Abstract:
:The HIV-1 trans-activator Tat increases levels of viral gene expression and replication. The target for Tat is an RNA stem-loop called TAR, located at the 5' end of all viral transcripts. To study the mechanism of action and map functional domains of Tat, we fused Tat to the coat protein of bacteriophage MS2, an RNA binding protein. TAR in the HIV-1 LTR was replaced by the operator, the RNA target of the coat protein. The hybrid Tat-coat protein trans-activated HIV-1 LTRs containing either TAR or operator sequences. Mutations in the operator that weaken binding of the coat protein in vitro led to decreased levels of trans-activation in vivo. Deletions in Tat within the hybrid Tat-coat protein identified activation and RNA binding domains of Tat. These experiments suggest that trans-activation by Tat can occur independently of TAR RNA and DNA binding proteins and that Tat exerts its effects on HIV-1 transcription by directly interacting with the TAR RNA stem-loop.
journal_name
Celljournal_title
Cellauthors
Selby MJ,Peterlin BMdoi
10.1016/0092-8674(90)90121-tsubject
Has Abstractpub_date
1990-08-24 00:00:00pages
769-76issue
4eissn
0092-8674issn
1097-4172pii
0092-8674(90)90121-Tjournal_volume
62pub_type
杂志文章相关文献
CELL文献大全abstract::Nine transcripts complementary to mouse L cell mitochondrial DNA have been detected, sized and mapped to restriction fragments using the method of Berk and Sharp (1977). RNA isolated from L cell mitochondria was hybridized to 32P-labeled, cloned L cell mitochondrial DNA restriction fragments in 70% formamide under con...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90309-4
更新日期:1978-05-01 00:00:00
abstract::Lipid biology continues to emerge as an area of significant therapeutic interest, particularly as the result of an enhanced understanding of the wealth of signaling molecules with diverse physiological properties. This growth in knowledge is epitomized by lysophosphatidic acid (LPA), which functions through interactio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.06.002
更新日期:2015-06-18 00:00:00
abstract::Inducer T-cell clones reactive to the p-azobenzenearsonate (arsonate) hapten possess binding sites for radioactive arsanylated proteins, which are not present on clones with other antigen specificities. Binding occurred in the absence of histocompatibility proteins. Binding was specific for the p-azobenzenearsonate ha...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90037-0
更新日期:1984-04-01 00:00:00
abstract::Designing successful microbiota-based therapies requires in-depth understanding of the ecological foundations of this community. In this issue, two studies by Whitaker et al. and Lim et al. provide refined genetic tools for dissecting the spatial organization and temporal dynamics of bacterial communities at the singl...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2017.04.009
更新日期:2017-04-20 00:00:00
abstract::This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90026-6
更新日期:1979-03-01 00:00:00
abstract::Ras-related GTPases are positively regulated by guanine nucleotide exchange factors (GEFs) that promote the exchange of GDP for GTP. The crystal structure of the Sec7 domain GEF bound to nucleotide-free ARF1 GTPase has been determined at 2.8 A resolution and the structure of ARF1 in the GTP-analog form determined at 1...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)81754-7
更新日期:1998-10-16 00:00:00
abstract::A growing body of evidence indicates that plant transcription factors move between cells. A recent paper by Nakajima et al. (2001) shows that movement of the SHORTROOT protein provides a mechanism for signaling positional information between cell layers of the root. ...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/s0092-8674(01)00532-3
更新日期:2001-10-19 00:00:00
abstract::The cellular complexity and scale of the early liver have constrained analyses examining its emergence during organogenesis. To circumvent these issues, we analyzed 45,334 single-cell transcriptomes from embryonic day (E)7.5, when endoderm progenitors are specified, to E10.5 liver, when liver parenchymal and non-paren...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.09.012
更新日期:2020-10-29 00:00:00
abstract::The tumor suppressor p53 exerts antiproliferation effects through its ability to function as a sequence-specific DNA-binding transcription factor. Here, we demonstrate that p53 can be modified by acetylation both in vivo and in vitro. Remarkably, the site of p53 that is acetylated by its coactivator, p300, resides in ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)80521-8
更新日期:1997-08-22 00:00:00
abstract::p53 is a key tumor suppressor protein that has numerous functions. Its primary mode of action has generally been ascribed to the induction of cell-cycle arrest, apoptosis, or senescence upon stress. Li et al. challenge this dogma with evidence that all three of these programs are dispensable for p53's tumor suppressiv...
