Coordinated leading and lagging strand synthesis during SV40 DNA replication in vitro requires PCNA.

abstract：:The accurate timing and execution of organelle biogenesis is crucial for cell physiology. Centriole biogenesis is regulated by Polo-like kinase 4 (Plk4) and initiates in S-phase when a daughter centriole grows from the side of a pre-existing mother. Here, we show that a Plk4 oscillation at the base of the growing cent...

journal_title：Cell

authors： Aydogan MG,Steinacker TL,Mofatteh M,Wilmott ZM,Zhou FY,Gartenmann L,Wainman A,Saurya S,Novak ZA,Wong SS,Goriely A,Boemo MA,Raff JW

更新日期：2020-06-25 00:00:00

abstract：:AU-rich elements (AREs), present in mRNA 3'-UTRs, are potent posttranscriptional regulatory signals that can rapidly effect changes in mRNA stability and translation, thereby dramatically altering gene expression with clinical and developmental consequences. In human cell lines, the TNFalpha ARE enhances translation r...

journal_title：Cell

authors： Vasudevan S,Steitz JA

更新日期：2007-03-23 00:00:00

abstract：:Neurons need to alter their response to a given stimulus over time in order for the animal to modify its behavior within a changing environment. Chen et al. now demonstrate that neuronal structure and function are altered coordinately by the history of the cell's activity through an unexpected molecular pathway. ...

journal_title：Cell

authors： Della Santina L,Wong RO

更新日期：2012-09-28 00:00:00

abstract：:Mammalian cells express a spectrum of tubulin isotypes whose relationship to the diversity of microtubule function is unknown. To examine whether different isotypes are segregated into functionally distinct microtubules, we generated immune sera capable of discriminating among the various naturally occurring beta-tubu...

journal_title：Cell

authors： Lewis SA,Gu W,Cowan NJ

更新日期：1987-05-22 00:00:00

abstract：:A major challenge to understanding how cells work together in the central nervous system (CNS) is the heterogeneous cellular composition of the brain. In this issue, Heiman et al. (2008) and Doyle et al. (2008) introduce a new strategy (TRAP) that enables the profiling of translated mRNAs in specific CNS cell populati...

journal_title：Cell

authors： Emery B,Barres BA

更新日期：2008-11-14 00:00:00

abstract：:As axons grow past intermediate targets, they change their responsiveness to guidance cues. Local upregulation of receptor expression is involved, but the mechanisms for this are not clear. Here protein synthesis is traced within individual axons by introducing RNAs encoding visualizable reporters. Individual severed ...

journal_title：Cell

authors： Brittis PA,Lu Q,Flanagan JG

更新日期：2002-07-26 00:00:00

abstract：:A glycoprotein of molecular weight 130,000 (gP130) has been precipitated from the cytoplasm of GR-strain mouse mammary tumor (GR-MMT) cells by a rabbit antiserum (anti-MMTV) to GR-strain mouse mammary tumor virus (GR-MMTV). This protein was not precipitated by antisera specific for detergent-disrupted C3H-strain MMTV ...

journal_title：Cell

authors： Anderson SJ,Naso RB

更新日期：1980-10-01 00:00:00

abstract：:TFIIH is a multisubunit protein complex that plays an essential role in nucleotide excision repair and transcription of protein-coding genes. Here, we report that TFIIH is also required for ribosomal RNA synthesis in vivo and in vitro. In yeast, pre-rRNA synthesis is impaired in TFIIH ts strains. In a mouse, part of c...

journal_title：Cell

authors： Iben S,Tschochner H,Bier M,Hoogstraten D,Hozák P,Egly JM,Grummt I

更新日期：2002-05-03 00:00:00

abstract：:Dysfunctional mitochondria accumulate in many human diseases. Accordingly, mitophagy, which removes these mitochondria through lysosomal degradation, is attracting broad attention. Due to uncertainties in the operational principles of conventional mitophagy probes, however, the specificity and quantitativeness of thei...

journal_title：Cell

authors： Katayama H,Hama H,Nagasawa K,Kurokawa H,Sugiyama M,Ando R,Funata M,Yoshida N,Homma M,Nishimura T,Takahashi M,Ishida Y,Hioki H,Tsujihata Y,Miyawaki A

更新日期：2020-05-28 00:00:00

abstract：:CRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by bacteriophages that inhibit the CRISPR-Cas systems of their host bacteria. These "anti-CRISPRs" were specific to type I CRISPR-Cas systems that d...

journal_title：Cell

authors： Pawluk A,Amrani N,Zhang Y,Garcia B,Hidalgo-Reyes Y,Lee J,Edraki A,Shah M,Sontheimer EJ,Maxwell KL,Davidson AR

