Abstract:
:The Polycomb group (Pc-G) proteins are responsible for keeping developmental regulators, like homeotic genes, stably and inheritably repressed during Drosophila development. Several similarities to a protein class involved in heterochromatin formation suggest that the Pc-G exerts its function at the higher order chromatin level. Here we have mapped the distribution of the Pc protein in the homeotic bithorax complex (BX-C) of Drosophila tissue culture cells. We have elaborated a method, based on the in vivo formaldehyde cross-linking technique, that allows a substantial enrichment for Pc-interacting sites by immunoprecipitation of the cross-linked chromatin with anti-Pc antibodies. We find that the Pc protein quantitatively covers large regulatory regions of repressed BX-C genes. Conversely, we find that the Abdominal-B gene is active in these cells and the region devoid of any bound Pc protein.
journal_name
Celljournal_title
Cellauthors
Orlando V,Paro Rdoi
10.1016/0092-8674(93)90328-nsubject
Has Abstractpub_date
1993-12-17 00:00:00pages
1187-98issue
6eissn
0092-8674issn
1097-4172pii
0092-8674(93)90328-Njournal_volume
75pub_type
杂志文章相关文献
CELL文献大全abstract::Subsets of long-lived, tumor-initiating stem cells often escape cancer therapies. However, sources and mechanisms that generate tumor heterogeneity and drug-resistant cell population are still unfolding. Here, we devise a functional reporter system to lineage trace and/or genetic ablate signaling in TGF-β-activated sq...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.01.043
更新日期:2015-02-26 00:00:00
abstract::Subcellular compartments in which folding and assembly of proteins occur seem to have a set of PCB proteins capable of mediating these and related processes, such as translocation across membranes. When a domain of a polypeptide chain emerges from a ribosome during synthesis or from the distal side of a membrane durin...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/0092-8674(89)90005-6
更新日期:1989-11-17 00:00:00
abstract::The quest to slow aging has come far, and what used to be the domain of science fiction writers and snake oil salesmen may soon become science fact. Innovative new approaches, such as the use of the very short-lived African killifish (Harel et al.), are bridging the translational gap and bring the promise of healthy l...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2015.02.026
更新日期:2015-02-26 00:00:00
abstract::Exchange factors for ARF GTPases (ARF-GEFs) regulate vesicle trafficking in a variety of organisms. The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of the plant hormone auxin. Mutations in GNOM lead to development...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00003-5
更新日期:2003-01-24 00:00:00
abstract::Mouse macrophages and lymphocytes express two distinct isoforms of a single class of Fc receptor for IgG. The macrophage isoform (FcRII-B2) is identical to the lymphocyte isoform (FcRII-B1) except for an inframe insertion in the cytoplasmic tail of FcRII-B1 that increases its length from 47 to 94 amino acids. To deter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90846-5
更新日期:1989-07-28 00:00:00
abstract::Oligodendrocyte-specific protein (OSP)/claudin-11 is a recently identified transmembrane protein found in CNS myelin and testis with unknown function. Herein we demonstrate that Osp null mice exhibit both neurological and reproductive deficits: CNS nerve conduction is slowed, hindlimb weakness is conspicuous, and male...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81553-6
更新日期:1999-12-10 00:00:00
abstract::Phosphoinositide 3 kinase (PI3K) is a key signaling enzyme implicated in receptor-stimulated mitogenesis, oxidative bursting in neutrophils, membrane ruffling, and glucose uptake. A PI3K has already been purified, cloned, and shown to be regulated by receptors that act via tyrosine kinase-dependent regulatory mechanis...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90237-2
更新日期:1994-04-08 00:00:00
abstract::Certain mutations can cause proteins to accumulate in neurons, leading to neurodegeneration. We recently showed, however, that upregulation of a wild-type protein, Ataxin1, caused by haploinsufficiency of its repressor, the RNA-binding protein Pumilio1 (PUM1), also causes neurodegeneration in mice. We therefore search...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.02.006
更新日期:2018-02-22 00:00:00
