Abstract:
:We employed gene targeting to study H2AX, a histone variant phosphorylated in chromatin surrounding DNA double-strand breaks. Mice deficient for both H2AX and p53 (H(delta/delta)P(-/-)) rapidly developed immature T and B lymphomas and solid tumors. Moreover, H2AX haploinsufficiency caused genomic instability in normal cells and, on a p53-deficient background, early onset of various tumors including more mature B lymphomas. Most H2AX(delta/delta)p53(-/-) or H2AX(+/delta)p53(-/-) B lineage lymphomas harbored chromosome 12 (IgH)/15 (c-myc) translocations with hallmarks of either aberrant V(D)J or class switch recombination. In contrast, H2AX(delta/delta)p53(-/-) thymic lymphomas had clonal translocations that did not involve antigen receptor loci and which likely occurred during cellular expansion. Thus, H2AX helps prevent aberrant repair of both programmed and general DNA breakage and, thereby, functions as a dosage-dependent suppressor of genomic instability and tumors in mice. Notably, H2AX maps to a cytogenetic region frequently altered in human cancers, possibly implicating similar functions in man.
journal_name
Celljournal_title
Cellauthors
Bassing CH,Suh H,Ferguson DO,Chua KF,Manis J,Eckersdorff M,Gleason M,Bronson R,Lee C,Alt FWdoi
10.1016/s0092-8674(03)00566-xsubject
Has Abstractpub_date
2003-08-08 00:00:00pages
359-70issue
3eissn
0092-8674issn
1097-4172pii
S009286740300566Xjournal_volume
114pub_type
杂志文章相关文献
CELL文献大全abstract::Angiogenesis is an important event for embryonic organogenesis as well as for tissue repair in the adult. Here, we show that hematopoietic stem cells (HSCs) play important roles for angiogenesis during embryogenesis. To investigate the role of HSCs in endothelial cell (EC) development, we analyzed AML1-deficient embry...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00025-8
更新日期:2000-07-21 00:00:00
abstract::Septate and tight junctions are thought to seal neighboring cells together and to function as barriers between epithelial cells. We have characterized a novel member of the neurexin family, Neurexin IV (NRX), which is localized to septate junctions (SJs) of epithelial and glial cells. NRX is a transmembrane protein wi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81800-0
更新日期:1996-12-13 00:00:00
abstract::How and when memory T cells form during an immune response are long-standing questions. To better understand memory CD8 T cell development, a time course of gene expression and functional changes in antigen-specific T cells during viral infection was evaluated. The expression of many genes continued to change after vi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(02)01139-x
更新日期:2002-12-13 00:00:00
abstract::In Saccharomyces cerevisiae, mothers and daughters have distinct fates. We show that Cbk1 kinase and its interacting protein Mob2 regulate this asymmetry by inducing daughter-specific genetic programs. Daughter-specific expression is due to Cbk1/Mob2-dependent activation and localization of the Ace2 transcription fact...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00596-7
更新日期:2001-12-14 00:00:00
abstract::The transcriptional induction of heat shock genes is mediated by the heat shock transcription factor (HSF). We have investigated the oligomerization state of HSF from S. cerevisiae and find that it forms a trimer in solution and when bound to DNA. Trimerization is mediated by a region of HSF that, like the leucine zip...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(89)90604-1
更新日期:1989-12-01 00:00:00
abstract::Hair cells are mechanosensors for the perception of sound, acceleration, and fluid motion. Mechanotransduction channels in hair cells are gated by tip links, which connect the stereocilia of a hair cell in the direction of their mechanical sensitivity. The molecular constituents of the mechanotransduction channels of ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.10.041
更新日期:2012-12-07 00:00:00
abstract::Much like the "microbe hypothesis" put forth over 150 years ago, the "prion hypothesis" can be definitely proven only if a prion disease is engendered in a natural host from an infectious prion produced in vitro. In this issue of Cell, come very close to accomplishing this goal by producing a prion disease in a natura...
journal_title:Cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.cell.2005.04.002
更新日期:2005-04-22 00:00:00
abstract::The rps12 gene codes for chloroplast ribosomal protein S12. In the tobacco chloroplast genome (156 kbp circular DNA), exons II and III of this gene are separated by an intron of 536 bases and are present in two copies in the inverted repeat region, while exon I is located in the large single copy region at a distance ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90361-8
更新日期:1987-01-16 00:00:00
abstract::Telomeres are thought to be maintained by the preferential recruitment of telomerase to the shortest telomeres. The extension of the G-rich telomeric strand by telomerase is also believed to be coordinated with the complementary synthesis of the C strand by the conventional replication machinery. However, we show that...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.05.026
更新日期:2009-08-07 00:00:00
abstract::Axonal transport has been intensively examined as a good model for studying the mechanism of organelle transport in cells, but it is still unclear how different types of membrane organelles are transported through the nerve axon. To elucidate the function of this mechanism, we have cloned KIF1A, a novel neuron-specifi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(95)90538-3
更新日期:1995-06-02 00:00:00
abstract::Rodent cells resistant to PALA, a specific inhibitor of the aspartate transcarbamylase activity of the multifunctional CAD protein, overproduce CAD as a result of amplification of the CAD gene. We cloned a functional CAD gene from Syrian hamster cells using a cosmid vector. Two independently isolated cosmids containin...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(81)90410-4
更新日期:1981-12-01 00:00:00
abstract::To reconstruct modern human evolutionary history and identify loci that have shaped hunter-gatherer adaptation, we sequenced the whole genomes of five individuals in each of three different hunter-gatherer populations at > 60× coverage: Pygmies from Cameroon and Khoesan-speaking Hadza and Sandawe from Tanzania. We ide...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.07.009
更新日期:2012-08-03 00:00:00
