Abstract：
:Chimeric histocompatibility genes encoding the amino-terminal (beta 1) domain of the class II Ak beta polypeptide and the carboxy-terminal (C2, transmembrane, and intracytoplasmic) domains of either the class I H-2Ld or H-2Dd molecules were stably introduced into mouse L cells. Although both were transcribed, only 5' Ak beta/3' H-2Dd transformants had significant cell membrane expression of a 30-40 kd, heterogeneous glycoprotein containing Ak beta 1 and H-2Dd (C2) serological epitopes. These transformants had a unique pattern of reactivity with monoclonal antibodies previously identified as requiring the Ak beta 1 domain for recognition of complete I-A molecules. These results allow new insight into the structural requirements for cell surface expression of proteins and provide unique cellular reagents for the dissection of humoral and cell-mediated recognition of MHC molecules.
journal_name
Celljournal_title
Cellauthors
McCluskey J,Germain RN,Margulies DHdoi
10.1016/0092-8674(85)90139-4subject
Has Abstractpub_date
1985-02-01 00:00:00pages
247-57issue
2eissn
0092-8674issn
1097-4172pii
0092-8674(85)90139-4journal_volume
40pub_type
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