Abstract：
:A gel electrophoresis DNA binding assay has been used to identify proteins in HeLa cell extracts that specifically bind to the major late promoter of adenovirus. A major late promoter transcription factor MLTF has been detected as a discrete protein-DNA complex. MLTF binds specifically and with high affinity to sequences upstream of the TATA box of the major late promoter. This factor protects a 17 bp (-50 to -66) region in a DNAase I footprinting assay. The same region has been shown to be important for efficient transcription from the major late promoter both in vivo and in vitro. MLTF stimulates in vitro transcription only from a template containing this upstream region. The binding, footprinting, and transcription-stimulatory activities of MLTF cofractionate through two chromatographic steps. These results suggest that direct binding of MLTF to an upstream element activates transcription from the major late promoter.
journal_name
Celljournal_title
Cellauthors
Carthew RW,Chodosh LA,Sharp PAdoi
10.1016/0092-8674(85)90174-6subject
Has Abstractpub_date
1985-12-01 00:00:00pages
439-48issue
2 Pt 1eissn
0092-8674issn
1097-4172pii
0092-8674(85)90174-6journal_volume
43pub_type
杂志文章相关文献
CELL文献大全abstract：:The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.09.053
更新日期：2015-10-22 00:00:00
abstract：:We have determined the 2.0 A crystal structure of a fragment of human fibronectin encompassing the seventh through the RGD-containing tenth type III repeats (FN7-10). The structure reveals an extended rod-like molecule with a long axis of approximately 140 A and highly variable relationships between adjacent domains. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81002-8
更新日期：1996-01-12 00:00:00
abstract：:We have isolated a dominant mutation in a gene called Son of sevenless (Sos) that is an allele-specific suppressor of the sevenless phenotype. This suppressor function is autonomously required in R7 and is sensitive to the dosage of the Sos and bride of sevenless genes. Loss-of-function alleles of Sos are recessive le...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(91)90207-f
更新日期：1991-01-11 00:00:00
abstract：:The protein Par-4 acts in the cytoplasm to trigger cell death signaling via caspase activation and the mitochondrial release of cytochrome c. Burikhanov et al. (2009) now provide surprising evidence that Par-4 can also promote apoptosis from outside the cell, after its secretion in response to endoplasmic reticulum st...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2009.07.007
更新日期：2009-07-23 00:00:00
abstract：:An acute but transient response to insulin is essential for glucose homeostasis in mammals. Chakraborty et al. (2010) uncover a new feedback mechanism regulating insulin signaling. They show that the inositol pyrophosphate IP7, which is produced in response to insulin, inhibits the Akt kinase, a primary effector of in...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.11.040
更新日期：2010-12-10 00:00:00
abstract：:Proteolytic fragments of human plasma fibronectin were used to identify monoclonal antibodies reacting with the various domains of fibronectin. One of these antibodies, which reacts with cell-attachment-promoting fragments of fibronectin, inhibits attachment of cells to fibronectin-coated surfaces. A cell-attachment-p...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(81)90308-1
更新日期：1981-10-01 00:00:00
abstract：:Noncoding RNAs (ncRNA) participate in epigenetic regulation but are poorly understood. Here we characterize the transcriptional landscape of the four human HOX loci at five base pair resolution in 11 anatomic sites and identify 231 HOX ncRNAs that extend known transcribed regions by more than 30 kilobases. HOX ncRNAs ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2007.05.022
更新日期：2007-06-29 00:00:00
abstract：:Using integrative genomic analysis of 360 metastatic castration-resistant prostate cancer (mCRPC) samples, we identified a novel subtype of prostate cancer typified by biallelic loss of CDK12 that is mutually exclusive with tumors driven by DNA repair deficiency, ETS fusions, and SPOP mutations. CDK12 loss is enriched...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2018.04.034
更新日期：2018-06-14 00:00:00
abstract：:Neurons make networks of intercellular connections. Many other cells also send out long cellular extensions and "touch" other cells far away. The extensions are intriguing, but what do they do? Possible rationales for this cell behavior include: (1) forming precise binary connections with the possibility of forming mo...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(03)00156-9
更新日期：2003-03-07 00:00:00
abstract：:Type 2 diabetes (T2D) is a worldwide epidemic with a medical need for additional targeted therapies. Suppression of hepatic glucose production (HGP) effectively ameliorates diabetes and can be exploited for its treatment. We hypothesized that targeting PGC-1α acetylation in the liver, a chemical modification known to ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.03.001
更新日期：2017-03-23 00:00:00
abstract：:Dysfunctional mitochondria accumulate in many human diseases. Accordingly, mitophagy, which removes these mitochondria through lysosomal degradation, is attracting broad attention. Due to uncertainties in the operational principles of conventional mitophagy probes, however, the specificity and quantitativeness of thei...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.04.025
更新日期：2020-05-28 00:00:00
abstract：:Egg laying by the nematode Caenorhabditis elegans requires the functioning of the vulva, the gonad, the egg-laying muscles, and the two HSN neurons, which innervate these muscles. By analyzing a newly isolated mutant (dig-1) that displaces the gonad, we discovered that cell interactions coordinate the spatial relation...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(90)90382-o
更新日期：1990-09-21 00:00:00
abstract：:Suppressor of Hairless (Su(H)) is required at two stages of adult sensory organ development in Drosophila. Complete loss of Su(H) function results in a "neurogenic" phenotype in imaginal discs, in which too many cells adopt the sensory organ precursor cell fate. Su(H) is also involved in controlling the fates of sensi...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90641-o
更新日期：1992-06-26 00:00:00
abstract：:The 2.6 Angstrom crystal structure for human cyclin-dependent kinase 2(CDK2) in complex with CksHs1, a human homolog of essential yeast cell cycle-regulatory proteins suc1 and Cks1, reveals that CksHs1 binds via all four beta strands to the kinase C-terminal lobe. This interface is biologically critical, based upon mu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81065-x
