p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest.


:gamma-Irradiation of human diploid fibroblasts in the G1 interval caused arrest of the cell cycle prior to S phase. This cell cycle block was correlated with a lack of activation of both cyclin E-Cyclin-dependent kinase 2 (Cdk2) and cyclin A-Cdk2 kinases and depended on wild-type p53. Although the accumulation of cyclin A was strongly inhibited in gamma-irradiated cells, cyclin E accumulated and bound Cdk2 at normal levels but remained in an inactive state. We found that both whole-cell lysates and inactive cyclin E-Cdk2 complexes prepared from irradiated cells contained an activity capable of inactivating cyclin E-Cdk2 complexes. The protein responsible for this activity was shown to be p21CIP1/WAF1, recently described as a p53-inducible Cdk inhibitor. Our data suggest a model in which ionizing radiation confers G1 arrest via the p53-mediated induction of a Cdk inhibitor protein.






Dulić V,Kaufmann WK,Wilson SJ,Tlsty TD,Lees E,Harper JW,Elledge SJ,Reed SI




Has Abstract


1994-03-25 00:00:00
















  • p53 opens the mitochondrial permeability transition pore to trigger necrosis.

    abstract::Ischemia-associated oxidative damage leading to necrosis is a major cause of catastrophic tissue loss, and elucidating its signaling mechanism is therefore of paramount importance. p53 is a central stress sensor responding to multiple insults, including oxidative stress to orchestrate apoptotic and autophagic cell dea...


    pub_type: 杂志文章


    authors: Vaseva AV,Marchenko ND,Ji K,Tsirka SE,Holzmann S,Moll UM

    更新日期:2012-06-22 00:00:00

  • A symmetrical six-base-pair target site sequence determines Tn10 insertion specificity.

    abstract::Transposon Tn10 inserts at many sites in the bacterial chromosome, but preferentially inserts at particular hotspots. We believe we have identified the target DNA signal responsible for this specificity. We have determined the DNA sequences of 11 Tn10 insertion sites and identified a particular 6 base pair (bp) symmet...


    pub_type: 杂志文章


    authors: Halling SM,Kleckner N

    更新日期:1982-01-01 00:00:00

  • Avant garde fatty acid synthesis by trypanosomes.

    abstract::In this issue of Cell, Lee et al. (2006) report that the parasite Trypanosoma brucei synthesizes fatty acids in an unconventional way. T. brucei and two other trypanosomes use enzymes called elongases to synthesize myristate, a fourteen carbon (C14) fatty acid essential for pathogenesis. This is an unexpected finding ...


    pub_type: 评论,杂志文章


    authors: Cronan JE

    更新日期:2006-08-25 00:00:00

  • Postnatal development of a demyelinating disease in avian spinal cord chimeras.

    abstract::Xenogeneic spinal cord chimeras were constructed by grafting fragments of quail neural primordium into chick embryos at 2 days of incubation. Hatched birds displayed normal motor behavior for about 5 to 7 weeks, whereupon they developed a neurological syndrome; in the grafted spinal cord the pathological signs of the ...


    pub_type: 杂志文章


    authors: Kinutani M,Coltey M,Le Douarin NM

    更新日期:1986-04-25 00:00:00

  • The NAD(+)/Sirtuin Pathway Modulates Longevity through Activation of Mitochondrial UPR and FOXO Signaling.

    abstract::NAD(+) is an important cofactor regulating metabolic homeostasis and a rate-limiting substrate for sirtuin deacylases. We show that NAD(+) levels are reduced in aged mice and Caenorhabditis elegans and that decreasing NAD(+) levels results in a further reduction in worm lifespan. Conversely, genetic or pharmacological...


    pub_type: 杂志文章


    authors: Mouchiroud L,Houtkooper RH,Moullan N,Katsyuba E,Ryu D,Cantó C,Mottis A,Jo YS,Viswanathan M,Schoonjans K,Guarente L,Auwerx J

    更新日期:2013-07-18 00:00:00

  • The cellular mechanisms of neuronal swelling underlying cytotoxic edema.

    abstract::Cytotoxic brain edema triggered by neuronal swelling is the chief cause of mortality following brain trauma and cerebral infarct. Using fluorescence lifetime imaging to analyze contributions of intracellular ionic changes in brain slices, we find that intense Na(+) entry triggers a secondary increase in intracellular ...


