Abstract：
:Cellular mRNAs exist in messenger ribonucleoprotein (mRNP) complexes, which undergo transitions during the lifetime of the mRNAs and direct posttranscriptional gene regulation. A final posttranscriptional step in gene expression is the turnover of the mRNP, which involves degradation of the mRNA and recycling of associated proteins. How tightly associated protein components are released from degrading mRNPs is unknown. Here, we demonstrate that the ATPase activity of the RNA helicase Upf1 allows disassembly of mRNPs undergoing nonsense-mediated mRNA decay (NMD). In the absence of Upf1 ATPase activity, partially degraded NMD mRNA intermediates accumulate in complex with NMD factors and concentrate in processing bodies. Thus, disassembly and completion of turnover of mRNPs undergoing NMD requires ATP hydrolysis by Upf1. This uncovers a previously unappreciated and potentially regulated step in mRNA decay and raises the question of how other mRNA decay pathways release protein components of substrate mRNPs.
journal_name
Celljournal_title
Cellauthors
Franks TM,Singh G,Lykke-Andersen Jdoi
10.1016/j.cell.2010.11.043subject
Has Abstractpub_date
2010-12-10 00:00:00pages
938-50issue
6eissn
0092-8674issn
1097-4172pii
S0092-8674(10)01365-6journal_volume
143pub_type
杂志文章相关文献
CELL文献大全abstract：:Pulsed field gradient gel electrophoresis fractionates chromosome-sized DNA molecules from T. brucei. About 60% of the DNA remains in or close to the gel slot (large DNA). There are about three chromosomes of approximately 2 Mb, at least six chromosomes of 200-700 kb, and roughly a hundred mini-chromosomes of 50-150 k...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(84)90302-7
更新日期：1984-05-01 00:00:00
abstract：:We determined the nucleotide sequences of all coding regions and a significant part of the flanking regions of the chicken c-src gene, which is a cellular homolog of the v-src gene of Rous sarcoma virus. The c-src gene consists of 12 exons; the boundaries of the exons were determined by assuming that the amino acid se...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(83)90073-9
更新日期：1983-03-01 00:00:00
abstract：:Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA sequencing to examine ventral midbrain development in human and ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2016.09.027
更新日期：2016-10-06 00:00:00
abstract：:The Bcl-2 family member Bax translocates from the cytosol to mitochondria, where it oligomerizes and permeabilizes the mitochondrial outer membrane to promote apoptosis. Bax activity is counteracted by prosurvival Bcl-2 proteins, but how they inhibit Bax remains controversial because they neither colocalize nor form s...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2011.02.034
更新日期：2011-04-01 00:00:00
abstract：:Loss of cell polarity proteins such as Scribble induces neoplasia in Drosophila by promoting uncontrolled proliferation. In mammals, the role that polarity proteins play during tumorigenesis is not well understood. Here, we demonstrate that depletion of Scribble in mammary epithelia disrupts cell polarity, blocks thre...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2008.09.045
更新日期：2008-11-28 00:00:00
abstract：:The surface glycoproteins T4 and T8 define different functional subsets of T lymphocytes and may act as recognition molecules mediating appropriate interactions between the T cell and its target. Previously we employed gene transfer and subtractive hybridization to isolate a T8 cDNA; now we have isolated and sequenced...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(85)80105-7
更新日期：1985-08-01 00:00:00
abstract：:Condensin protein complexes coordinate the formation of mitotic chromosomes and thereby ensure the successful segregation of replicated genomes. Insights into how condensin complexes bind to chromosomes and alter their topology are essential for understanding the molecular principles behind the large-scale chromatin r...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.09.008
更新日期：2017-10-19 00:00:00
abstract：:Clonal derivatives of C3HMT murine mammary cell lines in culture demonstrate conversion of mammary tumor virus (MMTV) expression at a rate of appriximately 6 per 100 clones. This alteration is largely unidirectional from a relatively high level (MMTV(H)) to a 10 fold lower level (MMTV(L)). This high rate of MMTV(L) va...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(76)90189-6
更新日期：1976-05-01 00:00:00
abstract：:This year, the Albert Lasker Basic Medical Research Award will be shared by Michael Sheetz, James Spudich, and Ronald Vale for discoveries concerning the biophysical actions of cytoskeletal motor-protein machines that move cargo within cells, contract muscles, and enable cell motility. ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.08.031
更新日期：2012-09-14 00:00:00
abstract：:Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C∗ complex) at an average resolution of 3.76 Å. The splicing factor Prp17...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.04.033
更新日期：2017-05-18 00:00:00
abstract：:The increasing proportion of variance in human complex traits explained by polygenic scores, along with progress in preimplantation genetic diagnosis, suggests the possibility of screening embryos for traits such as height or cognitive ability. However, the expected outcomes of embryo screening are unclear, which unde...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2019.10.033
更新日期：2019-11-27 00:00:00
abstract：:Genetic analyses in Drosophila have demonstrated that the multipass membrane protein Smoothened (Smo) is essential for all Hedgehog signaling. We show that Smo acts epistatic to Ptc1 to mediate Shh and Ihh signaling in the early mouse embryo. Smo and Shh/Ihh compound mutants have identical phenotypes: embryos fail to ...
