The in vitro transcription of the 7SK RNA gene by RNA polymerase III is dependent only on the presence of an upstream promoter.


:Deletion analysis was carried out on the human 7SK RNA gene to map regions essential for in vitro transcription by RNA polymerase III. The sequence promoting transcription is located between 37 and 3 bp upstream of the 7SK RNA coding region. RNA polymerase III transcription of adjacent plasmid sequences can be directed by this promoter in the complete absence of the 7SK RNA coding region, indicating that no internal promoter sequences are required. Transcription is terminated by a stretch of T residues, typical of RNA polymerase III transcription. The promoter contains a TATA box at position -25, mutations within which dramatically reduce the efficiency of transcription. Upstream sequences from position -37 to -243 increase the promoter's efficiency. The promoter recognized by RNA polymerase III is structurally and functionally similar to the promoter of genes transcribed by RNA polymerase II.






Murphy S,Di Liegro C,Melli M




Has Abstract


1987-10-09 00:00:00
















  • Nucleoporins directly stimulate expression of developmental and cell-cycle genes inside the nucleoplasm.

    abstract::Nuclear pore complexes (NPCs) mediate transport across the nuclear envelope. In yeast, they also interact with active genes, attracting or retaining them at the nuclear periphery. In higher eukaryotes, some NPC components (nucleoporins) are also found in the nucleoplasm, with a so far unknown function. We have functio...


    pub_type: 杂志文章


    authors: Kalverda B,Pickersgill H,Shloma VV,Fornerod M

    更新日期:2010-02-05 00:00:00

  • Facilitated recycling pathway for RNA polymerase III.

    abstract::We show that the high in vitro transcription efficiency of yeast RNA pol III is mainly due to rapid recycling. Kinetic analysis shows that RNA polymerase recycling on preassembled tDNA.TFIIIC.TFIIIB complexes is much faster than the initial transcription cycle. High efficiency of RNA pol III recycling is favored at hi...


    pub_type: 杂志文章


    authors: Dieci G,Sentenac A

    更新日期:1996-01-26 00:00:00

  • A precursor to a minor species of yeast tRNASer contains an intervening sequence.

    abstract::Certain tRNAs in S. cerevisiae (tRNATyr and tRNAPhe) arise via precursor molecules which are mature at the 5' and 3' termini but contain intervening sequences adjacent to the anticodon (Knapp et al., 1978; O'Farrell et al., 1978). In addition to these molecules, precursors to several other tRNAs accumulate in a temper...


    pub_type: 杂志文章


    authors: Etcheverry T,Colby D,Guthrie C

    更新日期:1979-09-01 00:00:00

  • Species-dependent posttranscriptional regulation of NOS1 by FMRP in the developing cerebral cortex.

    abstract::Fragile X syndrome (FXS), the leading monogenic cause of intellectual disability and autism, results from loss of function of the RNA-binding protein FMRP. Here, we show that FMRP regulates translation of neuronal nitric oxide synthase 1 (NOS1) in the developing human neocortex. Whereas NOS1 mRNA is widely expressed, ...


    pub_type: 杂志文章


    authors: Kwan KY,Lam MM,Johnson MB,Dube U,Shim S,Rašin MR,Sousa AM,Fertuzinhos S,Chen JG,Arellano JI,Chan DW,Pletikos M,Vasung L,Rowitch DH,Huang EJ,Schwartz ML,Willemsen R,Oostra BA,Rakic P,Heffer M,Kostović I,Judaš M,

    更新日期:2012-05-11 00:00:00

  • Sequential E2s drive polyubiquitin chain assembly on APC targets.

    abstract::The anaphase-promoting complex (APC), or cyclosome, is an E3 ubiquitin-protein ligase that collaborates with E2 ubiquitin-conjugating enzymes to assemble polyubiquitin chains on proteins important for cell-cycle progression. It remains unclear how the APC - or many other E3s - promotes the multiple distinct reactions ...


    pub_type: 杂志文章


    authors: Rodrigo-Brenni MC,Morgan DO

    更新日期:2007-07-13 00:00:00

  • Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System.

    abstract::During neural tube closure and spinal cord development, many cells die in both the central and peripheral nervous systems (CNS and PNS, respectively). However, myeloid-derived professional phagocytes have not yet colonized the trunk region during early neurogenesis. How apoptotic cells are removed from this region dur...


    pub_type: 杂志文章


    authors: Zhu Y,Crowley SC,Latimer AJ,Lewis GM,Nash R,Kucenas S

    更新日期:2019-09-19 00:00:00

  • Regulatory nascent peptides in the ribosomal tunnel.