    pub_type: 杂志文章


    authors: Rungta RL,Choi HB,Tyson JR,Malik A,Dissing-Olesen L,Lin PJC,Cain SM,Cullis PR,Snutch TP,MacVicar BA

    更新日期:2015-04-23 00:00:00

  • Adrenergic Signaling in Muscularis Macrophages Limits Infection-Induced Neuronal Loss.

    abstract::Enteric-associated neurons (EANs) are closely associated with immune cells and continuously monitor and modulate homeostatic intestinal functions, including motility and nutrient sensing. Bidirectional interactions between neuronal and immune cells are altered during disease processes such as neurodegeneration or irri...


    pub_type: 杂志文章


    authors: Matheis F,Muller PA,Graves CL,Gabanyi I,Kerner ZJ,Costa-Borges D,Ahrends T,Rosenstiel P,Mucida D

    更新日期:2020-01-09 00:00:00

  • Transfer of murine intracisternal A particle phenotype in chloramphenicol-resistant cytoplasts.

    abstract::Murine intracisternal A particles have a number of properties which are common to known RNA tumor viruses, but horizontal transmission has not been previously demonstrated. The apparent absence of infectivity may be related to the failure of these particles to be released from cisternae of endo-plasmic reticulum. Prev...


    pub_type: 杂志文章


    authors: Malech HL,Wivel NA

    更新日期:1976-11-01 00:00:00

  • Sensory Neurons that Detect Stretch and Nutrients in the Digestive System.

    abstract::Neural inputs from internal organs are essential for normal autonomic function. The vagus nerve is a key body-brain connection that monitors the digestive, cardiovascular, and respiratory systems. Within the gastrointestinal tract, vagal sensory neurons detect gut hormones and organ distension. Here, we investigate th...


    pub_type: 杂志文章


    authors: Williams EK,Chang RB,Strochlic DE,Umans BD,Lowell BB,Liberles SD

    更新日期:2016-06-30 00:00:00

  • Compete globally, bud locally.

    abstract::How cells generate a single axis of polarity for mating, division, and movement is unknown. In this issue, Howell et al. (2009) use a synthetic biology approach to demonstrate that rapid competition for a soluble signaling component (Bem1) is essential to ensure a unique axis of polarity in budding yeast. ...


    pub_type: 评论,杂志文章


    authors: Houk AR,Millius A,Weiner OD

    更新日期:2009-11-13 00:00:00

  • Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion.

    abstract::We describe genetic and physical characterization of rearrangements of chromosome III which result in changes of cell type in S. cerevisiae. Two types of rearrangements were obtained as rare events which caused a change at the locus controlling cell type, MAT, associated with a recessive lethal mutation, in one case f...


    pub_type: 杂志文章


    authors: Strathern JN,Newlon CS,Herskowitz I,Hicks JB

    更新日期:1979-10-01 00:00:00

  • trans-repression of the mouse c-fos promoter: a novel mechanism of Fos-mediated trans-regulation.

    abstract::Fos protein can trans-activate AP-1-dependent gene expression and trans-repress the c-fos promoter. Although we find that trans-repression is enhanced by coexpression of c-Jun, it does not require any of the AP-1 or ATF sites in the mouse c-fos promoter. A major target for repression is the serum response element (SRE...


    pub_type: 杂志文章


    authors: Lucibello FC,Lowag C,Neuberg M,Müller R

    更新日期:1989-12-22 00:00:00

  • RORalpha-mediated Purkinje cell development determines disease severity in adult SCA1 mice.

    abstract::Spinocerebellar ataxia type 1 (SCA1) is one of nine inherited, typically adult onset, polyglutamine neurodegenerative diseases. To examine whether development impacts SCA1, we used a conditional transgenic mouse model of SCA1 to delay the postnatal expression of mutant ATXN1 until after completion of cerebellar develo...


    pub_type: 杂志文章


    authors: Serra HG,Duvick L,Zu T,Carlson K,Stevens S,Jorgensen N,Lysholm A,Burright E,Zoghbi HY,Clark HB,Andresen JM,Orr HT

    更新日期:2006-11-17 00:00:00

  • Novel inheritance of the murine severe combined anemia and thrombocytopenia (Scat) phenotype.

    abstract::The phenotype of the autosomal recessive mutation scat includes severe intermittent bleeding, depletion of platelets, and circulating anti-platelet antibodies. In this study, we have mapped the scat mutation to mouse chromosome 8 and shown that the immune component is a secondary consequence of the gene defect. Surpri...


