Abstract:
:AU-rich elements (AREs), present in mRNA 3'-UTRs, are potent posttranscriptional regulatory signals that can rapidly effect changes in mRNA stability and translation, thereby dramatically altering gene expression with clinical and developmental consequences. In human cell lines, the TNFalpha ARE enhances translation relative to mRNA levels upon serum starvation, which induces cell-cycle arrest. An in vivo crosslinking-coupled affinity purification method was developed to isolate ARE-associated complexes from activated versus basal translation conditions. We surprisingly found two microRNP-related proteins, fragile-X-mental-retardation-related protein 1 (FXR1) and Argonaute 2 (AGO2), that associate with the ARE exclusively during translation activation. Through tethering and shRNA-knockdown experiments, we provide direct evidence for the translation activation function of both FXR1 and AGO2 and demonstrate their interdependence for upregulation. This novel cell-growth-dependent translation activation role for FXR1 and AGO2 allows new insights into ARE-mediated signaling and connects two important posttranscriptional regulatory systems in an unexpected way.
journal_name
Celljournal_title
Cellauthors
Vasudevan S,Steitz JAdoi
10.1016/j.cell.2007.01.038subject
Has Abstractpub_date
2007-03-23 00:00:00pages
1105-18issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(07)00201-2journal_volume
128pub_type
杂志文章相关文献
CELL文献大全abstract::Cultured NIH 3T3 cells devoid of endogenous EGF receptors were transfected with cDNA constructs encoding either the human EGF receptor or an EGF receptor mutant in which Lys721, a key residue in the ATP binding site, was replaced with an alanine residue. The mutant receptor was properly processed, and it displayed bot...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90147-4
更新日期:1987-10-23 00:00:00
abstract::We have employed repeat units of herpes simplex virus (HSV) defective genomes to derive a cloning-amplifying vector (amplicon) that can replicate in eucaryotic cells in the presence of standard HSV helper virus. The design of the HSV amplicon system is based on the previous observation that cotransfection of cells wit...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90035-6
更新日期:1982-08-01 00:00:00
abstract::Activating mutations of FLT3 occur in about 30% of acute myeloid leukemia (AML) cases and are associated with relapse and poor prognosis. Midostaurin is the first drug approved for AML since 2000, and the first multi-kinase inhibitor approved for the FLT3-mutant subtype. To view this Bench to Bedside, open or download...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.11.007
更新日期:2017-11-16 00:00:00
abstract::The Wee1 kinase phosphorylates and inhibits cyclin-dependent kinase 1 (Cdk1), thereby delaying entry into mitosis until appropriate conditions have been met. An understanding of the mechanisms that regulate Wee1 should provide new insight into how cells make the decision to enter mitosis. We report here that Swe1, the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.05.029
更新日期:2005-08-12 00:00:00
abstract::The distribution of sense and antisense strand DNA mutations on transcribed duplex DNA contributes to the development of immune and neural systems along with the progression of cancer. Because developmentally matured B cells undergo biologically programmed strand-specific DNA mutagenesis at focal DNA/RNA hybrid struct...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.03.043
更新日期:2017-04-20 00:00:00
abstract::During yeast cell division, aggregates of damaged proteins are segregated asymmetrically between the bud and the mother. It is thought that protein aggregates are cleared from the bud via actin cable-based retrograde transport toward the mother and that Bni1p formin regulates this transport. Here, we examined the dyna...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2011.11.002
更新日期:2011-11-23 00:00:00
abstract::Generation of potent antibodies by a mutation-selection process called affinity maturation is a key component of effective immune responses. Antibodies that protect against highly mutable pathogens must neutralize diverse strains. Developing effective immunization strategies to drive their evolution requires understan...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.01.027
更新日期:2015-02-12 00:00:00
abstract::Enhancers provide critical information directing cell-type-specific transcriptional programs, regulated by binding of signal-dependent transcription factors and their associated cofactors. Here, we report that the most strongly activated estrogen (E2)-responsive enhancers are characterized by trans-recruitment and in ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.08.027
更新日期:2014-10-09 00:00:00
abstract::Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C∗ complex) at an average resolution of 3.76 Å. The splicing factor Prp17...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.04.033
更新日期:2017-05-18 00:00:00
abstract::A comprehensive analysis uncovered a set of yeast proteins promoting protein-based inheritance that shares many of the non-Mendelian properties of prions. Lacking any sequence or structural signatures of known prions, these proteins represent a new class of non-amyloid, protein-based epigenetic determinants that can c...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2016.09.036
更新日期:2016-10-06 00:00:00
abstract::We report genome-wide DNA data for 73 individuals from five archaeological sites across the Bronze and Iron Ages Southern Levant. These individuals, who share the "Canaanite" material culture, can be modeled as descending from two sources: (1) earlier local Neolithic populations and (2) populations related to the Chal...
