Abstract：
:The primer transcript of plasmid ColE1 extends beyond the replication origin in either of two different modes. It does or does not form a hybrid with the template DNA. When a stretch of 20 deoxyadenosine residues is inserted into the template strand downstream of the origin, more than 90% of hybridized transcripts and about 10% of unhybridized transcripts terminate at the insert. When the number of inserted residues is reduced to ten, the corresponding values are decreased considerably, while the sites of termination are almost unchanged. A palindrome immediately before the stretch increased the efficiency of termination of unhybridized transcripts. Upon termination of hybridized transcripts, RNA polymerase detaches from the template. A structure made by a hybrid or by folded RNA may affect a property of transcription, and a stretch of deoxyadenosine residues including those beyond the actual site of termination may facilitate detachment of RNA polymerase from the template DNA.
journal_name
Celljournal_title
Cellauthors
Tomizawa J,Masukata Hdoi
10.1016/0092-8674(87)90131-0subject
Has Abstractpub_date
1987-11-20 00:00:00pages
623-30issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(87)90131-0journal_volume
51pub_type
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