abstract::Suppressor of Hairless (Su(H)) is required at two stages of adult sensory organ development in Drosophila. Complete loss of Su(H) function results in a "neurogenic" phenotype in imaginal discs, in which too many cells adopt the sensory organ precursor cell fate. Su(H) is also involved in controlling the fates of sensi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90641-o
更新日期:1992-06-26 00:00:00
abstract::Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted P...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2017.05.028
更新日期:2017-06-01 00:00:00
abstract::Nociceptin and its receptor are widely distributed throughout the brain in regions associated with reward behavior, yet how and when they act is unknown. Here, we dissected the role of a nociceptin peptide circuit in reward seeking. We generated a prepronociceptin (Pnoc)-Cre mouse line that revealed a unique subpopula...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.06.034
更新日期:2019-07-25 00:00:00
abstract::Neurons in the cerebral cortex connect through descending pathways to hindbrain and spinal cord to activate muscle and generate movement. Although components of this pathway have been previously generated and studied in vitro, the assembly of this multi-synaptic circuit has not yet been achieved with human cells. Here...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.11.017
更新日期:2020-12-23 00:00:00
abstract::The structure of chicken erythrocyte and chicken liver chromatin has been studied by enzymatic digestion with micrococcal nuclease and DNAase I. Comparison of the fragments produced by brief digestion with micrococcal nuclease indicates that chicken erythrocyte chromatin has a longer nucleosome repeat than chicken liv...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90045-3
更新日期:1976-12-01 00:00:00
abstract::Multisite hyperphosphorylation of tau has been implicated in the pathogenesis of neurodegenerative diseases including Alzheimer's disease (AD). However, the phosphorylation events critical for tau toxicity and mechanisms regulating these events are largely unknown. Here we show that Drosophila PAR-1 kinase initiates t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(04)00170-9
更新日期:2004-03-05 00:00:00
abstract::Nuclear receptors undergo ligand-dependent conformational changes that are required for corepressor-coactivator exchange, but whether there is an actual requirement for specific epigenetic landmarks to impose ligand dependency for gene activation remains unknown. Here we report an unexpected and general strategy that ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2006.12.038
更新日期:2007-02-09 00:00:00
abstract::The rapid cell proliferation characteristic of early animal embryos is accomplished with an abbreviated cell cycle and no DNA replication checkpoint. Blythe and Wieschaus provide evidence that nascent zygotic transcription precedes—and may trigger—this checkpoint at the midblastula transition. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2015.02.051
更新日期:2015-03-12 00:00:00
abstract::Goosecoid (gsc), a homeobox gene expressed specifically in the dorsal blastopore lip of the Xenopus gastrula, is considered to play an important role in Spemann's organizer phenomenon. Lineage tracing and time-lapse microscopy were used to follow the fate of embryonic cells microinjected with gsc mRNA. Microinjected g...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(93)90069-3
更新日期:1993-02-26 00:00:00
abstract::In Drosophila, the fat body (FB), a functional analog of the vertebrate adipose tissue, is the nutrient sensor that conveys the nutrient status to the insulin-producing cells (IPCs) in the fly brain to release Drosophila insulin-like peptides (Dilps). Dilp secretion in turn regulates energy balance and promotes system...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2012.08.019
更新日期:2012-09-28 00:00:00
abstract::Mycobacteria are unusual in encoding two GroEL paralogs, GroEL1 and GroEL2. GroEL2 is essential--presumably providing the housekeeping chaperone functions--while groEL1 is nonessential, contains the attB site for phage Bxb1 integration, and encodes a putative chaperone with unusual structural features. Inactivation of...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.09.012
更新日期:2005-12-02 00:00:00
abstract::Sucrose gradient analysis of DNA isolated from detergent-pronase lysates of adult flies has been used to look for ribosomal genes not integrated into the DNA of the chromosome in genotypes containing various combinations of inversions having breakpoints in the proximal heterochromatin of the X chromosome. Unintegrated...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(77)90056-3
更新日期:1977-06-01 00:00:00
abstract::Salmonella possesses several flagella, each capable of counterclockwise and clockwise rotation. Counterclockwise rotation produces swimming, clockwise rotation produces tumbling. Switching between senses occurs stochastically. The rotational sense of individual flagella on a single cell could be monitored under specia...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90501-9
更新日期:1983-01-01 00:00:00
abstract::Immune homeostasis in the intestine involves complex interactions between microbial organisms and host cells. In this issue, Garrett et al. (2007) describe a communicable form of colitis that is induced by deficiency of the transcription factor T-bet in cells of the innate immune system. ...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2007.09.030
更新日期:2007-10-05 00:00:00
abstract::beta-glucocerebrosidase, the enzyme defective in Gaucher disease, is targeted to the lysosome independently of the mannose-6-phosphate receptor. Affinity-chromatography experiments revealed that the lysosomal integral membrane protein LIMP-2 is a specific binding partner of beta-glucocerebrosidase. This interaction in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.10.018
更新日期:2007-11-16 00:00:00
abstract::The science advisor to the US president has the opportunity to influence how scientific research is supported by the federal government, as well as the ways in which science and technology can be applied to meet the needs of society. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.11.024
更新日期:2009-11-25 00:00:00
abstract::We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90016-3
更新日期:1979-02-01 00:00:00
abstract::We have isolated spontaneous temperature-resistant revertants of a temperature-sensitive mutation (rpoD800) in the sigma subunit of E. coli K12 RNA polymerase. These revertants still contained the rpoD800 allele. They were mucoid, and sensitive to ultraviolet light and the radiomimetic agent nitrofurantoin, which are ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(83)90505-6
更新日期:1983-01-01 00:00:00