abstract::Antibodies capable of neutralizing divergent influenza A viruses could form the basis of a universal vaccine. Here, from subjects enrolled in an H5N1 DNA/MIV-prime-boost influenza vaccine trial, we sorted hemagglutinin cross-reactive memory B cells and identified three antibody classes, each capable of neutralizing di...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.06.043
更新日期:2016-07-28 00:00:00
abstract::Several recent papers show that differences in histone modification and the use of histone variants at the 5' and 3' ends of genes influence the location and kinetics of transcriptional initiation. The ultimate target of most epigenetic mechanisms may be the regulation of nucleosome occupancy, which in turn controls a...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2005.12.010
更新日期:2005-12-29 00:00:00
abstract::FtsK acts at the bacterial division septum to couple chromosome segregation with cell division. We demonstrate that a truncated FtsK derivative, FtsK(50C), uses ATP hydrolysis to translocate along duplex DNA as a multimer in vitro, consistent with FtsK having an in vivo role in pumping DNA through the closing division...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)00624-4
更新日期:2002-01-25 00:00:00
abstract::Using single-cell RNA sequencing, Avraham et al. investigate how variability in macrophage response to infection is controlled by variability within the pathogen population. They find that heterogeneous expression of the Salmonella virulence factor PhoP and subsequent cell-wall modifications lead to the bimodal induct...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2015.08.053
更新日期:2015-09-10 00:00:00
abstract::The liver is capable of completely regenerating itself in response to injury and after partial hepatectomy. In liver of old animals, the proliferative response is dramatically reduced, the mechanism for which is unknown. The liver specific protein, C/EBPalpha, normally arrests proliferation of hepatocytes through inhi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00318-0
更新日期:2003-05-16 00:00:00
abstract::Human evolution is characterized by a dramatic increase in brain size and complexity. To probe its genetic basis, we examined the evolution of genes involved in diverse aspects of nervous system biology. We found that these genes display significantly higher rates of protein evolution in primates than in rodents. Impo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2004.11.040
更新日期:2004-12-29 00:00:00
abstract::Homologous recombination in embryonal stem cells has been used to produce a fusion oncogene, thereby mimicking chromosomal translocations that frequently result in formation of tumor-specific fusion oncogenes in human malignancies. AF9 sequences were fused into the mouse Mll gene so that expression of the Mll-AF9 fusi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81269-6
更新日期:1996-06-14 00:00:00
abstract::During the postpartum period, the brain's inhibitory GABAA receptors may not recover in time following their reduced numbers during pregnancy. This is likely the cause of postpartum depression prevalent in ∼12% of childbearing women. A new therapy for this condition consists of administering a synthetic neurosteroid d...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.12.016
更新日期:2019-01-10 00:00:00
abstract::Peptide hormone fibroblast growth factor-21 (FGF21) has insulin-mimetic properties. Dutchak et al. now suggest that FGF21 also acts in an autocrine fashion in adipocytes and is required to mediate effects of the PPARγ agonist class of antidiabetic drugs. Does this new property improve FGF21's fledgling clinical prospe...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2012.01.020
更新日期:2012-02-03 00:00:00
abstract::The Ion gene of E. coli controls the stability of two bacteriophage lambda proteins. The functional half-life of the phage N gene product, measured by complementation, is increased about 5-fold in Ion mutant strains, from 2 min to 10 min. The chemical half-life of N protein, determined by its disappearance on polyacry...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90518-3
更新日期:1981-04-01 00:00:00
abstract::Analysis of the in vitro splicing products of RNA precursors containing tandem duplications of the 5' or 3' splice sites of human beta-globin IVS 1 revealed that exon sequences play an important role in the relative use of the duplicated sites. These studies also show that the proximity of the 5' and 3' splice sites i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90343-0
更新日期:1986-08-29 00:00:00