    pub_type: 杂志文章


    authors: Peters LL,Barker JE

    更新日期:1993-07-16 00:00:00

  • A movable and regulable inactivation function within the steroid binding domain of the glucocorticoid receptor.

    abstract::The glucocorticoid receptor is a signal transducer that interacts both with the signal and with the genes it regulates. We showed previously that nuclear localization of the receptor requires hormone binding. We have now constructed recombinant receptors that relieve hormonal control of nuclear localization, and we de...


    pub_type: 杂志文章


    authors: Picard D,Salser SJ,Yamamoto KR

    更新日期:1988-09-23 00:00:00

  • Generation of unexpected editing patterns in Leishmania tarentolae mitochondrial mRNAs: misediting produced by misguiding.

    abstract::We have analyzed the generation of unexpected patterns of RNA editing, i.e., those not following a strict 3' to 5' progression, which occur in junction regions between fully edited and preedited sequences. Evidence is presented that these patterns are generated by misediting due to specific events of misguiding. Mised...


    pub_type: 杂志文章


    authors: Sturm NR,Maslov DA,Blum B,Simpson L

    更新日期:1992-08-07 00:00:00

  • A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes.

    abstract::The thymus exports a selected subset of virgin T lymphocytes to the peripheral lymphoid organs. The mature phenotype of these thymus emigrants is similar to that of medullary thymocytes and has been cited as supporting a medullary rather than cortical exit site. Using the monoclonal antibody MEL-14, we identify a 1%-3...


    pub_type: 杂志文章


    authors: Reichert RA,Gallatin WM,Butcher EC,Weissman IL

    更新日期:1984-08-01 00:00:00

  • Divided within: heterogeneity within adult stem cell pools.

    abstract::In this issue, Wilson et al. (2008) demonstrate that there are two functional subsets of hematopoietic stem cells that have distinctive kinetics of cell cycling. They present evidence that cells may transition between the two kinetic states, establishing one subpopulation that is ready to proliferate and another that ...


    pub_type: 评论,杂志文章


    authors: Raaijmakers MH,Scadden DT

    更新日期:2008-12-12 00:00:00

  • Procollagen and collagen produced by a teratocarcinoma-derived cell line, TSD4: evidence for a new molecular form of collagen.

    abstract::Procollagen and collagen were isolated from the culture medium and cell layer of line TSD4 (obtained from mouse teratocarcinoma OTT6050). SDS-polyacrylamide gel electrophoresis of the highly purified procollagen fraction demonstrated that the fraction is composed of theta chains (150,000 daltons), pro alpha chains (13...


    pub_type: 杂志文章


    authors: Little CD,Church RL,Miller RA,Ruddle FH

    更新日期:1977-02-01 00:00:00

  • Efficient direct reprogramming of mature amniotic cells into endothelial cells by ETS factors and TGFβ suppression.

    abstract::ETS transcription factors ETV2, FLI1, and ERG1 specify pluripotent stem cells into induced vascular endothelial cells (iVECs). However, iVECs are unstable and drift toward nonvascular cells. We show that human midgestation c-Kit(-) lineage-committed amniotic cells (ACs) can be reprogrammed into vascular endothelial ce...


    pub_type: 杂志文章


    authors: Ginsberg M,James D,Ding BS,Nolan D,Geng F,Butler JM,Schachterle W,Pulijaal VR,Mathew S,Chasen ST,Xiang J,Rosenwaks Z,Shido K,Elemento O,Rabbany SY,Rafii S

    更新日期:2012-10-26 00:00:00

  • Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4+ T Cells in COVID-19.

    abstract::The contribution of CD4+ T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present single-cell transcriptomic analysis of >100,000 viral antigen-reactive CD4+ T cells from 40 COVID-19 patients. In hospitalized patients compared to non-hospitalized patients, we found...


    pub_type: 杂志文章


    authors: Meckiff BJ,Ramírez-Suástegui C,Fajardo V,Chee SJ,Kusnadi A,Simon H,Eschweiler S,Grifoni A,Pelosi E,Weiskopf D,Sette A,Ay F,Seumois G,Ottensmeier CH,Vijayanand P

    更新日期:2020-11-25 00:00:00

  • A posttermination ribosomal complex is the guanine nucleotide exchange factor for peptide release factor RF3.