    abstract::Accumulating evidence for nascent-peptide-mediated regulation of translation suggests that all nascent peptides do not necessarily interact with the ribosome in a similar manner. Recent studies have helped to elucidate the exit route of the nascent chain and its interactions with the ribosome. ...


    pub_type: 杂志文章,评审


    authors: Tenson T,Ehrenberg M

    更新日期:2002-03-08 00:00:00

  • Single-Molecule Visualization of MCM2-7 DNA Loading: Seeing Is Believing.

    abstract::The first event in the initiation of eukaryotic DNA replication is the recruitment of the MCM2-7 ATPase, the core of the replicative DNA helicase, to origins. Ticau et al. use single-molecule imaging to reveal how ORC, Cdc6, and Cdt1 cooperate to load MCM2-7 onto DNA, enabling bidirectional replication. ...


    pub_type: 评论,杂志文章


    authors: Chistol G,Walter JC

    更新日期:2015-04-23 00:00:00

  • Aire, master of many trades.

    abstract::In thymic epithelial cells, the protein Aire (autoimmune regulator) induces the ectopic expression of hundreds of peripheral tissue antigens, thus enlarging the repertoire of antigens available for the induction of central T cell tolerance. By analyzing Aire's interacting partners, Abramson et al. (2010) shed new ligh...


    pub_type: 评论,杂志文章


    authors: Kyewski B,Peterson P

    更新日期:2010-01-08 00:00:00

  • The excision of intervening sequences from Salmonella 23S ribosomal RNA.

    abstract::Novel, approximately 90 bp intervening sequences (IVs) were discovered within the 23S rRNA genes of S. typhimurium and S. arizonae. These non-rRNA sequences are transcribed and then excised during rRNA maturation. The rRNA fragments that result from the excision of the extra sequences are not religated. This results i...


    pub_type: 杂志文章


    authors: Burgin AB,Parodos K,Lane DJ,Pace NR

    更新日期:1990-02-09 00:00:00

  • BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis.

    abstract::Circadian rhythms control organismal physiology throughout the day. At the cellular level, clock regulation is established by a self-sustained Bmal1-dependent transcriptional oscillator network. However, it is still unclear how different tissues achieve a synchronized rhythmic physiology. That is, do they respond inde...


    pub_type: 杂志文章


    authors: Welz PS,Zinna VM,Symeonidi A,Koronowski KB,Kinouchi K,Smith JG,Guillén IM,Castellanos A,Furrow S,Aragón F,Crainiciuc G,Prats N,Caballero JM,Hidalgo A,Sassone-Corsi P,Benitah SA

    更新日期:2019-05-30 00:00:00

  • Deconvolving the recognition of DNA shape from sequence.

    abstract::Protein-DNA binding is mediated by the recognition of the chemical signatures of the DNA bases and the 3D shape of the DNA molecule. Because DNA shape is a consequence of sequence, it is difficult to dissociate these modes of recognition. Here, we tease them apart in the context of Hox-DNA binding by mutating residues...


    pub_type: 杂志文章


    authors: Abe N,Dror I,Yang L,Slattery M,Zhou T,Bussemaker HJ,Rohs R,Mann RS

    更新日期:2015-04-09 00:00:00

  • COP1, an Arabidopsis regulatory gene, encodes a protein with both a zinc-binding motif and a G beta homologous domain.

    abstract::Plant seedling development is capable of following 1 of 2 distinct morphogenic pathways: skotomorphogenesis in darkness and photomorphogenesis in light. Dark-grown Arabidopsis seedlings with recessive mutations at the constitutively photomorphogenic (COP1) locus indicate that the wild-type COP1 protein represses photo...


    pub_type: 杂志文章


    authors: Deng XW,Matsui M,Wei N,Wagner D,Chu AM,Feldmann KA,Quail PH

    更新日期:1992-11-27 00:00:00

  • Ubiquitin is attached to membranes of baculovirus particles by a novel type of phospholipid anchor.

    abstract::Purified budded virions of Autographa californica nuclear polyhedrosis virus (AcNPV) contain abundant amounts of free ubiquitin, which has an altered electrophoretic mobility on SDS gels as compared with standard ubiquitin. Phase extraction of virion proteins with Triton X-114 indicated that the modified form of ubiqu...


    pub_type: 杂志文章


    authors: Guarino LA,Smith G,Dong W

    更新日期:1995-01-27 00:00:00

  • Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.