journal_title:Cell
pub_type: 历史文章,杂志文章
doi:10.1016/j.cell.2020.04.024
更新日期:2020-05-28 00:00:00
abstract::A safe and controlled manipulation of endocytosis in vivo may have disruptive therapeutic potential. Here, we demonstrate that the anti-emetic/anti-psychotic prochlorperazine can be repurposed to reversibly inhibit the in vivo endocytosis of membrane proteins targeted by therapeutic monoclonal antibodies, as directly ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.02.019
更新日期:2020-03-05 00:00:00
abstract::The germinal center (GC) reaction produces high-affinity antibodies by random mutation and selective clonal expansion of B cells with high-affinity receptors. The mechanism by which B cells are selected remains unclear, as does the role of the two anatomically defined areas of the GC, light zone (LZ) and dark zone (DZ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2010.10.032
更新日期:2010-11-12 00:00:00
abstract::Academic travel has a substantial carbon footprint. The ongoing pandemic has propelled the development and adoption of technologies for online delivery of seminars and remote attendance at scientific conferences. This should not lead to the complete elimination of in-person events, but the scientific community must se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.07.026
更新日期:2020-08-20 00:00:00
abstract::Transduction of cellular genes by retroviruses requires two recombinations: one to form the left-hand junction between cellular gene and viral genome, the other to form the right-hand junction. Previous findings raised the possibility that the right-hand recombination might use RNA molecules as intermediates. We now p...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(86)90016-4
更新日期:1986-03-28 00:00:00
abstract::We employed gene targeting to study H2AX, a histone variant phosphorylated in chromatin surrounding DNA double-strand breaks. Mice deficient for both H2AX and p53 (H(delta/delta)P(-/-)) rapidly developed immature T and B lymphomas and solid tumors. Moreover, H2AX haploinsufficiency caused genomic instability in normal...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00566-x
更新日期:2003-08-08 00:00:00
abstract::The liver is capable of completely regenerating itself in response to injury and after partial hepatectomy. In liver of old animals, the proliferative response is dramatically reduced, the mechanism for which is unknown. The liver specific protein, C/EBPalpha, normally arrests proliferation of hepatocytes through inhi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(03)00318-0
更新日期:2003-05-16 00:00:00
abstract::gamma-Irradiation of human diploid fibroblasts in the G1 interval caused arrest of the cell cycle prior to S phase. This cell cycle block was correlated with a lack of activation of both cyclin E-Cyclin-dependent kinase 2 (Cdk2) and cyclin A-Cdk2 kinases and depended on wild-type p53. Although the accumulation of cycl...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90379-4
更新日期:1994-03-25 00:00:00
abstract::MicroRNAs interact with Argonaute proteins to guide posttranscriptional gene silencing. Eiring et al. (2010) now show that miR-328 has a second function, acting as a decoy by binding to hnRNP E2 and lifting its translational repression of an mRNA involved in myeloid cell differentiation. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2010.02.020
更新日期:2010-03-05 00:00:00
abstract::The Polycomb group (Pc-G) proteins are responsible for keeping developmental regulators, like homeotic genes, stably and inheritably repressed during Drosophila development. Several similarities to a protein class involved in heterochromatin formation suggest that the Pc-G exerts its function at the higher order chrom...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90328-n
更新日期:1993-12-17 00:00:00
abstract::Malignancy can be suppressed by the immune system in a process termed immunosurveillance. However, to what extent immunosurveillance occurs in spontaneous cancers and the composition of participating cell types remains obscure. Here, we show that cell transformation triggers a tissue-resident lymphocyte response in on...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.01.002
更新日期:2016-01-28 00:00:00
abstract::Five single base pair mutations that increase expression of the tnpA (transposase) gene of the Tn3 transposon approximately 30-fold, but which still allow the gene to be regulated, have been isolated by using a generally applicable procedure that involves distally linked lac gene fusions. The mutations, which are all ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(82)90352-x
更新日期:1982-02-01 00:00:00
abstract::Radioactive alpha factor is degraded to discrete biologically inactive fragments by the target a cells of S. cerevisiae, but not by alpha cells which make the pheromone. The pattern of cleavage products and sequence analysis of one fragment indicated that the first scission occurred between leucine 6 and lysine 7. The...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90117-x
更新日期:1979-11-01 00:00:00
abstract::We have isolated three alleles of a novel Drosophila clock gene, double-time (dbt). Short- (dbtS) and long-period (dbtL) mutants alter both behavioral rhythmicity and molecular oscillations from previously identified clock genes, period and timeless. A third allele, dbtP, causes pupal lethality and eliminates circadia...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81224-6
更新日期:1998-07-10 00:00:00
abstract::Planar polarity is a global, tissue-level phenomenon that coordinates cell behavior in a two-dimensional plane. The Frizzled/planar cell polarity (PCP) and anterior-posterior (AP) patterning systems for planar polarity operate in a variety of cell types and provide direction to cells with different morphologies and be...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2007.05.050
更新日期:2007-06-15 00:00:00
abstract::Dysfunctional mitochondria accumulate in many human diseases. Accordingly, mitophagy, which removes these mitochondria through lysosomal degradation, is attracting broad attention. Due to uncertainties in the operational principles of conventional mitophagy probes, however, the specificity and quantitativeness of thei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.04.025
更新日期:2020-05-28 00:00:00
abstract::Biological membranes undergo constant remodeling by membrane fission and fusion to change their shape and to exchange material between subcellular compartments. During clathrin-mediated endocytosis, the dynamic assembly and disassembly of protein scaffolds comprising members of the bin-amphiphysin-rvs (BAR) domain pro...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2014.02.017
更新日期:2014-02-27 00:00:00
abstract::Cytoplasmic dynein is a motor protein that exerts force on microtubules. To generate force for the movement of large organelles, dynein needs to be anchored, with the anchoring sites being typically located at the cell cortex. However, the mechanism by which dyneins target sites where they can generate large collectiv...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.05.020
更新日期:2013-06-20 00:00:00
abstract::Retroviral DNA integration involves a coordinated set of DNA cutting and joining reactions. We find that the IN protein of Moloney murine leukemia virus (MoMLV) is the only viral protein required to accomplish these reactions in vitro. IN protein has a site-specific nuclease activity that cleaves 2 nucleotides from th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(90)90126-y
更新日期:1990-08-24 00:00:00
abstract::The human geneticist Archibald Garrod noted in 1931 that, "It is, of necessity, no easy matter to distinguish between immunity which is inborn and that which has been acquired" (The Inborn Factors in Disease). In this issue of Cell, Brodin et al. show that the heritability of blood counts rapidly decreases with age fo...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2014.12.029
更新日期:2015-01-15 00:00:00