The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.

Abstract:

:Deletion analysis was carried out on the human 7SK RNA gene to map regions essential for in vitro transcription by RNA polymerase III. The sequence promoting transcription is located between 37 and 3 bp upstream of the 7SK RNA coding region. RNA polymerase III transcription of adjacent plasmid sequences can be directed by this promoter in the complete absence of the 7SK RNA coding region, indicating that no internal promoter sequences are required. Transcription is terminated by a stretch of T residues, typical of RNA polymerase III transcription. The promoter contains a TATA box at position -25, mutations within which dramatically reduce the efficiency of transcription. Upstream sequences from position -37 to -243 increase the promoter's efficiency. The promoter recognized by RNA polymerase III is structurally and functionally similar to the promoter of genes transcribed by RNA polymerase II.

journal_name

Cell

journal_title

Cell

authors

Murphy S,Di Liegro C,Melli M

doi

10.1016/0092-8674(87)90012-2

subject

Has Abstract

pub_date

1987-10-09 00:00:00

pages

81-7

issue

1

eissn

0092-8674

issn

1097-4172

pii

0092-8674(87)90012-2

journal_volume

51

pub_type

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