journal_title：Cell
pub_type： 杂志文章
doi：
更新日期：2001-06-15 00:00:00
abstract：:Most mutations in cancer genomes are thought to be acquired after the initiating event, which may cause genomic instability and drive clonal evolution. However, for acute myeloid leukemia (AML), normal karyotypes are common, and genomic instability is unusual. To better understand clonal evolution in AML, we sequenced...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2012.06.023
更新日期：2012-07-20 00:00:00
abstract：:The transcription factor NRF2 is a master regulator of the cellular antioxidant response, and it is often genetically activated in non-small-cell lung cancers (NSCLCs) by, for instance, mutations in the negative regulator KEAP1. While direct pharmacological inhibition of NRF2 has proven challenging, its aberrant activ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2017.08.051
更新日期：2017-10-19 00:00:00
abstract：:The central vasculature of plant roots is protected by a hydrophobic ring of endodermal cells that are enclosed by lamellae of suberin. Barberon et al. demonstrate that endodermal suberization plasticity facilitates ion homeostasis, with antithetical regulation of suberin deposition and degradation by the phytohormone...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2016.01.005
更新日期：2016-01-28 00:00:00
abstract：:The discovery that somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) raised the exciting possibility of modeling diseases with patient-specific cells. Marchetto et al. (2010) now use iPSC technology to generate, characterize, and treat an in vitro model for the autism spectrum disorder Rett...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2010.10.037
更新日期：2010-11-12 00:00:00
abstract：:The achaete-scute gene complex (AS-C), involved in differentiation of the sensory chaetes of D. melanogaster, and the yellow locus have been cloned. The yellow locus is the most distal and is followed, proximally, by the achaete and the scute loci. In the scute locus (75 kb), three transcription units separated by lon...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(85)90147-3
更新日期：1985-02-01 00:00:00
abstract：:Fibrillar protein aggregates are the major pathological hallmark of several incurable, age-related, neurodegenerative disorders. These aggregates typically contain aggregation-prone pathogenic proteins, such as amyloid-beta in Alzheimer's disease and alpha-synuclein in Parkinson's disease. It is, however, poorly under...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.07.020
更新日期：2010-08-20 00:00:00
abstract：:TGF-beta signaling controls a plethora of cellular responses and figures prominently in animal development. Recent cellular, biochemical, and structural studies have revealed significant insight into the mechanisms of the activation of TGF-beta receptors through ligand binding, the activation of Smad proteins through ...
journal_title：Cell
pub_type： 杂志文章,评审
doi：10.1016/s0092-8674(03)00432-x
更新日期：2003-06-13 00:00:00
abstract：:The actions and receptor for ciliary neurotrophic factor (CNTF) are largely restricted to cells of the nervous system, although one of the CNTF receptor components, CNTFR alpha, is expressed by skeletal muscle. Here we show that the other CNTF receptor components, LIFR beta and gp130, are also expressed by skeletal mu...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(94)90113-9
更新日期：1994-02-11 00:00:00
abstract：:Conversion of glial cells into functional neurons represents a potential therapeutic approach for replenishing neuronal loss associated with neurodegenerative diseases and brain injury. Previous attempts in this area using expression of transcription factors were hindered by the low conversion efficiency and failure o...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.03.024
更新日期：2020-04-30 00:00:00
abstract：:The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)00084-2
更新日期：2000-09-01 00:00:00
abstract：:Viable yet damaged cells can accumulate during development and aging. Although eliminating those cells may benefit organ function, identification of this less fit cell population remains challenging. Previously, we identified a molecular mechanism, based on "fitness fingerprints" displayed on cell membranes, which all...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2014.12.017
更新日期：2015-01-29 00:00:00
abstract：:Hematopoietic stem cells (HSCs) exist in the bone marrow and circulate in the blood. In this issue, Massberg et al. (2007) report that HSCs also travel through the lymphatic system. Furthermore, migration of HSCs--which express Toll-like receptors--allows the recognition of pathogenic molecules in peripheral tissues t...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2007.11.010
更新日期：2007-11-30 00:00:00
abstract：:Interaction between CD2 and its counterreceptor, CD58 (LFA-3), on opposing cells optimizes immune recognition, facilitating contacts between helper T lymphocytes and antigen-presenting cells as well as between cytolytic effectors and target cells. Here, we report the crystal structure of the heterophilic adhesion comp...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(00)80790-4
更新日期：1999-06-11 00:00:00
abstract：:The broad expression of the insulin receptor suggests that the spectrum of insulin function has not been fully described. A cell type expressing this receptor is the osteoblast, a bone-specific cell favoring glucose metabolism through a hormone, osteocalcin, that becomes active once uncarboxylated. We show here that i...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2010.06.003
更新日期：2010-07-23 00:00:00
abstract：:We have developed a cell-free system for studying the integration of retroviral DNA. In our assay, amber mutations in a bacteriophage lambda genome that serves as the target for integration are suppressed by integration of an MLV derivative that carries the E. coli supF gene. The structure of the reaction products is ...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/0092-8674(87)90287-x
更新日期：1987-05-08 00:00:00
abstract：:Chronic viral infections represent a major burden to human health, and modulation of the immune system is emerging as a novel approach to fighting such infections. Pellegrini et al. (2011) demonstrate that treatment with the cytokine IL-7 may reinvigorate the immune response to persistent infection by targeting immuno...
journal_title：Cell
pub_type： 评论,杂志文章
doi：10.1016/j.cell.2011.01.038
更新日期：2011-02-18 00:00:00
abstract：:The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison o...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/s0092-8674(85)80084-2
更新日期：1985-07-01 00:00:00
abstract：:Cortical layer 1 (L1) interneurons have been proposed as a hub for attentional modulation of underlying cortex, but the transformations that this circuit implements are not known. We combined genetically targeted voltage imaging with optogenetic activation and silencing to study the mechanisms underlying sensory proce...
journal_title：Cell
pub_type： 杂志文章
doi：10.1016/j.cell.2020.01.001
更新日期：2020-02-06 00:00:00