    abstract::Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here we offer an in-depth analysis of the transcriptional response to SARS-CoV-2 comp...


    pub_type: 杂志文章


    authors: Blanco-Melo D,Nilsson-Payant BE,Liu WC,Uhl S,Hoagland D,Møller R,Jordan TX,Oishi K,Panis M,Sachs D,Wang TT,Schwartz RE,Lim JK,Albrecht RA,tenOever BR

    更新日期:2020-05-28 00:00:00

  • The genetic basis for specific anosmia to isovaleric acid in the mouse.

    abstract::The detection and discrimination of odorants in mammals is thought to be mediated by a family of 100-1000 seven transmembrane domain receptor proteins, although none of these putative olfactory receptors have been shown to bind individual odorants with high affinity. We have used a genetic approach to identify the gen...


    pub_type: 杂志文章


    authors: Griff IC,Reed RR

    更新日期:1995-11-03 00:00:00

  • Allele-Specific HLA Loss and Immune Escape in Lung Cancer Evolution.

    abstract::Immune evasion is a hallmark of cancer. Losing the ability to present neoantigens through human leukocyte antigen (HLA) loss may facilitate immune evasion. However, the polymorphic nature of the locus has precluded accurate HLA copy-number analysis. Here, we present loss of heterozygosity in human leukocyte antigen (L...


    pub_type: 杂志文章


    authors: McGranahan N,Rosenthal R,Hiley CT,Rowan AJ,Watkins TBK,Wilson GA,Birkbak NJ,Veeriah S,Van Loo P,Herrero J,Swanton C,TRACERx Consortium.

    更新日期:2017-11-30 00:00:00

  • EGFR-mediated Beclin 1 phosphorylation in autophagy suppression, tumor progression, and tumor chemoresistance.

    abstract::Cell surface growth factor receptors couple environmental cues to the regulation of cytoplasmic homeostatic processes, including autophagy, and aberrant activation of such receptors is a common feature of human malignancies. Here, we defined the molecular basis by which the epidermal growth factor receptor (EGFR) tyro...


    pub_type: 杂志文章


    authors: Wei Y,Zou Z,Becker N,Anderson M,Sumpter R,Xiao G,Kinch L,Koduru P,Christudass CS,Veltri RW,Grishin NV,Peyton M,Minna J,Bhagat G,Levine B

    更新日期:2013-09-12 00:00:00

  • Green genes-comparative genomics of the green branch of life.

    abstract::As more plant genome sequences become available, researchers are increasingly using comparative genomics to address some of the major questions in plant biology. Such questions include the evolution of photosynthesis and multicellularity, the developmental genetic changes responsible for alterations in body plan, and ...


    pub_type: 杂志文章


    authors: Bowman JL,Floyd SK,Sakakibara K

    更新日期:2007-04-20 00:00:00

  • Accelerated evolution of nervous system genes in the origin of Homo sapiens.

    abstract::Human evolution is characterized by a dramatic increase in brain size and complexity. To probe its genetic basis, we examined the evolution of genes involved in diverse aspects of nervous system biology. We found that these genes display significantly higher rates of protein evolution in primates than in rodents. Impo...


    pub_type: 杂志文章


    authors: Dorus S,Vallender EJ,Evans PD,Anderson JR,Gilbert SL,Mahowald M,Wyckoff GJ,Malcom CM,Lahn BT

    更新日期:2004-12-29 00:00:00

  • Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription.

    abstract::Many cofactors bind the hormone-activated estrogen receptor (ER), yet the specific regulators of endogenous ER-mediated gene transcription are unknown. Using chromatin immunoprecipitation (ChIP), we find that ER and a number of coactivators rapidly associate with estrogen responsive promoters following estrogen treatm...


    pub_type: 杂志文章


    authors: Shang Y,Hu X,DiRenzo J,Lazar MA,Brown M

    更新日期:2000-12-08 00:00:00

  • Abelson leukemia virus induces lymphoid and erythroid colonies in infected fetal cell cultures.

    abstract::An examination of Abelson murine leukemia virus (A-MuL V)-hematopoietic cell interaction in cultures of fetal tissues reveals that A-MuLV can stimulate the formation of two different types of colonies. One type of colony is white and composed of A-MuLV-transformed lymphoid cells that can develop into established cell ...


    pub_type: 杂志文章


    authors: Waneck GL,Rosenberg N

    更新日期:1981-10-01 00:00:00

  • Different pathways of differentiation of pre-B cell lines are induced by dendritic cells and T cells from different lymphoid tissues.

