Abstract：
:Exit from mitosis must not occur prior to partitioning of chromosomes between daughter cells. We find that the GTP binding protein Tem1, a regulator of mitotic exit, is present on the spindle pole body that migrates into the bud during S phase and mitosis. Tem1's exchange factor, Lte1, localizes to the bud. Thus, Tem1 and Lte1 are present in the same cellular compartment (the bud) only after the nucleus enters the bud during nuclear division. We also find that the presence of Tem1 and Lte1 in the bud is required for mitotic exit. Our results suggest that the spatial segregation of Tem1 and Lte1 ensures that exit from mitosis only occurs after the genetic material is partitioned between mother and daughter cell.
journal_name
Celljournal_title
Cellauthors
Bardin AJ,Visintin R,Amon Adoi
10.1016/s0092-8674(00)00007-6subject
Has Abstractpub_date
2000-07-07 00:00:00pages
21-31issue
1eissn
0092-8674issn
1097-4172pii
S0092-8674(00)00007-6journal_volume
102pub_type
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