更新日期：2016-12-15 00:00:00

abstract：:Synthesis of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a signaling phospholipid, is primarily carried out by phosphatidylinositol 4-phosphate 5-kinase [PI(4)P5K], which has been reported to be regulated by RhoA and Rac1. Unexpectedly, we find that the GTPgammaS-dependent activator of PI(4)P5Kalpha is the smal...

journal_title：Cell

authors： Honda A,Nogami M,Yokozeki T,Yamazaki M,Nakamura H,Watanabe H,Kawamoto K,Nakayama K,Morris AJ,Frohman MA,Kanaho Y

更新日期：1999-11-24 00:00:00

abstract：:We previously demonstrated that pseudogenes complementary to the small nuclear RNAs U1, U2 and U3 are dispersed and abundant in the human genome. Here we report that three pseudogenes (U1.101, U2.13 and U3.5) are flanked by perfect short direct repeats of 16 to 19 base pairs. In all three pseudogenes. the upstream dir...

journal_title：Cell

authors： Van Arsdell SW,Denison RA,Bernstein LB,Weiner AM,Manser T,Gesteland RF

更新日期：1981-10-01 00:00:00

abstract：:Radioactive alpha factor is degraded to discrete biologically inactive fragments by the target a cells of S. cerevisiae, but not by alpha cells which make the pheromone. The pattern of cleavage products and sequence analysis of one fragment indicated that the first scission occurred between leucine 6 and lysine 7. The...

journal_title：Cell

authors： Ciejek E,Thorner J

更新日期：1979-11-01 00:00:00

abstract：:We have generated mice carrying a germline mutation in the tyrosine kinase catalytic domain of the trkB gene. This mutation eliminates expression of gp145trkB, a protein-tyrosine kinase that serves as the signaling receptor for two members of the nerve growth factor family of neurotrophins, brain-derived neurotrophic ...

journal_title：Cell

authors： Klein R,Smeyne RJ,Wurst W,Long LK,Auerbach BA,Joyner AL,Barbacid M

更新日期：1993-10-08 00:00:00

abstract：:DNA repair is an essential process for preserving genome integrity in all organisms. In eukaryotes, recombinational repair is choreographed by multiprotein complexes that are organized into centers (foci). Here, we analyze the cellular response to DNA double-strand breaks (DSBs) and replication stress in Saccharomyces...

journal_title：Cell

authors： Lisby M,Barlow JH,Burgess RC,Rothstein R

更新日期：2004-09-17 00:00:00

abstract：:Drugs selectively targeting CB2 hold promise for treating neurodegenerative disorders, inflammation, and pain while avoiding psychotropic side effects mediated by CB1. The mechanisms underlying CB2 activation and signaling are poorly understood but critical for drug design. Here we report the cryo-EM structure of the ...

journal_title：Cell

authors： Xing C,Zhuang Y,Xu TH,Feng Z,Zhou XE,Chen M,Wang L,Meng X,Xue Y,Wang J,Liu H,McGuire TF,Zhao G,Melcher K,Zhang C,Xu HE,Xie XQ

更新日期：2020-02-20 00:00:00

abstract：:A clonally diverse anticipatory repertoire in which each lymphocyte bears a unique antigen receptor is the central feature of the adaptive immune system that evolved in our vertebrate ancestors. The survival advantage gained through adding this type of adaptive immune system to a pre-existing innate immune system led ...

journal_title：Cell

authors： Cooper MD,Alder MN

更新日期：2006-02-24 00:00:00

abstract：:SOCS1 is an SH2-containing protein that is primarily expressed in thymocytes in a cytokine- and T cell receptor-independent manner. SOCS1 deletion causes perinatal lethality with death by 2-3 weeks. During this period thymic changes include a loss of cellularity and a switch from predominantly CD4+ CD8+ to single posi...

journal_title：Cell

authors： Marine JC,Topham DJ,McKay C,Wang D,Parganas E,Stravopodis D,Yoshimura A,Ihle JN

更新日期：1999-09-03 00:00:00

abstract：:The transition from the juvenile to the adult phase of shoot development in plants is accompanied by changes in vegetative morphology and an increase in reproductive potential. Here, we describe the regulatory mechanism of this transition. We show that miR156 is necessary and sufficient for the expression of the juven...