    abstract::Nontransformed pre-B cells were induced to differentiate in vitro along several different but predictable pathways with only dendritic cells (DC) and concanavalin A-stimulated T lymphocytes. DC-T from spleen induced secretion of only IgM, whereas DC-T from Peyer's patches induced high levels of IgA and intermediate le...


    pub_type: 杂志文章


    authors: Spalding DM,Griffin JA

    更新日期:1986-02-14 00:00:00

  • A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes.

    abstract::The thymus exports a selected subset of virgin T lymphocytes to the peripheral lymphoid organs. The mature phenotype of these thymus emigrants is similar to that of medullary thymocytes and has been cited as supporting a medullary rather than cortical exit site. Using the monoclonal antibody MEL-14, we identify a 1%-3...


    pub_type: 杂志文章


    authors: Reichert RA,Gallatin WM,Butcher EC,Weissman IL

    更新日期:1984-08-01 00:00:00

  • Secretin-Activated Brown Fat Mediates Prandial Thermogenesis to Induce Satiation.

    abstract::The molecular mediator and functional significance of meal-associated brown fat (BAT) thermogenesis remains elusive. Here, we identified the gut hormone secretin as a non-sympathetic BAT activator mediating prandial thermogenesis, which consequentially induces satiation, thereby establishing a gut-secretin-BAT-brain a...


    pub_type: 杂志文章


    authors: Li Y,Schnabl K,Gabler SM,Willershäuser M,Reber J,Karlas A,Laurila S,Lahesmaa M,U Din M,Bast-Habersbrunner A,Virtanen KA,Fromme T,Bolze F,O'Farrell LS,Alsina-Fernandez J,Coskun T,Ntziachristos V,Nuutila P,Klingenspor M

    更新日期:2018-11-29 00:00:00

  • ATP-dependent fusion of liposomes with the Golgi apparatus of perforated cells.

    abstract::We examined the interactions of lipid vesicles (liposomes) labeled with various fluorescent markers with the intracellular membranes of semi-intact ("perforated") fibroblasts. When incubations were performed in the presence of an ATP-regenerating system, both vesicle lipids and entrapped water soluble markers were tra...


    pub_type: 杂志文章


    authors: Kobayashi T,Pagano RE

    更新日期:1988-12-02 00:00:00

  • Acetylation in histone H3 globular domain regulates gene expression in yeast.

    abstract::In Saccharomyces cerevisiae, known histone acetylation sites regulating gene activity are located in the N-terminal tails protruding from the nucleosome core. We report lysine 56 in histone H3 as a novel acetylation site that is located in the globular domain, where it extends toward the DNA major groove at the entry-...


    pub_type: 杂志文章


    authors: Xu F,Zhang K,Grunstein M

    更新日期:2005-05-06 00:00:00

  • Endosome-specific localization and function of the ARF activator GNOM.

    abstract::ARF GTPases act at multiple steps of the secretory and vacuolar/lysosomal trafficking pathways, but little is known about the spatial regulation of ARF activation. In this issue of Cell, Geldner et al. demonstrate that the Arabidopsis ARF activator GNOM localizes to endosomes where it controls the polarized traffickin...


    pub_type: 评论,杂志文章


    authors: Bonifacino JS,Jackson CL

    更新日期:2003-01-24 00:00:00

  • Observing a DNA polymerase choose right from wrong.

    abstract::DNA polymerase (pol) β is a model polymerase involved in gap-filling DNA synthesis utilizing two metals to facilitate nucleotidyl transfer. Previous structural studies have trapped catalytic intermediates by utilizing substrate analogs (dideoxy-terminated primer or nonhydrolysable incoming nucleotide). To identify add...


    pub_type: 杂志文章


    authors: Freudenthal BD,Beard WA,Shock DD,Wilson SH

    更新日期:2013-07-03 00:00:00

  • Histone deimination antagonizes arginine methylation.

    abstract::Methylation of arginine residues within histone H3 has been linked to active transcription. This modification appears on the estrogen-regulated pS2 promoter when the CARM1 methyltransferase is recruited during transcriptional activation. Here we describe a process, deimination, that converts histone arginine to citrul...


    pub_type: 杂志文章


    authors: Cuthbert GL,Daujat S,Snowden AW,Erdjument-Bromage H,Hagiwara T,Yamada M,Schneider R,Gregory PD,Tempst P,Bannister AJ,Kouzarides T

    更新日期:2004-09-03 00:00:00