更新日期：1996-03-22 00:00:00
abstract：:Mature HIV-1 particles contain conical-shaped capsids that enclose the viral RNA genome and perform essential functions in the virus life cycle. Previous structural analysis of two- and three-dimensional arrays of the capsid protein (CA) hexamer revealed three interfaces. Here, we present a cryoEM study of a tubular a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.10.010
更新日期：2009-11-13 00:00:00
abstract：:Transcription hinders replication fork progression and stability. The ATR checkpoint and specialized DNA helicases assist DNA synthesis across transcription units to protect genome integrity. Combining genomic and genetic approaches together with the analysis of replication intermediates, we searched for factors coord...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.09.041
更新日期：2012-11-09 00:00:00
abstract：:Proliferating cell nuclear antigen (PCNA) is a cell cycle and growth regulated protein required for replication of SV40 DNA in vitro. Its function was investigated by comparison of the replication products synthesized in its presence or absence. In the completely reconstituted replication system that contains PCNA, DN...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90493-x
更新日期：1988-04-08 00:00:00
abstract：:Assessing the real-world impact of biomedical research is notoriously difficult. Here, we present the framework for building a prospective science-centered information system from scratch that has been afforded by the Sidra Medical and Research Center in Qatar. This experiment is part of the global conversation on max...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.03.004
更新日期：2016-03-24 00:00:00
abstract：:We describe the identification and characterization of the Saccharomyces cerevisiae ZIP2 gene, which encodes a novel meiosis-specific protein essential for synaptonemal complex formation. In the zip2 mutant, chromosomes are homologously paired but not synapsed. The Zip2 protein localizes to discrete foci on meiotic ch...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)81164-2
更新日期：1998-05-01 00:00:00
abstract：:Using positional cloning strategies, we have identified a CTG triplet repeat that undergoes expansion in myotonic dystrophy patients. This sequence is highly variable in the normal population. PCR analysis of the interval containing this repeat indicates that unaffected individuals have been 5 and 27 copies. Myotonic ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(92)90154-5
更新日期：1992-02-21 00:00:00
abstract：:Acquisition of the intestinal microbiota begins at birth, and a stable microbial community develops from a succession of key organisms. Disruption of the microbiota during maturation by low-dose antibiotic exposure can alter host metabolism and adiposity. We now show that low-dose penicillin (LDP), delivered from birt...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.05.052
更新日期：2014-08-14 00:00:00
abstract：:The fasciclin I, II, and III glycoproteins are expressed on different subsets of axon bundles (fascicles) in insect embryos and are thus candidates for surface recognition molecules involved in growth cone guidance. Here we present the sequence of grasshopper fasciclin I and the identification and sequence of the Dros...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(88)90574-0
更新日期：1988-05-20 00:00:00
abstract：:The global financial crisis has hit biotech companies hard both in the US and Europe as venture capital dries up. Finding new sources of long-term financing for translating research into new therapeutics will be essential for maintaining innovation and new drug development by biotech companies. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2009.07.026
更新日期：2009-08-07 00:00:00
abstract：:N(6)-methyladenosine (m(6)A) is the most abundant internal modification of messenger RNA. While the presence of m(6)A on transcripts can impact nuclear RNA fates, a reader of this mark that mediates processing of nuclear transcripts has not been identified. We find that the RNA-binding protein HNRNPA2B1 binds m(6)A-be...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2015.08.011
更新日期：2015-09-10 00:00:00
abstract：:Activation of the cyclin-dependent kinases to promote cell cycle progression requires their association with cyclins as well as phosphorylation of a threonine (residue 161 in human p34cdc2). This phosphorylation is carried out by CAK, the Cdk-activating kinase. We have purified and cloned CAK from S. cerevisiae. Unlik...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80129-4
更新日期：1996-08-23 00:00:00
abstract：:Developmental analysis of a newly isolated maternal effect grandchildless mutant, tudor (tud), in Drosophila melanogaster indicates that tud+ activity is required during oogenesis for the determination and/or formation of primordial germ cells (pole cells) and for normal embryonic abdominal segmentation. Regardless of...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90015-7
更新日期：1985-11-01 00:00:00
abstract：:The MYC oncoproteins are thought to stimulate tumor cell growth and proliferation through amplification of gene transcription, a mechanism that has thwarted most efforts to inhibit MYC function as potential cancer therapy. Using a covalent inhibitor of cyclin-dependent kinase 7 (CDK7) to disrupt the transcription of a...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.10.024
更新日期：2014-11-20 00:00:00
abstract：:The eye lens contains a structural protein (alpha-crystallin), composed of two homologous primary gene products, alpha A2 and alpha B2. In certain rodents, there is another minor alpha-crystallin polypeptide, alpha Ains, which is identical to alpha A2 except for a 22 amino acid insert between residues 63 and 64 of the...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90056-9
更新日期：1983-03-01 00:00:00
abstract：:Cell-cell junctions respond to mechanical forces by changing their organization and function. To gain insight into the mechanochemical basis underlying junction mechanosensitivity, we analyzed tight junction (TJ) formation between the enveloping cell layer (EVL) and the yolk syncytial layer (YSL) in the gastrulating z...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.10.006
更新日期：2019-10-31 00:00:00
abstract：:Phosphodiesterases (PDEs) regulate the local concentration of 3',5' cyclic adenosine monophosphate (cAMP) within cells. cAMP activates the cAMP-dependent protein kinase (PKA). In patients, PDE inhibitors have been linked to heart failure and cardiac arrhythmias, although the mechanisms are not understood. We show that...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2005.07.030
更新日期：2005-10-07 00:00:00