journal_title：Cell

authors： Wu G,Park MY,Conway SR,Wang JW,Weigel D,Poethig RS

更新日期：2009-08-21 00:00:00

abstract：:Neurons in the cerebral cortex connect through descending pathways to hindbrain and spinal cord to activate muscle and generate movement. Although components of this pathway have been previously generated and studied in vitro, the assembly of this multi-synaptic circuit has not yet been achieved with human cells. Here...

journal_title：Cell

authors： Andersen J,Revah O,Miura Y,Thom N,Amin ND,Kelley KW,Singh M,Chen X,Thete MV,Walczak EM,Vogel H,Fan HC,Paşca SP

更新日期：2020-12-23 00:00:00

abstract：:We have examined the molecular basis of the response of individuals of D. melanogaster to artificial selection for high and low abdominal bristles. By monitoring the fate of particular rDNA spacer length variants associated with individually isolated X and Y chromosomes, we show that flies from the low bristle number ...

journal_title：Cell

authors： Coen ES,Dover GA

更新日期：1983-07-01 00:00:00

abstract：:Cultured preadipose 3T3 cells are induced or enabled to undergo adipose conversion in the presence of an extract of pituitary gland. Adipogenic activity is found in standard growth hormone preparations derived from different species. Further purification of rat growth hormone by several methods does not remove its adi...

journal_title：Cell

authors： Morikawa M,Nixon T,Green H

更新日期：1982-07-01 00:00:00

abstract：:5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 75 muM) inhibited only 60-75% of nuclear heterogeneous RNA (hnRNA) synthesis in HeLa cells, while the appearance of labeled cytoplasmic poly(A)-containing messenger RNA (mRNA) was reduced by approximately 95%. DRB-sensitive and resistant transcripts spanned the e...

journal_title：Cell

authors： Sehgal PB,Darnell JE Jr,Tamm I

更新日期：1976-11-01 00:00:00

abstract：:Different hematopoietic cells produce minute amounts of beta-related interferon (IFN) following induction of differentiation by chemical or natural inducers. The endogenous IFN binds to type I cell surface receptors and modulates gene expression in the producer cells. We show that self-induction of two members of the ...

journal_title：Cell

authors： Resnitzky D,Yarden A,Zipori D,Kimchi A

更新日期：1986-07-04 00:00:00

abstract：:Polycomb group (PcG) and trithorax group (trxG) proteins are critical regulators of numerous developmental genes. To silence or activate gene expression, respectively, PcG and trxG proteins bind to specific regions of DNA and direct the posttranslational modification of histones. Recent work suggests that PcG proteins...

journal_title：Cell

authors： Schuettengruber B,Chourrout D,Vervoort M,Leblanc B,Cavalli G

更新日期：2007-02-23 00:00:00

abstract：:The steroid hormone ecdysone triggers coordinate changes in Drosophila tissue development that result in metamorphosis. To advance our understanding of the genetic regulatory hierarchies controlling this tissue response, we have isolated and characterized a gene, EcR, for a new steroid receptor homolog and have shown ...

journal_title：Cell

authors： Koelle MR,Talbot WS,Segraves WA,Bender MT,Cherbas P,Hogness DS

更新日期：1991-10-04 00:00:00

abstract：:Cells released from primary tumors seed metastases to specific organs by a nonrandom process, implying the involvement of biologically selective mechanisms. Based on clinical, functional, and molecular evidence, we show that the cytokine TGFbeta in the breast tumor microenvironment primes cancer cells for metastasis t...

journal_title：Cell

authors： Padua D,Zhang XH,Wang Q,Nadal C,Gerald WL,Gomis RR,Massagué J

更新日期：2008-04-04 00:00:00

abstract：:We have isolated a population of vesicular carriers involved in the transport (transcytosis) of proteins from the basolateral to the apical plasma membrane of hepatocytes. The obtained fraction was enriched in compartments containing known transcytosed proteins and depleted in elements of the secretory pathway, Golgi ...

journal_title：Cell

authors： Sztul E,Kaplin A,Saucan L,Palade G

更新日期：1991-01-11 00:00:00

abstract：:Scientists are stepping up like never before to support science in the public arena. In big and small ways, scientists are adopting creative ideas to promote science. ...

journal_title：Cell

authors： Matheson S

更新日期：2017-04-06 00:00:00

abstract：:Specification of the germ cell lineage is vital to development and heredity. In mice, the germ cell fate is induced in pluripotent epiblast cells by signaling molecules, yet the underlying mechanism remains unknown. Here we demonstrate that germ cell fate in the epiblast is a direct consequence of Bmp4 signaling from ...

journal_title：Cell

authors： Ohinata Y,Ohta H,Shigeta M,Yamanaka K,Wakayama T,Saitou M

更新日期：2009-05-01 00:00:00