journal_title:Cell
pub_type: 评论,杂志文章
doi:10.1016/j.cell.2012.05.024
更新日期:2012-06-08 00:00:00
abstract::Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating, perturbing, and engineering cellular systems. Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically dead Cas9 lacking en...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.02.022
更新日期:2013-02-28 00:00:00
abstract::The Hippo-signaling pathway is an important regulator of cellular proliferation and organ size. However, little is known about the role of this cascade in the control of cell fate. Employing a combination of lineage tracing, clonal analysis, and organoid culture approaches, we demonstrate that Hippo pathway activity i...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2014.03.060
更新日期:2014-06-05 00:00:00
abstract::We used clinical tissue from lethal metastatic castration-resistant prostate cancer (CRPC) patients obtained at rapid autopsy to evaluate diverse genomic, transcriptomic, and phosphoproteomic datasets for pathway analysis. Using Tied Diffusion through Interacting Events (TieDIE), we integrated differentially expressed...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.07.007
更新日期:2016-08-11 00:00:00
abstract::Autosomal-dominant dyskeratosis congenita is associated with heterozygous mutations in telomerase. To examine the dosage effect of telomerase, we generated a line of mTR+/- mice on the CAST/EiJ background, which has short telomeres. Interbreeding of heterozygotes resulted in progressive telomere shortening, indicating...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2005.11.020
更新日期:2005-12-16 00:00:00
abstract::During HIV infection, the timing of opportunistic infections is not always associated with severity of CD4 T cell depletion, and different opportunistic pathogens reactivate at different CD4 T cell thresholds. Here, we examine how differences in the phenotype and function of pathogen-specific CD4 T cells influence sus...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.09.045
更新日期:2013-10-24 00:00:00
abstract::Regulated transcription of the prospero gene in the Drosophila eye provides a model for how gene expression is specifically controlled by signals from receptor tyrosine kinases. We show that prospero is controlled by signals from the EGF receptor DER and the Sevenless receptor. A direct link is established between DER...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(00)00107-0
更新日期:2000-09-29 00:00:00
abstract::In normal human fibroblasts, the synthesis of a cell surface receptor for plasma low density lipoprotein (LDL) is regulated by a sensitive system of feedback suppression. The number of functional LDL receptors declines by more than 20 fold when cellular stores of esterified cholesterol are increased by incubation of c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(76)90110-0
更新日期:1976-10-01 00:00:00
abstract::The development of solid tumors is regulated by dynamic interactions between evolving neoplastic cells and their microenvironment. Luo et al. (2007) recently demonstrated that tumor-infiltrating immune cells expressing RANKL induce activation and nuclear localization of IKKalpha in prostatic epithelial tumor cells. Th...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2007.03.029
更新日期:2007-04-06 00:00:00
abstract::Cytotoxic brain edema triggered by neuronal swelling is the chief cause of mortality following brain trauma and cerebral infarct. Using fluorescence lifetime imaging to analyze contributions of intracellular ionic changes in brain slices, we find that intense Na(+) entry triggers a secondary increase in intracellular ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2015.03.029
更新日期:2015-04-23 00:00:00
abstract::The TDS4 gene of S. cerevisiae was isolated as an allele-specific high copy suppressor of mutations within the basic region of the TATA-binding protein (TBP). The gene is essential for viability and encodes a 596 aa protein. The first 300 aa of the TDS4 protein exhibit significant sequence similarity to the RNA polyme...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(92)90351-c
更新日期:1992-10-16 00:00:00
abstract::Cyclic dinucleotides (CDNs) have been previously recognized as important secondary signaling molecules in bacteria and, more recently, in mammalian cells. In the former case, they represent secondary messengers affecting numerous responses of the prokaryotic cell, whereas in the latter, they act as agonists of the inn...