    abstract::The mechanism by which peptide release factor RF3 recycles RF1 and RF2 has been clarified and incorporated in a complete scheme for translation termination. Free RF3 is in vivo stably bound to GDP, and ribosomes in complex with RF1 or RF2 act as guanine nucleotide exchange factors (GEF). Hydrolysis of peptidyl-tRNA by...


    pub_type: 杂志文章


    authors: Zavialov AV,Buckingham RH,Ehrenberg M

    更新日期:2001-10-05 00:00:00

  • Microheterogeneity among carbohydrate structures at the cell surface may be important in recognition phenomena.

    abstract::Independently derived mutants of Chinese hamster ovary cells have been isolated and shown to exhibit a subtle glycosylation defect resulting in the premature termination of certain asparagine-linked carbohydrate moieties. This carbohydrate alteration is akin to the types of structural variation termed microheterogenei...


    pub_type: 杂志文章


    authors: Stanley P,Sudo T

    更新日期:1981-03-01 00:00:00

  • A small region on the X chromosome of Drosophila regulates a key gene that controls sex determination and dosage compensation.

    abstract::In Drosophila, flies with two X chromosomes are females, with one X chromosome, males. We investigated the presence of sex determining factors on the X chromosome by constructing genotypes with one X and various X-chromosomal duplications. We found that female determining factors are not evenly distributed along the X...


    pub_type: 杂志文章


    authors: Steinmann-Zwicky M,Nöthiger R

    更新日期:1985-10-01 00:00:00

  • Biotech's perfect storm.

    abstract::The global financial crisis has hit biotech companies hard both in the US and Europe as venture capital dries up. Finding new sources of long-term financing for translating research into new therapeutics will be essential for maintaining innovation and new drug development by biotech companies. ...


    pub_type: 杂志文章


    authors: Karberg S

    更新日期:2009-08-07 00:00:00

  • Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments.

    abstract::An easy manipulation of the commercial fluorescence microscope allows stereo pairs of pictures to be taken, which when examined with a stereo viewer, give a strong three-dimensional impression. The procedure is described in detail. Its use allows the documentation by immunofluorescence microscopy of the three-dimensio...


    pub_type: 杂志文章


    authors: Osborn M,Born T,Koitsch HJ,Weber K

    更新日期:1978-07-01 00:00:00

  • Identification of F-actin as the dynamic hub in a microbial-induced GTPase polarity circuit.

    abstract::Polarity in mammalian cells emerges from the assembly of signaling molecules into extensive biochemical interaction networks. Despite their complexity, bacterial pathogens have evolved parsimonious mechanisms to hijack these systems. Here, we develop a tractable experimental and theoretical model to uncover fundamenta...


    pub_type: 杂志文章


    authors: Orchard RC,Kittisopikul M,Altschuler SJ,Wu LF,Süel GM,Alto NM

    更新日期:2012-02-17 00:00:00

  • New telomere formation after developmentally regulated chromosomal breakage during the process of chromatin diminution in Ascaris lumbricoides.

    abstract::During the process of chromatin diminution, which takes place in all presomatic cells of the early Ascaris embryo, the heterochromatic termini of the chromosomes are lost. Here we show that the newly formed ends of the reduced somatic chromosomes carry tandem repeats of the telomeric sequence TTAGGC. Comparison of a c...


    pub_type: 杂志文章


    authors: Müller F,Wicky C,Spicher A,Tobler H

    更新日期:1991-11-15 00:00:00

  • Inherited human Caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune lymphoproliferative syndrome type II.

    abstract::Caspases are cysteine proteases that mediate programmed cell death in phylogenetically diverse multicellular organisms. We report here two kindreds with autoimmune lymphoproliferative syndrome (ALPS) type II, characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects, that harbor...


    pub_type: 杂志文章


    authors: Wang J,Zheng L,Lobito A,Chan FK,Dale J,Sneller M,Yao X,Puck JM,Straus SE,Lenardo MJ

    更新日期:1999-07-09 00:00:00

  • Reduced temperature prevents transfer of a membrane glycoprotein to the cell surface but does not prevent terminal glycosylation.

    abstract::The transport kinetics of the influenza virus hemagglutinin from its site of synthesis to the apical plasma membrane of Madin-Darby canine kidney cells, a polarized epithelial cell line, were studied by a sensitive tryptic assay. Hemagglutinin acquired terminal sugars, as judged by sensitivity to endo-beta-N-acetylglu...


    pub_type: 杂志文章


    authors: Matlin KS,Simons K

    更新日期:1983-08-01 00:00:00