription is located between 37 and 3 bp upstream of the 7SK RNA coding region. RNA polymerase III transcription of adjacent plasmid sequences can be directed by this promoter in the complete absence of the 7SK RNA coding region, indicating that no internal promoter sequences are required. Transcription is terminated by a stretch of T residues, typical of RNA polymerase III transcription. The promoter contains a TATA box at position -25, mutations within which dramatically reduce the efficiency of transcription. Upstream sequences from position -37 to -243 increase the promoter's efficiency. The promoter recognized by RNA polymerase III is structurally and functionally similar to the promoter of genes transcribed by RNA polymerase II.
journal_name
Celljournal_title
Cellauthors
Murphy S,Di Liegro C,Melli Mdoi
10.1016/0092-8674(87)90012-2subject
Has Abstractpub_date
1987-10-09 00:00:00pages
81-7issue
1eissn
0092-8674issn
1097-4172pii
0092-8674(87)90012-2journal_volume
51pub_type
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