journal_title:Cell
pub_type: 杂志文章,评审
doi:10.1016/j.cell.2013.08.014
更新日期:2013-08-29 00:00:00
abstract::The transmembrane ligand ephrinB2 and its cognate Eph receptor tyrosine kinases are important regulators of vascular morphogenesis. EphrinB2 may have an active signaling role, resulting in bi-directional signal transduction downstream of both ephrinB2 and Eph receptors. To separate the ligand and receptor-like functio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(01)00191-x
更新日期:2001-01-12 00:00:00
abstract::The DNA-binding region of Myb consists of three imperfect tandem repeats (R1, R2, and R3). We have determined the solution structure of a specific DNA complex of the minimum DNA-binding domain (R2R3) by heteronuclear multidimensional NMR. Both R2 and R3 contain three helices, and the third helix in each is found to be...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(94)90549-5
更新日期:1994-11-18 00:00:00
abstract::Large numbers of inbred laboratory rat strains have been developed for a range of complex disease phenotypes. To gain insights into the evolutionary pressures underlying selection for these phenotypes, we sequenced the genomes of 27 rat strains, including 11 models of hypertension, diabetes, and insulin resistance, al...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2013.06.040
更新日期:2013-08-01 00:00:00
abstract::Misfolded proteins of the ER are retrotranslocated to the cytosol, where they are polyubiquitinated, extracted from the membrane, and degraded by the proteasome. To investigate how the ER-associated Degradation (ERAD) machinery can accomplish retrotranslocation of a misfolded luminal protein domain across a lipid bila...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2016.05.048
更新日期:2016-07-14 00:00:00
abstract::Circadian rhythms control organismal physiology throughout the day. At the cellular level, clock regulation is established by a self-sustained Bmal1-dependent transcriptional oscillator network. However, it is still unclear how different tissues achieve a synchronized rhythmic physiology. That is, do they respond inde...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2019.05.009
更新日期:2019-05-30 00:00:00
abstract::We have isolated the Drosophila melanogaster homolog (Dint-1) of int-1, a conserved cellular oncogene implicated in viral mammary tumorigenesis in mice. The deduced Dint-1 protein sequence contains 468 amino acids and starts with a hydrophobic leader; it is 54% identical to the int-1 sequence, and all 23 cysteine resi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(87)90038-9
更新日期:1987-08-14 00:00:00
abstract::Telomeres are thought to be maintained by the preferential recruitment of telomerase to the shortest telomeres. The extension of the G-rich telomeric strand by telomerase is also believed to be coordinated with the complementary synthesis of the C strand by the conventional replication machinery. However, we show that...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2009.05.026
更新日期:2009-08-07 00:00:00
abstract::The non-receptor protein tyrosine phosphatase (PTP) SHP2, encoded by PTPN11, plays an essential role in RAS-mitogen-activated protein kinase (MAPK) signaling during normal development. It has been perplexing as to why both enzymatically activating and inactivating mutations in PTPN11 result in human developmental diso...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/j.cell.2020.09.002
更新日期:2020-10-15 00:00:00
abstract::We have prepared polyclonal antibodies to the cytoplasmic portion of the envelope glycoprotein G of vesicular stomatitis virus (VSV) by using synthetic peptides corresponding to either the 22 or 11 ultimate carboxy-terminal residues of the G as immunogens. When antibodies to the 22 residue peptide are microinjected in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90195-8
更新日期:1984-11-01 00:00:00