Abstract：
:Exchange factors for ARF GTPases (ARF-GEFs) regulate vesicle trafficking in a variety of organisms. The Arabidopsis protein GNOM is a brefeldin A (BFA) sensitive ARF-GEF that is required for the proper polar localization of PIN1, a candidate transporter of the plant hormone auxin. Mutations in GNOM lead to developmental defects that resemble those caused by interfering with auxin transport. Both PIN1 localization and auxin transport are also sensitive to BFA. In this paper, we show that GNOM localizes to endosomes and is required for their structural integrity. We engineered a BFA-resistant version of GNOM. In plants harboring this fully functional GNOM variant, PIN1 localization and auxin transport are no longer sensitive to BFA, while trafficking of other proteins is still affected by the drug. Our results demonstrate that GNOM is required for the recycling of auxin transport components and suggest that ARF-GEFs regulate specific endosomal trafficking pathways.
journal_name
Celljournal_title
Cellauthors
Geldner N,Anders N,Wolters H,Keicher J,Kornberger W,Muller P,Delbarre A,Ueda T,Nakano A,Jürgens Gdoi
10.1016/s0092-8674(03)00003-5subject
Has Abstractpub_date
2003-01-24 00:00:00pages
219-30issue
2eissn
0092-8674issn
1097-4172pii
S0092867403000035journal_volume
112pub_type
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