abstract::The mesenchymal-amoeboid transition (MAT) was proposed as a mechanism for cancer cells to adapt their migration mode to their environment. While the molecular pathways involved in this transition are well documented, the role of the microenvironment in the MAT is still poorly understood. Here, we investigated how conf...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.01.007
更新日期:2015-02-12 00:00:00
abstract::Dysregulation of splicing and alternative splicing underlies many genetic and acquired diseases. We present an overview of recent strategies and successes in modulating splicing therapeutically in clinical and preclinical contexts. Effective approaches include restoring open reading frames, influencing alternative spl...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2012.02.014
更新日期:2012-03-16 00:00:00
abstract::It is unknown whether the activity of the nervous system can be inherited. In Caenorhabditis elegans nematodes, parental responses can transmit heritable small RNAs that regulate gene expression transgenerationally. In this study, we show that a neuronal process can impact the next generations. Neurons-specific synthe...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.04.029
更新日期:2019-06-13 00:00:00
abstract::Mitochondrial respiratory-chain complexes assemble from subunits of dual genetic origin assisted by specialized assembly factors. Whereas core subunits are translated on mitochondrial ribosomes, others are imported after cytosolic translation. How imported subunits are ushered to assembly intermediates containing mito...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.11.053
更新日期:2012-12-21 00:00:00
abstract::Thrombin stimulates 32Pi incorporation into phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol 4,5-bis-phosphate (PIP2), and phosphatidylinositol (PI), and initiates DNA synthesis in hamster (NIL) fibroblasts at a half-maximal concentration of 125 ng/ml. Neomycin, which binds PIP2 and PIP, inhibits both thro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90105-9
更新日期:1985-09-01 00:00:00
abstract::Lower urinary tract infections are among the most common human bacterial infections, but extension to the kidneys is rare. This has been attributed to mechanical forces, such as urine flow, that prevent the ascent of bladder microbes. Here, we show that the regional hypersalinity, required for the kidney's urine-conce...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.07.022
更新日期:2017-08-24 00:00:00
abstract::We show that expression of the iso-1-cytochrome c gene of Saccharomyces cerevisiae, CYC1, is tightly regulated by levels of intracellular heme. Expression is reduced at least 200-fold in cells grown under conditions of heme deficiency. Studies on the regulation of a CYC1-lacZ fused gene and direct determination of mRN...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90309-4
更新日期:1983-04-01 00:00:00
abstract::Membrane scission is essential for intracellular trafficking. While BAR domain proteins such as endophilin have been reported in dynamin-independent scission of tubular membrane necks, the cutting mechanism has yet to be deciphered. Here, we combine a theoretical model, in vitro, and in vivo experiments revealing how ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.05.047
更新日期:2017-06-29 00:00:00
abstract::We have cloned and characterized the rat telomerase protein component 1 gene (TLP1), which is related to the gene for Tetrahymena p80. The cDNA encodes a 2629 amino acid sequence and produces the TLP1 proteins p240 and p230. The anti-TLP1 antibody specifically immunoprecipitated the telomerase activity. Moreover, p240...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81933-9
更新日期:1997-03-21 00:00:00
abstract::The DDB1-CUL4-RBX1 (CRL4) ubiquitin ligase family regulates a diverse set of cellular pathways through dedicated substrate receptors (DCAFs). The DCAF DDB2 detects UV-induced pyrimidine dimers in the genome and facilitates nucleotide excision repair. We provide the molecular basis for DDB2 receptor-mediated cyclobutan...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.10.035
更新日期:2011-11-23 00:00:00
abstract::The exchange of metabolites between the mitochondrial matrix and the cytosol depends on β-barrel channels in the outer membrane and α-helical carrier proteins in the inner membrane. The essential translocase of the inner membrane (TIM) chaperones escort these proteins through the intermembrane space, but the structura...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2018.10.039
更新日期:2018-11-15 00:00:00